##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146543_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5199627
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.346363114123378	31.0	31.0	34.0	30.0	34.0
2	31.54764178276634	31.0	31.0	34.0	30.0	34.0
3	31.64421082512265	33.0	31.0	34.0	30.0	34.0
4	35.23296671088138	37.0	35.0	37.0	33.0	37.0
5	35.262953861882785	37.0	35.0	37.0	33.0	37.0
6	35.445456183683945	37.0	35.0	37.0	33.0	37.0
7	35.42076960520437	37.0	35.0	37.0	33.0	37.0
8	35.34595462328355	37.0	35.0	37.0	33.0	37.0
9	37.03776713214236	39.0	38.0	39.0	34.0	39.0
10-11	36.99959612487588	39.0	38.0	39.0	34.0	39.0
12-13	36.95521919168432	39.0	38.0	39.0	33.0	39.0
14-15	38.395752906891204	41.0	39.0	41.0	34.0	41.0
16-17	38.40569438923215	41.0	39.0	41.0	34.0	41.0
18-19	38.43670959474593	41.0	39.0	41.0	34.0	41.0
20-21	38.52183810492561	41.0	39.0	41.0	34.0	41.0
22-23	38.54200628237372	41.0	39.0	41.0	34.0	41.0
24-25	38.59465400114277	41.0	39.0	41.0	34.0	41.0
26-27	38.62138178373179	41.0	39.0	41.0	34.0	41.0
28-29	38.589473437229245	41.0	39.0	41.0	34.0	41.0
30-31	38.57726275365522	41.0	39.0	41.0	34.0	41.0
32-33	38.59101883654347	41.0	39.0	41.0	34.0	41.0
34-35	38.53811504171357	40.5	38.5	41.0	33.5	41.0
36-37	38.45065472965657	40.0	38.0	41.0	33.0	41.0
38-39	38.41273220175216	40.0	38.0	41.0	33.0	41.0
40-41	38.349744318198205	40.0	38.0	41.0	33.0	41.0
42-43	38.232015007999614	40.0	38.0	41.0	33.0	41.0
44-45	38.10572450677712	40.0	38.0	41.0	33.0	41.0
46-47	38.00695347954766	40.0	38.0	41.0	33.0	41.0
48-49	37.890308189414355	40.0	37.0	41.0	33.0	41.0
50-51	37.76439444598623	40.0	37.0	41.0	32.0	41.0
52-53	37.6377118974111	40.0	37.0	41.0	32.0	41.0
54-55	37.458164691428834	40.0	36.0	41.0	32.0	41.0
56-57	37.32231263896429	39.5	36.0	41.0	32.0	41.0
58-59	37.1010688843642	39.0	35.0	41.0	31.0	41.0
60-61	36.91842789877043	39.0	35.0	41.0	31.0	41.0
62-63	36.67801026881351	38.5	35.0	41.0	31.0	41.0
64-65	36.42521377014159	38.0	35.0	40.0	31.0	41.0
66-67	36.16148860293248	37.0	35.0	40.0	31.0	41.0
68-69	35.869449577825485	37.0	35.0	40.0	30.0	41.0
70-71	35.5979908751147	36.0	35.0	39.0	30.0	41.0
72-73	35.28291885167917	36.0	35.0	39.0	30.0	41.0
74-75	34.964147332106705	35.0	34.5	38.5	30.0	40.5
76-77	34.65329301505666	35.0	34.0	37.0	30.0	39.5
78-79	34.374448013290184	35.0	34.0	37.0	29.0	39.0
80-81	34.09864072942155	35.0	34.0	36.5	29.0	39.0
82-83	33.84816093539017	35.0	34.0	36.0	29.0	37.5
84-85	33.62933831599844	35.0	34.0	36.0	29.0	37.0
86-87	33.436125899030834	35.0	34.0	35.0	29.0	37.0
88-89	33.298956540536466	35.0	34.0	35.0	29.0	36.0
90-91	33.14312267014538	35.0	34.0	35.0	28.5	36.0
92-93	33.051112800975915	35.0	34.0	35.0	29.0	36.0
94-95	32.96042898846398	35.0	34.0	35.0	28.0	36.0
96-97	32.88572507220229	35.0	34.0	35.0	28.0	35.5
98-99	32.82073252562155	35.0	34.0	35.0	28.0	35.0
100	31.94869343512525	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.212552067060102
1101	2	0.21206711359906905
1101	3	0.252525304125669
1101	4	0.3305495107415126
1101	5	0.25534183159076207
1101	6	0.3321229711327689
1101	7	0.35031158930227235
1101	8	0.38831981027747986
1101	9	0.4948918581843387
1101	10-11	0.5191179981735203
1101	12-13	0.493233847423177
1101	14-15	0.5592310802575469
1101	16-17	0.5786120979764533
1101	18-19	0.5007879722521054
1101	20-21	0.4882627335285932
1101	22-23	0.4998242827757906
1101	24-25	0.5137341785631904
1101	26-27	0.4696383973974321
