##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146544_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4467153
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.605923728155272	33.0	31.0	34.0	29.0	34.0
2	31.745315416776634	33.0	31.0	34.0	30.0	34.0
3	31.784777015696573	34.0	31.0	34.0	29.0	34.0
4	35.276942383661364	37.0	35.0	37.0	33.0	37.0
5	35.26239463927025	37.0	35.0	37.0	33.0	37.0
6	35.35608831844354	37.0	35.0	37.0	33.0	37.0
7	35.212427243929184	37.0	35.0	37.0	32.0	37.0
8	35.232511400437815	37.0	35.0	37.0	32.0	37.0
9	36.841125656542324	39.0	37.0	39.0	33.0	39.0
10-11	36.820523944445156	39.0	37.5	39.0	33.0	39.0
12-13	36.80481975880387	39.0	38.0	39.0	33.0	39.0
14-15	38.240384983455904	41.0	39.0	41.0	33.0	41.0
16-17	38.263059604181905	41.0	39.0	41.0	33.0	41.0
18-19	38.300086542815976	41.0	38.5	41.0	33.5	41.0
20-21	38.382349227796766	41.0	39.0	41.0	34.0	41.0
22-23	38.40715104228577	41.0	39.0	41.0	34.0	41.0
24-25	38.46593434341739	41.0	39.0	41.0	33.5	41.0
26-27	38.49789720656534	41.0	39.0	41.0	33.5	41.0
28-29	38.46512342424806	41.0	39.0	41.0	33.0	41.0
30-31	38.4648532297864	40.0	39.0	41.0	33.0	41.0
32-33	38.47737071015925	40.0	38.0	41.0	33.0	41.0
34-35	38.424190754155944	40.0	38.0	41.0	33.0	41.0
36-37	38.34530124667769	40.0	38.0	41.0	33.0	41.0
38-39	38.3056603389228	40.0	38.0	41.0	33.0	41.0
40-41	38.24131577763286	40.0	38.0	41.0	33.0	41.0
42-43	38.13580539999414	40.0	38.0	41.0	33.0	41.0
44-45	38.02236222936622	40.0	38.0	41.0	33.0	41.0
46-47	37.92675547490762	40.0	38.0	41.0	32.5	41.0
48-49	37.81483777251417	40.0	37.0	41.0	32.0	41.0
50-51	37.69181075732128	40.0	37.0	41.0	32.0	41.0
52-53	37.566828358016835	40.0	37.0	41.0	32.0	41.0
54-55	37.3908074113423	40.0	36.0	41.0	31.0	41.0
56-57	37.25996367261206	39.5	36.0	41.0	31.0	41.0
58-59	37.038543788403935	39.0	35.0	41.0	31.0	41.0
60-61	36.86126734410037	39.0	35.0	41.0	31.0	41.0
62-63	36.623833121453416	39.0	35.0	41.0	31.0	41.0
64-65	36.375848666924995	38.0	35.0	40.0	31.0	41.0
66-67	36.11565867567106	37.0	35.0	40.0	30.5	41.0
68-69	35.82232341269708	37.0	35.0	40.0	30.0	41.0
70-71	35.55454917259382	36.0	35.0	39.0	30.0	41.0
72-73	35.239153214586565	36.0	35.0	39.0	30.0	41.0
74-75	34.91648987621422	35.0	34.5	39.0	29.5	40.5
76-77	34.60537639968902	35.0	34.0	37.0	29.0	39.5
78-79	34.32756993100527	35.0	34.0	37.0	29.0	39.0
80-81	34.05179484562092	35.0	34.0	36.5	29.0	39.0
82-83	33.795603710013964	35.0	34.0	36.0	29.0	37.5
84-85	33.57180098823569	35.0	34.0	36.0	29.0	37.0
86-87	33.382373068484554	35.0	34.0	35.5	29.0	37.0
88-89	33.24338454492157	35.0	34.0	35.0	29.0	36.0
90-91	33.08789423599326	35.0	34.0	35.0	28.0	36.0
92-93	32.99700749000537	35.0	34.0	35.0	28.0	36.0
94-95	32.90551308629904	35.0	34.0	35.0	28.0	36.0
96-97	32.831939940270686	35.0	34.0	35.0	28.0	35.5
98-99	32.76764574663102	35.0	34.0	35.0	27.5	35.0
100	31.905190621409208	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2858823419062304
1101	2	0.31591242802208086
1101	3	0.33584555253759873
1101	4	0.32287855913182284
1101	5	0.33896454957691446
1101	6	0.3574110152424339
1101	7	0.3071511360909156
1101	8	0.4083759266123934
1101	9	0.46809039312601186
1101	10-11	0.5150854330193653
1101	12-13	0.47295237824587133
1101	14-15	0.596572547388412
1101	16-17	0.5504016239446798
1101	18-19	0.5172688663919658
1101	20-21	0.471146562095619
