##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146545_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3933960
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.789315346368543	31.0	31.0	34.0	27.0	34.0
2	31.161374035323185	31.0	31.0	34.0	28.0	34.0
3	31.270418102878523	31.0	31.0	34.0	28.0	34.0
4	34.8777603229316	37.0	35.0	37.0	32.0	37.0
5	34.76433898666992	37.0	35.0	37.0	32.0	37.0
6	34.94950329947432	37.0	35.0	37.0	32.0	37.0
7	34.90573569634668	37.0	35.0	37.0	32.0	37.0
8	34.90051957823669	37.0	35.0	37.0	32.0	37.0
9	36.520204323378984	39.0	37.0	39.0	32.0	39.0
10-11	36.49880578348534	39.0	37.0	39.0	32.0	39.0
12-13	36.4526286489949	39.0	37.0	39.0	32.0	39.0
14-15	37.7645795839307	40.0	38.0	41.0	32.0	41.0
16-17	37.75909541530672	40.0	38.0	41.0	32.0	41.0
18-19	37.76283973400848	40.0	38.0	41.0	32.0	41.0
20-21	37.83610573061242	40.0	38.0	41.0	32.0	41.0
22-23	37.8272362962511	40.0	38.0	41.0	32.0	41.0
24-25	37.851449430090796	40.0	38.0	41.0	32.0	41.0
26-27	37.844476557972115	40.0	38.0	41.0	32.0	41.0
28-29	37.79066576681004	40.0	38.0	41.0	31.5	41.0
30-31	37.776452480452264	40.0	38.0	41.0	31.0	41.0
32-33	37.76008563889821	40.0	38.0	41.0	31.0	41.0
34-35	37.68784799540412	40.0	38.0	41.0	31.0	41.0
36-37	37.59733334858514	40.0	38.0	41.0	31.0	41.0
38-39	37.547917874101415	40.0	37.5	41.0	30.0	41.0
40-41	37.496449760546625	40.0	37.0	41.0	30.0	41.0
42-43	37.370115989994815	40.0	37.0	41.0	30.0	41.0
44-45	37.22117243184984	40.0	37.0	41.0	30.0	41.0
46-47	37.08642309530346	40.0	36.5	41.0	30.0	41.0
48-49	36.96924727246845	40.0	36.0	41.0	30.0	41.0
50-51	36.84456603524184	40.0	36.0	41.0	30.0	41.0
52-53	36.75312573081577	39.5	35.5	41.0	29.5	41.0
54-55	36.57573106996512	39.0	35.0	41.0	29.0	41.0
56-57	36.413463024535076	39.0	35.0	41.0	29.0	41.0
58-59	36.19754573000234	39.0	35.0	40.5	28.0	41.0
60-61	35.99958146498693	38.5	35.0	40.0	28.0	41.0
62-63	35.7866737333374	38.0	35.0	40.0	28.0	41.0
64-65	35.56048841371036	37.5	34.0	40.0	28.0	41.0
66-67	35.29136163051989	37.0	34.0	40.0	28.0	41.0
68-69	34.9783696326348	36.5	34.0	39.5	27.5	41.0
70-71	34.66072138506746	36.0	34.0	39.0	27.0	41.0
72-73	34.34596691374595	35.5	33.5	39.0	26.5	40.5
74-75	34.03402932414158	35.0	33.0	38.5	26.0	40.0
76-77	33.702209351391474	35.0	33.0	37.0	26.0	39.0
78-79	33.40408392561186	35.0	33.0	37.0	26.0	39.0
80-81	33.08553327944361	35.0	33.0	36.0	26.0	39.0
82-83	32.826392362911676	35.0	33.0	36.0	26.0	37.0
84-85	32.61446138242382	35.0	33.0	35.5	25.5	37.0
86-87	32.44209435276414	35.0	32.0	35.0	25.0	37.0
88-89	32.275500005083934	35.0	32.0	35.0	25.0	36.0
90-91	32.094825570163394	35.0	32.0	35.0	25.0	36.0
92-93	31.954811563920323	35.0	32.0	35.0	25.0	36.0
94-95	31.867677988591648	35.0	32.0	35.0	25.0	35.5
96-97	31.81579184841737	35.0	32.0	35.0	24.0	35.0
98-99	31.76624520839053	35.0	32.0	35.0	24.0	35.0
100	30.750515765284852	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18810610054502774
1101	2	0.30272081406008766
1101	3	0.342824796166294
1101	4	0.3190841149300496
1101	5	0.1960619768603138
1101	6	0.26237940559279593
1101	7	0.2849262625707354
1101	8	0.3795000072517922
1101	9	0.4186356408081622
1101	10-11	0.5021582510221023
1101	12-13	0.4411906488184627