1101	28-29	0.5011469528977344
1101	30-31	0.4567385910783628
1101	32-33	0.5009303549410049
1101	34-35	0.46691881765448073
1101	36-37	0.48415174692149776
1101	38-39	0.4480072745707986
1101	40-41	0.45534886815112685
1101	42-43	0.41302524718245337
1101	44-45	0.35577016126394767
1101	46-47	0.3494621542701779
1101	48-49	0.37184940804710465
1101	50-51	0.3667881406660456
1101	52-53	0.3744101172889458
1101	54-55	0.4756313603671458
1101	56-57	0.38708290526351163
1101	58-59	0.39436418127048256
1101	60-61	0.41016291022177853
1101	62-63	0.3870298674255821
1101	64-65	0.35534128994380154
1101	66-67	0.35082397206004146
1101	68-69	0.34045909076963454
1101	70-71	0.3061045745765085
1101	72-73	0.3393125391830125
1101	74-75	0.3614590258449155
1101	76-77	0.2793116831846376
1101	78-79	0.2939850697717219
1101	80-81	0.29583431466862464
1101	82-83	0.2676474044069934
1101	84-85	0.26513138541736936
1101	86-87	0.2849386021989986
1101	88-89	0.25379694858027335
1101	90-91	0.2991502538363804
1101	92-93	0.3121589261913513
1101	94-95	0.2839096570204589
1101	96-97	0.26350753284744854
1101	98-99	0.23361876975994278
1101	100	0.15543088422860407
1102	1	0.15864790380468818
1102	2	0.17477089199292806
1102	3	0.2524676510387067
1102	4	0.24470886867830188
1102	5	0.22358966589469986
1102	6	0.24528885578879311
1102	7	0.23837490290709695
1102	8	0.2873140494559294
1102	9	0.37633206946295417
1102	10-11	0.3837352811639292
1102	12-13	0.4117522455792866
1102	14-15	0.4342030978876039
1102	16-17	0.4382079534628289
1102	18-19	0.39248318104942115
1102	20-21	0.40916088522591565
1102	22-23	0.42791832837675514
1102	24-25	0.4710993128316616
1102	26-27	0.5013358674467199
1102	28-29	0.5389774966964254
1102	30-31	0.5302416764929987
1102	32-33	0.49439418549278
1102	34-35	0.4793923712271351
1102	36-37	0.5364165591530536
1102	38-39	0.5257840536931724
1102	40-41	0.5047324197707397
1102	42-43	0.49281611106274426
1102	44-45	0.4107930187639681
1102	46-47	0.43510001338444226
1102	48-49	0.44732897000511684
1102	50-51	0.4186841340439216
1102	52-53	0.3469532312146626
1102	54-55	0.3475230737285102
1102	56-57	0.35996986410351894
1102	58-59	0.38032931456900343
1102	60-61	0.39116639936666786
1102	62-63	0.35902608153953963
1102	64-65	0.29767842416567447
1102	66-67	0.33792957814740987
1102	68-69	0.36184219920380656
1102	70-71	0.3569465408024044
1102	72-73	0.3491029806365944
1102	74-75	0.33700562991265315
1102	76-77	0.30913832869189406
1102	78-79	0.3236446708984744
1102	80-81	0.32230068490233066
1102	82-83	0.31576696195218545
1102	84-85	0.3054035397151651
1102	86-87	0.27495855596865226
1102	88-89	0.2684730472375705
1102	90-91	0.32570990241361386
1102	92-93	0.2798728122319645
1102	94-95	0.297560509744514
1102	96-97	0.23594136879151506
1102	98-99	0.22838749997654162
1102	100	0.20521722560495093
1103	1	-0.029506380573533164
1103	2	-0.015739523870994532
1103	3	0.022014204343701493
1103	4	0.053094992987155365
1103	5	0.06051109488926443
1103	6	0.10560266327630785
1103	7	0.1326771603372805
1103	8	0.17525137707924188
1103	9	0.2562085506377585
1103	10-11	0.265656200335016
1103	12-13	0.239912738270597
1103	14-15	0.2657937261526371
1103	16-17	0.26232072300741294
1103	18-19	0.20489481383183517
1103	20-21	0.2727144308450278
1103	22-23	0.2623171141318963
1103	24-25	0.2092693768361258
1103	26-27	0.19236233213417364
1103	28-29	0.2238447630413205
1103	30-31	0.24970482320210863
1103	32-33	0.21693108281983342
1103	34-35	0.20623911173826315