1101	22-23	0.4945392920032816
1101	24-25	0.4864955531664563
1101	26-27	0.47657301870656
1101	28-29	0.5518848909222527
1101	30-31	0.5020865100589234
1101	32-33	0.5329209456690265
1101	34-35	0.5599959410675268
1101	36-37	0.554029484453288
1101	38-39	0.5275266303184409
1101	40-41	0.5075522837082858
1101	42-43	0.5395001118968707
1101	44-45	0.5012930023474667
1101	46-47	0.4700187225400114
1101	48-49	0.4631219777564439
1101	50-51	0.4891595105448161
1101	52-53	0.47886270521269836
1101	54-55	0.4635006656746228
1101	56-57	0.45766502910271356
1101	58-59	0.4151837128654492
1101	60-61	0.40560059751907573
1101	62-63	0.4444658279093261
1101	64-65	0.38507419429782175
1101	66-67	0.3747747634354681
1101	68-69	0.40262498145794723
1101	70-71	0.3791838973724353
1101	72-73	0.3350028287056901
1101	74-75	0.3361648594813147
1101	76-77	0.3383452199399457
1101	78-79	0.3485931963205431
1101	80-81	0.354655603070519
1101	82-83	0.27482776449966195
1101	84-85	0.29491654643984333
1101	86-87	0.3403040867294891
1101	88-89	0.3059361368338216
1101	90-91	0.35750567697162694
1101	92-93	0.3079145490369086
1101	94-95	0.27324153071718627
1101	96-97	0.2687974756087286
1101	98-99	0.33376103866307716
1101	100	0.17690560768447838
1102	1	0.178349441637625
1102	2	0.1779688759172373
1102	3	0.23272585078803587
1102	4	0.15669788800708062
1102	5	0.17222997341091428
1102	6	0.1926260880268984
1102	7	0.17590856991437676
1102	8	0.26216043387337606
1102	9	0.27182903007790316
1102	10-11	0.28870801426035086
1102	12-13	0.3039351607219771
1102	14-15	0.3948252178980809
1102	16-17	0.3432232804892408
1102	18-19	0.2837444399546243
1102	20-21	0.3250488924911039
1102	22-23	0.3340187719702925
1102	24-25	0.30449830936706945
1102	26-27	0.3617546268414813
1102	28-29	0.37308441711795126
1102	30-31	0.3248369121707597
1102	32-33	0.33712523900743463
1102	34-35	0.3403473214192303
1102	36-37	0.347424726622485
1102	38-39	0.2998971129353549
1102	40-41	0.3149544803983346
1102	42-43	0.29007352904957173
1102	44-45	0.27371782481634455
1102	46-47	0.22538657485078062
1102	48-49	0.25098039027923846
1102	50-51	0.2619416006932198
1102	52-53	0.2004943006203277
1102	54-55	0.23314170408652757
1102	56-57	0.2174584855920898
1102	58-59	0.22690099615465442
1102	60-61	0.1681512041864579
1102	62-63	0.15641281500346338
1102	64-65	0.15665556975771722
1102	66-67	0.1672767215386486
1102	68-69	0.18067577053447081
1102	70-71	0.10832567997076126
1102	72-73	0.14877459642640645
1102	74-75	0.12314533419842633
1102	76-77	0.10089431036004726
1102	78-79	0.13341810345422545
1102	80-81	0.10823500342687709
1102	82-83	0.1536556226487562
1102	84-85	0.1966795897656155
1102	86-87	0.120875675196082
1102	88-89	0.0702273800616382
1102	90-91	0.12528461056886897
1102	92-93	0.14958546579064347
1102	94-95	0.10890559648372289
1102	96-97	0.11718192179097997
1102	98-99	0.1211980263361383
1102	100	0.030071026586846017
1103	1	0.09530175877730329
1103	2	0.12627456003372473
1103	3	0.16070003973153746
1103	4	0.05865723340692597
1103	5	0.14886737486312285
1103	6	0.14318548317702096
1103	7	0.0953235846887921
1103	8	0.1885094078182945
1103	9	0.2472419366696954
1103	10-11	0.2695676599934984
1103	12-13	0.24984083727547812
1103	14-15	0.24700672604040363
1103	16-17	0.24432363600872264
1103	18-19	0.2223202231521455
1103	20-21	0.24003976668377192
1103	22-23	0.2604157531567708
