##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146542_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4419174 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.618741647194703 33.0 31.0 34.0 30.0 34.0 2 31.695348044679843 33.0 31.0 34.0 30.0 34.0 3 31.87907875996736 34.0 31.0 34.0 30.0 34.0 4 35.38336643906757 37.0 35.0 37.0 33.0 37.0 5 35.36732543230929 37.0 35.0 37.0 33.0 37.0 6 35.45023685421755 37.0 35.0 37.0 33.0 37.0 7 35.330409257476624 37.0 35.0 37.0 33.0 37.0 8 35.28741230827299 37.0 35.0 37.0 33.0 37.0 9 36.96020500663699 39.0 38.0 39.0 33.0 39.0 10-11 36.96102755854375 39.0 38.0 39.0 33.0 39.0 12-13 36.93888258303475 39.0 38.0 39.0 33.0 39.0 14-15 38.38025918418238 41.0 39.0 41.0 34.0 41.0 16-17 38.38612826740925 41.0 39.0 41.0 34.0 41.0 18-19 38.410838428176845 40.5 39.0 41.0 33.5 41.0 20-21 38.50407983482886 41.0 39.0 41.0 34.0 41.0 22-23 38.5121259085974 41.0 39.0 41.0 34.0 41.0 24-25 38.56064956935391 41.0 39.0 41.0 34.0 41.0 26-27 38.59100467643953 41.0 39.0 41.0 34.0 41.0 28-29 38.55529076248186 41.0 39.0 41.0 34.0 41.0 30-31 38.54172567090592 40.5 39.0 41.0 34.0 41.0 32-33 38.55605742159055 40.0 39.0 41.0 34.0 41.0 34-35 38.50466648292192 40.0 38.5 41.0 33.5 41.0 36-37 38.42158455403657 40.0 38.0 41.0 33.0 41.0 38-39 38.38628689433817 40.0 38.0 41.0 33.0 41.0 40-41 38.33542738982443 40.0 38.0 41.0 33.0 41.0 42-43 38.219833389678705 40.0 38.0 41.0 33.0 41.0 44-45 38.09343465543561 40.0 38.0 41.0 33.0 41.0 46-47 38.00070533543146 40.0 38.0 41.0 33.0 41.0 48-49 37.89485908452575 40.0 37.5 41.0 32.5 41.0 50-51 37.78420164039705 40.0 37.0 41.0 32.0 41.0 52-53 37.674550945493436 40.0 37.0 41.0 32.0 41.0 54-55 37.51086843378423 40.0 36.5 41.0 32.0 41.0 56-57 37.38992037878572 40.0 36.0 41.0 32.0 41.0 58-59 37.18484065121672 39.0 35.5 41.0 31.0 41.0 60-61 37.01455520873358 39.0 35.0 41.0 31.0 41.0 62-63 36.78737316068569 39.0 35.0 41.0 31.0 41.0 64-65 36.54221105573123 38.5 35.0 40.5 31.0 41.0 66-67 36.278320563978696 37.5 35.0 40.0 31.0 41.0 68-69 35.980653737553666 37.0 35.0 40.0 30.0 41.0 70-71 35.70084251038769 36.5 35.0 39.5 30.0 41.0 72-73 35.37048269654012 36.0 35.0 39.0 30.0 41.0 74-75 35.034403261786025 35.5 34.5 39.0 30.0 40.5 76-77 34.71203034775277 35.0 34.0 37.5 29.5 39.5 78-79 34.42078972224221 35.0 34.0 37.0 29.0 39.0 80-81 34.124704978803734 35.0 34.0 37.0 29.0 39.0 82-83 33.86275613949575 35.0 34.0 36.0 29.0 38.0 84-85 33.63208825902759 35.0 34.0 36.0 29.0 37.0 86-87 33.429382051940024 35.0 34.0 35.5 29.0 37.0 88-89 33.284123910939016 35.0 34.0 35.0 29.0 36.5 90-91 33.124045126985266 35.0 34.0 35.0 28.5 36.0 92-93 33.034502601617405 35.0 34.0 35.0 28.0 36.0 94-95 32.94021552443964 35.0 34.0 35.0 28.0 36.0 96-97 32.86705162548476 35.0 34.0 35.0 28.0 35.5 98-99 32.802677265027356 35.0 34.0 35.0 28.0 35.0 100 31.928981524601657 35.0 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23736602583620936 1101 2 0.27529873466913557 1101 3 0.36432670892464714 1101 4 0.3453694679957593 1101 5 0.31537251363582186 1101 6 0.2856080424491765 1101 7 0.2960536495463799 1101 8 0.29334051945244966 1101 9 0.3732079588889121 1101 10-11 0.4373398165218134 1101 12-13 0.3369976276997946 1101 14-15 0.4563975863735976 1101 16-17 0.47009404379691233 1101 18-19 0.38829175039739283 1101 20-21 0.3865128457934688 1101 22-23 0.36600826415924104 1101 24-25 0.38361614098210595 1101 26-27 0.43383426011505577 1101 28-29 0.4772309344093202 1101 30-31 0.4914913715559166 1101 32-33 0.47487802450649497 1101 34-35 0.4477014120036742 1101 36-37 0.48358986988554165 1101 38-39 0.47470663850815953 1101 40-41 0.4317130441739181 1101 42-43 0.42561878120587693 1101 44-45 0.3984963079711079 1101 46-47 0.3807536684007857 1101 48-49 