1101	14-15	0.5299877193174183
1101	16-17	0.5175548684775393
1101	18-19	0.47078874017370254
1101	20-21	0.4146213725553807
1101	22-23	0.45599656622109563
1101	24-25	0.47353707744120754
1101	26-27	0.4628468429969175
1101	28-29	0.48323622308321035
1101	30-31	0.4620201130576973
1101	32-33	0.51231267495195
1101	34-35	0.5011856974223363
1101	36-37	0.5067956449897792
1101	38-39	0.4869811070621708
1101	40-41	0.4574879986097997
1101	42-43	0.4346572734716432
1101	44-45	0.3884467874479256
1101	46-47	0.3592790910717696
1101	48-49	0.3179055798411099
1101	50-51	0.36401188540030915
1101	52-53	0.41355774488950203
1101	54-55	0.4903608755153712
1101	56-57	0.3808191813325763
1101	58-59	0.42305361898297633
1101	60-61	0.3645124142233698
1101	62-63	0.37460331491792687
1101	64-65	0.3766057255296005
1101	66-67	0.2771193054886609
1101	68-69	0.30591899882083595
1101	70-71	0.27985084795469817
1101	72-73	0.2580562712607204
1101	74-75	0.30620494082521077
1101	76-77	0.26092848228142174
1101	78-79	0.25638753523629276
1101	80-81	0.25814542539105645
1101	82-83	0.3370443615609915
1101	84-85	0.30163515130983143
1101	86-87	0.3281684279140933
1101	88-89	0.30408444731019557
1101	90-91	0.348691740429679
1101	92-93	0.23123154606073015
1101	94-95	0.2622097885414547
1101	96-97	0.2613831951748402
1101	98-99	0.32837176168122895
1101	100	0.17888024189883467
1102	1	0.3124056174532406
1102	2	0.41625543563818823
1102	3	0.47112592338046255
1102	4	0.4282064981828668
1102	5	0.38479788668318093
1102	6	0.41059196597927183
1102	7	0.47941096466413313
1102	8	0.4847335016157217
1102	9	0.5803989258323199
1102	10-11	0.588961793695205
1102	12-13	0.5856310674980136
1102	14-15	0.7008089753560682
1102	16-17	0.7113672678334169
1102	18-19	0.5995206242316726
1102	20-21	0.6794080070159296
1102	22-23	0.6387703182339877
1102	24-25	0.6596119566682574
1102	26-27	0.6708057801949892
1102	28-29	0.690949588622658
1102	30-31	0.6548099681301593
1102	32-33	0.6149334478988067
1102	34-35	0.6418821547492257
1102	36-37	0.6540017641524187
1102	38-39	0.6600688687368859
1102	40-41	0.6708092546484536
1102	42-43	0.6446250673524787
1102	44-45	0.5959991223915608
1102	46-47	0.5639530041152483
1102	48-49	0.588823454237243
1102	50-51	0.5625787130975652
1102	52-53	0.561637455034429
1102	54-55	0.5748898610226192
1102	56-57	0.5342169269042358
1102	58-59	0.6552838927349853
1102	60-61	0.5947338312926149
1102	62-63	0.47996563375850343
1102	64-65	0.5417707818903139
1102	66-67	0.540533154119899
1102	68-69	0.5107217363409688
1102	70-71	0.4933814437678947
1102	72-73	0.4994089282655523
1102	74-75	0.4854480970248858
1102	76-77	0.576387419479488
1102	78-79	0.4824825432878157
1102	80-81	0.5387533158902471
1102	82-83	0.4958487093870829
1102	84-85	0.5209547970425206
1102	86-87	0.5090661734857491
1102	88-89	0.43115771623129007
1102	90-91	0.5006120302847492
1102	92-93	0.5205069083795699
1102	94-95	0.44028144715659323
1102	96-97	0.449845352984827
1102	98-99	0.4504571078970123
1102	100	0.3132119649262073
1103	1	0.12287195282050334
1103	2	0.18262837113265817
1103	3	0.272113286474827
1103	4	0.14521931681391465
1103	5	0.14623250397644938
1103	6	0.21230130317077567
1103	7	0.1499707837774551
1103	8	0.24010041221380618
