##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146540_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4899358 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.656614193124895 33.0 31.0 34.0 30.0 34.0 2 31.8263868449703 33.0 31.0 34.0 30.0 34.0 3 31.889858834565672 34.0 31.0 34.0 30.0 34.0 4 35.38085255251811 37.0 35.0 37.0 33.0 37.0 5 35.432868551348975 37.0 35.0 37.0 33.0 37.0 6 35.42661814058087 37.0 35.0 37.0 33.0 37.0 7 35.42995225088675 37.0 35.0 37.0 33.0 37.0 8 35.402521105826516 37.0 35.0 37.0 33.0 37.0 9 37.04910378053614 39.0 38.0 39.0 34.0 39.0 10-11 37.039421593604715 39.0 38.0 39.0 34.0 39.0 12-13 37.01643807209026 39.0 38.0 39.0 34.0 39.0 14-15 38.4131962800024 40.5 39.0 41.0 34.0 41.0 16-17 38.409213309172344 40.0 39.0 41.0 34.0 41.0 18-19 38.469977597064755 40.5 39.0 41.0 34.0 41.0 20-21 38.552408397181836 41.0 39.0 41.0 34.0 41.0 22-23 38.55849439865386 41.0 39.0 41.0 34.0 41.0 24-25 38.592001441821566 41.0 39.0 41.0 34.0 41.0 26-27 38.565824644779994 41.0 39.0 41.0 34.0 41.0 28-29 38.50513067222277 40.0 38.5 41.0 34.0 41.0 30-31 38.50877574163799 40.0 38.5 41.0 34.0 41.0 32-33 38.5213304477852 40.0 38.0 41.0 34.0 41.0 34-35 38.46194470785764 40.0 38.0 41.0 33.5 41.0 36-37 38.33690434542648 40.0 38.0 41.0 33.0 41.0 38-39 38.2590828226882 40.0 38.0 41.0 33.0 41.0 40-41 38.17983060229524 40.0 38.0 41.0 33.0 41.0 42-43 38.08566540759014 40.0 38.0 41.0 33.0 41.0 44-45 37.97882630744681 40.0 38.0 41.0 33.0 41.0 46-47 37.85724007104604 40.0 38.0 41.0 32.5 41.0 48-49 37.71906115046094 40.0 37.0 41.0 32.0 41.0 50-51 37.57210903959253 40.0 37.0 41.0 32.0 41.0 52-53 37.38881951472009 40.0 36.0 41.0 31.0 41.0 54-55 37.23090127318722 40.0 36.0 41.0 31.0 41.0 56-57 37.12174911488403 39.0 36.0 41.0 31.0 41.0 58-59 36.91037529815131 39.0 35.0 41.0 31.0 41.0 60-61 36.68570096326906 39.0 35.0 41.0 30.5 41.0 62-63 36.38259829144961 38.5 35.0 40.0 30.0 41.0 64-65 36.03626128158016 37.5 35.0 40.0 30.0 41.0 66-67 35.77323590152016 37.0 35.0 40.0 29.5 41.0 68-69 35.532288924385604 37.0 35.0 39.5 29.5 41.0 70-71 35.22629240402518 36.0 34.0 39.0 29.0 41.0 72-73 34.833666676327795 35.5 34.0 39.0 29.0 41.0 74-75 34.46614536026965 35.0 34.0 37.5 29.0 40.0 76-77 34.0950613325256 35.0 34.0 37.0 28.0 39.0 78-79 33.836721056105716 35.0 34.0 37.0 28.0 39.0 80-81 33.61418108250102 35.0 34.0 36.0 28.0 39.0 82-83 33.38730666344448 35.0 33.5 36.0 28.0 37.0 84-85 33.179148880322685 35.0 33.0 35.5 27.0 37.0 86-87 32.92157288771304 35.0 33.0 35.0 27.0 37.0 88-89 32.707928059145715 35.0 33.0 35.0 26.5 36.0 90-91 32.51786713687793 35.0 33.0 35.0 26.0 36.0 92-93 32.484947925830284 35.0 33.0 35.0 26.0 36.0 94-95 32.466871067597026 35.0 33.0 35.0 26.0 36.0 96-97 32.367257301875064 35.0 33.0 35.0 26.0 35.0 98-99 32.23857615630456 35.0 33.0 35.0 25.0 35.0 100 31.156688080356652 34.0 31.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22499649769475738 1101 2 0.29263588377385474 1101 3 0.3065943140183158 1101 4 0.2961119513187711 1101 5 0.27970302234436417 1101 6 0.2869262465122091 1101 7 0.30618889921910863 1101 8 0.29221305751103444 1101 9 0.41017861395857125 1101 10-11 0.40261716534170944 1101 12-13 0.3676579410695595 1101 14-15 0.45367297455053546 1101 16-17 0.4178711267732922 1101 18-19 0.4095974906351145 1101 20-21 0.43211772005071225 1101 22-23 0.4048424162356099 1101 24-25 0.43041395235553637 1101 26-27 0.38756575754234746 1101 28-29 0.46239412828535364 1101 30-31 0.43877217041936234 1101 32-33 0.43130235379744875 1101 34-35 0.4421188614355529 1101 36-37 0.41064111545485815 1101 38-39 0.41380486641701 1101 40-41 0.3781722780783525 1101 42-43 0.36559717580929885 1101 44-45 0.3596384327738136 1101 46-47 0.31144366370364907 1101 48-49 0.32475969865931376 