0.3469528503964767 1101 50-51 0.35892480481374633 1101 52-53 0.3134315460774175 1101 54-55 0.33980605566847544 1101 56-57 0.3214060773598675 1101 58-59 0.3352067686910374 1101 60-61 0.3511325239981389 1101 62-63 0.2967626934949479 1101 64-65 0.3042948444507516 1101 66-67 0.24589171148988243 1101 68-69 0.229607201148049 1101 70-71 0.2015484901000164 1101 72-73 0.21616631042451928 1101 74-75 0.18810751224946642 1101 76-77 0.24024028643382422 1101 78-79 0.23067232092921586 1101 80-81 0.21788085611145647 1101 82-83 0.23447848657072257 1101 84-85 0.2807294056153822 1101 86-87 0.2766067930723892 1101 88-89 0.2839244420393925 1101 90-91 0.2689897261459606 1101 92-93 0.2765205256136696 1101 94-95 0.31533171677921956 1101 96-97 0.2553782364398245 1101 98-99 0.2589775301435111 1101 100 0.1330696458987788 1102 1 0.12431070544355549 1102 2 0.12342037099978853 1102 3 0.22895375736148438 1102 4 0.12057809108338091 1102 5 0.1962709031058978 1102 6 0.1717681178356827 1102 7 0.1305749754620038 1102 8 0.20109081676554297 1102 9 0.3094378272648797 1102 10-11 0.3128797673391759 1102 12-13 0.26039818402802695 1102 14-15 0.3672330109770101 1102 16-17 0.32632502079797376 1102 18-19 0.36487607645411657 1102 20-21 0.3025727921763277 1102 22-23 0.28992105567725446 1102 24-25 0.33932417448274066 1102 26-27 0.3611296179993886 1102 28-29 0.389132150873408 1102 30-31 0.34089913898652213 1102 32-33 0.34071196254442526 1102 34-35 0.3672143476429497 1102 36-37 0.4291455430225497 1102 38-39 0.379447648566412 1102 40-41 0.3948140953520891 1102 42-43 0.3491770646510872 1102 44-45 0.2874057736138127 1102 46-47 0.314421227006747 1102 48-49 0.3169812967087893 1102 50-51 0.2919157241663086 1102 52-53 0.2741654306997816 1102 54-55 0.2838138110049897 1102 56-57 0.25186290649050136 1102 58-59 0.2534597770964382 1102 60-61 0.20744979738508817 1102 62-63 0.19874801429073585 1102 64-65 0.2200355571959065 1102 66-67 0.20527244358558505 1102 68-69 0.23223338284162764 1102 70-71 0.2041095400687709 1102 72-73 0.21143235525329374 1102 74-75 0.21110581403738138 1102 76-77 0.21802469916486444 1102 78-79 0.23660976423815328 1102 80-81 0.21197654557548873 1102 82-83 0.2509032713966661 1102 84-85 0.18871767938530581 1102 86-87 0.21048171496470047 1102 88-89 0.21506244570792887 1102 90-91 0.24995677478436562 1102 92-93 0.1894361340472841 1102 94-95 0.20027091877071257 1102 96-97 0.14735632597642478 1102 98-99 0.14044119243614972 1102 100 0.028381673473305113 1103 1 0.18368134409788084 1103 2 0.15870107962323488 1103 3 0.2325545168173413 1103 4 0.14162573636857445 1103 5 0.18397664318094087 1103 6 0.16952664319548916 1103 7 0.08269481042020033 1103 8 0.15132487890682 1103 9 0.22518721091947214 1103 10-11 0.26474626395519607 1103 12-13 0.26075692218362434 1103 14-15 0.34792154653167984 1103 16-17 0.2893492571032752 1103 18-19 0.2345074200137418 1103 20-21 0.23144999916905107 1103 22-23 0.25052008917359103 1103 24-25 0.2566806046650143 1103 26-27 0.2666268405669996 1103 28-29 0.3423675824306258 1103 30-31 0.31451767992602697 1103 32-33 0.3264445225128867 1103 34-35 0.37279405924308406 1103 36-37 0.34306050094559737 1103 38-39 0.2910511308608079 1103 40-41 0.29424200524672983 1103 42-43 0.318189459380541 1103 44-45 0.2804734360852521 1103 46-47 0.2735986857805699 1103 48-49 0.2707293273015523 1103 50-51 0.22069098855570246 1103 52-53 0.29742346579714507 1103 54-55 0.3046567894534036 1103 56-57 0.27651063482088745 1103 58-59 0.257737292941286 1103 60-61 0.261389489692192 1103 62-63 0.23975795133464572 1103 64-65 0.25645253052212524 1103 66-67 0.2602964071621088 1103 68-69 0.23785620434833277 1103 70-71 0.19466617073315717 1103 72-73 0.2512748525783124 1103 74-75 0.22628404854901873 1103 76-77 0.22960215918929805 