FastQCFastQC Report
Mon 16 Jan 2017
ERR1146541_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146541_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2710157
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA131230.48421549009891307No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63040.23260645047500939No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC49860.18397458154638274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15350.028.7930661
GTACCCG2600.027.1260361
TATTCTC12400.026.9079535
GTGATCG13100.026.1866258
AGGGCTA25100.025.2757475
CTAACGC8350.024.7633933
TGATCGC13950.024.5910229
GCTATTG25150.024.4771738
TAACGCC8450.024.4703374
CGTTAAC5700.023.9216751
GGGCACC18400.023.75687294
CGCCTAC8750.023.6300457
GGTGATC14800.023.4957837
GGGCTAT27200.023.323886
TCTAACG8800.022.963062
CATGGGG77050.022.7499354
TTTAGGG29200.022.5314522
TAAGGTG18000.022.4527684
ATTTAGG28750.022.405321
CTATTGA27400.022.2956819
Produced by FastQC (version 0.11.5)