##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146540_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4899358
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54313075304969	34.0	31.0	34.0	31.0	34.0
2	32.615574530377245	34.0	31.0	34.0	31.0	34.0
3	32.759302137137155	34.0	31.0	34.0	31.0	34.0
4	36.1843372131614	37.0	37.0	37.0	35.0	37.0
5	36.21440911237758	37.0	37.0	37.0	35.0	37.0
6	36.29780595743361	37.0	37.0	37.0	35.0	37.0
7	36.29136082727574	37.0	37.0	37.0	35.0	37.0
8	36.30623093882913	37.0	37.0	37.0	35.0	37.0
9	38.12981353883509	39.0	39.0	39.0	37.0	39.0
10-11	38.099244839834114	39.0	38.0	39.0	37.0	39.0
12-13	38.07994404573007	39.0	38.0	39.0	37.0	39.0
14-15	39.508966174751876	41.0	39.0	41.0	37.0	41.0
16-17	39.50015655112364	41.0	39.0	41.0	37.0	41.0
18-19	39.578328425887634	41.0	39.5	41.0	37.0	41.0
20-21	39.61078369859888	41.0	40.0	41.0	37.0	41.0
22-23	39.57967360213318	41.0	40.0	41.0	37.0	41.0
24-25	39.56458968705695	41.0	40.0	41.0	37.0	41.0
26-27	39.442103128613994	41.0	39.0	41.0	36.0	41.0
28-29	39.33842638157898	41.0	39.0	41.0	36.0	41.0
30-31	39.296705098912966	41.0	39.0	41.0	36.0	41.0
32-33	39.21165058768925	41.0	39.0	41.0	35.0	41.0
34-35	39.161718127150536	41.0	39.0	41.0	35.0	41.0
36-37	39.07384722651417	41.0	39.0	41.0	35.0	41.0
38-39	38.93977006783338	40.5	39.0	41.0	35.0	41.0
40-41	38.84168017523929	40.0	38.0	41.0	35.0	41.0
42-43	38.81221406968015	40.0	38.0	41.0	35.0	41.0
44-45	38.71370504053796	40.0	38.0	41.0	35.0	41.0
46-47	38.513358586982214	40.0	38.0	41.0	35.0	41.0
48-49	38.324438426422404	40.0	38.0	41.0	34.5	41.0
50-51	38.1724980089228	40.0	38.0	41.0	34.0	41.0
52-53	37.96477885469892	40.0	37.0	41.0	33.5	41.0
54-55	37.8439732307784	40.0	37.0	41.0	33.5	41.0
56-57	37.70876571991677	40.0	37.0	41.0	33.5	41.0
58-59	37.52321038797328	40.0	36.0	41.0	33.0	41.0
60-61	37.30819435934259	39.0	36.0	41.0	33.0	41.0
62-63	36.99221847025672	39.0	35.0	41.0	32.5	41.0
64-65	36.645487020952544	38.5	35.0	41.0	32.0	41.0
66-67	36.391478536575605	37.5	35.0	40.0	32.0	41.0
68-69	36.0940819184881	37.0	35.0	40.0	32.0	41.0
70-71	35.78283073006708	37.0	35.0	39.0	31.5	41.0
72-73	35.41121367330169	36.0	35.0	39.0	31.0	41.0
74-75	35.068143826191104	35.5	35.0	38.5	31.0	40.5
76-77	34.68242624850031	35.0	35.0	37.0	30.5	39.5
78-79	34.38531558624619	35.0	34.0	37.0	30.5	39.0
80-81	34.19078081250645	35.0	34.0	36.0	31.0	39.0
82-83	33.96193654352264	35.0	34.0	36.0	30.0	37.5
84-85	33.77180642851574	35.0	34.0	36.0	30.0	37.0
86-87	33.55339179133266	35.0	34.0	35.0	30.0	37.0
88-89	33.34272296492724	35.0	34.0	35.0	29.5	36.0
90-91	33.22703852219005	35.0	34.0	35.0	29.0	36.0
92-93	33.201521913687465	35.0	34.0	35.0	29.5	36.0
94-95	33.17078043694704	35.0	34.0	35.0	29.5	36.0
96-97	33.11590896194971	35.0	34.0	35.0	29.0	35.5
98-99	33.04620666626117	35.0	34.0	35.0	29.0	35.0
100	32.39890655061337	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.29862715295134734
1101	2	0.2429365240560415
1101	3	0.2501291338792555
1101	4	0.1735209657627763
1101	5	0.1580871268720827
1101	6	0.15465797926088243
