##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146541_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2710157
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.222512570304968	31.0	31.0	34.0	28.0	34.0
2	31.425622205650818	33.0	31.0	34.0	28.0	34.0
3	31.601638945640417	33.0	31.0	34.0	28.0	34.0
4	35.04391848885508	37.0	35.0	37.0	32.0	37.0
5	35.11770904785221	37.0	35.0	37.0	32.0	37.0
6	35.17092072525688	37.0	35.0	37.0	32.0	37.0
7	35.08302065157111	37.0	35.0	37.0	32.0	37.0
8	35.04468634104961	37.0	35.0	37.0	32.0	37.0
9	36.61128635721104	39.0	37.0	39.0	32.0	39.0
10-11	36.584758373776864	39.0	37.0	39.0	32.0	39.0
12-13	36.54670947107492	39.0	37.0	39.0	32.0	39.0
14-15	37.92515193769217	40.0	38.0	41.0	32.0	41.0
16-17	37.96827305576762	40.0	38.0	41.0	32.0	41.0
18-19	38.029179674830644	40.0	38.5	41.0	32.0	41.0
20-21	38.115414162352955	40.0	39.0	41.0	32.0	41.0
22-23	38.14914597198612	40.0	38.5	41.0	32.0	41.0
24-25	38.214061214903786	40.5	38.0	41.0	32.0	41.0
26-27	38.22965385400181	40.0	38.0	41.0	32.0	41.0
28-29	38.215991361386074	40.0	38.0	41.0	32.0	41.0
30-31	38.215917749414515	40.0	38.0	41.0	32.5	41.0
32-33	38.19770570487245	40.0	38.0	41.0	32.0	41.0
34-35	38.12191821359427	40.0	38.0	41.0	32.0	41.0
36-37	38.02973517770373	40.0	38.0	41.0	32.0	41.0
38-39	37.96771699942107	40.0	38.0	41.0	32.0	41.0
40-41	37.907175303866154	40.0	38.0	41.0	32.0	41.0
42-43	37.76215363168997	40.0	37.5	41.0	31.0	41.0
44-45	37.60893501741781	40.0	37.0	41.0	31.0	41.0
46-47	37.46413934690868	40.0	37.0	41.0	31.0	41.0
48-49	37.31790796621746	40.0	36.0	41.0	30.0	41.0
50-51	37.15184784497725	40.0	36.0	41.0	30.0	41.0
52-53	37.03864646955878	39.5	35.0	41.0	30.0	41.0
54-55	36.8427753447494	39.0	35.0	41.0	30.0	41.0
56-57	36.633395961931356	39.0	35.0	41.0	30.0	41.0
58-59	36.366400175340395	38.5	35.0	41.0	30.0	41.0
60-61	36.12657144955071	38.0	35.0	40.0	30.0	41.0
62-63	35.87376635375736	37.0	35.0	40.0	29.0	41.0
64-65	35.634630023279094	37.0	35.0	40.0	29.0	41.0
66-67	35.332117475113066	36.0	34.0	39.5	29.0	41.0
68-69	35.01431559131076	36.0	34.0	39.0	28.5	41.0
70-71	34.7186227587553	35.0	34.0	39.0	28.0	41.0
72-73	34.413685258824486	35.0	34.0	38.5	28.0	40.5
74-75	34.14443775766496	35.0	34.0	37.0	28.0	40.0
76-77	33.85902182050708	35.0	34.0	37.0	27.5	39.0
78-79	33.58681360526346	35.0	33.0	36.0	27.0	39.0
80-81	33.33128283711977	35.0	33.0	36.0	27.0	39.0
82-83	33.08665918616523	35.0	33.0	36.0	26.5	37.0
84-85	32.90451567934994	35.0	33.0	35.0	26.0	37.0
86-87	32.77405552519651	35.0	33.0	35.0	26.0	36.5
88-89	32.641457303027096	35.0	33.0	35.0	26.0	36.0
90-91	32.45019716569925	35.0	33.0	35.0	25.5	36.0
92-93	32.30502033646022	35.0	33.0	35.0	25.0	36.0
94-95	32.22511574052721	35.0	33.0	35.0	25.0	36.0
96-97	32.161686020403984	35.0	33.0	35.0	25.0	35.0
98-99	32.128505285856136	35.0	33.0	35.0	25.0	35.0
100	31.109474838542564	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2716268955530161
1101	2	0.2839013396972234
1101	3	0.40225426427819855
1101	4	0.4535706900505758
1101	5	0.3914592118690621
1101	6	0.394646740539045
1101	7	0.39085061412468747
1101	8	0.44344101088244514
1101	9	0.5213417332218313
1101	10-11	0.5115816044999377