1101 50-51 0.33840997337090073 1101 52-53 0.32741200054403663 1101 54-55 0.3998405864740704 1101 56-57 0.2814163675557353 1101 58-59 0.33919069435606986 1101 60-61 0.39396355622867674 1101 62-63 0.4491497435299223 1101 64-65 0.3824711151256821 1101 66-67 0.3571230014251512 1101 68-69 0.34995569488727796 1101 70-71 0.34049433904846893 1101 72-73 0.3567071144145899 1101 74-75 0.37567730301503843 1101 76-77 0.3833238274335642 1101 78-79 0.36908189901603095 1101 80-81 0.3315998376886853 1101 82-83 0.23109855133846935 1101 84-85 0.30539154577444094 1101 86-87 0.32894483623305604 1101 88-89 0.26670123929964973 1101 90-91 0.3101824280686003 1101 92-93 0.29339645025940086 1101 94-95 0.2594033008056442 1101 96-97 0.3078625170818867 1101 98-99 0.27342154699074683 1101 100 0.2141462142498085 1102 1 0.21021323072783815 1102 2 0.23862369959513785 1102 3 0.2465585949547382 1102 4 0.19570378694949397 1102 5 0.21707076371070855 1102 6 0.17888684610479544 1102 7 0.1976359644532124 1102 8 0.2674763888588174 1102 9 0.3746425877002295 1102 10-11 0.3707391286335451 1102 12-13 0.34157496769054063 1102 14-15 0.4454162699961657 1102 16-17 0.4447845038829996 1102 18-19 0.3854097778847958 1102 20-21 0.3838317066398389 1102 22-23 0.39198728275412975 1102 24-25 0.34083211485855003 1102 26-27 0.3627782711986356 1102 28-29 0.3837097208175564 1102 30-31 0.39076809897041187 1102 32-33 0.3380716031956936 1102 34-35 0.30078890575357775 1102 36-37 0.3591716623489205 1102 38-39 0.36474912362255907 1102 40-41 0.32628988571667605 1102 42-43 0.2949807140397027 1102 44-45 0.29442461460607205 1102 46-47 0.2824108195586561 1102 48-49 0.3175015331353279 1102 50-51 0.2838781171800804 1102 52-53 0.25255702081597775 1102 54-55 0.3047338984497614 1102 56-57 0.29998183353783503 1102 58-59 0.29430681356137 1102 60-61 0.2430175617550674 1102 62-63 0.3115382176821768 1102 64-65 0.24361952317437385 1102 66-67 0.2349729532705851 1102 68-69 0.2087165296664324 1102 70-71 0.15762396338037377 1102 72-73 0.18933912546152953 1102 74-75 0.1877020639742284 1102 76-77 0.2054217523448827 1102 78-79 0.20408395460985673 1102 80-81 0.20800543839124686 1102 82-83 0.17005620506536445 1102 84-85 0.2484086592172119 1102 86-87 0.24830647032195685 1102 88-89 0.2009203643531947 1102 90-91 0.25462657198763594 1102 92-93 0.23817168938342803 1102 94-95 0.21891723088674553 1102 96-97 0.20212343643582642 1102 98-99 0.16206172798287355 1102 100 0.06894722215655946 1103 1 0.1830338268356435 1103 2 0.14887658046483665 1103 3 0.17668766119876622 1103 4 0.14811784500008685 1103 5 0.1478472425780879 1103 6 0.15690543025025505 1103 7 0.14486312515486333 1103 8 0.14174710011228342 1103 9 0.23402954405771226 1103 10-11 0.25933217297898636 1103 12-13 0.29240896975695563 1103 14-15 0.3444579078852783 1103 16-17 0.28708998915533357 1103 18-19 0.25679837870276145 1103 20-21 0.2716033310266681 1103 22-23 0.2920376289102222 1103 24-25 0.2615589737820585 1103 26-27 0.23253770555122344 1103 28-29 0.24331631332550074 1103 30-31 0.2767218759171186 1103 32-33 0.31247439492968 1103 34-35 0.2988912965110515 1103 36-37 0.3195899185543638 1103 38-39 0.27854908780824417 1103 40-41 0.29656923281846304 1103 42-43 0.3014122246816484 1103 44-45 0.2634846063189471 1103 46-47 0.21709399352537417 1103 48-49 0.2649849571135192 1103 50-51 0.2506787482250772 1103 52-53 0.3283969227236483 1103 54-55 0.3028661302498108 1103 56-57 0.22949739225482801 1103 58-59 0.3209887316975042 1103 60-61 0.22972753629509413 1103 62-63 0.25039989724594847 1103 64-65 0.2857775731861878 1103 66-67 0.2557040712211176 1103 68-69 0.22061243276601772 1103 70-71 0.22713370277755018 1103 72-73 0.26973742703650316 1103 74-75 0.19174468331333117 1103 76-77 0.2071911982976289 