1101	7	0.16313246483890254
1101	8	0.1789212885536955
1101	9	0.23018890733833075
1101	10-11	0.2080673094281309
1101	12-13	0.24121702061607664
1101	14-15	0.3288010123345302
1101	16-17	0.2991663284007302
1101	18-19	0.28164674622967567
1101	20-21	0.3015317615809039
1101	22-23	0.3035374071959325
1101	24-25	0.28555931660433487
1101	26-27	0.2731358081372832
1101	28-29	0.272215223218609
1101	30-31	0.31176036602895607
1101	32-33	0.3356340112939833
1101	34-35	0.30568437913883884
1101	36-37	0.35345970825158446
1101	38-39	0.36867666603473737
1101	40-41	0.34496873605719003
1101	42-43	0.33099921152111733
1101	44-45	0.374490280846949
1101	46-47	0.3781298675878091
1101	48-49	0.31763897971961086
1101	50-51	0.2961731981235829
1101	52-53	0.30528977230808607
1101	54-55	0.2721456802245541
1101	56-57	0.29059139717534066
1101	58-59	0.3242622569314264
1101	60-61	0.2613146858770108
1101	62-63	0.2923505575768175
1101	64-65	0.2969143593346999
1101	66-67	0.2761142632658178
1101	68-69	0.27646174824900527
1101	70-71	0.2544108575122479
1101	72-73	0.22231323016653448
1101	74-75	0.23755285575187912
1101	76-77	0.2362286038173309
1101	78-79	0.20099096879840062
1101	80-81	0.23291698895292257
1101	82-83	0.2070628754213928
1101	84-85	0.24195313603141955
1101	86-87	0.23927110012663633
1101	88-89	0.22313398508755
1101	90-91	0.26281085984181374
1101	92-93	0.25570425941695873
1101	94-95	0.30201706873357637
1101	96-97	0.25034757695867427
1101	98-99	0.219923565997874
1101	100	0.21728489796193884
1102	1	0.23292398694155025
1102	2	0.26017199170468075
1102	3	0.2723975295194805
1102	4	0.19447382296537086
1102	5	0.18034121865850494
1102	6	0.142807470449398
1102	7	0.16469933499132594
1102	8	0.18749148156937423
1102	9	0.2043898524537724
1102	10-11	0.22696506693755225
1102	12-13	0.2257624867937551
1102	14-15	0.30295530087957445
1102	16-17	0.3466991268774251
1102	18-19	0.2885819827488376
1102	20-21	0.2946366018593949
1102	22-23	0.31702386077019895
1102	24-25	0.3173765489303335
1102	26-27	0.34751236296619226
1102	28-29	0.39346927797105735
1102	30-31	0.39202079811561674
1102	32-33	0.3861152501512848
1102	34-35	0.41666842290384665
1102	36-37	0.4294941577571123
1102	38-39	0.39434433352037956
1102	40-41	0.3931090322083435
1102	42-43	0.3772348325070709
1102	44-45	0.42124375335452413
1102	46-47	0.4779809878760375
1102	48-49	0.46488170644401805
1102	50-51	0.44002549538492275
1102	52-53	0.48878691684275566
1102	54-55	0.5046382052906253
1102	56-57	0.47076089316163205
1102	58-59	0.5477371124208972
1102	60-61	0.5019622281004246
1102	62-63	0.49945805930835974
1102	64-65	0.5089136230243199
1102	66-67	0.44789408087140004
1102	68-69	0.4209690319794248
1102	70-71	0.4170588087738665
1102	72-73	0.41739753656070633
1102	74-75	0.4474048249088227
1102	76-77	0.3916389610974278
1102	78-79	0.3689594595410455
1102	80-81	0.4236256424411806
1102	82-83	0.43291742405730815
1102	84-85	0.42794214193335023
1102	86-87	0.3799707759961066
1102	88-89	0.4634333455024304
1102	90-91	0.4090040738022864
1102	92-93	0.3495153043639334
