##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146541_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2710157
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54894236754549	34.0	31.0	34.0	31.0	34.0
2	32.61631890698583	34.0	31.0	34.0	31.0	34.0
3	32.74697148541579	34.0	31.0	34.0	31.0	34.0
4	36.18846288240866	37.0	37.0	37.0	35.0	37.0
5	36.22496335083171	37.0	37.0	37.0	35.0	37.0
6	36.31784505473299	37.0	37.0	37.0	35.0	37.0
7	36.30467976578478	37.0	37.0	37.0	35.0	37.0
8	36.3241967162788	37.0	37.0	37.0	35.0	37.0
9	38.134933511232006	39.0	39.0	39.0	37.0	39.0
10-11	38.102778363024726	39.0	38.5	39.0	37.0	39.0
12-13	38.07200394663482	39.0	38.0	39.0	36.0	39.0
14-15	39.47359119785311	41.0	39.0	41.0	36.5	41.0
16-17	39.47905970023139	41.0	39.0	41.0	36.5	41.0
18-19	39.548698470236225	41.0	39.5	41.0	37.0	41.0
20-21	39.579126781216	41.0	40.0	41.0	37.0	41.0
22-23	39.531747791733096	41.0	39.5	41.0	36.5	41.0
24-25	39.496767161459644	41.0	39.0	41.0	36.0	41.0
26-27	39.389708603597505	41.0	39.0	41.0	36.0	41.0
28-29	39.31282910916231	41.0	39.0	41.0	36.0	41.0
30-31	39.20180030160614	41.0	39.0	41.0	35.0	41.0
32-33	39.02238043773848	41.0	38.5	41.0	35.0	41.0
34-35	38.91846062792672	40.5	38.0	41.0	35.0	41.0
36-37	38.82933461050411	40.5	38.0	41.0	35.0	41.0
38-39	38.71170507834048	40.0	38.0	41.0	35.0	41.0
40-41	38.626445073108314	40.0	38.0	41.0	35.0	41.0
42-43	38.514862238608316	40.0	38.0	41.0	35.0	41.0
44-45	38.31218191418431	40.0	38.0	41.0	34.5	41.0
46-47	38.00287935348395	40.0	37.5	41.0	33.5	41.0
48-49	37.69315375456108	40.0	37.0	41.0	33.0	41.0
50-51	37.4721687710343	40.0	37.0	41.0	33.0	41.0
52-53	37.35836632342702	40.0	36.5	41.0	33.0	41.0
54-55	37.215215945054105	40.0	36.0	41.0	33.0	41.0
56-57	36.9540050631753	39.5	35.0	41.0	32.0	41.0
58-59	36.703207969132414	39.0	35.0	41.0	32.0	41.0
60-61	36.451080324866794	39.0	35.0	41.0	31.0	41.0
62-63	36.173365048593126	38.0	35.0	41.0	31.0	41.0
64-65	35.89495995988425	37.0	35.0	40.0	31.0	41.0
66-67	35.609848986608526	37.0	35.0	40.0	31.0	41.0
68-69	35.292042859509614	36.0	35.0	39.0	30.0	41.0
70-71	35.00481540368325	36.0	35.0	39.0	30.0	41.0
72-73	34.69734723855481	35.0	35.0	39.0	30.0	41.0
74-75	34.41137801241773	35.0	35.0	37.0	30.0	40.0
76-77	34.12311224036098	35.0	34.0	37.0	29.0	39.0
78-79	33.859685804180344	35.0	34.0	36.5	29.0	39.0
80-81	33.648771823920164	35.0	34.0	36.0	29.0	39.0
82-83	33.425971262919454	35.0	34.0	36.0	29.0	37.0
84-85	33.24875385448149	35.0	34.0	35.5	29.0	37.0
86-87	33.11568997663235	35.0	34.0	35.0	29.0	37.0
88-89	32.986636936531724	35.0	34.0	35.0	29.0	36.0
90-91	32.887975124688346	35.0	34.0	35.0	29.0	36.0
92-93	32.77722748165512	35.0	34.0	35.0	29.0	36.0
94-95	32.716912156749586	35.0	34.0	35.0	28.5	36.0
96-97	32.67144025235439	35.0	34.0	35.0	28.5	35.5
98-99	32.655861265601956	35.0	34.0	35.0	29.0	35.0
100	32.066355565378686	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2897813790059871
1101	2	0.2604596629455429
1101	3	0.3085532919972991
1101	4	0.18453324337782107
1101	5	0.20968948046151326
1101	6	0.17935030933059437
1101	7	0.21305064478532643
1101	8	0.1553650015980068
1101	9	0.20515737943890855
1101	10-11	0.23064278661994564
1101	12-13	0.23799472041039849
1101	14-15	0.33636979426303526
1101	16-17	0.34337438517160024
