FastQCFastQC Report
Mon 16 Jan 2017
ERR1146542_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146542_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4419174
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41300.035.8687061
GTATCAA64650.030.2607731
CAACGCA62250.029.7462235
ATCAACG64600.028.8102783
AACGCAG74450.026.1333966
TCAACGC73150.025.6998424
TATCAAC78000.025.2466752
ACGCAGA77400.024.0417067
GTACATG126900.024.0142271
CGCAGAG79650.023.4234148
TACATGG127900.022.8929792
ACATGGG124900.022.5397553
GTACTAG18800.022.0130391
TATTCGC3301.8189894E-1221.3610619
GCTATTG21450.019.9369898
CATGGGG98650.019.342554
TGATCGC11550.018.7163589
GTATAAG24450.018.4649261
GCAGAGT98200.018.280899
GAGTACA93050.017.8913081
Produced by FastQC (version 0.11.5)