FastQCFastQC Report
Mon 16 Jan 2017
ERR1146540_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146540_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4899358
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA680461.3888758486315962No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA539181.1005115364094642No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT105710.21576296322905983No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA84050.1715530892006667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18900.023.8821161
CATGGGG91650.020.6633594
GCATCGT20200.020.4711368
GTACCCG6500.018.8071651
TATTCGC4250.018.7962259
CTCGTCG14200.018.5314879
ATGGGGG63500.018.204965
GCTATTG28050.018.0926238
CTGTGCG25750.018.0662739
TGATCGC16550.017.6037149
TACCTGG39600.017.5628452
GGGCTAT33300.017.4983446
CTATTGA30100.017.484869
GTGATCG16300.017.297148
CCGTATC5200.017.17471194
GTATATA26350.016.951431
ACGTTAA5550.016.9335358
CTAGTAC28950.016.7194293
GTCCTAC17750.016.6880471
TCTAACG11550.016.6813132
Produced by FastQC (version 0.11.5)