FastQCFastQC Report
Mon 16 Jan 2017
ERR1146541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2710157
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT261050.9632283295764784No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155270.5729188382813247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146630.5410387663888107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82860.3057387450247347No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC74470.27478112891614764No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA53070.19581891381200428No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGC650.006158238728.9205639
GTACTAG16600.026.0878011
GAGTACT141150.023.92246812-13
AGGGCTA26250.022.7467235
TAGGCAT25000.022.5676145
GGGCACC18650.022.42700694
GGGCTAT27200.022.2889146
GCTATTG26400.022.2518528
AGTACTT143650.021.96850412-13
GTACTTT154650.021.84937714-15
CTGTGCG20550.021.7256059
ATTTAGG28900.021.499771
GTGATCG11900.020.9309548
CTATTGA27200.020.906279
CATGGGG77850.020.7147624
GGCTATT28750.020.4330067
TGATCGC12400.020.0869649
CTAGGCA29050.020.0687334
TACCTGG23700.019.8613782
ACTTTTT170350.019.84946316-17
Produced by FastQC (version 0.11.5)