1103 78-79 0.19181028903663844 1103 80-81 0.21592439389210938 1103 82-83 0.19745000854793204 1103 84-85 0.19383445552428213 1103 86-87 0.14494269202157284 1103 88-89 0.1463927085977943 1103 90-91 0.18324375845455165 1103 92-93 0.10602885149136654 1103 94-95 0.12944337150433682 1103 96-97 0.08738625218813922 1103 98-99 0.11328561894452349 1103 100 0.11807291635415851 1104 1 0.10027130031078002 1104 2 0.15669348620658852 1104 3 0.17523233897640367 1104 4 0.1815706865039104 1104 5 0.15042884517694688 1104 6 0.15979579317285442 1104 7 0.10930149520201127 1104 8 0.19902110374452775 1104 9 0.22011044005405722 1104 10-11 0.26621742756808686 1104 12-13 0.24834292656361612 1104 14-15 0.23508435806678563 1104 16-17 0.27905232194418517 1104 18-19 0.2419154160880126 1104 20-21 0.2793775868545154 1104 22-23 0.28255525165676687 1104 24-25 0.28563697983404523 1104 26-27 0.2691780961008945 1104 28-29 0.3033926307511621 1104 30-31 0.28865553903992947 1104 32-33 0.2597998213883912 1104 34-35 0.27519339063563564 1104 36-37 0.27954968369673594 1104 38-39 0.2814344602756904 1104 40-41 0.2321331890537266 1104 42-43 0.21989184062545064 1104 44-45 0.18747750175590738 1104 46-47 0.13254726001680694 1104 48-49 0.135126552838031 1104 50-51 0.12698400960950806 1104 52-53 0.15489818565817615 1104 54-55 0.18020990127160985 1104 56-57 0.14638619546399667 1104 58-59 0.20971000468213674 1104 60-61 0.16993084908637712 1104 62-63 0.14119559858448127 1104 64-65 0.1552134387514883 1104 66-67 0.09271368067160068 1104 68-69 0.1315438439743417 1104 70-71 0.10243989408104426 1104 72-73 0.07410234888890699 1104 74-75 0.0677399343267524 1104 76-77 0.11890398438889349 1104 78-79 0.08162343025846042 1104 80-81 0.106071830297509 1104 82-83 0.14162215765465191 1104 84-85 0.1377888806736678 1104 86-87 0.1577235709464162 1104 88-89 0.10673215353025967 1104 90-91 0.18534641123581963 1104 92-93 0.18495291046274076 1104 94-95 0.1644129882421197 1104 96-97 0.13302516857231694 1104 98-99 0.1008287419696714 1104 100 0.07977101117200647 1105 1 0.11276510582939636 1105 2 0.1292259648457268 1105 3 0.16662195103048916 1105 4 0.1336355842231356 1105 5 0.11549079365109804 1105 6 0.12308652653199914 1105 7 0.05034734066525459 1105 8 0.19454575598946633 1105 9 0.17948957117899056 1105 10-11 0.19152581299642435 1105 12-13 0.19022087317455316 1105 14-15 0.27130498163595007 1105 16-17 0.2552034182935259 1105 18-19 0.2042291770476865 1105 20-21 0.22170995230531076 1105 22-23 0.21546956082116964 1105 24-25 0.19888246086281214 1105 26-27 0.2084840224044413 1105 28-29 0.22048687372291909 1105 30-31 0.2013953956077188 1105 32-33 0.182895867760152 1105 34-35 0.18679885379564354 1105 36-37 0.16440164127835288 1105 38-39 0.1338161844417698 1105 40-41 0.14246217203140077 1105 42-43 0.13079272646569962 1105 44-45 0.1598666647901652 1105 46-47 0.12185416154100892 1105 48-49 0.057905574058466414 1105 50-51 0.07720401468483828 1105 52-53 0.1036871655287328 1105 54-55 0.1332876819956681 1105 56-57 0.04644769972905749 1105 58-59 0.060824804782555475 1105 60-61 2.772662903751666E-4 1105 62-63 0.01790009736367182 1105 64-65 0.01611904905216477 1105 66-67 0.03137266702672292 1105 68-69 -0.0010631059657839614 1105 70-71 0.03401914446003218 1105 72-73 0.04575457976238084 1105 74-75 0.04679122711639394 1105 76-77 0.07188019052115635 1105 78-79 0.044930125339170957 1105 80-81 0.06411548431180591 1105 82-83 0.03026671701884709 1105 84-85 0.050920799478625156 1105 86-87 0.07293346797722933 1105 88-89 0.03415283292493143 1105 90-91 -0.006817264571132853 1105 92-93 0.10470111319380493 1105 94-95 -0.007930125029311341 1105 96-97 0.007150077972227109 1105 98-99 -0.06694267802011211 1105 100 -0.10466766201125566 1106 1 