1103	36-37	0.22155643033678274
1103	38-39	0.18353929439012973
1103	40-41	0.16672355303623476
1103	42-43	0.163394624401775
1103	44-45	0.16940048378535977
1103	46-47	0.150971984521874
1103	48-49	0.12014425191784284
1103	50-51	0.14524136297379187
1103	52-53	0.1776762421560747
1103	54-55	0.12212539831384817
1103	56-57	0.07769200952456856
1103	58-59	0.174330848233339
1103	60-61	0.07768030378616686
1103	62-63	0.12743867079796445
1103	64-65	0.09030081325351347
1103	66-67	0.1316671896991437
1103	68-69	0.06245632679230795
1103	70-71	0.06553999550779821
1103	72-73	0.08567286734307089
1103	74-75	0.1318092508912727
1103	76-77	0.11181321780201614
1103	78-79	0.15023860318657967
1103	80-81	0.1291169723075143
1103	82-83	0.03378536854544478
1103	84-85	0.06399983186996394
1103	86-87	0.07818271761703244
1103	88-89	0.019925356069499855
1103	90-91	0.11506637413112486
1103	92-93	0.08226382534163434
1103	94-95	-0.02379632972740353
1103	96-97	0.03680104387800043
1103	98-99	0.061152327713870136
1103	100	0.03052844072528771
1104	1	0.0358890278566264
1104	2	0.10424208290285719
1104	3	0.11695609267068718
1104	4	0.17119625729179688
1104	5	0.1888902888025612
1104	6	0.2474549974144722
1104	7	0.22414090950348964
1104	8	0.25885449729305776
1104	9	0.3947503337293625
1104	10-11	0.3975978378220901
1104	12-13	0.34973721029466986
1104	14-15	0.43232170360715827
1104	16-17	0.3498900420956943
1104	18-19	0.3481325090238201
1104	20-21	0.37403168490580185
1104	22-23	0.32112689020303975
1104	24-25	0.3157517514404091
1104	26-27	0.34046014643301703
1104	28-29	0.3680406195129038
1104	30-31	0.3653213560789794
1104	32-33	0.31834607003286663
1104	34-35	0.31989249690334276
1104	36-37	0.32109308460690045
1104	38-39	0.31716131053065055
1104	40-41	0.30475137585292345
1104	42-43	0.264699342846086
1104	44-45	0.2533228635034064
1104	46-47	0.24956470368272932
1104	48-49	0.2972276011997934
1104	50-51	0.2940777898293305
1104	52-53	0.22022882284716871
1104	54-55	0.23484859608134911
1104	56-57	0.18033733719090606
1104	58-59	0.21779246844996436
1104	60-61	0.20311522269316384
1104	62-63	0.18050467844207674
1104	64-65	0.15312520821385078
1104	66-67	0.08932921365831703
1104	68-69	0.13942147127150406
1104	70-71	0.08604791316901839
1104	72-73	0.1251565504998453
1104	74-75	0.0914058745298334
1104	76-77	0.11729727887732366
1104	78-79	0.11033907567406231
1104	80-81	0.12390273000993801
1104	82-83	0.134587864900233
1104	84-85	0.12613763867678784
1104	86-87	0.1210550681682463
1104	88-89	0.15148531114797237
1104	90-91	0.17897192613970958
1104	92-93	0.17155929562692762
1104	94-95	0.11539593383874802
1104	96-97	0.14992461583843664
1104	98-99	0.13912006633204754
1104	100	0.18452048274595612
1105	1	0.05961867960214917
1105	2	0.17390255993253945
1105	3	0.17556159744537325
1105	4	0.23159190672981822
1105	5	0.14672238511440128
1105	6	0.24385885479382807
1105	7	0.2058471373974342
1105	8	0.2788488110126792
1105	9	0.2982167813069907
1105	10-11	0.3413795023087829
1105	12-13	0.3056780608999716
1105	14-15	0.3615493183495815
1105	16-17	0.394972658081997
1105	18-19	0.3767046742632303
1105	20-21	0.35185252015256907
1105	22-23	0.36332139025983423
1105	24-25	0.3531781846072235
1105	26-27	0.3351129407125413
1105	28-29	0.36396847157856627
1105	30-31	0.3191363554552993
1105	32-33	0.3326691436718221
1105	34-35	0.33085223325318935
1105	36-37	0.324036716100629
1105	38-39	0.2903996146904433
1105	40-41	0.2891945907476341
