FastQCFastQC Report
Mon 16 Jan 2017
ERR1146540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4899358
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA679701.3873246249814772No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA526251.0741203235199388No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130610.2665859486079605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116680.2381536519682783No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT106830.21804897702923529No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG101140.2064352104908439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63570.12975169399745842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT150250.021.92710112-13
AGTACTT153100.019.968712-13
GTACTTT162950.019.91530814-15
CATGGGG89450.019.553794
GCTATTG28750.019.452818
CTATTGA29350.019.215469
GTACCCG6150.019.1309661
CTAACGC9400.019.0074583
GGCTATT30550.018.1528197
TAACGCA3401.022272E-917.9776424
CTGTGCG27300.017.9039299
GTCCTAC16400.017.7917981
GTGATCG17300.017.6579598
GGGCTAT31500.017.6058926
ACTTTTT186500.017.51375216-17
GGGCACC25000.017.48354594
CTCGTCG15500.017.2830669
TAGTACT31600.017.2599374
CGATAAT12800.017.25753866-67
CGCCTAC10450.017.0899417
Produced by FastQC (version 0.11.5)