1101	12-13	0.4437816198175639
1101	14-15	0.6206353772997915
1101	16-17	0.5731151818263598
1101	18-19	0.5281397175624178
1101	20-21	0.48639115394232135
1101	22-23	0.45513216796948797
1101	24-25	0.5134246050413864
1101	26-27	0.5128750856675452
1101	28-29	0.4869196373221172
1101	30-31	0.5266305032623961
1101	32-33	0.5438343951992479
1101	34-35	0.45504699834626194
1101	36-37	0.47782713138791877
1101	38-39	0.5106889428151788
1101	40-41	0.40830858185759666
1101	42-43	0.41515637268093997
1101	44-45	0.35145062178777664
1101	46-47	0.3674160524866181
1101	48-49	0.43086552903951514
1101	50-51	0.3720408952729244
1101	52-53	0.35595279714316064
1101	54-55	0.44179210425824067
1101	56-57	0.32010326031553404
1101	58-59	0.4177854389162192
1101	60-61	0.45586817925925516
1101	62-63	0.4584618717540394
1101	64-65	0.39808250896382447
1101	66-67	0.41422312864512634
1101	68-69	0.3874862262666241
1101	70-71	0.40108767668009193
1101	72-73	0.4777789788626592
1101	74-75	0.4426127984504049
1101	76-77	0.23560490107357168
1101	78-79	0.27192095500959823
1101	80-81	0.323889704815187
1101	82-83	0.4242161428471576
1101	84-85	0.4704619040312963
1101	86-87	0.4069782216694051
1101	88-89	0.2990817264530534
1101	90-91	0.33978716507812834
1101	92-93	0.44453741600577956
1101	94-95	0.4515666748602598
1101	96-97	0.4042619673842083
1101	98-99	0.3171039461035363
1101	100	0.2357184275419364
1102	1	0.1430818995317864
1102	2	0.2608071780879513
1102	3	0.2892303012352393
1102	4	0.25100923514298756
1102	5	0.29406821592628063
1102	6	0.22649326963393435
1102	7	0.18843026102779703
1102	8	0.27394881785118486
1102	9	0.4125332906467776
1102	10-11	0.35334074354289413
1102	12-13	0.35857008676384794
1102	14-15	0.41799497850518463
1102	16-17	0.4000029960811986
1102	18-19	0.2890958825980192
1102	20-21	0.3008318855311032
1102	22-23	0.3185331088476602
1102	24-25	0.3216706513653236
1102	26-27	0.34901150868179087
1102	28-29	0.4172649528417267
1102	30-31	0.3956220424720769
1102	32-33	0.3993054641759244
1102	34-35	0.4569772179332574
1102	36-37	0.4784052499373743
1102	38-39	0.46903357758917963
1102	40-41	0.43156358124931415
1102	42-43	0.39358449850985266
1102	44-45	0.4166035103362873
1102	46-47	0.38910011007609313
1102	48-49	0.35239119195210833
1102	50-51	0.37434456099493474
1102	52-53	0.339163676042908
1102	54-55	0.4376872298804031
1102	56-57	0.38151553820067363
1102	58-59	0.39268128284465575
1102	60-61	0.3958081270598939
1102	62-63	0.34059935477694836
1102	64-65	0.35554870602238964
1102	66-67	0.3291327536832753
1102	68-69	0.4503865964220424
1102	70-71	0.410370231310921
1102	72-73	0.4061607521650643
1102	74-75	0.4101473742109434
1102	76-77	0.40084571649245504
1102	78-79	0.3325359411852773
1102	80-81	0.3202736592956512
1102	82-83	0.3145094019366752
1102	84-85	0.4331966914611698
1102	86-87	0.3762969292765419
1102	88-89	0.3100929860119166
1102	90-91	0.3380308297896448
1102	92-93	0.3302522204941809
1102	94-95	0.3259268138812743
1102	96-97	0.4225904942813088
1102	98-99	0.34120007183671675
1102	100	0.37796733445994946
1103	1	0.09847348332835892
1103	2	0.10770484989535589
1103	3	0.15951277520169072
1103	4	-0.020826259969247474
1103	5	0.16251364848647398