1102	94-95	0.3674146524121582
1102	96-97	0.3293107275265541
1102	98-99	0.3732029351386217
1102	100	0.41953234027683806
1103	1	0.1813253426592354
1103	2	0.21691911600051128
1103	3	0.2008488885441153
1103	4	0.16123059767965486
1103	5	0.13425206390369482
1103	6	0.1182629913689155
1103	7	0.1046649305608014
1103	8	0.08973336554011269
1103	9	0.13747851202705874
1103	10-11	0.18039672099881443
1103	12-13	0.156666622526771
1103	14-15	0.22896673370843246
1103	16-17	0.2542310161524526
1103	18-19	0.24801100269556997
1103	20-21	0.27169552491604776
1103	22-23	0.2559690116932103
1103	24-25	0.2611742306624052
1103	26-27	0.2925570635059458
1103	28-29	0.337267731999944
1103	30-31	0.33696998706215453
1103	32-33	0.32867109126252814
1103	34-35	0.33374852157957946
1103	36-37	0.34964226251033637
1103	38-39	0.3821302694534765
1103	40-41	0.3856781315036315
1103	42-43	0.38256545302755285
1103	44-45	0.33666714875216286
1103	46-47	0.39757484938746757
1103	48-49	0.3541108448332224
1103	50-51	0.3714072523870229
1103	52-53	0.3721146314002368
1103	54-55	0.31672736237499066
1103	56-57	0.3887962090543269
1103	58-59	0.4210735091138673
1103	60-61	0.4052179099007631
1103	62-63	0.42975494185250085
1103	64-65	0.3998594253100549
1103	66-67	0.3501292040798347
1103	68-69	0.36349253790919533
1103	70-71	0.3593905065597838
1103	72-73	0.3684494276142871
1103	74-75	0.3669653637116923
1103	76-77	0.37502360279272295
1103	78-79	0.34485734194875306
1103	80-81	0.3490372665160635
1103	82-83	0.3225275326680688
1103	84-85	0.3349167114399094
1103	86-87	0.3737093732235124
1103	88-89	0.33616355249722574
1103	90-91	0.31541032147590187
1103	92-93	0.28239201277311565
1103	94-95	0.25917532912446006
1103	96-97	0.2959503663367542
1103	98-99	0.35217407777430765
1103	100	0.3588876441125848
1104	1	0.16935490360165772
1104	2	0.16824083343894358
1104	3	0.2015499506917493
1104	4	0.1406636844278708
1104	5	0.14935662704682073
1104	6	0.139437721584045
1104	7	0.16742786280033073
1104	8	0.12216300195683516
1104	9	0.15471369222171916
1104	10-11	0.15604303819056042
1104	12-13	0.1665606565747595
1104	14-15	0.25647026244850224
1104	16-17	0.2516283660109977
1104	18-19	0.21413136825351842
1104	20-21	0.23536328245693738
1104	22-23	0.22891082903524307
1104	24-25	0.20361158235093058
1104	26-27	0.22343967261102193
1104	28-29	0.2689014015565334
1104	30-31	0.27936628726320833
1104	32-33	0.21198628663704255
1104	34-35	0.24666523873438706
1104	36-37	0.24721145970257652
1104	38-39	0.24704787128081307
1104	40-41	0.22999983144973868
1104	42-43	0.27113471945730794
1104	44-45	0.26130890488521885
1104	46-47	0.1672468390322024
1104	48-49	0.18397294323182223
1104	50-51	0.21951516713977526
1104	52-53	0.23319854599925804
1104	54-55	0.20397326718222786
1104	56-57	0.20087125740548828
1104	58-59	0.181221766124132
1104	60-61	0.21101703800872684
1104	62-63	0.23693485409201998
1104	64-65	0.22069612166669828
1104	66-67	0.23134888508356966
1104	68-69	0.1956469806397365
1104	70-71	0.15936052410192048
1104	72-73	0.19356223009074114
1104	74-75	0.17965308013642556