1101	18-19	0.34830345893175263
1101	20-21	0.34609200496811354
1101	22-23	0.37530347363009753
1101	24-25	0.3366604985781052
1101	26-27	0.3103249255475191
1101	28-29	0.38844653788550687
1101	30-31	0.37950527155543057
1101	32-33	0.34196336177828357
1101	34-35	0.44958852968577645
1101	36-37	0.39543266083303763
1101	38-39	0.40828428823891727
1101	40-41	0.417793073202823
1101	42-43	0.3743610650662319
1101	44-45	0.396061041147334
1101	46-47	0.3403077896155722
1101	48-49	0.3615005481429492
1101	50-51	0.32113193076101254
1101	52-53	0.41702440885562453
1101	54-55	0.361175344715555
1101	56-57	0.39165428112455203
1101	58-59	0.3910827790777134
1101	60-61	0.3935487887146536
1101	62-63	0.39091375967083053
1101	64-65	0.3829688172452137
1101	66-67	0.2902298797933085
1101	68-69	0.3323762298007935
1101	70-71	0.30473120798478703
1101	72-73	0.2831484234853505
1101	74-75	0.29451134861689354
1101	76-77	0.30065651004034777
1101	78-79	0.36582268280154295
1101	80-81	0.33418011711957263
1101	82-83	0.3218879608954026
1101	84-85	0.3258024266377433
1101	86-87	0.30517299887225136
1101	88-89	0.2887285381292628
1101	90-91	0.3589004688565751
1101	92-93	0.2768468183073054
1101	94-95	0.29505445269019503
1101	96-97	0.23964533192744852
1101	98-99	0.307086971712053
1101	100	0.487080785127759
1102	1	0.22781873758008686
1102	2	0.2173316202819393
1102	3	0.22532541107725734
1102	4	0.14136257101522887
1102	5	0.14665012751552808
1102	6	0.11484359727202786
1102	7	0.160310976130269
1102	8	0.1716282733118959
1102	9	0.15382640212355625
1102	10-11	0.17997863585340923
1102	12-13	0.18459744917648635
1102	14-15	0.2656858892430023
1102	16-17	0.28895624483030957
1102	18-19	0.24839697835328423
1102	20-21	0.27930477021126876
1102	22-23	0.3341309956961567
1102	24-25	0.2732437107533414
1102	26-27	0.3131084454543469
1102	28-29	0.3626059599963085
1102	30-31	0.3525619306634269
1102	32-33	0.35258384909640483
1102	34-35	0.31439562738939486
1102	36-37	0.40327594375528975
1102	38-39	0.39620736930415035
1102	40-41	0.32552516158274614
1102	42-43	0.34555668436190956
1102	44-45	0.3586445471600257
1102	46-47	0.3359523722699933
1102	48-49	0.3040566548099335
1102	50-51	0.2340192211608425
1102	52-53	0.2081725388328337
1102	54-55	0.2469116302511054
1102	56-57	0.3060725012407204
1102	58-59	0.31246567820304705
1102	60-61	0.316498391601975
1102	62-63	0.32579639587147113
1102	64-65	0.2797217637634688
1102	66-67	0.2869892332312389
1102	68-69	0.30553518356659737
1102	70-71	0.29755865725627473
1102	72-73	0.24066069884428742
1102	74-75	0.2151144898855648
1102	76-77	0.2762912687512653
1102	78-79	0.25673702253481423
1102	80-81	0.2934397946429286
1102	82-83	0.275570211251555
1102	84-85	0.26328302306130524
1102	86-87	0.29540880343178344
1102	88-89	0.24134974109449558
1102	90-91	0.1512190873817758
1102	92-93	0.22129388036484698
1102	94-95	0.22875293058557133
1102	96-97	0.24931063976207213
1102	98-99	0.245335152013908
1102	100	0.40924236182498674
1103	1	0.1574471567469189
1103	2	0.16447365065657493
1103	3	0.16057247821910892
1103	4	0.11064716938327734
1103	5	0.09279159616715305
1103	6	0.12019142717085884
1103	7	0.0723915182768593
1103	8	0.12695219197541263
1103	9	0.1524571087499922
1103	10-11	0.14608112182629185
1103	12-13	0.1657392396116606
1103	14-15	0.19625815430536875