1103	6	0.14775090529760604
1103	7	0.07436661846480774
1103	8	0.11738170400384718
1103	9	0.17187007466558413
1103	10-11	0.16779723773751698
1103	12-13	0.1889777105053696
1103	14-15	0.27020830185652756
1103	16-17	0.22843151314584986
1103	18-19	0.15635953848922668
1103	20-21	0.17270831056550406
1103	22-23	0.16369496124645622
1103	24-25	0.2117210331376782
1103	26-27	0.1862570525310474
1103	28-29	0.2933908506355465
1103	30-31	0.2676878747386482
1103	32-33	0.25092583142824054
1103	34-35	0.2969406708842115
1103	36-37	0.3791741747683446
1103	38-39	0.33338511205558774
1103	40-41	0.30314382362912795
1103	42-43	0.3751673332756127
1103	44-45	0.3144998966637118
1103	46-47	0.3072961544779673
1103	48-49	0.23690449413742698
1103	50-51	0.27086598236603976
1103	52-53	0.28409638862150643
1103	54-55	0.32397227149840546
1103	56-57	0.34075749650681786
1103	58-59	0.2947510088103229
1103	60-61	0.2983681692312672
1103	62-63	0.302818460779541
1103	64-65	0.23399231186031244
1103	66-67	0.2586587298867258
1103	68-69	0.2811521206665262
1103	70-71	0.2590308988609351
1103	72-73	0.20313301710139342
1103	74-75	0.30396691657880837
1103	76-77	0.2844094224275082
1103	78-79	0.2501005887606098
1103	80-81	0.2923049579250403
1103	82-83	0.270540850547917
1103	84-85	0.32074645664582846
1103	86-87	0.2464330448993124
1103	88-89	0.2522968007972395
1103	90-91	0.27261487315393396
1103	92-93	0.2666175740870429
1103	94-95	0.24970212026686767
1103	96-97	0.2552298858339128
1103	98-99	0.13406063621118847
1103	100	0.24459754545306822
1104	1	0.09881786767314793
1104	2	0.11118716417297492
1104	3	0.186551171387638
1104	4	0.15512115463162246
1104	5	0.1423246907413045
1104	6	0.16298053575304294
1104	7	0.24763567759210048
1104	8	0.2673038617266883
1104	9	0.31921112880041846
1104	10-11	0.2959912709637109
1104	12-13	0.21990566323977134
1104	14-15	0.2984173898525171
1104	16-17	0.288585288242416
1104	18-19	0.16172084518098018
1104	20-21	0.18157939481506702
1104	22-23	0.18423427039189022
1104	24-25	0.18903729026543914
1104	26-27	0.2527475440466773
1104	28-29	0.307756518170045
1104	30-31	0.25378930534204613
1104	32-33	0.2451205165089192
1104	34-35	0.3082361957186066
1104	36-37	0.27049419195643054
1104	38-39	0.2099186916417608
1104	40-41	0.2495737120985666
1104	42-43	0.28283530347828645
1104	44-45	0.2237592546189262
1104	46-47	0.1685506993543413
1104	48-49	0.2069425372664071
1104	50-51	0.22066670124426224
1104	52-53	0.19708456325179213
1104	54-55	0.23898498280907177
1104	56-57	0.25686692296456215
1104	58-59	0.22286251877111596
1104	60-61	0.1924862165646175
1104	62-63	0.14018792032219807
1104	64-65	0.08524755425211339
1104	66-67	0.15426183278523098
1104	68-69	0.10831365315758745
1104	70-71	0.07922376298900957
1104	72-73	0.12791594785367266
1104	74-75	0.07501507471926772
1104	76-77	0.12220977008817613
1104	78-79	0.15752920961990924
1104	80-81	0.12403328923019075
1104	82-83	0.1499497355009396
1104	84-85	0.10765011894280008
1104	86-87	0.18594967337805457
1104	88-89	0.19879940580610622
1104	90-91	0.1716722340505683
1104	92-93	0.19365797905812343
1104	94-95	0.1711348643985673
1104	96-97	0.1594830695545042
1104	98-99	0.13857269377718495