1104	76-77	0.1631911878536414
1104	78-79	0.14449979742069274
1104	80-81	0.15486035509542262
1104	82-83	0.19549665550495376
1104	84-85	0.14994547214980258
1104	86-87	0.1672773416566926
1104	88-89	0.20291435464777408
1104	90-91	0.13682179767299374
1104	92-93	0.11047214848711917
1104	94-95	0.1319137232445584
1104	96-97	0.12722353584703683
1104	98-99	0.12072792782429076
1104	100	0.22519654671189926
1105	1	0.19026271976914444
1105	2	0.15800466275746317
1105	3	0.1889465713111207
1105	4	0.12494603746364419
1105	5	0.11293935277299028
1105	6	0.09331366366156857
1105	7	0.13581630317954563
1105	8	0.11601979188304767
1105	9	0.12603631070440713
1105	10-11	0.12825183588286393
1105	12-13	0.12625660033125996
1105	14-15	0.1980006467688895
1105	16-17	0.20505062112896155
1105	18-19	0.1691540315160367
1105	20-21	0.16782024441427268
1105	22-23	0.21156895719103375
1105	24-25	0.18175025553327373
1105	26-27	0.20612541943981455
1105	28-29	0.19596315188381652
1105	30-31	0.190153778224321
1105	32-33	0.15834140622349224
1105	34-35	0.1265601295814136
1105	36-37	0.17224068108892965
1105	38-39	0.16610635727322887
1105	40-41	0.19931615217577558
1105	42-43	0.1840056940630319
1105	44-45	0.17831737083880483
1105	46-47	0.1475122374365867
1105	48-49	0.2031904376159872
1105	50-51	0.17782755522023308
1105	52-53	0.16320061434070965
1105	54-55	0.08485626849643069
1105	56-57	0.15764571803399718
1105	58-59	0.139636413215392
1105	60-61	0.14526127115768617
1105	62-63	0.11763583218198193
1105	64-65	0.11910404500950023
1105	66-67	0.11633016753305725
1105	68-69	0.13225925279606088
1105	70-71	0.09527462723205815
1105	72-73	0.08303464963376683
1105	74-75	0.11975841522063035
1105	76-77	0.1037108685835264
1105	78-79	0.09648146779891675
1105	80-81	0.10295748315110131
1105	82-83	0.060583108725019486
1105	84-85	0.05078587078688912
1105	86-87	0.07593445840969792
1105	88-89	0.08179604005456298
1105	90-91	0.07803146452318543
1105	92-93	0.06636705052145686
1105	94-95	0.07395482358823102
1105	96-97	0.072978812379219
1105	98-99	0.015634299430303145
1105	100	0.05619869506883646
1106	1	0.12143021281129052
1106	2	0.1189909301979526
1106	3	0.1305403682463151
1106	4	0.08977230976804407
1106	5	0.01928765248204911
1106	6	0.05251683093385395
1106	7	0.09682671013832334
1106	8	0.09526899717367598
1106	9	0.06746238718769604
1106	10-11	0.07605558147352554
1106	12-13	0.0736239712647091
1106	14-15	0.17654244693044063
1106	16-17	0.15007868850070594
1106	18-19	0.14616621738773716
1106	20-21	0.1444757809415549
1106	22-23	0.17567042632672525
1106	24-25	0.14187542469309022
1106	26-27	0.16375102836597222
1106	28-29	0.18013740512164134
1106	30-31	0.2221770161431209
1106	32-33	0.1949159796857387
1106	34-35	0.19919870371094106
1106	36-37	0.1889221350424677
1106	38-39	0.22901045605456716
1106	40-41	0.25451987023943445
1106	42-43	0.224726360643686
1106	44-45	0.1760271942325815
1106	46-47	0.15923438123852662
1106	48-49	0.13385974166634185
1106	50-51	0.0808618110181456
1106	52-53	0.12611952971970197
1106	54-55	0.09901465078608851
1106	56-57	0.1340238489954757