1105	42-43	0.3226958604639165
1105	44-45	0.2770566092314368
1105	46-47	0.23623118336182358
1105	48-49	0.24148425476339241
1105	50-51	0.2159209925059713
1105	52-53	0.22832613954069814
1105	54-55	0.2797725339513022
1105	56-57	0.20386896793853992
1105	58-59	0.21493031011167574
1105	60-61	0.19604648281053016
1105	62-63	0.20796603879639264
1105	64-65	0.21545977938166772
1105	66-67	0.18989873598521
1105	68-69	0.23140443147753587
1105	70-71	0.20412057537725303
1105	72-73	0.2384335912833464
1105	74-75	0.24583133025106463
1105	76-77	0.23093609995901687
1105	78-79	0.20397098169991068
1105	80-81	0.1878036124286666
1105	82-83	0.20275235426505134
1105	84-85	0.23453357800361374
1105	86-87	0.24701120181349268
1105	88-89	0.261809312988575
1105	90-91	0.21632791957910769
1105	92-93	0.21783294113426876
1105	94-95	0.2368439249302412
1105	96-97	0.17956748816262547
1105	98-99	0.18379244204280099
1105	100	0.13927564633220513
1106	1	0.004845166268179213
1106	2	0.03846998431176374
1106	3	0.05924057182183873
1106	4	0.11872689073943121
1106	5	0.11787657741210467
1106	6	0.18803965906892728
1106	7	0.19567444294510494
1106	8	0.17753731199696432
1106	9	0.2552228715442908
1106	10-11	0.2168661259253568
1106	12-13	0.19091307946566616
1106	14-15	0.24296343699661804
1106	16-17	0.17854866741198805
1106	18-19	0.20398278548078252
1106	20-21	0.18801479618473138
1106	22-23	0.21108265563936612
1106	24-25	0.21258362021724508
1106	26-27	0.23837207100386593
1106	28-29	0.19887938289300422
1106	30-31	0.21401319309663336
1106	32-33	0.22814360780104437
1106	34-35	0.18657200188625467
1106	36-37	0.1759677215399691
1106	38-39	0.17993465093498173
1106	40-41	0.1423812421577324
1106	42-43	0.11063093232848331
1106	44-45	0.08405362321229859
1106	46-47	0.07740666095655513
1106	48-49	0.06411553036983975
1106	50-51	0.14870298789490022
1106	52-53	0.09435443676503041
1106	54-55	0.13841229933717614
1106	56-57	0.09575013084783279
1106	58-59	0.14821623631031144
1106	60-61	0.10943095871738251
1106	62-63	0.08561651700689055
1106	64-65	0.09419239439726113
1106	66-67	0.025670962538484332
1106	68-69	0.0018616795041168643
1106	70-71	0.03377713216547562
1106	72-73	0.0507174244411317
1106	74-75	0.016161434171472422
1106	76-77	0.07142905472901617
1106	78-79	0.01591471940361089
1106	80-81	0.05080105577366112
1106	82-83	0.09745799643916087
1106	84-85	0.1298331483917181
1106	86-87	0.042523089559686866
1106	88-89	0.004155671387536586
1106	90-91	0.08319621686757728
1106	92-93	0.07524416907925513
1106	94-95	0.11589337088872753
1106	96-97	0.04607703466461288
1106	98-99	-0.016095472930857113
1106	100	-0.025695935621037336
1107	1	0.012963211500853333
1107	2	0.009013201955056616
1107	3	0.006689563530521525
1107	4	0.05483645224042988
1107	5	0.018361639035965993
1107	6	0.028925100095506195
1107	7	0.018178384555476157
1107	8	0.007010379396199085
1107	9	0.04035506392298771
1107	10-11	0.1166144832443905
1107	12-13	0.060636010792705974
1107	14-15	0.08365234276293165
1107	16-17	0.06429419035814732
1107	18-19	0.03912871836411114
1107	20-21	0.10917713624841241
1107	22-23	0.12169509925362121
1107	24-25	0.1273369963343569
1107	26-27	0.12764153659759359
1107	28-29	0.1468028703348665
1107	30-31	0.13442029007939027
1107	32-33	0.15834160192970614
1107	34-35	0.09094790428844846
1107	36-37	0.14136140032623246
1107	38-39	0.10832881003305772
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2216	30-31	-0.4573431972016806
