FastQCFastQC Report
Mon 16 Jan 2017
ERR1146543_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146543_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5199627
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48550.039.7090871
GTATCAA81050.034.9251941
CAACGCA80250.033.1451645
ATCAACG81550.032.674423
GTACTAG24600.030.3918651
AACGCAG95500.028.9824896
TATCAAC97700.028.4276982
GCATCGT25250.026.9849728
TCAACGC103550.026.004794
ACGCAGA103550.025.7746477
CGCAGAG105250.025.3595568
GTACATG166600.025.3170281
TACATGG166500.024.7533632
ACATGGG167350.024.2064083
CATGGGG137550.022.5492524
TAGGCAT35050.022.3911935
GTATAAG26250.021.3163571
CTAGTAC36450.021.0154573
TAGTACT37300.020.9145264
CGACGAG15950.020.7698524-25
Produced by FastQC (version 0.11.5)