1103	24-25	0.25227638651308126
1103	26-27	0.24448501876373996
1103	28-29	0.3204031905196274
1103	30-31	0.2887732223666788
1103	32-33	0.26185171590221756
1103	34-35	0.26556763772934033
1103	36-37	0.3055560011689522
1103	38-39	0.24383833316421288
1103	40-41	0.2267537150004202
1103	42-43	0.22496330146866228
1103	44-45	0.1863284556580851
1103	46-47	0.12947655548835968
1103	48-49	0.18837165018423718
1103	50-51	0.200325502639096
1103	52-53	0.17255272705816793
1103	54-55	0.24011424630779032
1103	56-57	0.25307870665583465
1103	58-59	0.25866610277800106
1103	60-61	0.2616577003786702
1103	62-63	0.2698215477718193
1103	64-65	0.212818547400218
1103	66-67	0.17434657813141996
1103	68-69	0.17547420806863556
1103	70-71	0.18901808324577019
1103	72-73	0.24053543114382592
1103	74-75	0.24330329427176878
1103	76-77	0.2283265049320633
1103	78-79	0.13580620522130005
1103	80-81	0.16388623864582286
1103	82-83	0.1824228025940755
1103	84-85	0.16476416877608102
1103	86-87	0.14305375045135094
1103	88-89	0.08338993169527242
1103	90-91	0.13278564604814846
1103	92-93	0.12397728051164592
1103	94-95	0.1112093532202465
1103	96-97	0.0678284685488535
1103	98-99	0.02959991401041151
1103	100	0.054887550295333654
1104	1	0.2283455970989401
1104	2	0.28115885724658085
1104	3	0.25421031589441156
1104	4	0.2352177603289647
1104	5	0.2143235317384793
1104	6	0.2562681871055901
1104	7	0.2104270069418206
1104	8	0.23905298197447422
1104	9	0.29682937510060725
1104	10-11	0.3055063810214591
1104	12-13	0.31227859829159854
1104	14-15	0.3937426352355615
1104	16-17	0.369702294236923
1104	18-19	0.3219633933331849
1104	20-21	0.3373554579774023
1104	22-23	0.33809270146342385
1104	24-25	0.26190890412706125
1104	26-27	0.2864188211740384
1104	28-29	0.34642993468157357
1104	30-31	0.359434112952286
1104	32-33	0.306886660737554
1104	34-35	0.375810036294439
1104	36-37	0.34701986712558863
1104	38-39	0.3100464979077415
1104	40-41	0.30684762982470914
1104	42-43	0.2865656339443632
1104	44-45	0.27248776959299903
1104	46-47	0.2861550291293682
1104	48-49	0.26297909693700205
1104	50-51	0.21340114530269005
1104	52-53	0.23075663616772601
1104	54-55	0.30952273138669995
1104	56-57	0.24592197134658988
1104	58-59	0.18452471636687306
1104	60-61	0.2248923563256966
1104	62-63	0.20796945885919627
1104	64-65	0.20428339081119162
1104	66-67	0.20229435712764143
1104	68-69	0.21254867205711037
1104	70-71	0.20106107910287108
1104	72-73	0.19149658896208877
1104	74-75	0.1482550021288631
1104	76-77	0.2060572300801624
1104	78-79	0.21430029693112118
1104	80-81	0.22307354265991108
1104	82-83	0.24615147744742671
1104	84-85	0.2226960759361205
1104	86-87	0.17537319398996232
1104	88-89	0.18927697066755655
1104	90-91	0.19745478365197755
1104	92-93	0.17893132246229726
1104	94-95	0.15008060579933158
1104	96-97	0.1281917933833867
1104	98-99	0.12224295223511206
1104	100	0.08635172128512991
1105	1	0.12206044252528159
1105	2	0.15049424909727804
1105	3	0.1723784007946776
1105	4	0.1305870166310683
1105	5	0.10198964945678313
1105	6	0.0589290501135693
1105	7	0.005068762366057911
1105	8	0.09934679336554808
1105	9	0.09389873337701005
1105	10-11	0.14661172497683594
1105	12-13	0.1521009957904269
1105	14-15	0.23389597730776757
1105	16-17	0.17652051682667036
1105	18-19	0.18449293304177417
1105	20-21	0.22211978535030852
1105	22-23	0.1779314784956938