1106	58-59	0.15206056058897133
1106	60-61	0.13109414797568064
1106	62-63	0.16170664726987383
1106	64-65	0.14349249554176424
1106	66-67	0.08995271909395797
1106	68-69	0.08824710113422896
1106	70-71	0.07260552592853031
1106	72-73	0.05841381698242998
1106	74-75	0.03538484241992279
1106	76-77	0.026738099425699602
1106	78-79	0.011615683311255509
1106	80-81	0.04136894624416243
1106	82-83	0.07991082334994104
1106	84-85	0.059635547997764604
1106	86-87	0.06101397498789396
1106	88-89	0.06421396014426506
1106	90-91	0.039216448562008566
1106	92-93	0.04356646944786746
1106	94-95	0.04234848959637105
1106	96-97	-0.010334317454422148
1106	98-99	0.04455546269463895
1106	100	0.02303369771371422
1107	1	0.08364084147756046
1107	2	0.08648486767144448
1107	3	0.12594590834886787
1107	4	0.08600136951999815
1107	5	0.04427418989511267
1107	6	0.06778805008574551
1107	7	0.061118004722978014
1107	8	0.09155084434569716
1107	9	0.06833867431480911
1107	10-11	0.09413322875715835
1107	12-13	0.09011553306498854
1107	14-15	0.1646628444462408
1107	16-17	0.1429086532009407
1107	18-19	0.13506026868821408
1107	20-21	0.15648654431046083
1107	22-23	0.14787970429712516
1107	24-25	0.16697629164121253
1107	26-27	0.12476963253137541
1107	28-29	0.166444992492643
1107	30-31	0.16687288053493177
1107	32-33	0.17437851241339075
1107	34-35	0.1151514489389669
1107	36-37	0.12319557503510481
1107	38-39	0.12309315577433466
1107	40-41	0.1273007021560133
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2216	44-45	-0.4761488874264401
2216	46-47	-0.4489196158543365
2216	48-49	-0.47884486388785064
2216	50-51	-0.45887380090387353
2216	52-53	-0.48598838666474364
2216	54-55	-0.49133996315890727
2216	56-57	-0.47655015936622647
2216	58-59	-0.4405512580251525
2216	60-61	-0.45403869773698347
2216	62-63	-0.47017466737492697
2216	64-65	-0.48670146735097575
2216	66-67	-0.43311822098952035
2216	68-69	-0.4662758152961288
2216	70-71	-0.45214859883332537
2216	72-73	-0.43675299626981
2216	74-75	-0.45269315977252944
2216	76-77	-0.4677317912529446
2216	78-79	-0.49424649481920113
2216	80-81	-0.47029010101400104
2216	82-83	-0.48262057506572376
2216	84-85	-0.4853142434606923
2216	86-87	-0.4855107168693067
2216	88-89	-0.494951471458414
2216	90-91	-0.5095072132959686
2216	92-93	-0.5314232269220014
2216	94-95	-0.5780885870841175
2216	96-97	-0.5693447920613011
2216	98-99	-0.5882018021473243
2216	100	-0.6821022153826277
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	23.0
8	101.0
9	206.0
10	411.0
11	404.0
12	305.0
13	294.0
14	446.0
15	773.0
16	1402.0
17	2605.0
18	4643.0
19	7596.0
20	11000.0
21	15980.0
22	23742.0
23	29311.0
24	29181.0
25	25837.0
26	23599.0
27	25445.0
28	30880.0
29	38691.0
30	47137.0
31	60788.0
32	78979.0
33	106217.0
34	157735.0
35	255513.0
36	487209.0
37	1210716.0
38	1625339.0
39	594413.0
40	2437.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.562280723956924	18.816017551064732	13.08495724499885	23.53674447997949
2	14.33434422431483	21.41303273014931	39.88220300684905	24.370420038686806