1104	100	0.1299989477926431
1105	1	0.04208712175283935
1105	2	0.1282119321300641
1105	3	0.07261783875220473
1105	4	0.12563933513748538
1105	5	0.05406766554083475
1105	6	0.05879814335560951
1105	7	0.03886565104614448
1105	8	0.1321342497339586
1105	9	0.11400005322104079
1105	10-11	0.19153053313171142
1105	12-13	0.0685856453992173
1105	14-15	0.13856377551483234
1105	16-17	0.16158070523644597
1105	18-19	0.1310306352817321
1105	20-21	0.10361677733352792
1105	22-23	0.11671726869598587
1105	24-25	0.12882807009271602
1105	26-27	0.14751323800457783
1105	28-29	0.17854628089838087
1105	30-31	0.2380589486852287
1105	32-33	0.2356731632251723
1105	34-35	0.13506394345603212
1105	36-37	0.17396056543119442
1105	38-39	0.18615188967412166
1105	40-41	0.15686594925341524
1105	42-43	0.1875173333344975
1105	44-45	0.12256989349589986
1105	46-47	0.12915650409118484
1105	48-49	0.16648485789390577
1105	50-51	0.12112158579857635
1105	52-53	0.11041331155892209
1105	54-55	0.16618933539749037
1105	56-57	0.12565967858643035
1105	58-59	0.11248400479344411
1105	60-61	0.04205220016982025
1105	62-63	0.0802517424544007
1105	64-65	0.1265588653179961
1105	66-67	0.07977927360781223
1105	68-69	0.1158849382395104
1105	70-71	0.10302021427239794
1105	72-73	0.11872930882022104
1105	74-75	0.1429278482918903
1105	76-77	0.05923662072890323
1105	78-79	0.043625044197533214
1105	80-81	0.010906135608578893
1105	82-83	0.021704564517023073
1105	84-85	0.08678529971203375
1105	86-87	0.010685026547214704
1105	88-89	0.017177339607620468
1105	90-91	0.030226781353384524
1105	92-93	0.030109288614333707
1105	94-95	0.022055349961384252
1105	96-97	-0.001759758134831202
1105	98-99	-0.03273337338880822
1105	100	0.042135456134946736
1106	1	0.04004936676737714
1106	2	0.0748917320434046
1106	3	0.08421855895207742
1106	4	0.16283223704070338
1106	5	0.0700436646268443
1106	6	0.11672292870758838
1106	7	0.025483966670883262
1106	8	-0.0034283942898483133
1106	9	0.06985695849567719
1106	10-11	0.07110797078516384
1106	12-13	0.1581910201682888
1106	14-15	0.16965769358751714
1106	16-17	0.11621738261665371
1106	18-19	0.10854048925857285
1106	20-21	0.08055787873706777
1106	22-23	0.11803389418989951
1106	24-25	0.14023269426737528
1106	26-27	0.1750016433471231
1106	28-29	0.1938881845239564
1106	30-31	0.24683894443861476
1106	32-33	0.22893691783554715
1106	34-35	0.21395099645679494
1106	36-37	0.21400565067605015
1106	38-39	0.269546591430327
1106	40-41	0.2970079171171136
1106	42-43	0.18369632380440493
1106	44-45	0.19173500271148214
1106	46-47	0.12241001940378027
1106	48-49	0.16215019937987307
1106	50-51	0.1620281193328168
1106	52-53	0.16967769346101846
1106	54-55	0.23058441385207118
1106	56-57	0.1472718048251167
1106	58-59	0.20346690193269268
1106	60-61	0.28207279193655665
1106	62-63	0.21016754848114516
1106	64-65	0.1976593365412569
1106	66-67	0.1377534366260491
1106	68-69	0.16438889599755413
1106	70-71	0.20394277500221136
1106	72-73	0.1692079016820287
1106	74-75	0.13557347266270625
1106	76-77	0.15737880792913472
1106	78-79	0.27503822626948704
1106	80-81	0.2608866674700465
1106	82-83	0.12642415326371292
1106	84-85	0.24133072608717043
1106	86-87	0.1664768853354346