1103 78-79 0.20743803968525754 1103 80-81 0.2626296124261316 1103 82-83 0.20821336508739563 1103 84-85 0.18779965329438397 1103 86-87 0.25069200656486146 1103 88-89 0.2505646925496663 1103 90-91 0.28785485776115394 1103 92-93 0.2552003151093558 1103 94-95 0.22776363771123442 1103 96-97 0.17040464910287056 1103 98-99 0.12346000352764008 1103 100 0.14800068910079034 1104 1 0.1968745046200162 1104 2 0.2036286654802737 1104 3 0.201656195670342 1104 4 0.23464002789959437 1104 5 0.17590018610783886 1104 6 0.16052100636964184 1104 7 0.16925068311235236 1104 8 0.21410002808306672 1104 9 0.2259161979500277 1104 10-11 0.28387974675462857 1104 12-13 0.2630357688431104 1104 14-15 0.30244142718846234 1104 16-17 0.3175156213169572 1104 18-19 0.24650780475456457 1104 20-21 0.23264447795470744 1104 22-23 0.3033728558053781 1104 24-25 0.23242105761551812 1104 26-27 0.2827538018559821 1104 28-29 0.279360085872689 1104 30-31 0.27074508386447604 1104 32-33 0.3049875502317505 1104 34-35 0.3504951408282295 1104 36-37 0.31882530804316644 1104 38-39 0.3184376897931145 1104 40-41 0.3352528001393722 1104 42-43 0.31943929255472625 1104 44-45 0.21289714631375034 1104 46-47 0.20246403656711465 1104 48-49 0.24861766532405483 1104 50-51 0.25101761774926246 1104 52-53 0.25031180016276267 1104 54-55 0.2788122130042723 1104 56-57 0.3052517275008242 1104 58-59 0.2712560349525859 1104 60-61 0.29555253415812643 1104 62-63 0.22630502293845467 1104 64-65 0.2677391034336125 1104 66-67 0.2599654351592733 1104 68-69 0.2666945451362821 1104 70-71 0.23242124767362782 1104 72-73 0.26893370076064116 1104 74-75 0.2581534196364146 1104 76-77 0.2368193126602236 1104 78-79 0.24997074460742397 1104 80-81 0.24185391258384925 1104 82-83 0.29065215251569043 1104 84-85 0.2823610228720881 1104 86-87 0.24737440192364346 1104 88-89 0.2274874182825073 1104 90-91 0.24883453368668995 1104 92-93 0.24858816925603833 1104 94-95 0.22879034981262691 1104 96-97 0.2594634433076095 1104 98-99 0.22846707635191876 1104 100 0.2001465594120333 1105 1 0.15948642525006917 1105 2 0.14080411602844833 1105 3 0.1488110113115475 1105 4 0.13028276270933503 1105 5 0.12535270491115114 1105 6 0.06307360978136245 1105 7 0.08551239608450345 1105 8 0.07658204480905795 1105 9 0.09971927043125817 1105 10-11 0.1681096876773296 1105 12-13 0.18371483072943562 1105 14-15 0.21581137332636757 1105 16-17 0.198215867424679 1105 18-19 0.18968857958856944 1105 20-21 0.21099935902419986 1105 22-23 0.20116079019477695 1105 24-25 0.2340668923590954 1105 26-27 0.23643087419273456 1105 28-29 0.29428548770579965 1105 30-31 0.3290196098906861 1105 32-33 0.29704631866005826 1105 34-35 0.23468470255178886 1105 36-37 0.2661233627752395 1105 38-39 0.2767099281017096 1105 40-41 0.27637854030997744 1105 42-43 0.2790200747478835 1105 44-45 0.2721260132983332 1105 46-47 0.21005935736958037 1105 48-49 0.17468441144101376 1105 50-51 0.23384154534394241 1105 52-53 0.20931398863670125 1105 54-55 0.30244494775676856 1105 56-57 0.20545568397186997 1105 58-59 0.2787100176329673 1105 60-61 0.27129208640087654 1105 62-63 0.3034084372575734 1105 64-65 0.30661216718218753 1105 66-67 0.2755784960646963 1105 68-69 0.2278698686910019 1105 70-71 0.24287114624274864 1105 72-73 0.2932967025066091 1105 74-75 0.2945870050964814 1105 76-77 0.26437656470539395 1105 78-79 0.3126452377009912 1105 80-81 0.3036179618029493 1105 82-83 0.224310209920489 1105 84-85 0.21009900460872188 1105 86-87 0.2276400079050731 1105 88-89 0.20869888733851383 1105 90-91 0.24827548498566188 1105 92-93 0.20327040615354264 1105 94-95 0.2045874882995946 1105 96-97 0.12797589021759137 1105 98-99 0.18576896841892676 1105 100 0.22264502760264193 1106 1 0.1701771123495277 1106 2 0.18099963817083875 1106 3 