3	19.32720298130074	26.993075391001803	30.76913656498082	22.91058506271664
4	12.080705267914695	17.166841043255054	39.56104044652381	31.191413242306442
5	12.803602596993152	38.8074912258778	35.157195025234024	13.231711151895023
6	28.566961278602193	38.10601947296442	18.857708558714865	14.46931068971852
7	25.47750653810286	34.14454182457004	22.4891960312343	17.8887556060928
8	24.317063582616335	33.728582397938666	21.588052965306883	20.366301054138113
9	26.051739840199474	16.61380940115011	21.212330268578047	36.122120490072376
10-11	23.720311926583033	25.382886900691886	29.01080100698908	21.886000165736
12-13	27.35241025456805	23.24092258618374	27.723979345865313	21.6826878133829
14-15	24.189403668563784	24.56453460413981	27.29034183268168	23.95571989461472
16-17	21.293044925477993	27.488081091440957	28.950946226015734	22.267927757065316
18-19	20.877541506458602	28.59257274116323	29.91554199550227	20.6143437568759
20-21	22.0156600109647	27.26801144149907	28.29303349540899	22.423295052127237
22-23	21.650128849342863	26.70556861233886	27.413298542156767	24.231003996161512
24-25	22.286185251210462	26.823279703177437	28.456483482121538	22.434051563490563
26-27	23.468574862257462	25.59032224222031	28.84172783454485	22.099375060977376
28-29	21.832636966229536	25.86415703045023	30.278155973780635	22.0250500295396
30-31	21.954236587074146	27.66620800042781	29.111113809881534	21.268441602616512
32-33	21.69607333356126	27.493921272764293	28.125425128195445	22.684580265479003
34-35	22.08580185257966	26.47757275997793	28.574882434171656	22.861742953270753
36-37	23.3577986932032	26.109819999069256	28.93425969545087	21.59812161227667
38-39	24.207970425235217	24.76959009754839	29.54652612448442	21.475913352731975
40-41	22.19137405128607	25.663181245182948	30.543199958197803	21.602244745333177
42-43	21.96170877165517	27.055353900607237	28.962444894143996	22.020492433593596
44-45	22.08313496406901	27.75258211722131	27.791944702558364	22.372338216151313
46-47	21.963984306854613	26.677122504241137	27.00098268408386	24.3579105048204
48-49	23.040643305798554	26.526546498252767	27.515204410572615	22.917605785376068
50-51	23.932234518401934	27.012975402549156	27.166956316386283	21.887833762662627
52-53	22.032835370023005	27.09256851502973	28.37359086724811	22.501005247699158
54-55	21.50494222142936	26.85510243425314	28.576054630240925	23.06390071407657
56-57	22.93523030968369	27.815238302653306	26.612884213895217	22.636647173767795
58-59	21.865972078562454	27.05368548667178	27.736652670874218	23.34368976389155
60-61	22.349875639039595	26.2160824118566	28.045567823541845	23.388474125561963
62-63	24.316587966576012	25.898294969144033	27.018027387815245	22.767089676464707
64-65	23.12350828075766	26.33777351035255	28.305378364194944	22.233339844694843
66-67	22.355596515292543	26.27503510834599	28.649594212838224	22.719774163523248
68-69	21.90356840363071	27.8616001158568	27.845229934203914	22.38960154630858
70-71	21.939682666038	27.369477604247994	26.729757891761096	23.961081837952914