1103	9	0.25215114323182775
1103	10-11	0.2942847756694391
1103	12-13	0.31480563223070845
1103	14-15	0.34009982687941687
1103	16-17	0.3614589867461433
1103	18-19	0.33578580005099923
1103	20-21	0.3309402842450382
1103	22-23	0.3966250322610847
1103	24-25	0.3149660591194561
1103	26-27	0.36822211661066007
1103	28-29	0.3954061247965015
1103	30-31	0.41340682078580215
1103	32-33	0.40078921733119444
1103	34-35	0.42744377364307695
1103	36-37	0.35335136191020666
1103	38-39	0.3946018868465231
1103	40-41	0.42772214177599466
1103	42-43	0.393361376788917
1103	44-45	0.3240527034306169
1103	46-47	0.24079313118325274
1103	48-49	0.28262563913585836
1103	50-51	0.2484696219343192
1103	52-53	0.3086894669444291
1103	54-55	0.3920086570218615
1103	56-57	0.32985495783949403
1103	58-59	0.3892911827198162
1103	60-61	0.27132733600699055
1103	62-63	0.2745908250943998
1103	64-65	0.298299145033603
1103	66-67	0.2984620435679446
1103	68-69	0.29397201766588665
1103	70-71	0.28458724430328886
1103	72-73	0.2608142248623935
1103	74-75	0.23994020944302008
1103	76-77	0.22489720119930467
1103	78-79	0.2504968786271249
1103	80-81	0.278714541977628
1103	82-83	0.30093053824368354
1103	84-85	0.28278248290917674
1103	86-87	0.29192983399472183
1103	88-89	0.1828609551372935
1103	90-91	0.2780065596508834
1103	92-93	0.3059054112417421
1103	94-95	0.18793395671465163
1103	96-97	0.18469640804064014
1103	98-99	0.06799126877991668
1103	100	0.13338698047770947
1104	1	0.10299567217606054
1104	2	0.19120388515837305
1104	3	0.18751461399827463
1104	4	0.1700793667687961
1104	5	0.19993033176208996
1104	6	0.19819843754719813
1104	7	0.15653960293838054
1104	8	0.22185619175768778
1104	9	0.3677900750114773
1104	10-11	0.30466288022069676
1104	12-13	0.3015663121645247
1104	14-15	0.34406617259613625
1104	16-17	0.3592689650338343
1104	18-19	0.29862661994901174
1104	20-21	0.33554509163802493
1104	22-23	0.33582738064971096
1104	24-25	0.32565466425808154
1104	26-27	0.3539303340850637
1104	28-29	0.370319879684601
1104	30-31	0.3980296921368236
1104	32-33	0.34552290780839456
1104	34-35	0.3657260361038155
1104	36-37	0.35934992816225986
1104	38-39	0.3089746106672564
1104	40-41	0.3335257666636764
1104	42-43	0.2832443075777178
1104	44-45	0.18404972117021146
1104	46-47	0.19118890704173452
1104	48-49	0.222022359780901
1104	50-51	0.2732524382145485
1104	52-53	0.23456691018551368
1104	54-55	0.2185104891453875
1104	56-57	0.26020114452656884
1104	58-59	0.25731418855543353
1104	60-61	0.2622461240993488
1104	62-63	0.24468964042041108
1104	64-65	0.1765116415936987
1104	66-67	0.24445733526035696
1104	68-69	0.22969647660585935
1104	70-71	0.2854892635791444
1104	72-73	0.25483719379017344
1104	74-75	0.18803949999102088
1104	76-77	0.22869291555371518
1104	78-79	0.25269984838735127
1104	80-81	0.16360203505909254
1104	82-83	0.18251835011989215
1104	84-85	0.20901233530883445
1104	86-87	0.2018378048601619
1104	88-89	0.22327544959634338
1104	90-91	0.24489409071777146
1104	92-93	0.20311932807978295
1104	94-95	0.09155533882127997
1104	96-97	0.18485685270724517
1104	98-99	0.17162611416237894
1104	100	0.16272025265719492
1105	1	0.03217987720111637
1105	2	0.13525035403943875
1105	3	0.1263848894705646
1105	4	0.20734995562447978