1106	88-89	0.13090831876472464
1106	90-91	0.1942605338512351
1106	92-93	0.11731449114764558
1106	94-95	0.10520087301704706
1106	96-97	0.09451685595573878
1106	98-99	0.044312155379309104
1106	100	0.005319522063089721
1107	1	0.010743873950573857
1107	2	0.03232883226636929
1107	3	0.13120774638855082
1107	4	0.07343757859892719
1107	5	0.053992912637845336
1107	6	0.04548336248987539
1107	7	0.1307668699824518
1107	8	0.05933858395882652
1107	9	0.1568908201548922
1107	10-11	0.23896170117417626
1107	12-13	0.1766754432261166
1107	14-15	0.21955042619297416
1107	16-17	0.12025111401625566
1107	18-19	0.12989716216906544
1107	20-21	0.15104362775142022
1107	22-23	0.14058670836885057
1107	24-25	0.13778366119742458
1107	26-27	0.12694143242811862
1107	28-29	0.10101276324329689
1107	30-31	0.07747637353536163
1107	32-33	0.09223948400070014
1107	34-35	0.07354668636172335
1107	36-37	0.062411963671358706
1107	38-39	0.09831664868107737
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2216	46-47	-0.6489955969720356
2216	48-49	-0.7020982128578126
2216	50-51	-0.6212302613916236
2216	52-53	-0.6914369458478191
2216	54-55	-0.6908418730364616
2216	56-57	-0.7571502964312558
2216	58-59	-0.719931517115306
2216	60-61	-0.7709002369555193
2216	62-63	-0.7591213154805772
2216	64-65	-0.7800062673638024
2216	66-67	-0.7663637892346955
2216	68-69	-0.7694464208347611
2216	70-71	-0.793863329721809
2216	72-73	-0.679373577049688
2216	74-75	-0.6642733367400098
2216	76-77	-0.6503967662324897
2216	78-79	-0.6648620380177803
2216	80-81	-0.6090463068769125
2216	82-83	-0.6010384611220019
2216	84-85	-0.5611046885829722
2216	86-87	-0.6617062733985506
2216	88-89	-0.6350262118352106
2216	90-91	-0.6456925702897252
2216	92-93	-0.700462790237971
2216	94-95	-0.6193999411508386
2216	96-97	-0.6473062402426137
2216	98-99	-0.5717526757184501
2216	100	-0.5769822656624761
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	51.0
1	37.0
2	43.0
3	31.0
4	40.0
5	44.0
6	99.0
7	456.0
8	1391.0
9	1842.0
10	1961.0
11	2390.0
12	3149.0
13	4114.0
14	5414.0
15	6491.0
16	6706.0
17	7411.0
18	8123.0
19	9268.0
20	10638.0
21	12031.0
22	14193.0
23	17239.0
24	22913.0
25	27690.0
26	30039.0
27	27044.0
28	27403.0
29	33976.0
30	39151.0
31	43557.0
32	55186.0
33	75387.0
34	117903.0
35	190120.0
36	338916.0
37	666862.0
38	662971.0
39	237579.0
40	298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.440777243870784	20.620259216664298	13.01765338955048	22.921310149914436
2	14.500886371721124	22.413691306472064	38.615990706750445	24.469431615056365
3	19.27863698258588	27.802330001070164	30.254772369154242	22.664260647189717
4	12.026239927732076	18.178976765331473	40.25538060958347	29.53940269735298
5	12.164271580682945	40.274346883632845	35.06432449866303	12.497057037021182
6	28.08073740636839	38.67648871401885	18.765882039484513	14.476891840128244
7	24.845199267775286	34.10184211118643	23.68834030663847	17.364618314399817
8	25.39985763979473	33.4668621382739	21.307130888363844	19.826149333567525
9	25.56653029324038	16.90714368214155	22.034154735068142	35.49217128954992
10-11	24.467974011530014	26.663384958732028	27.739042225913117	21.129598803824837