0.10679491718213185 1106 2 0.10668234992461834 1106 3 0.16895828308174643 1106 4 0.20631690170641548 1106 5 0.10355785188621525 1106 6 0.13814536988562764 1106 7 0.12765867874987435 1106 8 0.17408436020070184 1106 9 0.17122557087684243 1106 10-11 0.1924176456875486 1106 12-13 0.20880721058086493 1106 14-15 0.25010455082825445 1106 16-17 0.22947781523883037 1106 18-19 0.17095707768103097 1106 20-21 0.20707026846994125 1106 22-23 0.18799234887296734 1106 24-25 0.18916009960056357 1106 26-27 0.19211534402649733 1106 28-29 0.24602503741596138 1106 30-31 0.23301748446859705 1106 32-33 0.2116647075334015 1106 34-35 0.24630001789920897 1106 36-37 0.2553232978296549 1106 38-39 0.222169690301385 1106 40-41 0.2711812602325807 1106 42-43 0.20884862697457152 1106 44-45 0.1957535223240896 1106 46-47 0.12201830947535086 1106 48-49 0.14663394633090832 1106 50-51 0.15906104658586884 1106 52-53 0.09472199724275043 1106 54-55 0.22868076324024855 1106 56-57 0.13342421689840478 1106 58-59 0.1910174943175349 1106 60-61 0.20843754095392342 1106 62-63 0.14003595965687765 1106 64-65 0.14372591004681112 1106 66-67 0.1352360599002651 1106 68-69 0.1434984122334697 1106 70-71 0.140451906762749 1106 72-73 0.1454540637218571 1106 74-75 0.1419612301610158 1106 76-77 0.19556489579916558 1106 78-79 0.16638867735791507 1106 80-81 0.14597628321958211 1106 82-83 0.12954515795668442 1106 84-85 0.18359285652677215 1106 86-87 0.14517035252772814 1106 88-89 0.11467873267202577 1106 90-91 0.12659152543626817 1106 92-93 0.10238844188425134 1106 94-95 0.03348983757538804 1106 96-97 0.07317660872988085 1106 98-99 0.048189555488413305 1106 100 0.03045784899059356 1107 1 0.030649444119198677 1107 2 0.06579791367915888 1107 3 0.144886778024631 1107 4 0.09198630513187567 1107 5 0.08186814072392679 1107 6 0.0437898632225 1107 7 0.03684502116976773 1107 8 0.01174922422673319 1107 9 0.04281793163660552 1107 10-11 0.11980498146098029 1107 12-13 0.09616363871474931 1107 14-15 0.16439575339944668 1107 16-17 0.12934272394149104 1107 18-19 0.08643426057425785 1107 20-21 0.08783825999431372 1107 22-23 0.11975004000617417 1107 24-25 0.14283453286798675 1107 26-27 0.12683218483483216 1107 28-29 0.1481254856584755 1107 30-31 0.1659793218176091 1107 32-33 0.18040288795990023 1107 34-35 0.19684956363595774 1107 36-37 0.1879797432022272 1107 38-39 0.1377642988007537 1107 40-41 0.14544802119760902 1107 42-43 0.11270643328857233 1107 44-45 0.07971259892084248 1107 46-47 0.05726387661296428 1107 48-49 0.12407751829462654 1107 50-51 0.12527647358989924 1107 52-53 0.16358312990391966 1107 54-55 0.18942570640565037 1107 56-57 0.1300299641772753 1107 58-59 0.1587405319038453 1107 60-61 0.15391940013127225 1107 62-63 0.11061198197030109 1107 64-65 0.1520368369478149 1107 66-67 0.10704021375330797 1107 68-69 0.07935206711002962 1107 70-71 0.10194494558982115 1107 72-73 0.12006779447793292 1107 74-75 0.09938664599101799 1107 76-77 0.016726905437458583 1107 78-79 0.08516210986637418 1107 80-81 0.07479848059976035 1107 82-83 0.09092767641324428 1107 84-85 0.0921818594632029 1107 86-87 0.07578544676285048 1107 88-89 0.028909276530093564 1107 90-91 0.07886605027736238 1107 92-93 0.03662974863787127 1107 94-95 0.04761636369651967 1107 96-97 0.032341058136765355 1107 98-99 -0.013821546812899044 1107 100 -0.004456437636953581 1108 1 0.02947063306142539 1108 2 0.02565191781713594 1108 3 0.07555174803086473 1108 4 -0.010079877179613561 1108 5 0.004917136176274539 1108 6 -0.07281405709115063 1108 7 -0.10230113493854276 1108 8 -0.09802398759103426 1108 9 -0.06524807088635498 1108 10-11 -0.015046017318759652 1108 12-13 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2214 80-81 -0.18728412844215114 2214 82-83 -0.18164145909743468 