FastQCFastQC Report
Mon 16 Jan 2017
ERR1146544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4467153
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147680.33059087074026794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137490.3077799215742107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76960.172279749540703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87550.059.5025521
GTATCAA161250.044.2137151
ATCAACG204050.034.1937983
CAACGCA204900.034.0060585
TATCAAC215300.032.871072
AACGCAG219850.032.1522186
TCAACGC223550.031.316274
ACGCAGA239350.029.1190937
CGCAGAG243000.028.6817078
GTACTAG19200.026.4707741
GTGGTAT26500.025.5717681
GCAGAGT271550.025.5104319
TACATGG148100.024.4014842
GTACATG150000.024.3766421
ACATGGG149900.023.3357283
AGAGTAC251150.023.12892510-11
CAGAGTA258000.023.10685710-11
GAGTACT191200.022.55216812-13
CATGGGG116350.021.4978624
AGTACTT200850.021.28143912-13
Produced by FastQC (version 0.11.5)