2216	32-33	-0.4493562302532723
2216	34-35	-0.4706933033874705
2216	36-37	-0.4247142456536679
2216	38-39	-0.42159159653792244
2216	40-41	-0.412368201168654
2216	42-43	-0.369063672256452
2216	44-45	-0.3633216529487271
2216	46-47	-0.3017847290262097
2216	48-49	-0.3457807993808544
2216	50-51	-0.34973850317688004
2216	52-53	-0.2770408908506852
2216	54-55	-0.30068497297644825
2216	56-57	-0.2794739287506758
2216	58-59	-0.28530799402037843
2216	60-61	-0.30357506680837787
2216	62-63	-0.31142498689546017
2216	64-65	-0.3056569192527263
2216	66-67	-0.36249359907756684
2216	68-69	-0.31611453055842276
2216	70-71	-0.3414067080999601
2216	72-73	-0.2780244340427913
2216	74-75	-0.29254517721982864
2216	76-77	-0.25210716870191874
2216	78-79	-0.2993520730082935
2216	80-81	-0.2698227251063017
2216	82-83	-0.2494486951771293
2216	84-85	-0.21882177483769283
2216	86-87	-0.1871441809919503
2216	88-89	-0.2932826215762816
2216	90-91	-0.2614723542003645
2216	92-93	-0.2497401588003143
2216	94-95	-0.3065286297926093
2216	96-97	-0.27815750947693374
2216	98-99	-0.312350337750388
2216	100	-0.31164736817713745
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	125.0
1	73.0
2	65.0
3	73.0
4	47.0
5	78.0
6	174.0
7	953.0
8	2733.0
9	3606.0
10	3436.0
11	4016.0
12	5007.0
13	6279.0
14	7518.0
15	9016.0
16	9862.0
17	10785.0
18	12199.0
19	13968.0
20	16116.0
21	17735.0
22	20396.0
23	24100.0
24	29872.0
25	37848.0
26	42416.0
27	40742.0
28	43452.0
29	55639.0
30	62755.0
31	69777.0
32	88225.0
33	120234.0
34	181493.0
35	285047.0
36	547195.0
37	1255805.0
38	1612350.0
39	557227.0
40	1190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.445188675196775	20.114725203924166	13.12706428294727	24.31302183793179
2	15.165045892028711	20.87616076443118	40.11222966786943	23.846563675670684
3	17.854118264366527	26.2808549336061	30.557273284892407	25.30775351713497
4	12.217713798162624	17.74018706113069	39.69727009537702	30.344829045329664
5	13.160019118932647	38.479448589978716	35.628034817999875	12.732497473088756
6	29.923731545503717	38.70033567968019	18.47096097637163	12.90497179844447
7	26.175278729259986	32.95146254728829	23.51028658579976	17.362972137651962
8	25.343205570221883	35.28594469859956	21.16021157980537	18.21063815137319
9	26.225290695437963	15.447633276761136	20.87602533901004	37.45105068879086
10-11	24.312256396793018	26.44121258980802	29.297057191517677	19.949473821881277
12-13	24.89285401402084	24.42546700474859	28.454292633581836	22.227386347648732
14-15	22.416844845807123	25.726904054848497	25.82399738109602	26.03225371824836
16-17	22.18783412907223	28.47663012672646	26.717756418307527	22.617779325893785
18-19	22.059609420897736	28.454625527614336	27.85022816365928	21.635536887828646
20-21	23.34298338001925	27.564795460297997	27.143535532969537	21.948685626713214
22-23	23.32527872354052	27.330177649534846	26.768345537374987	22.576198089549642
24-25	22.945168858114716	27.492078975984786	27.10594227778532	22.456809888115174
26-27	22.809926797055265	27.718087980088402	26.801594266702132	22.670390956154204
28-29	22.55018746159963	27.92022397494755	27.011337899881937	22.518250663570885
30-31	22.90252156812473	27.496970427273375	26.979339280505688	22.6211687240962
32-33	22.933034281603135	27.80475199508069	26.4688670665243	22.793346656791883
34-35	23.094375296930387	27.752859668053464	26.864578584851408	22.288186450164744