2214 84-85 -0.22384649682480529 2214 86-87 -0.21598121071659193 2214 88-89 -0.2706195468384749 2214 90-91 -0.2390714798418685 2214 92-93 -0.2221213496386767 2214 94-95 -0.19018761678795215 2214 96-97 -0.18246206832269962 2214 98-99 -0.20621294872206164 2214 100 -0.15627042502126542 2215 1 -0.3228553566771133 2215 2 -0.33860026182689396 2215 3 -0.4088816529173229 2215 4 -0.3600430260433427 2215 5 -0.31754511195767776 2215 6 -0.39087518410636335 2215 7 -0.32212112393955294 2215 8 -0.2944033589659938 2215 9 -0.39920656703839796 2215 10-11 -0.4256831873553608 2215 12-13 -0.45251119442705345 2215 14-15 -0.5937759275489825 2215 16-17 -0.516113478336159 2215 18-19 -0.5503820278779799 2215 20-21 -0.5318469746539662 2215 22-23 -0.4683140596573878 2215 24-25 -0.4547617954073573 2215 26-27 -0.4712853724863848 2215 28-29 -0.4140795038979945 2215 30-31 -0.45027390907272746 2215 32-33 -0.5111796977053586 2215 34-35 -0.5776086842000083 2215 36-37 -0.5098642882285347 2215 38-39 -0.5157653867453007 2215 40-41 -0.4857862042347776 2215 42-43 -0.470173862529478 2215 44-45 -0.42361369389490733 2215 46-47 -0.3516929167830085 2215 48-49 -0.31202775854797693 2215 50-51 -0.38887640187903827 2215 52-53 -0.38277071491654624 2215 54-55 -0.3650120333199425 2215 56-57 -0.3497062518474934 2215 58-59 -0.26297152949891256 2215 60-61 -0.3180531397865636 2215 62-63 -0.24562148369399495 2215 64-65 -0.2734482046255664 2215 66-67 -0.27603479843111955 2215 68-69 -0.2627970616704687 2215 70-71 -0.32991221463345255 2215 72-73 -0.3164432923205922 2215 74-75 -0.2612151484874019 2215 76-77 -0.2942541258736142 2215 78-79 -0.2619102504460429 2215 80-81 -0.21318746612942618 2215 82-83 -0.2183804112335963 2215 84-85 -0.17038567078776623 2215 86-87 -0.17878820703128895 2215 88-89 -0.24028716460364308 2215 90-91 -0.22208823719712 2215 92-93 -0.2739663241302779 2215 94-95 -0.23544669855532163 2215 96-97 -0.220804353595625 2215 98-99 -0.2194690343067407 2215 100 -0.10642984612917417 2216 1 -0.21691424188846398 2216 2 -0.298119303102073 2216 3 -0.28649962974025556 2216 4 -0.26786565753445046 2216 5 -0.25110508099572826 2216 6 -0.24049546618284268 2216 7 -0.2307839254469357 2216 8 -0.19520728935551546 2216 9 -0.26476357895789704 2216 10-11 -0.31891798186891407 2216 12-13 -0.3540948863435389 2216 14-15 -0.4830050652038551 2216 16-17 -0.45396819712207304 2216 18-19 -0.49834826674867827 2216 20-21 -0.48842300179918396 2216 22-23 -0.5117510162372412 2216 24-25 -0.5718187350826653 2216 26-27 -0.5324846253235762 2216 28-29 -0.49477044417171356 2216 30-31 -0.5493948849174757 2216 32-33 -0.5382456598395748 2216 34-35 -0.5349143966946386 2216 36-37 -0.5244544349424345 2216 38-39 -0.5166814978862106 2216 40-41 -0.5070084787199107 2216 42-43 -0.4711038594226764 2216 44-45 -0.4183240627961524 2216 46-47 -0.34173636087381 2216 48-49 -0.3716473498902886 2216 50-51 -0.3509418728184315 2216 52-53 -0.3441383653012977 2216 54-55 -0.32280525052355813 2216 56-57 -0.3063153533239671 2216 58-59 -0.3108483255376058 2216 60-61 -0.3367079988076398 2216 62-63 -0.3380558957917472 2216 64-65 -0.35270617899716683 2216 66-67 -0.3449864558405338 2216 68-69 -0.33702976725300005 2216 70-71 -0.3242592715728563 2216 72-73 -0.290369997470485 2216 74-75 -0.32497161431415833 2216 76-77 -0.3124463474359871 2216 78-79 -0.29959748054515245 2216 80-81 -0.22892445161242136 2216 82-83 -0.22707179196993366 2216 84-85 -0.23042145202069264 2216 86-87 -0.23716930817253257 2216 88-89 -0.3158490619572092 2216 90-91 -0.2854035092121734 2216 92-93 -0.24909293048357029 2216 94-95 -0.2529981254503042 2216 96-97 -0.28836018539018227 2216 98-99 -0.310958572895764 2216 100 -0.28692297730125915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 118.0 1 64.0 2 49.0 3 52.0 4 47.0 5 65.0 6 152.0 7 881.0 8 2394.0 9 2935.0 10 2906.0 11 3667.0 12 4633.0 13 5749.0 14 6977.0 15 8027.0 16 8755.0 17 9333.0 18 10971.0 19 12119.0 20 13876.0 21 15662.0 22 17545.0 23 20538.0 24 25186.0 25 31789.0 26 36237.0 27 34790.0 28 36896.0 29 46281.0 30 53050.0 31 58340.0 32 74419.0 33 101298.0 34 149502.0 35 233296.0 36 439707.0 37 1024137.0 38 1439571.0 39 485936.0 40 1224.