72-73	22.192706627026773	26.718890491222375	26.46920202771731	24.619200854033544
74-75	22.897962986908873	26.959905461315447	26.99808548753832	23.144046064237358
76-77	23.55517304162666	25.797380289877793	27.48880013912566	23.158646529369886
78-79	22.381963651386172	25.98798421776633	28.5124843689612	23.1175677618863
80-81	21.828722538856905	27.92781171478814	27.712814004375474	22.53065174197948
82-83	22.058038160403264	27.248578407054573	27.12558238646366	23.5678010460785
84-85	22.62521558982314	26.40505373161746	26.622735669017317	24.346995009542084
86-87	23.012751371073197	26.476737603548056	27.533774595454442	22.9767364299243
88-89	24.037238847797237	24.948921754046356	27.973070283258217	23.040769114898193
90-91	22.494018604070163	25.782969931978844	28.81954125418065	22.90347020977034
92-93	21.882724226317	27.2984235893764	27.812929775697143	23.005922408609454
94-95	21.440239868186797	27.742147822444064	27.26483013333891	23.552782176030224
96-97	21.833726583580646	26.580968048757587	26.314146395228743	25.27115897243302
98-99	22.323212252454596	26.521379409443558	26.771852509991927	24.383555828109913
100	22.057512726732394	28.0190997427609	27.159207181875573	22.76418034863113
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	714.0
1	440.0
2	405.0
3	639.0
4	431.5
5	313.5
6	589.5
7	1403.5
8	2614.0
9	3171.5
10	2365.5
11	1569.0
12	1613.0
13	1831.5
14	2167.5
15	2596.5
16	3132.0
17	3794.5
18	4583.5
19	5481.0
20	6518.0
21	7831.5
22	9769.5
23	12067.5
24	14577.0
25	17814.5
26	22410.5
27	28011.0
28	33468.0
29	40974.0
30	51140.0
31	59650.0
32	70218.5
33	81451.0
34	90286.0
35	100312.0
36	110264.5
37	121831.0
38	127862.0
39	127668.5
40	127386.5
41	127024.5
42	130123.0
43	136495.0
44	147766.5
45	164644.0
46	181094.0
47	209079.5
48	385526.5
49	393280.0
50	242235.5
51	212184.0
52	171570.5
53	155093.5
54	147145.0
55	132880.5
56	119894.5
57	104423.5
58	88865.0
59	80708.5
60	67648.0
61	51726.0
62	39727.0
63	30958.5
64	22109.5
65	14585.0
66	10686.0
67	7856.0
68	5821.0
69	4290.0
70	3128.0
71	2403.5
72	1872.5
73	1703.5
74	1326.0
75	921.0
76	610.5
77	344.5
78	240.5
79	159.5
80	91.0
81	49.0
82	25.0
83	9.0
84	9.5
85	6.0
86	2.5
87	1.5
88	2.0
89	2.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005755856175441762
2	0.09523696778230945
3	4.0821675003133065E-4
4	0.0
5	0.0408624966781362
6	0.005265996075404165
7	2.0410837501566534E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	4.082167500313307E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.429413812993458E-4
24-25	0.0
26-27	0.0
28-29	5.102709375391633E-5
30-31	1.5308128126174898E-4
32-33	0.002051289168907436
34-35	0.0016634832563776722
36-37	0.0010205418750783265
38-39	9.388985250720603E-4
40-41	0.0019186187251472538
42-43	7.65406406308745E-4
44-45	7.245847313056119E-4
46-47	4.388330062836804E-4
48-49	8.062280813118781E-4
50-51	0.0010103364563275434
52-53	0.0013879369501065243
54-55	0.001908413306396471
56-57	0.001071568968832243
58-59	0.0030105985314810633
60-61	0.002275808381424668
62-63	0.0012756773438479083