1103	16-17	0.24943362352559006
1103	18-19	0.22814229908089345
1103	20-21	0.24985015354684492
1103	22-23	0.2184175527295551
1103	24-25	0.215239488732621
1103	26-27	0.25463501811925937
1103	28-29	0.24205942265117386
1103	30-31	0.21655855796223022
1103	32-33	0.2437505086342
1103	34-35	0.23166113836079916
1103	36-37	0.2222941234623903
1103	38-39	0.2904787871502137
1103	40-41	0.28955098177829797
1103	42-43	0.216765162369839
1103	44-45	0.19462702165425583
1103	46-47	0.24561426007678477
1103	48-49	0.17365420638765983
1103	50-51	0.21986822834633557
1103	52-53	0.31302486145230546
1103	54-55	0.30979810036749456
1103	56-57	0.2549268375871563
1103	58-59	0.16792708548499746
1103	60-61	0.117371051256832
1103	62-63	0.18020497621996157
1103	64-65	0.2602943084663707
1103	66-67	0.18936069527625676
1103	68-69	0.16901093113043686
1103	70-71	0.20905652304756472
1103	72-73	0.18107103312973294
1103	74-75	0.20032887059323912
1103	76-77	0.18169411300275584
1103	78-79	0.13958835404520897
1103	80-81	0.20247134904494857
1103	82-83	0.20209395088210869
1103	84-85	0.23880876499688242
1103	86-87	0.14824387404180328
1103	88-89	0.12520458827724212
1103	90-91	0.173882062478242
1103	92-93	0.15202853386934123
1103	94-95	0.1416140243077706
1103	96-97	0.0865542536977486
1103	98-99	0.05633595871778141
1103	100	0.12020897361121285
1104	1	0.134623725476537
1104	2	0.16950673223452384
1104	3	0.1875988661691821
1104	4	0.13506191428128034
1104	5	0.13807094739033943
1104	6	0.12226803660247043
1104	7	0.12192059899970786
1104	8	0.11711737267230404
1104	9	0.14916195987714786
1104	10-11	0.1335219896036719
1104	12-13	0.14718266069579755
1104	14-15	0.2202235539820805
1104	16-17	0.25720680662946904
1104	18-19	0.20568078269717205
1104	20-21	0.2063801543822379
1104	22-23	0.2617987502486585
1104	24-25	0.22854969163400796
1104	26-27	0.20930759689013456
1104	28-29	0.2483001102457294
1104	30-31	0.27896799121722893
1104	32-33	0.2327182754900079
1104	34-35	0.25853657092599036
1104	36-37	0.3212949492880455
1104	38-39	0.32460371031047686
1104	40-41	0.3256679378446776
1104	42-43	0.27036003162128
1104	44-45	0.3318991700114111
1104	46-47	0.3579904816646149
1104	48-49	0.37236923619959583
1104	50-51	0.27158017656326905
1104	52-53	0.25245713578030404
1104	54-55	0.21826295044504462
1104	56-57	0.31378124002509367
1104	58-59	0.32834951891545217
1104	60-61	0.22189573970797483
1104	62-63	0.22650925261219612
1104	64-65	0.3131661633185274
1104	66-67	0.2200656061480899
1104	68-69	0.2329229875549217
1104	70-71	0.2509741241637613
1104	72-73	0.2521401093205853
1104	74-75	0.2866902127908233
1104	76-77	0.2042505463372848
1104	78-79	0.2173552091052784
1104	80-81	0.2584021166729116
1104	82-83	0.19953252745845163
1104	84-85	0.16963509988834602
1104	86-87	0.24258350297693454
1104	88-89	0.1628229444520457
1104	90-91	0.2339313955726965
1104	92-93	0.1811352004342126
1104	94-95	0.13045711306026675
1104	96-97	0.21645905284851352
1104	98-99	0.1868569582685069
1104	100	0.20415388071453577
1105	1	0.15030287593996405
1105	2	0.14572984054619553
1105	3	0.1162362345827006
1105	4	0.054491327835030745
1105	5	0.05724374456670489
1105	6	0.05523048603678404
1105	7	0.0947361365910453
1105	8	0.06445336960007353
1105	9	0.11303325278290544
1105	10-11	0.08816907390167472
