FastQCFastQC Report
Mon 16 Jan 2017
ERR1146545_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146545_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3933960
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT256090.650972556914661No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA114560.29120784146254663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73270.18624998729016054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69810.17745477839123935No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA45220.11494778797954223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15350.020.8498021
GAGTACT93650.020.3743812-13
CATGGGG66000.019.5918624
TAGTACT25250.019.5530684
CTAACGC4100.019.4962943
CTAGTAC22300.018.344313
GGCGTCG7550.018.0516628
AGTACTT104950.018.00154312-13
GTACTTT106350.017.83085614-15
GTACCTG38950.017.7627931
ATGGGGG46150.017.7281975
TACGGGC7500.017.554314
TACCTGG38100.017.540082
CTATTGA21200.017.291199
GGGCTAT23500.017.199626
TACGCCG1650.001567636817.0983545
GTCGACG3300.017.09020436-37
CTGTGCG23700.017.0536069
ACGTGCG9950.017.0037849
CGGGCGT8250.016.5208456
Produced by FastQC (version 0.11.5)