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.45057214025537 20.023403216176114 13.300616530740383 24.225408112828138 2 15.020116530587583 20.929089965180232 40.2400993583259 23.81069414590629 3 17.742377889395378 26.10079802510174 30.84914016880238 25.3076839167005 4 12.148286112264655 17.786978458864546 40.069997091941055 29.994738336929743 5 13.102985034768603 38.50929765298931 35.75202516972409 12.635692142517996 6 29.835071872598252 38.651267400103016 18.707583217687205 12.80607750961153 7 26.361568657800937 32.93394785608364 23.426315087963694 17.278168398151724 8 25.101648025255013 35.6630402190578 21.199907217253063 18.035404538434122 9 25.90619649347998 15.819056253046604 20.94167401774306 37.33307323573035 10-11 23.96535401103428 26.61434781974065 29.61701830096021 19.80327986826486 12-13 24.735938630971262 24.463427773364295 28.64752852317649 22.153105072487953 14-15 22.3298085974377 25.882859608663 26.123257089971798 25.664074703927504 16-17 22.148351423286808 28.586211352483993 26.857272564125022 22.40816466010418 18-19 21.926503553704542 28.505832809723003 27.93224329600566 21.635420340566792 20-21 23.140697575740433 27.716217363016604 27.33092698933552 21.812158071907444 22-23 23.023806177915997 27.597920645168443 26.930686437373712 22.447586739541844 24-25 22.746994833350286 27.669438513949206 27.243144370764117 22.34042228193639 26-27 22.63427814623488 27.897459861811143 26.937752781909285 22.530509210044688 28-29 22.473919479256317 28.016076811685053 27.112528403491265 22.397475305567365 30-31 22.91419001872691 27.724754032509008 27.040876713568807 22.320179235195276 32-33 22.77865692930132 27.87477361926849 26.800932623549706 22.54563682788049 34-35 22.995345988824898 27.845083856276386 27.066692028796503 22.092878126102214 36-37 22.997136737909106 27.622897224711192 26.947198151723693 22.43276788565601 38-39 22.771037012803085 27.73055866223349 27.210138084368385 22.288266240595046 40-41 22.835992228818913 27.41939572367766 27.320015317845293 22.424596729658138 42-43 22.68434080397565 27.47049972227843 27.18241169155749 22.66274778218843 44-45 22.705519582449913 27.834707006241118 27.076200989551513 22.383572421757457 46-47 22.72305225046997 27.534176653602255 26.948089061320484 22.79468203460729 48-49 22.814885236995792 27.433292589071485 27.15549594820952 22.59632622572321 50-51 22.72631842118696 27.54282727649408 27.019283125474146 22.71157117684481 52-53 22.644484504206126 27.645826787908312 26.946096331151576 22.76359237673399 54-55 22.674796586013375 27.600375951423313 27.004689432613965 22.720138029949347 56-57 22.927379079545457 27.47278680686876 26.940673535514193 22.659160578071596 58-59 22.65535328511485 27.464593052268988 27.23112920690769 22.648924455708478 60-61 22.75366944648177 27.639952824636225 27.04773675965918 22.55864096922283 62-63 22.820257208087565 27.411693655542894 26.936326805570083 22.83172233079946 64-65 22.47811172516141 27.463101522932266 27.06732656323639 22.991460188669937 66-67 22.79947210737137 27.385720413847903 26.897555553794906 22.917251924985823 68-69 22.67453245031913 27.373051616941467 27.059431413407527 22.892984519331876 70-71 22.796546928511045 27.168019801431072 27.011753477706694 23.02367979235119 72-73 22.947777094530654 27.29222147483815 27.01975640561156 