1105	12-13	0.07254603527140802
1105	14-15	0.11197069965864159
1105	16-17	0.1330317187765857
1105	18-19	0.14549012347821133
1105	20-21	0.09782223236055643
1105	22-23	0.14472978724879937
1105	24-25	0.1557795267934452
1105	26-27	0.1397203429140248
1105	28-29	0.1525675662201067
1105	30-31	0.14875983580274266
1105	32-33	0.1278571169338676
1105	34-35	0.13145061587228213
1105	36-37	0.15924652799530037
1105	38-39	0.20655735296823963
1105	40-41	0.14638527497385923
1105	42-43	0.08230048257186695
1105	44-45	0.1515880787843571
1105	46-47	0.21644791971028354
1105	48-49	0.17159450405584664
1105	50-51	0.08999330386151883
1105	52-53	0.14865219535718666
1105	54-55	0.14016682495288535
1105	56-57	0.1214406534796737
1105	58-59	0.06835546117552838
1105	60-61	-0.0082419391796833
1105	62-63	0.05907130198391286
1105	64-65	0.0644035487571557
1105	66-67	-0.018647233376178463
1105	68-69	0.03631758282556774
1105	70-71	0.016510916588529767
1105	72-73	0.024411256548681592
1105	74-75	7.494224399309246E-4
1105	76-77	-0.007279242650490403
1105	78-79	0.005619527830816651
1105	80-81	-0.07948864822296287
1105	82-83	-0.050451632024426374
1105	84-85	0.03542890125983433
1105	86-87	0.010963530481582495
1105	88-89	0.031047645355528175
1105	90-91	0.0037764987316535326
1105	92-93	-0.02871208458380181
1105	94-95	0.008848877699378477
1105	96-97	0.013296431620346993
1105	98-99	0.06228138081967671
1105	100	0.09796938469642669
1106	1	0.13824300407130607
1106	2	0.11334590568105796
1106	3	0.12023468036507978
1106	4	0.0934199254392496
1106	5	0.06291806699464786
1106	6	0.036774825429624514
1106	7	0.04293269985158332
1106	8	0.050915266478483545
1106	9	0.12219728956677756
1106	10-11	0.0823073453788723
1106	12-13	0.10128336548768146
1106	14-15	0.17700883295920278
1106	16-17	0.1506573899895045
1106	18-19	0.11638572790935342
1106	20-21	0.14464539591555337
1106	22-23	0.2058224900785035
1106	24-25	0.10108375788686175
1106	26-27	0.12717504749412
1106	28-29	0.2151661194612231
1106	30-31	0.20508607815290958
1106	32-33	0.16995403619862515
1106	34-35	0.1648179948707309
1106	36-37	0.1564866330612773
1106	38-39	0.19489977772018108
1106	40-41	0.13950924969680756
1106	42-43	0.09346690834559013
1106	44-45	0.22273610885125095
1106	46-47	0.1295334593428379
1106	48-49	0.03615156550672083
1106	50-51	0.15057060624845775
1106	52-53	0.16939802311279095
1106	54-55	0.1078066695095643
1106	56-57	0.11666173069691155
1106	58-59	0.14019747312209319
1106	60-61	0.1339356280278423
1106	62-63	0.08313115930199189
1106	64-65	0.13240023284140534
1106	66-67	0.09037184998032899
1106	68-69	0.20222937073629765
1106	70-71	0.1849293115408841
1106	72-73	0.18248351473419433
1106	74-75	0.17136502249510954
1106	76-77	0.13184950627977088
1106	78-79	0.10982600650397245
1106	80-81	0.13282238284198655
1106	82-83	0.16143312090670037
1106	84-85	0.12768571524305372
1106	86-87	0.040456345728003384
1106	88-89	0.04216880265485656
1106	90-91	0.09009519364153817
1106	92-93	0.010015297346321006
1106	94-95	0.11768648297552886
1106	96-97	0.06749060966341602
1106	98-99	0.06408666332728785
1106	100	0.05465850237074932
1107	1	0.10207526726986771
1107	2	0.02857013122672214
1107	3	0.04731380025698684
1107	4	0.05546968867399471
1107	5	0.017452129883380962
1107	6	0.04208106374783682