0.21261077415386964 1106 4 0.14109234688111627 1106 5 0.18380306256752732 1106 6 0.19906947964167898 1106 7 0.1635201811105631 1106 8 0.133618994937315 1106 9 0.2563505342855734 1106 10-11 0.18751797553394312 1106 12-13 0.20143116446545406 1106 14-15 0.2143788091120058 1106 16-17 0.2467851853809151 1106 18-19 0.21444497458244172 1106 20-21 0.1962511078202951 1106 22-23 0.24141073616873143 1106 24-25 0.22412370334407683 1106 26-27 0.22330166655222428 1106 28-29 0.2668564209370672 1106 30-31 0.22230602253257103 1106 32-33 0.1983325111361438 1106 34-35 0.23104720375091858 1106 36-37 0.25351000386951483 1106 38-39 0.20178455857342215 1106 40-41 0.1832217838891097 1106 42-43 0.19977592975349268 1106 44-45 0.1507524839194545 1106 46-47 0.18731125305453844 1106 48-49 0.15898634451605886 1106 50-51 0.21319370473795374 1106 52-53 0.18217910785346447 1106 54-55 0.18513122266313076 1106 56-57 0.10640769172029962 1106 58-59 0.21272980517087348 1106 60-61 0.1980309932454105 1106 62-63 0.16844689236859978 1106 64-65 0.1813057073089155 1106 66-67 0.15976982701723585 1106 68-69 0.10000079900961367 1106 70-71 0.11085690463878706 1106 72-73 0.18172496904926305 1106 74-75 0.10689350948025833 1106 76-77 0.17832079408550783 1106 78-79 0.12380767965885298 1106 80-81 0.13707948791208224 1106 82-83 0.08934039520521253 1106 84-85 0.08176127654314058 1106 86-87 0.05877459331941992 1106 88-89 0.0886807510860308 1106 90-91 0.18454293803534227 1106 92-93 0.09033301032980035 1106 94-95 0.09821789431121886 1106 96-97 0.09817201197472514 1106 98-99 0.0657078290696802 1106 100 0.004811396325262507 1107 1 0.08076178378014376 1107 2 0.11698917784598706 1107 3 0.15338683857071445 1107 4 0.09480174800553698 1107 5 0.06818866136961077 1107 6 0.016340590187489568 1107 7 0.0848865883650447 1107 8 0.07626220245172277 1107 9 0.13957758183371283 1107 10-11 0.1550636335113893 1107 12-13 0.09638443782693429 1107 14-15 0.15579381668920433 1107 16-17 0.11278662286591867 1107 18-19 0.08425240100865494 1107 20-21 0.10416585655566735 1107 22-23 0.13199101948302427 1107 24-25 0.17243859707633646 1107 26-27 0.18061077287276817 1107 28-29 0.1520006270151697 1107 30-31 0.19725172098900856 1107 32-33 0.19350569286130082 1107 34-35 0.18164716259387603 1107 36-37 0.21336403418903416 1107 38-39 0.21129276548167297 1107 40-41 0.23905759271711702 1107 42-43 0.2527246236716749 1107 44-45 0.2135627433768974 1107 46-47 0.15759301244030155 1107 48-49 0.17448151634170017 1107 50-51 0.18147457760615993 1107 52-53 0.1713795435620682 1107 54-55 0.1982092411737426 1107 56-57 0.13559967346475332 1107 58-59 0.2387042072007759 1107 60-61 0.20075576739472467 1107 62-63 0.15622309476661655 1107 64-65 0.22176038468214898 1107 66-67 0.24232104602075566 1107 68-69 0.20907448588066302 1107 70-71 0.1861783609820833 1107 72-73 0.18707983103489312 1107 74-75 0.2071430222735131 1107 76-77 0.14055997023640998 1107 78-79 0.12876956752046453 1107 80-81 0.1442335094988536 1107 82-83 0.21072049089382716 1107 84-85 0.14987922433974887 1107 86-87 0.1156893634529439 1107 88-89 0.13335743491848007 1107 90-91 0.19108693288000467 1107 92-93 0.1227612278641459 1107 94-95 0.126367678486055 1107 96-97 0.11465431054998021 1107 98-99 0.11144523692178154 1107 100 0.08321256089512019 1108 1 0.12469550228964366 1108 2 0.08948102247017076 1108 3 0.12499235858958357 1108 4 0.07509462188239979 1108 5 0.0762360893114149 1108 6 0.08938331682360001 1108 7 0.05770433382845397 1108 8 0.0801543479385245 1108 9 0.07705855723664001 1108 10-11 0.1378661551826852 1108 12-13 0.09794297733035506 1108 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-0.25819574669252177 2214 90-91 -0.2766776847537855 2214 92-93 -0.28105987043435476 2214 94-95 -0.22993251211615728 