1105	5	0.1243400541804931
1105	6	0.17081477453981364
1105	7	0.17265879032621
1105	8	0.17708276380667343
1105	9	0.23514386866433057
1105	10-11	0.27317707758067655
1105	12-13	0.2900767334806247
1105	14-15	0.3730224025035369
1105	16-17	0.2753731800781125
1105	18-19	0.23101358817186224
1105	20-21	0.3392514058237097
1105	22-23	0.2799163653364971
1105	24-25	0.25403954388608696
1105	26-27	0.2344183571518883
1105	28-29	0.31250827131590597
1105	30-31	0.2586043099653992
1105	32-33	0.25270756106210257
1105	34-35	0.2660155955779189
1105	36-37	0.27293511703036444
1105	38-39	0.2400495684339461
1105	40-41	0.2505314768706697
1105	42-43	0.2405744468110953
1105	44-45	0.20497107587539176
1105	46-47	0.23099566022783335
1105	48-49	0.19557220826831667
1105	50-51	0.2088769638549195
1105	52-53	0.2662863849670032
1105	54-55	0.19057807562244022
1105	56-57	0.15566909146986063
1105	58-59	0.2786616900573762
1105	60-61	0.18817294983067967
1105	62-63	0.09681406530351211
1105	64-65	0.18161076039123714
1105	66-67	0.1524612299529906
1105	68-69	0.12039314257569345
1105	70-71	0.17880971504708754
1105	72-73	0.21485307954083765
1105	74-75	0.19511779310619204
1105	76-77	0.09279902897817038
1105	78-79	0.10411012073333836
1105	80-81	0.12203998646582903
1105	82-83	0.17595938074445883
1105	84-85	0.19161564918753982
1105	86-87	0.1614117710622196
1105	88-89	0.17952451971022043
1105	90-91	0.216391127323746
1105	92-93	0.2158279286082454
1105	94-95	0.19310930799896653
1105	96-97	0.16206513213072427
1105	98-99	0.12671675804848448
1105	100	0.16720395163587298
1106	1	0.1539372732041322
1106	2	0.14619817568951277
1106	3	0.18308137935349933
1106	4	0.18791675135226882
1106	5	0.16676023683064045
1106	6	0.20164792369499906
1106	7	0.1572103824894029
1106	8	0.17467298608467985
1106	9	0.20617796754207518
1106	10-11	0.16129413120334135
1106	12-13	0.24230510710121678
1106	14-15	0.19522009795747408
1106	16-17	0.18881345497194957
1106	18-19	0.22549356459705905
1106	20-21	0.23192275288899822
1106	22-23	0.2589804948014489
1106	24-25	0.23254686417142523
1106	26-27	0.21701656957795734
1106	28-29	0.27580270440648746
1106	30-31	0.19835845715693523
1106	32-33	0.21149986177908175
1106	34-35	0.18616563937138864
1106	36-37	0.23192451109791534
1106	38-39	0.20863650824816915
1106	40-41	0.23645012561248535
1106	42-43	0.16399003728621864
1106	44-45	0.06296377059716463
1106	46-47	0.06938475178846915
1106	48-49	0.07250264532077466
1106	50-51	0.10164608450206458
1106	52-53	0.06881285432203299
1106	54-55	0.23641605888542472
1106	56-57	0.10868588396885315
1106	58-59	0.09912988453857707
1106	60-61	0.13526248041743827
1106	62-63	0.08711374360568414
1106	64-65	0.06440943139814692
1106	66-67	0.08278027197197702
1106	68-69	0.07030569206504822
1106	70-71	0.06360387664545186
1106	72-73	0.07256646015110846
1106	74-75	0.040059533694233096
1106	76-77	0.041444296418468696
1106	78-79	0.11941023105832471
1106	80-81	0.07977719654442694
1106	82-83	0.07601618037409708
1106	84-85	0.13127470588621293
1106	86-87	0.06798388385382736
1106	88-89	0.039537489489553934
1106	90-91	0.08326657469472565
1106	92-93	0.11998639821065993
1106	94-95	0.1281299859746241
1106	96-97	-0.002474345203538064