1107	7	0.03750724732638844
1107	8	0.03635285117579912
1107	9	0.030176490686855573
1107	10-11	0.02904055871085376
1107	12-13	0.05280914229075506
1107	14-15	0.055708313875918236
1107	16-17	0.07312429122503517
1107	18-19	0.06567722226134265
1107	20-21	0.03156995884123148
1107	22-23	0.05582867296655536
1107	24-25	0.0779894649083559
1107	26-27	0.10573045569295658
1107	28-29	0.043284815168931345
1107	30-31	0.07208745870167377
1107	32-33	0.07396330265925855
1107	34-35	0.09250042244270418
1107	36-37	0.11922317931270499
1107	38-39	0.10135179279576079
1107	40-41	0.06509374844740279
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2216	44-45	-0.6646118702345802
2216	46-47	-0.6176313608134336
2216	48-49	-0.5274431637667547
2216	50-51	-0.5675342255181093
2216	52-53	-0.6218744591253795
2216	54-55	-0.7001449804598465
2216	56-57	-0.6962235619399593
2216	58-59	-0.61578093978342
2216	60-61	-0.6586102565503253
2216	62-63	-0.6442207935285609
2216	64-65	-0.5841075231475941
2216	66-67	-0.5840428855725364
2216	68-69	-0.6090609972643435
2216	70-71	-0.6217054488107294
2216	72-73	-0.5855430252654443
2216	74-75	-0.5871393925758213
2216	76-77	-0.6039529364194465
2216	78-79	-0.6581352903565687
2216	80-81	-0.6338171354431523
2216	82-83	-0.6119633278913952
2216	84-85	-0.6428743314719227
2216	86-87	-0.6581366318319724
2216	88-89	-0.6288563452630029
2216	90-91	-0.6239996417712206
2216	92-93	-0.60541687819385
2216	94-95	-0.6625424796199297
2216	96-97	-0.6054079623600757
2216	98-99	-0.6530519148601925
2216	100	-0.7924009705272645
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	8.0
8	68.0
9	259.0
10	664.0
11	677.0
12	493.0
13	420.0
14	508.0
15	661.0
16	1087.0
17	2253.0
18	4014.0
19	6420.0
20	9234.0
21	13567.0
22	20469.0
23	24996.0
24	24296.0
25	20249.0
26	15864.0
27	15917.0
28	18376.0
29	22860.0
30	27268.0
31	34891.0
32	45201.0
33	59904.0
34	92137.0
35	158968.0
36	288927.0
37	700981.0
38	790100.0
39	307691.0
40	729.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.17772595354638	20.06937230974736	13.634832279526126	22.118069457180127
2	14.883179451343423	22.25850844658107	38.733939898488856	24.124372203586645
3	19.188619957286466	27.694649886279272	31.29312495110968	21.823605205324583
4	11.961557946642944	18.167840460903186	39.86916625125408	30.001435341199787
5	11.604626568997116	39.65250901117884	35.377786636694	13.365077783130038
6	27.63936490224383	38.737708523369676	18.35271334992369	15.270213224462806
7	24.019715462978713	34.52405155863664	23.986839138839557	17.469393839545088
8	26.014618341299045	32.29181925622759	21.063687454269253	20.629874948204108
9	26.093285370552334	15.253987130634867	22.3307358208399	36.321991677972896
10-11	24.76159130264409	25.714340534515156	27.92229380069125	21.6017743621495
12-13	27.08988815039129	24.40687015549284	27.596002740800625	20.90723895331525
14-15	23.736040927548423	24.911859284801054	27.676922537640834	23.675177250009686
16-17	20.731086796816566	27.658914225264443	28.958728221280168	22.651270756638823
18-19	20.454202468713067	27.508221848402144	31.280125099763595	20.757450583121198
20-21	22.256810214315998	25.688161239367314	30.186147887373316	21.868880658943375
22-23	21.87589651419599	25.60436241616929	30.07775032336777	22.44199074626695
