FastQCFastQC Report
Mon 16 Jan 2017
ERR1146544_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146544_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4467153
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45450.038.8941881
GTATCAA71100.037.1618581
CAACGCA76100.031.8637125
ATCAACG78100.031.2282493
TATCAAC92500.028.0432742
AACGCAG90800.027.8942586
TCAACGC94100.026.068274
GTACTAG20750.025.8295881
ACGCAGA96150.025.217257
CGCAGAG98250.024.5842558
GTACATG151550.024.4456181
TACATGG150050.023.9583442
ACATGGG151850.022.9006753
GCATCGT21400.022.6177798
GTGATCG14950.022.3174028
TGATCGC15450.020.9868419
CATGGGG119200.020.7367554
TAGGCAT29800.020.657915
GTATATA19800.020.1829411
GTATAAG26350.020.1617641
Produced by FastQC (version 0.11.5)