22.740245025019632 74-75 22.845934561911335 27.55539930897864 26.87689628499617 22.721769844113858 76-77 22.744364199943384 27.427762122384618 26.812136753471755 23.01573692420024 78-79 22.714542546318537 27.453876142541972 26.922078286620536 22.909503024518955 80-81 22.748716996765044 27.488222630207567 26.957431684463835 22.805628688563555 82-83 23.044101912618807 27.24555659447209 27.219195706559514 22.491145786349588 84-85 23.141801289385587 27.39003263134055 26.7820332615348 22.686132817739065 86-87 22.659841163308432 27.541720945867176 27.00305246228562 22.79538542853877 88-89 23.017269049215386 27.18319063181469 26.9175521283449 22.881988190625023 90-91 23.00665406899624 27.243529961419256 26.94895679880337 22.80085917078113 92-93 23.062251233483522 27.2777185899857 26.940244425145693 22.71978575138509 94-95 22.639601078977417 27.31618855483489 27.127507857356942 22.916702508830756 96-97 22.798695620581867 27.296998256798226 27.018937088321092 22.885369034298815 98-99 22.701164645762255 27.440862288764784 26.91510571720376 22.942867348269193 100 22.98839205137439 27.092245424940824 26.75342089638995 23.165941627294828 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1199.0 1 868.0 2 589.5 3 646.0 4 616.5 5 556.5 6 515.5 7 488.0 8 493.5 9 515.0 10 475.5 11 424.5 12 432.5 13 477.5 14 543.0 15 649.0 16 797.0 17 956.5 18 1190.0 19 1596.0 20 2243.5 21 3306.5 22 4875.5 23 6849.5 24 9902.5 25 14440.0 26 20618.5 27 27978.5 28 34661.0 29 42009.5 30 50496.5 31 58581.5 32 68636.5 33 79406.0 34 89234.5 35 99128.0 36 110228.5 37 124033.0 38 132206.0 39 134057.0 40 137608.0 41 141317.5 42 145260.5 43 153137.5 44 162192.0 45 172168.0 46 180309.5 47 183778.0 48 184995.5 49 182105.5 50 175623.0 51 170547.5 52 165367.0 53 159556.0 54 150634.5 55 136024.5 56 123495.0 57 109603.5 58 95113.5 59 84254.5 60 69346.5 61 53959.5 62 42244.0 63 31994.0 64 23095.5 65 15996.0 66 11851.0 67 8632.0 68 6091.0 69 4375.5 70 3221.0 71 2271.0 72 1572.0 73 1297.5 74 1095.0 75 861.5 76 613.5 77 355.0 78 221.5 79 162.0 80 101.0 81 75.5 82 60.5 83 51.5 84 35.5 85 24.5 86 23.0 87 17.5 88 10.5 89 12.0 90 13.5 91 11.0 92 9.5 93 7.5 94 5.5 95 5.0 96 9.0 97 7.0 98 4.0 99 6.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06080321797693416 2 0.00950403853751855 3 0.011676390203237077 4 0.009481409874333982 5 0.012038448814190163 6 0.011812162182344483 7 0.0030548695299166765 8 0.005634537132957426 9 0.007241172219061753 10-11 0.006958313929254652 12-13 0.006053167401871934 14-15 0.010194212764647874 16-17 0.009571924527072253 18-19 0.007569287835237987 20-21 0.006765970292185825 22-23 0.0025117816134870453 24-25 0.0036092717779385923 26-27 0.005012248895381806 28-29 0.005951338417541378 30-31 0.009028836610642622 32-33 0.01032998474375528 34-35 0.011597189882091088 36-37 0.017808757926254994 38-39 0.018657332795676298 40-41 0.018057673221285243 42-43 0.024438956239333412 44-45 0.027448568442880957 46-47 0.029847206740445158 48-49 0.0310917832155964 50-51 0.031668814126802884 52-53 0.030096122035475407 54-55 0.035798545157986535 56-57 0.038321641103065865 58-59 0.03560620152091771 60-61 0.03652266237989271 62-63 0.03231373102756307 64-65 0.03421453873506678 66-67 0.03640951906396987 68-69 0.039124958646118035 70-71 0.03881947169312636 72-73 0.03492734162538067 74-75 0.037450437570459995 76-77 0.03696392131199179 78-79 0.03647740505352358 80-81 0.04082210838496063 82-83 0.037212836607022036 84-85 0.03368276515022943 86-87 0.032336359690747635 88-89 0.03005086470910627 90-91 0.032268473701193935 92-93 0.03399956643481339 94-95 0.0375183235600137 96-97 0.032607903648962454 98-99 0.03054869529916677 100 0.028353714970263674 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4419174.