1106	98-99	-0.023054133437394597
1106	100	-0.008106354427273033
1107	1	0.04219006528924041
1107	2	0.1319792186037887
1107	3	0.10819810069598645
1107	4	0.16744945913602294
1107	5	0.1309475610310855
1107	6	0.07731440488417718
1107	7	0.066765583145596
1107	8	0.09584040468633503
1107	9	0.09978929381603763
1107	10-11	0.14021839623635657
1107	12-13	0.17227636422767745
1107	14-15	0.18807670094157913
1107	16-17	0.1646939279682016
1107	18-19	0.18146751813252138
1107	20-21	0.10969919399737904
1107	22-23	0.2079463744835479
1107	24-25	0.1907478920779795
1107	26-27	0.2805498591051645
1107	28-29	0.25188534377751637
1107	30-31	0.15065959976960386
1107	32-33	0.1829104324295443
1107	34-35	0.20603889797710906
1107	36-37	0.14795119857921435
1107	38-39	0.19031354744985407
1107	40-41	0.2188995736430428
1107	42-43	0.19685721301348735
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2216	66-67	-0.886064727009007
2216	68-69	-0.9289630686226502
2216	70-71	-0.9070880002444781
2216	72-73	-0.9253974179780258
2216	74-75	-0.8420064924222359
2216	76-77	-0.8340932151448683
2216	78-79	-0.8792454773132761
2216	80-81	-0.8158606295998041
2216	82-83	-0.7778990222456272
2216	84-85	-0.8090916504782655
2216	86-87	-0.796295944348369
2216	88-89	-0.8262317602727514
2216	90-91	-0.78025422677959
2216	92-93	-0.8272361309733611
2216	94-95	-0.768022289384426
2216	96-97	-0.6702240374508754
2216	98-99	-0.7028066621726374
2216	100	-0.7397373446259934
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	117.0
1	58.0
2	51.0
3	47.0
4	40.0
5	54.0
6	99.0
7	381.0
8	1170.0
9	1952.0
10	2731.0
11	5419.0
12	9110.0
13	11842.0
14	13019.0
15	13229.0
16	13913.0
17	14734.0
18	15691.0
19	16777.0
20	18557.0
21	19785.0
22	22221.0
23	25506.0
24	29819.0
25	35805.0
26	40330.0
27	40612.0
28	44108.0
29	52689.0
30	62329.0
31	72919.0
32	91986.0
33	121692.0
34	180297.0
35	270048.0
36	471356.0
37	886529.0
38	1044744.0
39	281739.0
40	455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.53662389034122	19.35192518899363	12.948041186138823	24.16340973452633
2	14.466733628279663	21.15847149568194	39.94616123614087	24.42863363989753
3	19.32471627807888	25.99368453218837	29.88953651401532	24.792062675717432
4	11.83185478048473	17.21860719101398	40.0781121300313	30.871425898469994
5	12.6608747028089	38.76208339858487	35.6060105310899	12.971031367516325
6	29.785849861014434	37.907976149497316	18.80525292728973	13.50092106219852
7	26.191738742274705	32.60505278313868	23.491420070811362	17.711788403775248
8	24.91807387752124	34.7807054226183	21.208995947100547	19.092224752759915
9	25.655209581903897	16.39605812899675	21.20246640219744	36.74626588690191
10-11	24.290988156379818	26.117293759861955	29.131139068541177	20.460579015217053
12-13	25.718666896136394	24.018171663522477	28.136208416132668	22.12695302420846
14-15	22.74127462751906	25.562118459196093	26.42657777680608	25.270029136478765
16-17	22.060397089969484	28.22130651390964	27.318347443935746	22.399948952185127
18-19	21.844234195781066	28.907180076310823	27.74825613571521	21.500329592192898
20-21	23.049914704562305	27.55922856469914	26.900189275666897	22.490667455071655
22-23	22.51217733076265	27.58440117071167	26.529187145456746	23.37423435306894