1105	24-25	0.17826159073713654
1105	26-27	0.2225847374240857
1105	28-29	0.29251482839946163
1105	30-31	0.26978631348020343
1105	32-33	0.22516269456301075
1105	34-35	0.2377639523990922
1105	36-37	0.21790889672247005
1105	38-39	0.2203804079566467
1105	40-41	0.23717100549368553
1105	42-43	0.1983514726365314
1105	44-45	0.1538105229956912
1105	46-47	0.15089561085863323
1105	48-49	0.1832857249230102
1105	50-51	0.1565824547262693
1105	52-53	0.18304512218248448
1105	54-55	0.20137596483919395
1105	56-57	0.18333203015848198
1105	58-59	0.19684001957777753
1105	60-61	0.11403341203186557
1105	62-63	0.1031689529968105
1105	64-65	0.11923367736723378
1105	66-67	0.08838679167072883
1105	68-69	0.09182842965249449
1105	70-71	0.060056968463960914
1105	72-73	0.06406826463047821
1105	74-75	0.08977519286774793
1105	76-77	0.028873966968227194
1105	78-79	0.05731319355177078
1105	80-81	0.07225294363384904
1105	82-83	0.06254934817047797
1105	84-85	0.06706613389938099
1105	86-87	0.038688093328367756
1105	88-89	0.03626202114537591
1105	90-91	0.09503059710250739
1105	92-93	0.06498553809402097
1105	94-95	0.025970423953751265
1105	96-97	0.005586773664774114
1105	98-99	0.018055235918026824
1105	100	0.014433479804321081
1106	1	0.18271569370790175
1106	2	0.20886415514736711
1106	3	0.23250664512440977
1106	4	0.23940553801411824
1106	5	0.24983635581384078
1106	6	0.23778597284920977
1106	7	0.17307378062243828
1106	8	0.16685793005757432
1106	9	0.26866056105355796
1106	10-11	0.29017777470374284
1106	12-13	0.32051888104802373
1106	14-15	0.36770460607070987
1106	16-17	0.29970784408054385
1106	18-19	0.3120697357469524
1106	20-21	0.329866681166024
1106	22-23	0.3067079112768454
1106	24-25	0.3639154929261039
1106	26-27	0.33501518930697927
1106	28-29	0.37040699584255066
1106	30-31	0.3341835981058736
1106	32-33	0.30920709743846686
1106	34-35	0.31347487205302826
1106	36-37	0.31066217777480176
1106	38-39	0.2887910985814415
1106	40-41	0.31532090566815185
1106	42-43	0.25689052750160357
1106	44-45	0.2852178681162343
1106	46-47	0.25899039714806094
1106	48-49	0.3324644126219809
1106	50-51	0.33309467660874503
1106	52-53	0.27155184616639616
1106	54-55	0.2814526026673789
1106	56-57	0.2234026125040458
1106	58-59	0.2916801938077995
1106	60-61	0.2389635641383947
1106	62-63	0.2876490808214527
1106	64-65	0.2768111245916032
1106	66-67	0.30911969992118316
1106	68-69	0.28917256093055954
1106	70-71	0.23337155078554872
1106	72-73	0.21653444985630443
1106	74-75	0.2015273257765955
1106	76-77	0.2089817815385615
1106	78-79	0.17755326945515293
1106	80-81	0.22310580739748076
1106	82-83	0.2134106806164766
1106	84-85	0.23439987792634298
1106	86-87	0.14642801934355987
1106	88-89	0.21162639931925753
1106	90-91	0.2164655066599721
1106	92-93	0.19642795704947957
1106	94-95	0.1776352320069492
1106	96-97	0.21145009962361172
1106	98-99	0.18831839460164446
1106	100	0.05443803640509515
1107	1	0.01302386222191032
1107	2	0.03315366816221399
1107	3	0.13577553011883126
1107	4	0.08927903112167712
1107	5	-0.039667175757884365
1107	6	0.07660082896895659
1107	7	0.007734962162672332
1107	8	0.041010125246138784
1107	9	0.06611335330914159
1107	10-11	0.07718037691448387
1107	12-13	0.019083302833763582
1107	14-15	0.09442923451599228
1107	16-17	0.11848311700920533
1107	18-19	0.05840843282079078
1107	20-21	0.08155783364950508
1107	22-23	0.06871610862000921