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.30166663990802 #Duplication Level Percentage of deduplicated Percentage of total 1 70.53342680093286 41.122163363183425 2 15.666057664633021 18.26714543050021 3 5.975674693386354 10.451753817670376 4 3.0133951120602807 7.02743829110667 5 1.5962887163583133 4.653314630108453 6 0.9493272944598129 3.32084180722571 7 0.6088191889110571 2.484662137711015 8 0.4032739175997392 1.8809233206775786 9 0.28296859846392136 1.4847786807484982 >10 0.9516276819518745 8.349143579696875 >50 0.01526766270954133 0.5955649095216051 >100 0.003807399265097759 0.34004703828823424 >500 6.526926820422825E-5 0.022222993561374197 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.3942994776851965E-5 14-15 0.0 0.0 0.0 0.0 4.525732636913595E-5 16-17 0.0 0.0 0.0 2.2628663184567976E-5 4.525732636913595E-5 18-19 0.0 0.0 0.0 2.2628663184567976E-5 4.525732636913595E-5 20-21 0.0 0.0 0.0 1.0182898433055588E-4 4.525732636913595E-5 22-23 0.0 0.0 0.0 3.2811561617623566E-4 4.525732636913595E-5 24-25 0.0 0.0 0.0 7.920032114598791E-4 4.525732636913595E-5 26-27 0.0 0.0 0.0 0.0013124624647049424 4.525732636913595E-5 28-29 0.0 0.0 0.0 0.004435217984175324 4.525732636913595E-5 30-31 0.0 0.0 0.0 0.012728623041319487 4.525732636913595E-5 32-33 0.0 0.0 0.0 0.02938331914516152 4.525732636913595E-5 34-35 0.0 0.0 0.0 0.05685451625122704 4.525732636913595E-5 36-37 0.0 0.0 0.0 0.10138772539845681 4.525732636913595E-5 38-39 0.0 0.0 0.0 0.16141025449552338 5.657165796141994E-5 40-41 0.0 0.0 0.0 0.22193061418265042 6.788598955370393E-5 42-43 0.0 0.0 0.0 0.2862639036163772 6.788598955370393E-5 44-45 0.0 0.0 0.0 0.36549816775714195 6.788598955370393E-5 46-47 0.0 0.0 0.0 0.458049400182025 6.788598955370393E-5 48-49 0.0 0.0 0.0 0.553587616147271 6.788598955370393E-5 50-51 0.0 0.0 0.0 0.6495105193866546 6.788598955370393E-5 52-53 0.0 0.0 0.0 0.7450600496834929 9.05146527382719E-5 54-55 0.0 0.0 0.0 0.8491519003325055 9.05146527382719E-5 56-57 0.0 0.0 0.0 0.9716295398189798 9.05146527382719E-5 58-59 0.0 0.0 0.0 1.1021064117411987 9.05146527382719E-5 60-61 0.0 0.0 0.0 1.2372334739478463 1.1314331592283987E-4 62-63 0.0 0.0 0.0 1.3728131094181855 1.3577197910740786E-4 64-65 0.0 0.0 0.0 1.5130542495045454 1.3577197910740786E-4 66-67 0.0 0.0 0.0 1.6666463008698005 1.3577197910740786E-4 68-69 0.0 0.0 0.0 1.835705043521708 1.3577197910740786E-4 70-71 0.0 0.0 0.0 2.0155124011862853 1.5840064229197585E-4 72-73 0.0 0.0 0.0 2.1944938126446254 2.0365796866111176E-4 74-75 0.0 0.0 0.0 2.375115802183847 2.3760096343796374E-4 76-77 0.0 0.0 0.0 2.5728224324274174 2.4891529503024773E-4 78-79 0.0 0.0 0.0 2.787636332038521 2.4891529503024773E-4 80-81 0.0 2.2628663184567976E-5 0.0 3.0152127976857215 2.4891529503024773E-4 82-83 0.0 2.2628663184567976E-5 0.0 3.249973863894022 2.4891529503024773E-4 84-85 0.0 4.525732636913595E-5 0.0 3.4857871629404045 2.4891529503024773E-4 86-87 0.0 4.525732636913595E-5 0.0 3.7322019906887576 2.4891529503024773E-4 88 0.0 4.525732636913595E-5 0.0 3.9247832287210236 2.4891529503024773E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4130 0.0 35.868706 1 GTATCAA 6465 0.0 30.260773 1 CAACGCA 6225 0.0 29.746223 5 ATCAACG 6460 0.0 28.810278 3 AACGCAG 7445 0.0 26.133396 6 TCAACGC 7315 0.0 25.699842 4 TATCAAC 7800 0.0 25.246675 2 ACGCAGA 7740 0.0 24.041706 7 GTACATG 12690 0.0 24.014227 1 CGCAGAG 7965 0.0 23.423414 8 TACATGG 12790 0.0 22.892979 2 ACATGGG 12490 0.0 22.539755 3 GTACTAG 1880 0.0 22.013039 1 TATTCGC 330 1.8189894E-12 21.361061 9 GCTATTG 2145 0.0 19.936989 8 CATGGGG 9865 0.0 19.34255 4 TGATCGC 1155 0.0 18.716358 9 GTATAAG 2445 0.0 18.464926 1 GCAGAGT 9820 0.0 18.28089 9 GAGTACA 9305 0.0 17.891308 1 >>END_MODULE