12-13	26.105018373538268	25.19085251114248	26.941472388585808	21.762656726733447
14-15	23.322757273900752	26.111408785731104	25.925608160319186	24.64022578004896
16-17	21.512292245524232	28.81558891759639	27.314112571333364	22.35800626554601
18-19	21.236107449195103	29.936598472924135	27.75875426506868	21.06853981281208
20-21	23.127915496070912	27.980773293387166	26.174727087519635	22.716584123022287
22-23	22.837506794027234	27.863647886185795	26.002481088343206	23.29636423144377
24-25	23.395125072368952	28.157915419928116	26.0093185750484	22.43764093265453
26-27	23.190140357836377	27.236172931186374	26.9401807170565	22.63350599392075
28-29	21.973824067341486	29.06524093928119	26.680783473557373	22.280151519819956
30-31	22.79295783457417	27.81101455072606	26.460354100940116	22.935673513759657
32-33	22.718562251121167	28.484801854531323	26.146507827032924	22.650128067314586
34-35	23.184337183781803	28.18293950881181	26.806409562126422	21.82631374527996
36-37	23.133238021147793	27.28764007127009	27.356227202206007	22.22289470537611
38-39	23.20247025752516	27.736073340106586	26.99962024994123	22.061836152427023
40-41	22.473967026099125	27.763172777977985	27.079274979393386	22.6835852165295
42-43	22.56966178511323	27.80614278543801	26.008315125926362	23.615880303522395
44-45	22.902509018911804	27.636245128385124	26.455579195078844	23.00566665762423
46-47	23.18142493432062	27.998552433145075	26.819534696944757	22.00048793558955
48-49	23.475490081188223	27.352992252857405	26.94379386130439	22.227723804649983
50-51	21.78308287522026	27.772337672212256	27.564184428314007	22.88039502425348
52-53	22.083819839331685	28.668116819017552	25.794515737142632	23.45354760450813
54-55	21.955726299307578	27.975360190285446	26.076898175951293	23.992015334455687
56-57	23.522115074055275	26.69282517417986	26.159590273612327	23.62546947815254
58-59	23.531289910600258	26.97262640356122	26.851851851851855	22.644231833986666
60-61	22.820325517151772	27.822842897039163	27.252704337792515	22.104127248016546
62-63	22.327094109164246	27.522635588842736	26.773803194164365	23.376467107828653
64-65	21.208434592340833	28.95947326614558	26.484465662492283	23.3476264790213
66-67	23.21468712800022	27.08167615602536	25.94657045041387	23.75706626556055
68-69	22.401366647330683	27.22927793854184	26.424087749802844	23.945267664324632
70-71	23.173981580307785	27.395439427280866	25.949208808865933	23.481370183545415
72-73	23.500834930356028	26.69996176004205	27.446176151849542	22.353027157752376
74-75	22.88105813379636	28.647485721520464	26.330520270771572	22.140935873911605
76-77	22.70690399140983	27.48614598786406	26.42844988249051	23.378500138235598
78-79	22.875090341188866	27.785129342006087	26.59332696977012	22.746453347034926
80-81	22.896605388580877	27.712456444497846	26.7715154813867	22.61942268553458
82-83	22.901962579254885	26.675763037629295	27.998231172339256	22.42404321077656
84-85	22.97532377988283	28.093662890957194	26.2961899390532	22.634823390106774
86-87	22.29783274443127	28.30821410419525	26.55737928799507	22.836573863378412
88-89	22.82677118418389	27.28983476963104	26.091977801961626	23.791416244223452