1107	24-25	0.07873377440488838
1107	26-27	0.10396220435124803
1107	28-29	0.13006836704829539
1107	30-31	0.10946069941001468
1107	32-33	0.11226099658193078
1107	34-35	0.1362251546546318
1107	36-37	0.08173230849227053
1107	38-39	0.10956117846855307
1107	40-41	0.06371055069018894
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2216	44-45	-0.38388164147045245
2216	46-47	-0.2817877254367147
2216	48-49	-0.30333805221876275
2216	50-51	-0.3155617179256254
2216	52-53	-0.2893586548302025
2216	54-55	-0.2329668923325059
2216	56-57	-0.24317996062441694
2216	58-59	-0.26330364403323614
2216	60-61	-0.2873949564005187
2216	62-63	-0.358653782943982
2216	64-65	-0.2979133925774988
2216	66-67	-0.25380207908310837
2216	68-69	-0.2821761363555453
2216	70-71	-0.30591204934204796
2216	72-73	-0.27139542896050983
2216	74-75	-0.2846758746127591
2216	76-77	-0.27621084235110516
2216	78-79	-0.2665557850647886
2216	80-81	-0.24153265907607846
2216	82-83	-0.25407610990952634
2216	84-85	-0.23798401643497158
2216	86-87	-0.24880882702476015
2216	88-89	-0.26275278751664644
2216	90-91	-0.22028655415356013
2216	92-93	-0.22690340128551867
2216	94-95	-0.18089834867396348
2216	96-97	-0.18270358414972065
2216	98-99	-0.1983566020264007
2216	100	-0.2434954052587699
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	120.0
1	73.0
2	54.0
3	50.0
4	43.0
5	74.0
6	195.0
7	1134.0
8	3024.0
9	3581.0
10	3343.0
11	4034.0
12	5240.0
13	6288.0
14	7472.0
15	8502.0
16	9260.0
17	10067.0
18	11114.0
19	12666.0
20	14289.0
21	16309.0
22	18110.0
23	22093.0
24	28067.0
25	36232.0
26	40217.0
27	36737.0
28	38554.0
29	49358.0
30	54758.0
31	59897.0
32	75129.0
33	100187.0
34	153113.0
35	242306.0
36	452329.0
37	1049812.0
38	1394978.0
39	497450.0
40	894.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17460157466205	20.51712976962448	13.21045145538644	24.09781720032703
2	14.9259080987295	21.055263894330327	40.41132814686293	23.607499860077237
3	17.545391858793817	26.711166733084674	30.766993682080123	24.976447726041382
4	12.148254315155269	17.816953104903625	40.33505740294357	29.69973517699754
5	12.947234140107767	38.80151593713087	35.656224001919995	12.59502592084136
6	29.317417612592635	39.21456334567752	18.653381724981198	12.814637316748648
7	25.973083131837765	33.42763945392592	23.43107127738379	17.168206136852522
8	25.35501162328382	35.42815861394579	21.187912143018327	18.02891761975206
9	26.06178362500439	15.605772931107781	21.199785889241337	37.132657554646485
10-11	24.07670394920432	26.758702719474957	29.295453281086065	19.869140050234655
12-13	24.665464631026623	24.684952562948485	28.56709187893886	22.08249092708603
14-15	22.297246395192342	25.95561138587844	25.97151795332066	25.77562426560856
16-17	21.991108663372295	28.707812186045313	26.75153902510966	22.549540125472735
18-19	21.751230800883047	28.67171113504568	28.0463986915038	21.530659372567474
20-21	23.1719092173762	27.765754628657447	27.23048880574795	21.831847348218407
22-23	23.207965391727758	27.565284228908975	26.77090756945703	22.455842809906237
24-25	22.77171457216595	27.6862970806955	27.271944761680256	22.27004358545829
26-27	22.67567838023769	27.908414442411978	26.882731685563698	22.533175491786636
28-29	22.3473398009566	28.03632973013512	27.116344460824315	22.49998600808397
30-31	22.724278382498607	27.688431877872823	27.03690723027362	22.55038250935495