36-37	23.113223470917667	27.450305262964527	26.789276464402885	22.647194801714924
38-39	22.97299428816326	27.43852379393377	27.04536614885554	22.54311576904744
40-41	22.917966269663797	27.179779264174158	27.291739882043657	22.610514584118384
42-43	22.83193586472728	27.23583995998759	26.960773893827657	22.97145028145747
44-45	22.792702923126452	27.635044583062445	27.050719395500593	22.52153309831051
46-47	22.935103315773688	27.49292865672084	26.715874456266942	22.856093571238528
48-49	23.06125958945577	27.185237783489775	26.913952159155773	22.839550467898686
50-51	22.931791324807268	27.313222004883624	26.778120164890893	22.976866505418208
52-53	22.794167297830292	27.497575542927365	26.771046413179043	22.9372107460633
54-55	22.751962525446025	27.373899214797433	26.813745210202768	23.060393049553777
56-57	23.031792988488586	27.310257533057413	26.72645231744351	22.93149716101049
58-59	22.801487676302752	27.25518566085209	27.054070586610163	22.889256076234993
60-61	22.820052240077334	27.493185250840586	26.83506185917381	22.85170064990827
62-63	22.957277904472633	27.255555346071908	26.706619473848043	23.080547275607422
64-65	22.633204312312312	27.356454895581084	26.753495332022155	23.256845460084445
66-67	22.941898053110148	27.203540548309014	26.70484169423321	23.149719704347632
68-69	22.69972986578521	27.19028190214275	26.951556405391987	23.158431826680058
70-71	22.803029042254273	27.098671092798014	26.783363104992453	23.31493675995526
72-73	22.948932055141295	27.217075343550906	26.871824203671913	22.96216839763589
74-75	22.97272208160404	27.520805420260253	26.687588164267005	22.8188843338687
76-77	22.89841485106865	27.28532459513207	26.644071474889873	23.17218907890941
78-79	22.924879589228254	27.289122546512385	26.732850705841987	23.053147158417374
80-81	22.85136149398296	27.5080716861546	26.729041658385423	22.911525161477016
82-83	23.175524068201614	27.021318656265226	27.225236656179614	22.57792061935355
84-85	23.226221571785228	27.455211006502083	26.557064783999145	22.76150263771354
86-87	22.730768427936933	27.515862763802645	26.82291563961786	22.930453168642558
88-89	23.17493449479229	27.06460679561221	26.73851398406328	23.02194472553222
90-91	23.03788908480002	27.160680154715287	26.763974539889396	23.037456220595296
92-93	23.265288768395042	27.133499734891302	26.862061522147158	22.739149974566494
94-95	22.77626919989749	27.049210763870985	27.113316238540087	23.061203797691434
96-97	22.940815071519246	27.0594590757313	26.95418653152073	23.045539321228723
98-99	22.76375013358376	27.3271637755662	26.674005316802003	23.235080774048033
100	23.027179201279836	27.015912920056806	26.47968533517398	23.477222543489376
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1426.0
1	1066.0
2	782.5
3	849.5
4	788.0
5	708.5
6	672.5
7	680.5
8	692.5
9	692.0
10	643.5
11	558.5
12	538.5
13	576.0
14	687.5
15	785.5
16	886.0
17	1164.5
18	1488.5
19	2000.0
20	2668.0
21	3795.0
22	6098.5
23	9001.0
24	12278.0
25	16692.5
26	22515.0
27	29815.0
28	38129.5
29	47707.0
30	58932.0
31	69883.0
32	84580.0
33	98233.0
34	107207.0
35	115730.0
36	125495.5
37	140265.5
38	148339.5
39	148348.5
40	149298.0
41	152928.0
42	157607.5
43	166786.5
44	178417.5
45	194010.5
46	206176.0
47	211331.0
48	213568.5
49	207599.5
50	201473.0
51	205874.0
52	207029.5
53	199505.0
54	193203.5
55	178498.5
56	161427.0
57	139508.0
58	117488.0
59	104668.5
60	87100.0
61	68761.0
62	54545.0
63	41221.5
64	29134.5