24-25	23.048987651245394	27.659418157645376	26.52764917360388	22.76394501750535
26-27	23.038648822816874	27.02349282047306	27.23992724508771	22.697931111622356
28-29	22.228280957893737	28.358263971402092	26.994095147740786	22.419359922963388
30-31	22.94420640246146	27.472327169296594	26.79941880159798	22.784047626643964
32-33	22.624869964195028	27.763343478583625	26.907433319469625	22.70435323775172
34-35	23.055903387613778	27.611194787124877	27.08075001560319	22.252151809658162
36-37	23.120836693047334	27.087610705304126	27.40008794261941	22.391464659029126
38-39	22.97848560482271	27.535303708634157	27.29019962488816	22.19601106165497
40-41	22.693895783896256	27.669233549283344	27.18448058286799	22.452390083952405
42-43	22.636012157659025	27.56804358206496	26.540493069656346	23.255451190619667
44-45	23.01341569587089	27.264016277229576	26.735990333742198	22.98657769315734
46-47	23.096779032978855	27.565614115831234	27.066967813214553	22.27063903797536
48-49	23.136331371870195	27.33573058770026	27.16524946579234	22.362688574637204
50-51	22.05177509489117	27.57693705257735	27.742829731405923	22.628458121125558
52-53	22.392964732760664	28.034616932266992	26.509732674847104	23.062685660125243
54-55	22.261960872468006	27.389691975803178	26.916100052905286	23.432247098823535
56-57	23.144964138408238	26.828811488525933	26.83736858092511	23.188855792140725
58-59	23.455579567787453	27.039431667470442	26.871179341620117	22.63380942312199
60-61	22.991824716469043	27.461059392040056	27.419558683705354	22.127557207785554
62-63	22.41568904469643	27.325660858720607	27.290417264072296	22.968232832510665
64-65	21.894630683302736	28.105248529607902	27.01367653141183	22.986444255677533
66-67	22.870070387032683	27.111563300585907	26.632958433392336	23.385407878989078
68-69	22.6184509228157	27.184441330120002	26.81331625456118	23.383791492503118
70-71	22.963686585412994	27.076518115256526	26.723881439618186	23.235913859712294
72-73	22.99226848591426	26.629115039218277	27.516162257918957	22.862454216948507
74-75	22.639035091121055	27.79618523746174	26.809696845477486	22.755082825939713
76-77	22.65344598982918	26.92496947177137	26.987335553167433	23.434248985232017
78-79	22.77082915873705	27.14404722065975	27.197117570532452	22.888006050070743
80-81	22.856302195433102	27.053832557298552	27.226531501536073	22.863333745732277
82-83	22.668940052791385	26.75072346571866	27.61874246654766	22.961594014942303
84-85	22.63406318386934	27.424066504839313	27.000960691005865	22.94090962028548
86-87	22.46081199803186	27.439077262249707	26.976068269574295	23.12404247014414
88-89	22.605230319554355	27.066035978709515	26.7425721363654	23.58616156537073
90-91	22.95540986649596	26.58711899919965	26.95726357719122	23.50020755711317
92-93	23.039975995204127	27.15440817943076	27.12915737190714	22.67645845345797
94-95	22.413209320280014	27.046254159794863	27.42263668406004	23.117899835865078
96-97	22.08387262468119	27.03895357559699	27.82599685197946	23.05117694774235
98-99	22.611638183886143	27.311074478345432	26.7954399072196	23.281847430548826
100	22.4009713119704	26.399852523313395	26.7660778193792	24.433098345337005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	981.0