32-33	22.748219709995414	28.004261770690054	26.631320566827142	22.61619795248739
34-35	22.93077109158524	27.835979393318354	27.036715432589304	22.1965340825071
36-37	22.982075418364314	27.616953655135816	26.951265042335997	22.44970588416387
38-39	22.807482623004187	27.67006360827361	27.210960966423936	22.311492802298265
40-41	22.766588575038433	27.322237060390346	27.376744845241664	22.534429519329553
42-43	22.648167335873577	27.370579413952754	27.133482568433475	22.847770681740194
44-45	22.671884058100822	27.84299952462639	27.01313334352897	22.471983073743814
46-47	22.704749284123164	27.62368748449344	26.84041029572856	22.83115293565483
48-49	22.936521842816365	27.328910487474044	27.08565209919102	22.648915570518565
50-51	22.837354280775138	27.44952729422408	26.842600844645283	22.870517580355504
52-53	22.67703750760353	27.597204521553493	26.859809248151727	22.865948722691257
54-55	22.711622292485213	27.53541598576766	26.884787240963064	22.868174480784063
56-57	22.994766292604353	27.478128316415024	26.734792274151225	22.792313116829398
58-59	22.67497114623674	27.344712561878275	27.186649474586073	22.79366681729891
60-61	22.732227796460172	27.704378129350637	26.896600063194438	22.666794010994757
62-63	22.86320965704027	27.48522507099859	26.694228246831585	22.957337025129558
64-65	22.470342096252903	27.53326318339923	26.858538455457893	23.137856264889972
66-67	22.880720280840734	27.37890378015992	26.733267026327866	23.007108912671477
68-69	22.593198146036052	27.415042888088326	26.949267701259828	23.0424912646158
70-71	22.763691460992806	27.107117615918007	26.88837313043782	23.240817792651367
72-73	22.969133762206493	27.262097662695023	26.962723953658767	22.806044621439717
74-75	22.894490805692936	27.594823464601664	26.781930944444504	22.728754785260893
76-77	22.746327140792292	27.428494138641724	26.758074378345114	23.06710434222087
78-79	22.70012610944795	27.4986449084668	26.810613347850342	22.990615634234903
80-81	22.664763582429547	27.646687865296215	26.905642808242874	22.78290574403136
82-83	23.144485008226905	27.118815057330785	27.264049696200843	22.472650238241464
84-85	23.241929566001826	27.404973712399077	26.7269162931622	22.626180428436896
86-87	22.548941563019685	27.728586817091248	26.898783958249435	22.82368766163964
88-89	23.054739329992795	27.211106066653638	26.8318275534487	22.902327049904862
90-91	22.91769019486484	27.18784610554705	26.9351456705485	22.959318029039615
92-93	23.191342354475882	27.23298694077707	26.848679415934072	22.72699128881297
94-95	22.63526011612471	27.27865854641674	27.09546698005887	22.99061435739968
96-97	22.715373566323123	27.292547841665442	26.96357708616017	23.028501505851263
98-99	22.580236683647815	27.59564869678892	26.830618107321264	22.993496512242
100	22.91180625576448	27.170091597963157	26.56685764596795	23.351244500304418
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1390.0
1	1019.0
2	733.0
3	794.0
4	722.5
5	632.5
6	561.5
7	573.0
8	608.0
9	607.0
10	545.0
11	464.5
12	466.0
13	505.5
14	550.5
15	635.0
16	729.5
17	869.5
18	1139.5
19	1461.5
20	1982.5
21	3031.0
22	4554.0
23	6358.5
24	8926.5
25	12833.0
26	18825.0
27	25682.0
28	32601.0
29	41419.5
30	52526.5
31	63464.5
32	74735.5
33	85821.0
34	95599.5
35	104817.0
36	118932.0
37	134835.5
38	138786.0
39	138352.5