90-91	23.19958719576034	26.69447115823812	26.45817173564362	23.647769910357923
92-93	23.658801352557933	27.568259993496326	26.58001127254648	22.192927381399265
94-95	22.153090196622703	27.542884331162416	27.32342419092645	22.980601281288436
96-97	21.922397488915827	27.5857537903227	27.59826639520343	22.89358232555805
98-99	22.553950096564375	28.362078113336032	26.01339997951525	23.070571810584344
100	22.76743417835281	26.837774345289834	25.742589240073006	24.652202236284353
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5810.0
1	3852.5
2	1889.0
3	1765.5
4	1509.5
5	1137.0
6	824.0
7	761.0
8	818.5
9	852.5
10	703.0
11	532.5
12	513.0
13	527.0
14	535.5
15	628.5
16	697.5
17	766.0
18	938.0
19	1097.0
20	1340.0
21	1712.5
22	2501.0
23	3515.0
24	4484.5
25	5951.0
26	8193.0
27	10640.5
28	13880.0
29	18858.0
30	25105.0
31	31957.0
32	39982.0
33	47657.5
34	52887.5
35	57782.5
36	63453.5
37	71643.0
38	75846.5
39	75189.5
40	72233.0
41	66390.0
42	65053.0
43	70864.5
44	79621.5
45	93282.5
46	106899.0
47	130830.0
48	176489.0
49	179994.5
50	158277.5
51	142519.0
52	116040.5
53	103172.0
54	93568.0
55	78976.0
56	69413.0
57	57835.0
58	45146.5
59	40839.5
60	33812.0
61	24635.0
62	18363.0
63	14621.0
64	10902.5
65	6532.0
66	4375.0
67	3350.5
68	2530.5
69	1910.5
70	1524.5
71	1161.0
72	843.5
73	641.0
74	436.5
75	280.5
76	237.5
77	196.0
78	141.0
79	86.0
80	58.0
81	45.0
82	33.0
83	25.0
84	18.0
85	17.5
86	14.0
87	12.0
88	11.5
89	9.5
90	7.0
91	8.5
92	8.5
93	5.5
94	5.0
95	5.0
96	4.0
97	3.0
98	2.5
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059479948947607095
2	0.008597287906198792
3	0.010589792399480916
4	0.008892473757055404
5	0.011180164101194138
6	0.012360907504620579
7	0.0022138938814245815
8	0.0047967700764199265
9	0.007232053345986968
10-11	0.0068630710324162035
12-13	0.005368692662454611
14-15	0.009704234846911084
16-17	0.009501294574447164
18-19	0.0076194847752362686
20-21	0.006512537834523978
22-23	0.002066300955996276
24-25	0.0031732478967085673
26-27	0.004409338647170626
28-29	0.00547938735652584
30-31	0.008246754708306566
32-33	0.009372150764697395
34-35	0.01105102029144437
36-37	0.01715767758104051
38-39	0.017711151051396652
40-41	0.017286821390790276
42-43	0.02350417337445764
44-45	0.02625309161055983
46-47	0.028891315152590788
48-49	0.030828472298837298
50-51	0.031547987810300285
52-53	0.029020458962340557
54-55	0.03556989502822162
56-57	0.0380789747605028
58-59	0.036049572035863606
60-61	0.036566147274862675
62-63	0.032193706859049126
64-65	0.03442604985615225
66-67	0.036234063192648984
68-69	0.03857710088382334
70-71	0.03857710088382334
72-73	0.03501642155786547
74-75	0.03726721367064712
76-77	0.037414806596075434
78-79	0.036270961424006064
80-81	0.04108618061610453
82-83	0.03756239952150373
84-85	0.033540492303582414
86-87	0.032396647131513044
88-89	0.030201202365767
90-91	0.03256268917261989
92-93	0.03394637284851025
94-95	0.03723031543929005
96-97	0.03280252767644088
98-99	0.03042259175390946
100	0.027747469980521425
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2710157.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.00710211240701
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.0635271448362	36.43801010576897