24-25	22.433626538979105	26.055722971030832	29.97496086020109	21.535689629788976
26-27	22.324610714434627	25.11278866870074	30.89319179663761	21.66940882022702
28-29	20.9649855755456	26.993627116092462	30.755257679958838	21.286129628403103
30-31	22.038370499104296	25.49101435157768	30.420271165302353	22.050343984015676
32-33	21.867897014458357	26.354068885307264	30.10682521268368	21.6712088875507
34-35	22.307763266405548	26.228232172936032	30.577148370837616	20.8868561898208
36-37	22.30336795694842	25.31149169352409	31.020726016638594	21.36441433288889
38-39	22.3225961424928	25.654245678458416	30.76342068908516	21.25973748996363
40-41	21.625680963141278	25.770737366298025	30.651329898322295	21.9522517722384
42-43	22.046087604971397	25.845425868671875	29.31365614286774	22.79483038348899
44-45	22.39100964227784	25.715219242639147	29.568772627915525	22.32499848716749
46-47	22.930528911879016	26.123434541790875	29.458952256868532	21.487084289461574
48-49	23.298814821374542	25.982587615399943	29.094899156501143	21.623698406724376
50-51	21.528106147806454	26.601394648368508	29.371559571248113	22.498939632576924
52-53	21.705905609941087	27.58603703554059	27.569690935091035	23.138366419427285
54-55	21.68127021236439	26.899692431671625	27.640782479213634	23.778254876750353
56-57	23.302400064941413	25.610790606372007	27.729947032158915	23.356862296527662
58-59	23.114047694866212	25.906008000433932	28.477478531258775	22.50246577344108
60-61	22.445334153467858	26.859817694722143	28.845003246160832	21.849844905649167
62-63	21.921718703098534	26.483961232210994	28.383072217949167	23.211247846741298
64-65	20.896954592686182	27.890059511861043	27.976515545112118	23.236470350340657
66-67	22.730950117331805	26.119928805728453	27.55137867407957	23.59774240286017
68-69	22.013673065941962	26.0021641487451	28.18843407325819	23.79572871205475
70-71	22.70757053082752	26.121783222705062	27.717464901091347	23.453181345376066
72-73	23.062888370651354	25.256343079806943	29.18418178329471	22.496586766246992
74-75	22.545417042347665	27.460625218051803	27.677316358179294	22.31664138142124
76-77	22.47647154436445	26.164587291949527	27.697112564825368	23.66182859886066
78-79	22.477311592263057	26.624090752523994	27.98931567913755	22.909281976075402
80-81	22.464403808414755	26.64545386954591	28.076884120898555	22.813258201140783
82-83	22.504297265819076	25.646868341590245	29.22469151655063	22.624142876040047
84-85	22.572569202235517	27.03541440165127	27.519704134948764	22.872312261164446
86-87	21.870055057726393	27.29430100966531	27.695495701261425	23.140148231346874
88-89	22.173711225897577	26.37570646380481	27.371997079131695	24.078585231165917
90-91	22.600613654598913	25.682741937596592	27.694138696418452	24.02250571138604
92-93	23.024810001782186	26.65269945615697	27.632181456646236	22.690309085414608
94-95	21.515118865807406	26.629970145641007	28.475324492271113	23.379586496280474
96-97	21.303255232331708	26.604891362803716	28.78732111885547	23.304532286009106
98-99	21.919423913061525	27.224395433973775	27.303487099313635	23.552693553651068
100	22.034183486529656	25.811074634917247	27.045107280475282	25.10963459807781
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1229.0
1	780.5
2	852.0
3	1321.0
4	777.5