40	138167.0
41	135604.0
42	135523.0
43	140515.5
44	148675.5
45	161429.0
46	173218.0
47	181572.0
48	186852.0
49	184266.0
50	177104.0
51	174814.0
52	173318.0
53	168537.0
54	160202.0
55	143481.5
56	127732.0
57	108195.0
58	92140.0
59	85571.5
60	72544.0
61	56782.5
62	44242.5
63	33316.5
64	23646.0
65	15661.5
66	10895.0
67	8037.5
68	6185.0
69	4953.0
70	3883.0
71	2769.0
72	2109.5
73	1798.0
74	1385.0
75	951.0
76	721.0
77	557.0
78	413.5
79	306.0
80	175.0
81	95.5
82	75.0
83	55.0
84	37.5
85	39.0
86	31.5
87	25.5
88	23.0
89	15.5
90	17.0
91	14.0
92	6.5
93	8.5
94	9.0
95	8.0
96	8.5
97	8.5
98	9.0
99	8.0
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06050833718925678
2	0.009021405803651676
3	0.011036111814392745
4	0.008864706447260706
5	0.010677941856927668
6	0.012133007309129551
7	0.003133987127819441
8	0.005753104941782831
9	0.006850000436519636
10-11	0.007085049471106094
12-13	0.0064022879896882875
14-15	0.009950409130826725
16-17	0.009972794753168293
18-19	0.007745425330182333
20-21	0.0068723860588612035
22-23	0.0028765524708914154
24-25	0.00406299045499449
26-27	0.00505915064919424
28-29	0.006447059234371422
30-31	0.008943056125456192
32-33	0.010409314388828857
34-35	0.012166585742641902
36-37	0.017919690684424734
38-39	0.018613644977013323
40-41	0.018322631886572946
42-43	0.023404168158108755
44-45	0.026683661831148384
46-47	0.029123694666379235
48-49	0.02967214241374764
50-51	0.03073545947497209
52-53	0.029067730610525318
54-55	0.03468652181825874
56-57	0.03751830304446702
58-59	0.03427238780493975
60-61	0.03514542707626087
62-63	0.03123913597765736
64-65	0.03313072106551981
66-67	0.034955149286357554
68-69	0.03792124424661524
70-71	0.03736160368807605
72-73	0.03351127664532645
74-75	0.03621993694865611
76-77	0.03564910357894614
78-79	0.03553717546723831
80-81	0.039622551544574364
82-83	0.03627590100451003
84-85	0.032559887695809836
86-87	0.031194364732974225
88-89	0.02922442996691629
90-91	0.031138400677120308
92-93	0.032649430185176104
94-95	0.036018466347582004
96-97	0.0314853778234146
98-99	0.029515443057356667
100	0.027400001746078543
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4467153.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.88262141434191
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.66860503111229	33.56701422786836
2	14.22531478434285	13.907413542057448
3	6.333624436645335	9.288124965514756
4	3.384370527195309	6.617476128269802
5	2.0291194613912875	4.959433921783185
6	1.3206869844261786	3.873518511993226
7	0.864779458904048	2.959088082755422
8	0.6243764015563468	2.4416924205862416
9	0.47091612214582473	2.071765306508791
>10	1.9879406928290675	15.955097669058901
>50	0.06690271037003609	2.265272543793199
>100	0.0226312989948239	1.8384529952134674
>500	6.405788255747582E-4	0.1806770036898589
>1k	9.151126079634998E-5	0.07497268090717274
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.954248936626975E-5
2	0.0	0.0	0.0	0.0	8.954248936626975E-5
3	0.0	0.0	0.0	0.0	8.954248936626975E-5
4	0.0	0.0	0.0	0.0	8.954248936626975E-5
5	0.0	0.0	0.0	0.0	1.1192811170783718E-4
6	0.0	0.0	0.0	0.0	1.3431373404940462E-4
7	0.0	0.0	0.0	0.0	1.5669935639097207E-4
8	0.0	0.0	0.0	0.0	1.790849787325395E-4
9	0.0	0.0	0.0	4.4771244683134874E-5	1.790849787325395E-4
10-11	0.0	0.0	0.0	4.4771244683134874E-5	1.790849787325395E-4