2	13.962850437946074	14.523347770130565
3	6.025407208635795	9.400919039050654
4	3.236426372973723	6.732686274341258
5	1.9494725658768426	5.069320939944654
6	1.2550670060996016	3.916343876648097
7	0.8162186144720809	2.9714415380227814
8	0.5741061048887462	2.388607585624422
9	0.42999321644044486	2.012643100355449
>10	1.6543594588440083	13.410905567023645
>50	0.024764918489417545	0.8343733885887901
>100	0.007187396287748245	0.5976716373187978
>500	1.2391084185256552E-4	0.05194060506878918
>1k	2.4782168370513104E-4	0.3998653281040159
>5k	1.2391084185256552E-4	0.406245129471026
>10k+	1.2391084185256552E-4	0.8456781145381145
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	13123	0.48421549009891307	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6304	0.23260645047500939	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	4986	0.18397458154638274	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	3.6898231357076365E-5	0.0
16-17	0.0	0.0	0.0	3.6898231357076365E-5	0.0
18-19	0.0	0.0	0.0	7.379646271415273E-5	0.0
20-21	0.0	0.0	0.0	2.9518585085661086E-4	0.0
22-23	0.0	0.0	0.0	7.564137428200654E-4	0.0
24-25	0.0	0.0	0.0	0.0018080133364967417	0.0
26-27	0.0	0.0	0.0	0.0040588054492784	0.0
28-29	0.0	0.0	0.0	0.01136465525797952	0.0
30-31	0.0	0.0	0.0	0.025625821677489533	0.0
32-33	0.0	0.0	0.0	0.05442489125168763	0.0
34-35	0.0	0.0	0.0	0.09897950561535734	0.0
36-37	0.0	0.0	0.0	0.16345916491184828	0.0
38-39	0.0	0.0	0.0	0.25496677867739764	0.0
40-41	0.0	0.0	0.0	0.35914893491410277	0.0
42-43	0.0	0.0	0.0	0.4529995863708265	0.0
44-45	0.0	0.0	0.0	0.5603365413885616	0.0
46-47	0.0	0.0	0.0	0.6881520148094742	0.0
48-49	0.0	0.0	0.0	0.8255794774989051	0.0
50-51	0.0	0.0	0.0	0.964003192434977	0.0
52-53	0.0	0.0	0.0	1.0965231903539168	0.0
54-55	0.0	0.0	0.0	1.2339322039276692	0.0
56-57	0.0	0.0	0.0	1.4039408049053983	0.0
58-59	0.0	0.0	0.0	1.5860151275368919	0.0
60-61	0.0	0.0	0.0	1.7667980120708875	0.0
62-63	0.0	0.0	0.0	1.9440571155102822	0.0
64-65	0.0	0.0	0.0	2.132422586588157	0.0
66-67	0.0	0.0	0.0	2.338831292799642	0.0
68-69	0.0	0.0	0.0	2.5721203605547576	0.0
70-71	0.0	0.0	0.0	2.827972696784725	0.0
72-73	0.0	0.0	0.0	3.0631804725704086	0.0
74-75	0.0	0.0	0.0	3.297373546993772	0.0
76-77	0.0	0.0	0.0	3.5480232326023917	0.0
78-79	0.0	0.0	0.0	3.8327484348692713	0.0
80-81	0.0	0.0	0.0	4.132472768182803	0.0
82-83	0.0	0.0	0.0	4.437916327356681	0.0
84-85	0.0	0.0	0.0	4.738304828834639	0.0
86-87	0.0	0.0	0.0	5.044154268553445	0.0
88	0.0	0.0	0.0	5.291427765992893	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1535	0.0	28.793066	1
GTACCCG	260	0.0	27.126036	1
TATTCTC	1240	0.0	26.907953	5
GTGATCG	1310	0.0	26.186625	8
AGGGCTA	2510	0.0	25.275747	5
CTAACGC	835	0.0	24.763393	3
TGATCGC	1395	0.0	24.591022	9
GCTATTG	2515	0.0	24.477173	8
TAACGCC	845	0.0	24.470337	4
CGTTAAC	570	0.0	23.921675	1
GGGCACC	1840	0.0	23.756872	94
CGCCTAC	875	0.0	23.630045	7
GGTGATC	1480	0.0	23.495783	7
GGGCTAT	2720	0.0	23.32388	6
TCTAACG	880	0.0	22.96306	2
CATGGGG	7705	0.0	22.749935	4
TTTAGGG	2920	0.0	22.531452	2
TAAGGTG	1800	0.0	22.452768	4
ATTTAGG	2875	0.0	22.40532	1
CTATTGA	2740	0.0	22.295681	9
>>END_MODULE