5	484.5
6	1119.0
7	2762.0
8	4993.0
9	5906.5
10	4062.5
11	1986.5
12	1628.5
13	1739.5
14	2039.5
15	2475.0
16	2943.5
17	3463.5
18	4167.0
19	4774.5
20	5417.0
21	6196.0
22	7136.0
23	8116.0
24	8972.5
25	10248.0
26	12191.5
27	14112.0
28	16732.5
29	21344.0
30	26840.0
31	32517.0
32	39984.5
33	46602.5
34	50794.0
35	55055.0
36	60354.5
37	68711.5
38	73157.5
39	72815.5
40	69708.5
41	64079.0
42	63383.0
43	69092.5
44	77281.5
45	90973.0
46	103672.5
47	128118.0
48	175791.5
49	178131.0
50	156882.5
51	139906.5
52	109955.5
53	97171.0
54	90370.5
55	78097.5
56	68108.5
57	56801.5
58	44885.5
59	40961.0
60	33768.5
61	23843.0
62	17311.5
63	13958.5
64	10436.0
65	6073.0
66	3972.5
67	2987.5
68	2193.5
69	1591.0
70	1259.5
71	940.5
72	682.5
73	536.5
74	345.5
75	200.0
76	167.0
77	134.5
78	93.5
79	52.5
80	23.5
81	16.0
82	6.0
83	1.5
84	0.5
85	0.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0052395488527048435
2	0.09855517595475097
3	3.3208408221368726E-4
4	0.0
5	0.037709992446932045
6	0.005497836472204378
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	7.379646271415272E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.767367351780727E-4
24-25	0.0
26-27	0.0
28-29	9.22455783926909E-5
30-31	7.379646271415272E-5
32-33	0.0010700487093552144
34-35	8.1176108985568E-4
36-37	4.612278919634546E-4
38-39	4.7967700764199264E-4
40-41	9.224557839269091E-4
42-43	5.16575238999069E-4
44-45	3.3208408221368726E-4
46-47	2.9518585085661086E-4
48-49	6.272699330702982E-4
50-51	4.243296606063782E-4
52-53	0.001125396056390829
54-55	0.0010147013623196
56-57	8.1176108985568E-4
58-59	0.0019925044932821235
60-61	0.0011991925191049816
62-63	7.564137428200655E-4
64-65	0.004021907217921323
66-67	0.003966559870885709
68-69	0.0031179005496729524
70-71	0.0011069469407122908
72-73	0.004317093068777934
74-75	0.0028965111615304943
76-77	0.0032285952437441814
78-79	6.457190487488364E-4
80-81	1.844911567853818E-5
82-83	1.2914380974976726E-4
84-85	9.22455783926909E-5
86-87	5.5347347035614544E-5
88-89	1.4759292542830543E-4
90-91	1.844911567853818E-5
92-93	0.0
94-95	0.0
96-97	4.612278919634546E-4
98-99	3.8743142924930183E-4
100	6.641681644273745E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2710157.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.28608133542815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.27679191037188	31.13979062350148
2	15.183527444724849	14.0557197253048
3	6.599784850396838	9.164345351453838
4	3.4914823627834686	6.464281465000339
5	2.084749047996138	4.824743199975284
6	1.3592799038932595	3.7749444113529824
7	0.926454727585263	3.0017371212222392
8	0.6297496681784626	2.331891548981377
9	0.4906812599866527	2.044054143856132
>10	1.8953856799498643	13.925762336322519
>50	0.04069595851112675	1.27203498593801
>100	0.01713385281702686	1.450475772985596
>500	0.0021416664025260728	0.7393810042614143
>1k	0.0013385415015787954	1.0194833605766358
>5k	4.015624504736386E-4	1.3035508907038842
>10k+	4.015624504736386E-4	3.4878040585634587