2214 96-97 -0.31385722415962647 2214 98-99 -0.28367544145049095 2214 100 -0.2552126448480756 2215 1 -0.32260849149193405 2215 2 -0.3697422025376724 2215 3 -0.3555471048019072 2215 4 -0.28581844754819485 2215 5 -0.345813685773237 2215 6 -0.32138948915879695 2215 7 -0.315402043509053 2215 8 -0.2708404368723336 2215 9 -0.4195468298892706 2215 10-11 -0.41987754009072376 2215 12-13 -0.43279298337552774 2215 14-15 -0.49303017442148445 2215 16-17 -0.4683956382295307 2215 18-19 -0.537419563756167 2215 20-21 -0.5143844957920578 2215 22-23 -0.4604063756063397 2215 24-25 -0.5099182691148485 2215 26-27 -0.4368650406576293 2215 28-29 -0.4432971470925082 2215 30-31 -0.5126983276418215 2215 32-33 -0.5569978222005787 2215 34-35 -0.5269265055363093 2215 36-37 -0.5525234200534399 2215 38-39 -0.578612621214603 2215 40-41 -0.5448355360089892 2215 42-43 -0.5359337921839895 2215 44-45 -0.4762867537172184 2215 46-47 -0.44675879082972614 2215 48-49 -0.420197687484972 2215 50-51 -0.4903035087397569 2215 52-53 -0.493168283506229 2215 54-55 -0.4918861096987541 2215 56-57 -0.4368639422538152 2215 58-59 -0.43981906630403245 2215 60-61 -0.43474286402941686 2215 62-63 -0.46172044639851606 2215 64-65 -0.5047044426331411 2215 66-67 -0.4922103440770087 2215 68-69 -0.43668968951885745 2215 70-71 -0.47817812567097917 2215 72-73 -0.45366407321307634 2215 74-75 -0.527072138187691 2215 76-77 -0.5267602932847595 2215 78-79 -0.5096490364545616 2215 80-81 -0.4635513919707037 2215 82-83 -0.39519053605884835 2215 84-85 -0.36553317796570894 2215 86-87 -0.41180141280409543 2215 88-89 -0.4561235548195839 2215 90-91 -0.44290143262779935 2215 92-93 -0.461079237378506 2215 94-95 -0.46143328685027996 2215 96-97 -0.44303022600919917 2215 98-99 -0.45235311481157936 2215 100 -0.34660539381383515 2216 1 -0.1516757018967283 2216 2 -0.22035979395497307 2216 3 -0.15087774070524773 2216 4 -0.19686736260035076 2216 5 -0.1429660935372823 2216 6 -0.11761371815665456 2216 7 -0.14022191963845643 2216 8 -0.14328351348896717 2216 9 -0.15929080861959477 2216 10-11 -0.22170501535806864 2216 12-13 -0.21586780904191727 2216 14-15 -0.29881674033999417 2216 16-17 -0.389491601821085 2216 18-19 -0.42018068980862466 2216 20-21 -0.39680915203096845 2216 22-23 -0.31971984712855317 2216 24-25 -0.4219167089514144 2216 26-27 -0.38486397449250376 2216 28-29 -0.39950766698040496 2216 30-31 -0.4255720767117097 2216 32-33 -0.45559980944472045 2216 34-35 -0.4564690172729371 2216 36-37 -0.40993905573451883 2216 38-39 -0.47657036959428467 2216 40-41 -0.5083907898375344 2216 42-43 -0.542108854520599 2216 44-45 -0.4683155636930678 2216 46-47 -0.3780651161459829 2216 48-49 -0.39757961583236323 2216 50-51 -0.5250680437858435 2216 52-53 -0.42247892314562563 2216 54-55 -0.42719584899717944 2216 56-57 -0.5236761829002461 2216 58-59 -0.5646106336269057 2216 60-61 -0.564040072491359 2216 62-63 -0.5636026140172916 2216 64-65 -0.5483601732940926 2216 66-67 -0.5670112682382182 2216 68-69 -0.5526812578305211 2216 70-71 -0.5750742331193379 2216 72-73 -0.5707740526202372 2216 74-75 -0.593293761345592 2216 76-77 -0.5379436359166618 2216 78-79 -0.5068285894054725 2216 80-81 -0.49523159441069 2216 82-83 -0.5108010050102649 2216 84-85 -0.462057389227553 2216 86-87 -0.5228632479318591 2216 88-89 -0.5816427351912807 2216 90-91 -0.6131957513488651 2216 92-93 -0.574998187493815 2216 94-95 -0.4979516361414227 2216 96-97 -0.5517449790229634 2216 98-99 -0.573751789884156 2216 100 -0.633424959139866 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 156.0 1 61.0 2 51.0 3 68.0 4 38.0 5 72.0 6 167.0 7 810.0 8 2570.0 9 3641.0 10 3953.0 11 4440.0 12 5502.0 13 6787.0 14 7947.0 15 9314.0 16 10116.0 17 10894.0 18 11986.0 19 12998.0 20 14918.0 21 17044.0 22 19630.0 23 23737.0 24 29759.0 25 36764.0 26 41144.0 27 40677.0 28 45046.0 29 56199.0 30 65212.0 31 76250.0 32 97562.0 33 133003.0 34 201511.0 35 321313.0 36 584441.0 37 1169394.0 38 1375060.0 39 458008.0 40 1115.