65	19208.0
66	13419.0
67	9870.0
68	7338.5
69	5314.0
70	4048.0
71	2872.0
72	1961.5
73	1530.0
74	1217.5
75	951.5
76	666.5
77	419.5
78	262.5
79	161.5
80	89.5
81	73.5
82	65.0
83	47.0
84	33.0
85	28.0
86	24.5
87	21.0
88	21.0
89	17.5
90	11.5
91	9.5
92	13.0
93	14.5
94	10.5
95	11.5
96	12.0
97	8.5
98	6.0
99	6.0
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.061735197543977674
2	0.008981413474466534
3	0.011154646285204689
4	0.009058342069536911
5	0.01138543207041582
6	0.011981628682211243
7	0.0026348043811604176
8	0.005096519423412487
9	0.007135127192777481
10-11	0.006577394878517248
12-13	0.005683099960824113
14-15	0.009721851202018914
16-17	0.009154502813374881
18-19	0.007664011283886325
20-21	0.006596627027284841
22-23	0.0023078578521113146
24-25	0.0033271617367938125
26-27	0.004731108596828196
28-29	0.005683099960824113
30-31	0.008875636656244764
32-33	0.010125726326138396
34-35	0.011481592814253792
36-37	0.01754933575042979
38-39	0.01825130918044698
40-41	0.017770505461257125
42-43	0.023838248397433124
44-45	0.027088481539156557
46-47	0.029502116209489643
48-49	0.030771438028150865
50-51	0.031627268648308816
52-53	0.029925223482376716
54-55	0.03577179670772538
56-57	0.038521993981491365
58-59	0.0357044841870388
60-61	0.036416073691439795
62-63	0.03194459910297412
64-65	0.033963974723571516
66-67	0.03656031480719675
68-69	0.03895471732876224
70-71	0.03866623509724832
72-73	0.03493519823633503
74-75	0.037368065055435704
76-77	0.036800716666791675
78-79	0.03651223443527776
80-81	0.04111833406511659
82-83	0.03749307402242507
84-85	0.03373318893836039
86-87	0.032377322450244994
88-89	0.030136777118820252
90-91	0.03254079571476954
92-93	0.03382934968219836
94-95	0.037368065055435704
96-97	0.03251194749161815
98-99	0.030338714680879994
100	0.028098169349455263
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5199627.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.74071103286719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.18531344080519	34.59771285985372
2	14.74543926214781	14.963881453066733
3	6.53963733482648	9.954775447985405
4	3.4291580228541507	6.959916652947226
5	2.0178244115667274	5.119292269118631
6	1.2781159637460902	3.891150766976096
7	0.8768377779314632	3.114396061890523
8	0.6124843222266785	2.486231200501233
9	0.461619777492309	2.108062416311433
>10	1.8017986062189766	14.556889740204443
>50	0.0409522007049482	1.3739908973457386
>100	0.01074135799052427	0.8539147516461452
>500	7.752148871703978E-5	0.019785482152879823
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.7696446302782875E-5
2	0.0	0.0	0.0	0.0	5.7696446302782875E-5
3	0.0	0.0	0.0	0.0	5.7696446302782875E-5
4	0.0	0.0	0.0	0.0	5.7696446302782875E-5
5	0.0	0.0	0.0	0.0	5.7696446302782875E-5
6	0.0	0.0	0.0	0.0	5.7696446302782875E-5
7	0.0	0.0	0.0	0.0	5.7696446302782875E-5
8	0.0	0.0	0.0	0.0	7.692859507037716E-5
9	0.0	0.0	0.0	0.0	7.692859507037716E-5
10-11	0.0	0.0	0.0	1.923214876759429E-5	9.616074383797146E-5
12-13	0.0	0.0	0.0	1.923214876759429E-5	4.038751241194801E-4
14-15	0.0	0.0	0.0	1.923214876759429E-5	5.673483886440316E-4
16-17	0.0	0.0	0.0	4.808037191898573E-5	6.731252068658002E-4
18-19	0.0	0.0	0.0	5.7696446302782875E-5	7.30821653168583E-4
20-21	0.0	0.0	0.0	6.731252068658002E-5	8.173663226227574E-4
22-23	0.0	0.0	0.0	2.2116971082733434E-4	8.750627689255402E-4
24-25	0.0	0.0	0.0	6.154287605630173E-4	9.23143140844526E-4