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	26105	0.9632283295764784	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15527	0.5729188382813247	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14663	0.5410387663888107	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8286	0.3057387450247347	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	7447	0.27478112891614764	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	5307	0.19581891381200428	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	3.6898231357076365E-5	0.0	0.0	0.0
12-13	0.0	3.6898231357076365E-5	0.0	0.0	0.0
14-15	0.0	3.6898231357076365E-5	0.0	3.6898231357076365E-5	0.0
16-17	0.0	3.6898231357076365E-5	0.0	3.6898231357076365E-5	0.0
18-19	0.0	3.6898231357076365E-5	0.0	7.379646271415273E-5	0.0
20-21	0.0	3.6898231357076365E-5	0.0	2.9518585085661086E-4	0.0
22-23	0.0	3.6898231357076365E-5	0.0	7.748628584986036E-4	0.0
24-25	0.0	3.6898231357076365E-5	0.0	0.0018633606835323563	0.0
26-27	0.0	3.6898231357076365E-5	0.0	0.004095703680635476	0.0
28-29	0.0	3.6898231357076365E-5	0.0	0.011493799067729286	0.0
30-31	0.0	3.6898231357076365E-5	0.0	0.025791863718596375	0.0
32-33	0.0	3.6898231357076365E-5	0.0	0.054498687714401785	0.0
34-35	0.0	3.6898231357076365E-5	0.0	0.09912709854078564	0.0
36-37	0.0	3.6898231357076365E-5	0.0	0.16344071579616976	0.0
38-39	0.0	3.6898231357076365E-5	0.0	0.2551328207185045	0.0
40-41	0.0	3.6898231357076365E-5	0.0	0.35944412076495935	0.0
42-43	0.0	3.6898231357076365E-5	0.0	0.4533132213373616	0.0
44-45	0.0	7.379646271415273E-5	0.0	0.5607424219334894	0.0
46-47	0.0	7.379646271415273E-5	0.0	0.6890929197090796	0.0
48-49	0.0	7.379646271415273E-5	0.0	0.827221448794295	0.0
50-51	0.0	7.379646271415273E-5	0.0	0.9663462301261514	0.0
52-53	0.0	7.379646271415273E-5	0.0	1.0999362767544465	0.0
54-55	0.0	7.379646271415273E-5	0.0	1.2379172129142333	0.0
56-57	0.0	7.379646271415273E-5	0.0	1.4088298205602112	0.0
58-59	0.0	7.379646271415273E-5	0.0	1.5915498622404534	0.0
60-61	0.0	7.379646271415273E-5	0.0	1.773089160517269	0.0
62-63	0.0	7.379646271415273E-5	0.0	1.9508832883113414	0.0
64-65	0.0	7.379646271415273E-5	0.0	2.1401158678261076	0.0
66-67	0.0	7.379646271415273E-5	0.0	2.3475946227469477	0.0
68-69	0.0	7.379646271415273E-5	0.0	2.5819537392114182	0.0
70-71	0.0	7.379646271415273E-5	0.0	2.8398539272817036	0.0
72-73	0.0	7.379646271415273E-5	0.0	3.0764638358589558	0.0
74-75	0.0	7.379646271415273E-5	0.0	3.3119667974954954	0.0
76-77	0.0	7.379646271415273E-5	0.0	3.5638525738545774	0.0
78-79	0.0	7.379646271415273E-5	0.0	3.8498138668719193	0.0
80-81	0.0	7.379646271415273E-5	0.0	4.151918136107982	0.0
82-83	0.0	7.379646271415273E-5	0.0	4.460110613517962	0.0
84-85	0.0	7.379646271415273E-5	0.0	4.762749907108702	0.0
86-87	0.0	7.379646271415273E-5	0.0	5.070185970775863	0.0
88	0.0	7.379646271415273E-5	0.0	5.318621762503058	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCGC	65	0.0061582387	28.920563	9
GTACTAG	1660	0.0	26.087801	1
GAGTACT	14115	0.0	23.922468	12-13
AGGGCTA	2625	0.0	22.746723	5
TAGGCAT	2500	0.0	22.567614	5
GGGCACC	1865	0.0	22.427006	94
GGGCTAT	2720	0.0	22.288914	6
GCTATTG	2640	0.0	22.251852	8
AGTACTT	14365	0.0	21.968504	12-13
GTACTTT	15465	0.0	21.849377	14-15
CTGTGCG	2055	0.0	21.725605	9
ATTTAGG	2890	0.0	21.49977	1
GTGATCG	1190	0.0	20.930954	8
CTATTGA	2720	0.0	20.90627	9
CATGGGG	7785	0.0	20.714762	4
GGCTATT	2875	0.0	20.433006	7
TGATCGC	1240	0.0	20.086964	9
CTAGGCA	2905	0.0	20.068733	4
TACCTGG	2370	0.0	19.861378	2
ACTTTTT	17035	0.0	19.849463	16-17
>>END_MODULE