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.60388750883567 18.903595311527017 12.72399087565041 23.76852630398691 2 14.11078160161213 21.402553734906704 40.19972793692578 24.286936726555382 3 19.46647434632074 27.034107126052636 30.237155400813798 23.262263126812826 4 12.10837612385726 16.935392177575824 40.00526652602184 30.950965172545068 5 13.156691425302963 38.977929525867964 35.111340375384636 12.754038673444434 6 28.617274404149985 38.148833286110076 19.153120940262713 14.080771369477226 7 25.99642880178707 33.834531216958865 22.447438314368316 17.72160166688575 8 24.132076307435053 34.25214586339427 21.657120310621778 19.958657518548904 9 26.000957341163527 17.362004578499423 21.090674905107896 35.54636317522915 10-11 23.7249864335015 25.747055556967403 29.016426052045347 21.51153195748575 12-13 26.972489835809387 23.522604939476167 27.46384920215339 22.041056022561055 14-15 24.085247171930806 25.07161393612402 26.45652427300602 24.38661461893915 16-17 21.80298499419407 27.918833535199596 28.124054310070047 22.15412716053629 18-19 21.34804201990033 29.7583397471037 28.058460109274115 20.83515812372186 20-21 22.63877341773676 28.37808488284676 26.148145003144514 22.83499669627196 22-23 22.35994576287239 27.773228307241123 25.19032084041013 24.67650508947636 24-25 22.87654120651172 27.88009793505628 26.326823040252776 22.91653781817922 26-27 24.07206803381769 26.65087190628959 26.651586324244096 22.62547373564862 28-29 22.446044619281132 26.974771424978893 28.10155156675338 22.477632388986596 30-31 22.45471264381894 28.84569357907364 26.933972985631605 21.765620791475822 32-33 22.18940941540663 28.64100783196813 25.979730884858792 23.189851867766453 34-35 22.713417683689837 27.463983845754058 26.494986296571348 23.327612173984758 36-37 23.886660008094402 27.118837141767877 26.975904100227677 22.018598749910048 38-39 24.675072275627596 25.850080091898732 27.554950963424957 21.91989666904872 40-41 22.685983578637796 26.64942170833489 28.673587990780327 21.991006722246986 42-43 22.29028619773162 28.04491555467813 27.2014065662693 22.463391681320953 44-45 22.39057459033229 28.675259708362237 26.201992080839627 22.732173620465847 46-47 22.19293516570491 27.488135934772572 25.670185571161063 24.64874332836145 48-49 23.178110652779303 27.16288188177857 26.471086354592018 23.187921110850112 50-51 24.11440597853806 27.513390744307735 26.29648375817155 22.07571951898266 52-53 22.2136912777439 27.62232368839791 27.497422086053785 22.6665629478044 54-55 21.668250447875902 27.3027681936441 27.797054942847073 23.23192641563292 56-57 23.038232324062488 28.276150257681937 25.899538603387278 22.786078814868297 58-59 22.18520603080332 27.44133732811454 26.904100034374284 23.469356606707855 60-61 22.57335937510369 26.61849122917707 27.267422172033516 23.54072722368572 62-63 24.579769243278886 26.277128370332083 26.279282418242396 22.863819968146633 64-65 23.388392324818238 26.77944306419234 27.521828809536643 22.310335801452776 66-67 22.645064546638068 26.702914767075086 27.843680424310634 22.808340261976205 68-69 22.13580332496937 28.412115126020478 27.063021316317226 22.389060232692927 70-71 22.24330924586977 27.96133890632434 25.904290617732155 23.89106123007373 72-73 22.561421136448118 27.344486888122688 25.648483242256436 24.445608733172758 74-75 23.182175277324454 27.551923519655823 26.29651957874146 22.969381624278263 76-77 23.8855622428786 26.34637279652524 26.877593674476653 22.890471286119507 78-79 22.72719058910008 26.578145538168613 