26-27	1.923214876759429E-5	0.0	0.0	0.001182777149207049	0.0010193038846824974
28-29	1.923214876759429E-5	0.0	0.0	0.004182992356951758	0.0010770003309852802
30-31	1.923214876759429E-5	0.0	0.0	0.012875923599904379	0.0010962324797528745
32-33	1.923214876759429E-5	0.0	0.0	0.03212730451626626	0.0011346967772880633
34-35	1.923214876759429E-5	0.0	0.0	0.06317760870154725	0.001192393223590846
36-37	1.923214876759429E-5	0.0	0.0	0.1115272307032793	0.001192393223590846
38-39	1.923214876759429E-5	0.0	0.0	0.1769069204387161	0.0012212414467422375
40-41	1.923214876759429E-5	0.0	0.0	0.2431905211662298	0.0012308575211260346
42-43	1.923214876759429E-5	0.0	0.0	0.3118589083409252	0.0012500896698936289
44-45	1.923214876759429E-5	0.0	0.0	0.39678807729862164	0.0013077861161964119
46-47	1.923214876759429E-5	0.0	0.0	0.4979491798161676	0.0013654825624991947
48-49	3.846429753518858E-5	0.0	0.0	0.6021585779133772	0.0014231790088019775
50-51	3.846429753518858E-5	0.0	0.0	0.7074065120440369	0.0014808754551047605
52-53	3.846429753518858E-5	1.923214876759429E-5	0.0	0.8101446507605257	0.0015578040501751376
54-55	3.846429753518858E-5	1.923214876759429E-5	0.0	0.9214410956785939	0.0016347326452455149
56-57	3.846429753518858E-5	1.923214876759429E-5	0.0	1.0512388677110878	0.0017020451659320948
58-59	3.846429753518858E-5	1.923214876759429E-5	0.0	1.1918547234253536	0.0017597416122348776
60-61	5.7696446302782875E-5	1.923214876759429E-5	0.0	1.3346534280247409	0.001788589835386269
62-63	5.7696446302782875E-5	1.923214876759429E-5	0.0	1.4766155341527383	0.0018847505792242405
64-65	5.7696446302782875E-5	1.923214876759429E-5	0.0	1.6266936070606604	0.0018943666536080377
66-67	5.7696446302782875E-5	1.923214876759429E-5	0.0	1.7914938898501758	0.001913598802375632
68-69	5.7696446302782875E-5	1.923214876759429E-5	0.0	1.9707855967360737	0.001923214876759429
70-71	5.7696446302782875E-5	1.923214876759429E-5	0.0	2.160568440774694	0.0019712952486784147
72-73	5.7696446302782875E-5	1.923214876759429E-5	0.0	2.352966857045707	0.0021059202900515746
74-75	5.7696446302782875E-5	1.923214876759429E-5	0.0	2.545192183977812	0.00222131318265714
76-77	5.7696446302782875E-5	1.923214876759429E-5	0.0	2.753293649717566	0.002250161405808532
78-79	5.7696446302782875E-5	1.923214876759429E-5	0.0	2.981261925134245	0.0022790096289599234
80-81	5.7696446302782875E-5	1.923214876759429E-5	0.0	3.2214810793158817	0.0023463221496465036
82-83	5.7696446302782875E-5	1.923214876759429E-5	0.0	3.4662005563091354	0.0025001793397872577
84-85	5.7696446302782875E-5	1.923214876759429E-5	0.0	3.715554981155379	0.0025001793397872577
86-87	5.7696446302782875E-5	1.923214876759429E-5	0.0	3.9742466142282895	0.002596340083625229
88	5.7696446302782875E-5	1.923214876759429E-5	0.0	4.175299497444721	0.002692500827463201
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4855	0.0	39.709087	1
GTATCAA	8105	0.0	34.925194	1
CAACGCA	8025	0.0	33.145164	5
ATCAACG	8155	0.0	32.67442	3
GTACTAG	2460	0.0	30.391865	1
AACGCAG	9550	0.0	28.982489	6
TATCAAC	9770	0.0	28.427698	2
GCATCGT	2525	0.0	26.984972	8
TCAACGC	10355	0.0	26.00479	4
ACGCAGA	10355	0.0	25.774647	7
CGCAGAG	10525	0.0	25.359556	8
GTACATG	16660	0.0	25.317028	1
TACATGG	16650	0.0	24.753363	2
ACATGGG	16735	0.0	24.206408	3
CATGGGG	13755	0.0	22.549252	4
TAGGCAT	3505	0.0	22.391193	5
GTATAAG	2625	0.0	21.316357	1
CTAGTAC	3645	0.0	21.015457	3
TAGTACT	3730	0.0	20.914526	4
CGACGAG	1595	0.0	20.76985	24-25
>>END_MODULE