1	683.5
2	402.5
3	404.0
4	348.5
5	276.0
6	244.5
7	239.5
8	259.5
9	256.0
10	234.5
11	234.5
12	258.0
13	297.5
14	328.0
15	401.5
16	507.0
17	644.5
18	868.5
19	1207.5
20	1729.5
21	2425.0
22	3438.0
23	5011.0
24	7536.0
25	10715.0
26	15352.0
27	21034.0
28	26756.0
29	33884.5
30	41875.0
31	49781.5
32	58396.0
33	67278.5
34	76073.5
35	85487.5
36	95097.0
37	106154.5
38	113698.5
39	116255.5
40	118101.0
41	121036.5
42	125411.0
43	130407.5
44	136076.5
45	143596.5
46	153390.0
47	175053.0
48	214706.5
49	217435.5
50	193963.0
51	175202.5
52	148673.5
53	132578.5
54	121580.0
55	109451.5
56	98463.0
57	86583.0
58	76272.0
59	68707.5
60	56716.0
61	43601.0
62	33787.5
63	26324.0
64	19748.5
65	14142.5
66	10599.0
67	8016.0
68	5975.5
69	4464.5
70	3555.5
71	2951.0
72	2426.0
73	2117.5
74	1721.5
75	1296.5
76	971.0
77	675.0
78	465.5
79	296.0
80	200.5
81	167.5
82	121.5
83	72.0
84	57.5
85	45.0
86	35.0
87	35.0
88	28.5
89	17.5
90	11.0
91	7.0
92	7.0
93	9.0
94	6.0
95	4.0
96	5.5
97	5.5
98	3.5
99	5.5
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05963456669615349
2	0.009227343440197664
3	0.011260917752086956
4	0.009761156697068602
5	0.011794731008957894
6	0.011642212935566198
7	0.0032537188990228675
8	0.005922785183377564
9	0.007498805275091765
10-11	0.0071429297705111385
12-13	0.006431178761349886
14-15	0.01024413059614231
16-17	0.009989933807156149
18-19	0.007905520137469623
20-21	0.0071048002521632144
22-23	0.0028851335549929333
24-25	0.004130697821025125
26-27	0.005211034174216311
28-29	0.006253241009059574
30-31	0.009240053279646972
32-33	0.010879622568607713
34-35	0.011909119564001668
36-37	0.018136940894162623
38-39	0.01863262463268564
40-41	0.018289458967554323
42-43	0.0245554098160632
44-45	0.028063325504072235
46-47	0.030821360664572084
48-49	0.03219402332509736
50-51	0.03296932353150515
52-53	0.03056716387558592
54-55	0.03712544103142889
56-57	0.03938779245340573
58-59	0.0374558968571109
60-61	0.038053259311228375
62-63	0.03382088277460879
64-65	0.036108653875484245
66-67	0.03783719204059014
68-69	0.04049354848549553
70-71	0.04026477137540799
72-73	0.03605781451768701
74-75	0.03880313983873755
76-77	0.03899378743047718
78-79	0.03670601632960172
80-81	0.04279402942582029
82-83	0.03812951834792423
84-85	0.03492663880669859
86-87	0.03342687775168024
88-89	0.030948459059065164
90-91	0.03323623015994062
92-93	0.03590529644429531
94-95	0.038752300480940326
96-97	0.03380817293515949
98-99	0.03134246408199372
100	0.02885133554992933
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3933960.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.670669807402724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.83624047518437	46.90037316447667
2	13.834802738930208	17.340727086040918
3	5.245222662485709	9.861648526408429
4	2.4367770282975822	6.108577941388073
5	1.244543966699272	3.8998201998902635
6	0.7339261817331986	2.7597387239045474
7	0.4751621541618775	2.084511132791725
8	0.31233166289906544	1.5659227612755475
9	0.2100381935068403	1.1846910844989493
>10	0.6598756911813817	6.065881896920571
>50	0.008591723504954726	0.35460173481120894
>100	0.0019300199271541517	0.19080508645430105