27.851281645529234 22.843382227202074 80-81 22.25534605488619 28.444077982479865 27.060963132694386 22.239612829939563 82-83 22.487091786094147 27.766917279415892 26.50769388150559 23.23829705298437 84-85 23.04717417035363 26.912990471570193 26.057295944858556 23.982539413217623 86-87 23.461383208986536 27.041921691429515 26.920773656841224 22.575921442742725 88-89 24.59055320031655 25.41185603087736 27.335086083055593 22.662504685750502 90-91 23.047734494770175 26.307297886070202 28.180769179332493 22.464198439827133 92-93 22.502688272760377 27.839041142390098 27.208117588363343 22.45015299648618 94-95 22.041484893371972 28.26098253393921 26.641288935030634 23.056243637658184 96-97 22.39513456134466 27.143294371738413 25.692295347496767 24.769275719420165 98-99 23.001330992985284 27.131849974080446 26.080255263179925 23.786563769754345 100 22.745727512195877 28.58337522146948 26.37558121064948 22.295316055685156 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2566.0 1 1664.5 2 766.5 3 790.0 4 772.5 5 603.5 6 452.0 7 440.0 8 489.0 9 546.0 10 470.0 11 384.5 12 390.0 13 423.5 14 477.0 15 564.5 16 696.5 17 848.5 18 1098.5 19 1538.5 20 2297.5 21 3371.5 22 5199.5 23 7503.5 24 10058.0 25 13624.5 26 18507.5 27 24451.0 28 30348.5 29 38287.5 30 49205.5 31 58707.5 32 70018.5 33 82019.5 34 91699.5 35 102463.5 36 113004.5 37 125095.0 38 131389.5 39 130774.0 40 130112.0 41 129255.0 42 131907.5 43 138788.5 44 150864.5 45 167919.5 46 185038.5 47 213920.0 48 382866.0 49 391453.0 50 243160.5 51 212333.5 52 176494.5 53 160068.5 54 150702.5 55 134757.0 56 121351.5 57 105756.0 58 89830.0 59 81092.0 60 68144.5 61 52723.0 62 41219.5 63 32223.0 64 23089.0 65 15389.0 66 11356.5 67 8515.0 68 6274.5 69 4710.5 70 3531.5 71 2662.0 72 2094.0 73 1920.0 74 1477.0 75 1001.5 76 728.0 77 516.5 78 389.5 79 254.0 80 177.0 81 119.5 82 82.5 83 58.0 84 44.0 85 40.0 86 28.0 87 22.0 88 24.5 89 25.0 90 17.5 91 13.5 92 13.0 93 13.5 94 14.0 95 9.5 96 6.5 97 6.0 98 9.0 99 10.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0614162100422137 2 0.010082953725773867 3 0.011981161613419554 4 0.0100625428882723 5 0.012164859150933653 6 0.013103757676005714 7 0.003306555675253778 8 0.005735445337940195 9 0.0076132423880843165 10-11 0.00697030100678497 12-13 0.006041607900463693 14-15 0.010634046338316164 16-17 0.009950283282013684 18-19 0.00803166455686643 20-21 0.007082560613043587 22-23 0.0026738197127052156 24-25 0.003939291637802341 26-27 0.005408871937915131 28-29 0.006327359625485625 30-31 0.009113438944449458 32-33 0.010725895107073212 34-35 0.011919929100914856 36-37 0.018584067545176327 38-39 0.018828997595195126 40-41 0.01880858675769356 42-43 0.024258280370611823 44-45 0.02784038235213675 46-47 0.030197834083567684 48-49 0.03109591093363661 50-51 0.03208583655246259 52-53 0.03066728334610371 54-55 0.03665786415281349 56-57 0.03968887352179612 58-59 0.036770123759072106 60-61 0.03758655725913477 62-63 0.03270836709626036 64-65 0.03473924542766624 66-67 0.03702525922784169 68-69 0.03999503608431962 70-71 0.03935209470302028 72-73 0.035851636071501616 74-75 0.03858668829671153 76-77 0.03808662277792315 78-79 0.037668200609141035 80-81 0.04242392574700604 82-83 0.038678537065468574 84-85 0.03467801291516154 86-87 0.03329007596505501 88-89 0.031228581377396796 90-91 0.0333002813838058 92-93 0.03462698582140762 94-95 0.0385458666217084 96-97 0.03324925429005188 98-99 0.030840775464867028 100 0.028411885802180615 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4899358.