FastQCFastQC Report
Mon 16 Jan 2017
ERR1146542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4419174
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135540.30670890080363433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118880.2690095479381441No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70200.15885321555566717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74400.054.4593051
GTATCAA138400.043.8287121
CAACGCA171350.034.7108085
ATCAACG172800.034.419933
TATCAAC181550.033.0206452
TCAACGC181550.032.890144
AACGCAG184400.032.497896
ACGCAGA197800.029.9846977
CGCAGAG200100.029.6400458
GCAGAGT226750.025.9284539
AGAGTAC207150.023.39054310-11
GTACATG121950.022.998861
GTGGTAT26650.022.7789861
TACATGG120850.022.6225642
GAGTACT161350.022.45701212-13
ACATGGG119300.022.1492523
CAGAGTA219450.022.1437710-11
AGTACTT177300.020.76810512-13
GTACTAG18500.020.34981
GTACTTT180150.020.07432614-15
Produced by FastQC (version 0.11.5)