FastQCFastQC Report
Mon 16 Jan 2017
ERR1146543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5199627
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161770.31111847061337283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147410.28350110498310743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83210.1600307098951521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93650.060.6075171
GTATCAA180600.043.597861
ATCAACG221150.034.848323
CAACGCA223050.034.509315
TATCAAC234650.033.393012
TCAACGC239300.032.2838064
AACGCAG243250.032.1319476
ACGCAGA264750.029.0590347
CGCAGAG266800.028.8707078
GTGGTAT25000.026.167471
GCAGAGT296250.025.794469
GTACTAG21500.025.6114641
TACATGG161500.022.816612
GTACATG165600.022.7361351
AGAGTAC277750.022.5548710-11
CAGAGTA291150.022.51757810-11
GAGTACT214850.022.42088712-13
ACATGGG161550.022.1787913
CTAGTAC35250.022.0097273
TAGTACT37450.021.595664
Produced by FastQC (version 0.11.5)