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.25485654081056 #Duplication Level Percentage of deduplicated Percentage of total 1 72.12653614172405 39.853414072965506 2 14.335692318449109 15.84233244938211 3 5.732861875088123 9.503053814288293 4 2.784615767767755 6.1545417907714555 5 1.546933891542401 4.273780512764659 6 0.9782385079045994 3.2431457050179135 7 0.6179632688329619 2.3901830236798967 8 0.42630760259053535 1.884445233871753 9 0.30720177575855323 1.5276951043759 >10 1.1257239513370343 9.490171516674291 >50 0.0150522735358417 0.5527778641665706 >100 0.0023869601651713393 0.21663345696661274 >500 6.0708162977493936E-5 0.023447401644881245 >1k 1.8212448893198852E-4 0.3166573269343053 >5k 6.07081629773295E-5 0.2819390712799615 >10k+ 1.8212448893198852E-4 4.445781655215907 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 68046 1.3888758486315962 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 53918 1.1005115364094642 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 10571 0.21576296322905983 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 8405 0.1715530892006667 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.041083750156653E-5 0.0 12-13 0.0 0.0 0.0 4.082167500313306E-5 0.0 14-15 0.0 0.0 0.0 4.082167500313306E-5 0.0 16-17 0.0 0.0 0.0 8.164335000626613E-5 0.0 18-19 0.0 0.0 0.0 1.1225960625861592E-4 0.0 20-21 0.0 0.0 0.0 2.5513546876958167E-4 0.0 22-23 0.0 0.0 0.0 6.93968475053262E-4 0.0 24-25 0.0 0.0 0.0 0.0015512236501190565 0.0 26-27 0.0 0.0 0.0 0.0028677226689700977 0.0 28-29 0.0 0.0 0.0 0.0072662581505576855 0.0 30-31 0.0 0.0 0.0 0.018696327151434943 0.0 32-33 0.0 0.0 0.0 0.04186262771571296 0.0 34-35 0.0 0.0 0.0 0.08643989681913426 0.0 36-37 0.0 0.0 0.0 0.15291799456173644 0.0 38-39 0.0 0.0 0.0 0.22953007312386642 0.0 40-41 0.0 0.0 0.0 0.3133573827428002 0.0 42-43 0.0 0.0 0.0 0.3919186962863298 0.0 44-45 0.0 0.0 0.0 0.4830837019870767 0.0 46-47 0.0 0.0 0.0 0.5991397240209839 0.0 48-49 0.0 0.0 0.0 0.725901230324463 0.0 50-51 0.0 0.0 0.0 0.8440493632022807 0.0 52-53 0.0 2.041083750156653E-5 0.0 0.9576764955735017 0.0 54-55 0.0 2.041083750156653E-5 0.0 1.077120716632669 0.0 56-57 0.0 2.041083750156653E-5 0.0 1.2115158761617337 0.0 58-59 0.0 2.041083750156653E-5 0.0 1.3827934190561293 0.0 60-61 0.0 2.041083750156653E-5 0.0 1.580298888140038 0.0 62-63 0.0 2.041083750156653E-5 0.0 1.7509539004906358 0.0 64-65 0.0 2.041083750156653E-5 0.0 1.914230395084417 0.0 66-67 0.0 2.041083750156653E-5 0.0 2.094253981848234 0.0 68-69 0.0 2.041083750156653E-5 0.0 2.286177086875464 0.0 70-71 2.041083750156653E-5 2.041083750156653E-5 0.0 2.5004500589669094 0.0 72-73 2.041083750156653E-5 2.041083750156653E-5 0.0 2.7222648355151837 0.0 74-75 2.041083750156653E-5 2.041083750156653E-5 0.0 2.937537938644206 0.0 76-77 2.041083750156653E-5 2.041083750156653E-5 0.0 3.1588016225799382 0.0 78-79 2.041083750156653E-5 2.041083750156653E-5 0.0 3.3916790730540614 0.0 80-81 2.041083750156653E-5 2.041083750156653E-5 0.0 3.637578637854184 0.0 82-83 2.041083750156653E-5 2.041083750156653E-5 0.0 3.8957349105739976 0.0 84-85 2.041083750156653E-5 2.041083750156653E-5 0.0 4.200427892797382 0.0 86-87 2.041083750156653E-5 2.041083750156653E-5 0.0 4.525511301684833 0.0 88 2.041083750156653E-5 2.041083750156653E-5 0.0 4.748560933901952 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 1890 0.0 23.882116 1 CATGGGG 9165 0.0 20.663359 4 GCATCGT 2020 0.0 20.471136 8 GTACCCG 650 0.0 18.807165 1 TATTCGC 425 0.0 18.796225 9 CTCGTCG 1420 0.0 18.531487 9 ATGGGGG 6350 0.0 18.20496 5 GCTATTG 2805 0.0 18.092623 8 CTGTGCG 2575 0.0 18.066273 9 TGATCGC 1655 0.0 17.603714 9 TACCTGG 3960 0.0 17.562845 2 GGGCTAT 3330 0.0 17.498344 6 CTATTGA 3010 0.0 17.48486 9 GTGATCG 1630 0.0 17.29714 8 CCGTATC 520 0.0 17.174711 94 GTATATA 2635 0.0 16.95143 1 ACGTTAA 555 0.0 16.933535 8 CTAGTAC 2895 0.0 16.719429 3 GTCCTAC 1775 0.0 16.688047 1 TCTAACG 1155 0.0 16.681313 2 >>END_MODULE