12-13	0.0	0.0	0.0	4.4771244683134874E-5	2.1266341224489066E-4
14-15	0.0	0.0	0.0	4.4771244683134874E-5	2.6862746809880924E-4
16-17	0.0	0.0	0.0	4.4771244683134874E-5	3.245915239527278E-4
18-19	0.0	0.0	0.0	4.4771244683134874E-5	3.58169957465079E-4
20-21	0.0	0.0	0.0	6.715686702470231E-5	4.589052580021325E-4
22-23	0.0	0.0	0.0	2.462418457572418E-4	4.7009806917291616E-4
24-25	0.0	0.0	0.0	7.275327261009417E-4	4.7009806917291616E-4
26-27	0.0	0.0	0.0	0.0014550654522018833	4.812908803436999E-4
28-29	0.0	0.0	0.0	0.004443546034801136	5.14869313856051E-4
30-31	0.0	0.0	0.0	0.01122638960429607	5.260621250268347E-4
32-33	0.0	0.0	0.0	0.028149920094521053	5.372549361976185E-4
34-35	0.0	0.0	0.0	0.05463211132459533	5.59640558539186E-4
36-37	0.0	0.0	0.0	0.09657157478152192	5.59640558539186E-4
38-39	0.0	0.0	0.0	0.15362133331900654	5.820261808807533E-4
40-41	0.0	0.0	0.0	0.21452141889924076	5.820261808807533E-4
42-43	0.0	0.0	0.0	0.27613784439440514	5.820261808807533E-4
44-45	0.0	0.0	0.0	0.34990966282104063	6.156046143931045E-4
46-47	0.0	0.0	0.0	0.436933769673884	6.491830479054557E-4
48-49	0.0	0.0	0.0	0.532106243059058	7.16339914930158E-4
50-51	0.0	0.0	0.0	0.6272563308218904	7.611111596132928E-4
52-53	0.0	0.0	0.0	0.7222832976618441	8.058824042964278E-4
54-55	0.0	0.0	0.0	0.8256489088240317	8.282680266379951E-4
56-57	0.0	0.0	0.0	0.942333965279452	8.842320824919137E-4
58-59	0.0	0.0	0.0	1.0741628952489426	8.954248936626975E-4
60-61	0.0	0.0	0.0	1.2075812044046845	9.17810516004265E-4
62-63	1.1192811170783718E-5	0.0	0.0	1.341391261951404	0.001029738627712102
64-65	2.2385622341567437E-5	0.0	0.0	1.4796336727217536	0.001029738627712102
66-67	2.2385622341567437E-5	0.0	0.0	1.6328184864050996	0.001029738627712102
68-69	2.2385622341567437E-5	0.0	0.0	1.8064077948527844	0.001029738627712102
70-71	2.2385622341567437E-5	0.0	0.0	1.985683051375227	0.0010521242500536694
72-73	2.2385622341567437E-5	0.0	0.0	2.164902343841816	0.0011416667394199393
74-75	2.2385622341567437E-5	0.0	0.0	2.3477033358830557	0.0012088236064446416
76-77	2.2385622341567437E-5	1.1192811170783718E-5	0.0	2.542469443065863	0.0012088236064446416
78-79	2.2385622341567437E-5	2.2385622341567437E-5	0.0	2.7547187212974347	0.001231209228786209
80-81	2.2385622341567437E-5	2.2385622341567437E-5	0.0	2.9821902227212727	0.0012424020399569927
82-83	2.2385622341567437E-5	2.2385622341567437E-5	0.0	3.214441054515034	0.0012535948511277766
84-85	2.2385622341567437E-5	2.2385622341567437E-5	0.0	3.446255366673136	0.0012535948511277766
86-87	2.2385622341567437E-5	2.2385622341567437E-5	0.0	3.69052727766432	0.0012871732846401276
88	2.2385622341567437E-5	2.2385622341567437E-5	0.0	3.8852262279801026	0.0012983660958109113
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4545	0.0	38.894188	1
GTATCAA	7110	0.0	37.161858	1
CAACGCA	7610	0.0	31.863712	5
ATCAACG	7810	0.0	31.228249	3
TATCAAC	9250	0.0	28.043274	2
AACGCAG	9080	0.0	27.894258	6
TCAACGC	9410	0.0	26.06827	4
GTACTAG	2075	0.0	25.829588	1
ACGCAGA	9615	0.0	25.21725	7
CGCAGAG	9825	0.0	24.584255	8
GTACATG	15155	0.0	24.445618	1
TACATGG	15005	0.0	23.958344	2
ACATGGG	15185	0.0	22.900675	3
GCATCGT	2140	0.0	22.617779	8
GTGATCG	1495	0.0	22.317402	8
TGATCGC	1545	0.0	20.986841	9
CATGGGG	11920	0.0	20.736755	4
TAGGCAT	2980	0.0	20.65791	5
GTATATA	1980	0.0	20.182941	1
GTATAAG	2635	0.0	20.161764	1
>>END_MODULE