>500	1.2388921963945963E-4	0.0601338868176576
>1k	3.097230490974728E-4	0.46177700690374957
>5k	6.194460981949456E-5	0.22116381743006816
>10k+	6.194460981949456E-5	0.9396259499872512
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	24204	0.6152579080621053	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	5697	0.14481591068541624	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	4631	0.1177185329794914	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	4071	0.10348351279626634	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.5419678898616152E-5	0.0
3	0.0	0.0	0.0	2.5419678898616152E-5	0.0
4	0.0	0.0	0.0	2.5419678898616152E-5	0.0
5	0.0	0.0	0.0	2.5419678898616152E-5	0.0
6	0.0	0.0	0.0	2.5419678898616152E-5	0.0
7	0.0	0.0	0.0	2.5419678898616152E-5	0.0
8	0.0	0.0	0.0	2.5419678898616152E-5	0.0
9	0.0	0.0	0.0	5.0839357797232305E-5	0.0
10-11	0.0	0.0	0.0	5.0839357797232305E-5	0.0
12-13	0.0	0.0	0.0	5.0839357797232305E-5	0.0
14-15	0.0	0.0	0.0	5.0839357797232305E-5	0.0
16-17	0.0	0.0	0.0	5.0839357797232305E-5	0.0
18-19	0.0	0.0	0.0	1.2709839449308075E-4	0.0
20-21	0.0	0.0	0.0	2.0335743118892922E-4	0.0
22-23	0.0	0.0	0.0	4.829738990737069E-4	0.0
24-25	0.0	0.0	0.0	8.642690825529493E-4	0.0
26-27	0.0	0.0	0.0	0.0016776988073086661	0.0
28-29	0.0	0.0	0.0	0.004130697821025125	0.0
30-31	0.0	0.0	0.0	0.01049832738512847	0.0
32-33	0.0	0.0	0.0	0.027173636742620667	0.0
34-35	0.0	0.0	0.0	0.05308899937975983	0.0
36-37	0.0	0.0	0.0	0.0925530508698614	0.0
38-39	0.0	0.0	0.0	0.15001423502018324	0.0
40-41	0.0	0.0	0.0	0.21400827664744937	0.0
42-43	0.0	0.0	0.0	0.2733759367151674	0.0
44-45	0.0	0.0	0.0	0.3452754984799032	0.0
46-47	0.0	0.0	0.0	0.43215996095537323	0.0
48-49	0.0	0.0	0.0	0.5169600097611566	0.0
50-51	0.0	0.0	0.0	0.6065135385209814	0.0
52-53	0.0	0.0	0.0	0.6945927259046865	0.0
54-55	0.0	0.0	0.0	0.7898275528983518	0.0
56-57	0.0	0.0	0.0	0.9021571139513366	0.0
58-59	0.0	0.0	0.0	1.021896511403268	0.0
60-61	0.0	0.0	0.0	1.1429831518368259	0.0
62-63	0.0	0.0	0.0	1.2633834609401213	0.0
64-65	0.0	0.0	0.0	1.3853724999745802	0.0
66-67	0.0	0.0	0.0	1.5240114286876327	0.0
68-69	0.0	0.0	0.0	1.6802280653590784	0.0
70-71	0.0	0.0	0.0	1.852077804553173	0.0
72-73	0.0	0.0	0.0	2.0136961229905745	0.0
74-75	0.0	0.0	0.0	2.1772209173453723	0.0
76-77	0.0	0.0	0.0	2.349579050117439	0.0
78-79	0.0	0.0	0.0	2.5469247272468456	0.0
80-81	0.0	0.0	0.0	2.753459618298102	0.0
82-83	0.0	0.0	0.0	2.9656758075831986	0.0
84-85	0.0	0.0	0.0	3.1782351625334266	0.0
86-87	0.0	0.0	0.0	3.395420899043203	0.0
88	0.0	0.0	0.0	3.57108359007209	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1650	0.0	27.069439	1
GCATCGT	1425	0.0	23.742134	8
TAGGCAT	1960	0.0	21.817173	5
TATACCG	495	0.0	20.884815	5
CATGGGG	7035	0.0	19.905144	4
CGACGAG	840	0.0	19.019012	24-25
CTAGTAC	2155	0.0	18.752724	3
TAGTACT	2440	0.0	18.680782	4
GTATAAG	1790	0.0	18.385887	1
GTATATA	2415	0.0	18.299967	1
CAATGCG	940	0.0	17.995918	18-19
TACCTGG	3950	0.0	17.963585	2
CATCGTC	2055	0.0	17.835484	9
GTACCTG	4230	0.0	17.78358	1
CTGTGCG	2025	0.0	17.171522	9
CTAGGCA	2855	0.0	17.117502	4
TGCGACG	940	0.0	16.994957	22-23
GTGATCG	925	0.0	16.763872	8
ACATGGG	23065	0.0	16.604176	3
TACTAGG	2610	0.0	16.56382	2
>>END_MODULE