64-65	0.004674081787858736
66-67	0.004857779325372835
68-69	0.004214837944073489
70-71	0.002102316262661353
72-73	0.005163941887896333
74-75	0.004071962081562523
76-77	0.0038780591252976413
78-79	0.0010511581313306765
80-81	7.143793125548286E-5
82-83	2.551354687695816E-4
84-85	1.6328670001253227E-4
86-87	5.102709375391633E-5
88-89	2.449300500187984E-4
90-91	0.0
92-93	0.0
94-95	1.0205418750783267E-5
96-97	3.673950750281976E-4
98-99	6.021197062962126E-4
100	5.102709375391632E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4899358.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.396583137443855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.29702640754596	35.61630378933078
2	15.82078896137778	16.26268990306814
3	6.259082097121663	9.650863001063998
4	3.0096572057896562	6.187443871703001
5	1.7370448251480475	4.463908438459412
6	1.0649465761312067	3.284076914223824
7	0.695831544717192	2.50343546863992
8	0.4754210583767417	1.9548014361721402
9	0.3362357110501205	1.555323000908856
>10	1.273348100101408	10.072136205375402
>50	0.019528029105244028	0.6633907768406287
>100	0.00878907050344321	0.8145539407447696
>500	9.201652127356981E-4	0.3452076583677982
>1k	9.201652125235912E-4	0.7993927024425138
>5k	6.572608660882794E-5	0.21474558018200898
>10k+	3.943565196529677E-4	5.611727312476879
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	67970	1.3873246249814772	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	52625	1.0741203235199388	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13061	0.2665859486079605	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11668	0.2381536519682783	No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT	10683	0.21804897702923529	No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG	10114	0.2064352104908439	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6357	0.12975169399745842	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.041083750156653E-5	0.0
12-13	0.0	0.0	0.0	4.082167500313306E-5	0.0
14-15	0.0	0.0	0.0	4.082167500313306E-5	0.0
16-17	0.0	0.0	0.0	8.164335000626613E-5	0.0
18-19	0.0	0.0	0.0	1.1225960625861592E-4	0.0
20-21	0.0	0.0	0.0	2.5513546876958167E-4	0.0
22-23	0.0	0.0	0.0	6.735576375516955E-4	0.0
24-25	0.0	0.0	0.0	0.00153081281261749	0.0
26-27	0.0	0.0	0.0	0.0028677226689700977	0.0
28-29	0.0	0.0	0.0	0.007296874406810035	0.0
30-31	0.0	0.0	0.0	0.018737148826438076	0.0
32-33	0.0	0.0	0.0	0.04185242229696218	0.0
34-35	0.0	0.0	0.0	0.08656236184414365	0.0
36-37	0.0	0.0	0.0	0.15327518421801387	0.0
38-39	0.0	0.0	0.0	0.23023424701767048	0.0
40-41	0.0	0.0	0.0	0.31407176205535503	0.0
42-43	0.0	0.0	0.0	0.39267389727388774	0.0
44-45	0.0	0.0	0.0	0.48428794139966913	0.0
46-47	0.0	0.0	0.0	0.6008338235336139	0.0
48-49	0.0	0.0	0.0	0.7281260116121338	0.0
50-51	0.0	0.0	0.0	0.8470191400587588	0.0
52-53	0.0	0.0	0.0	0.9611565433675188	0.0
54-55	0.0	0.0	0.0	1.080876310732957	0.0
56-57	0.0	0.0	0.0	1.2149653076994986	0.0
58-59	0.0	0.0	0.0	1.3868347648814396	0.0
60-61	0.0	0.0	0.0	1.5849627645091457	0.0
62-63	0.0	0.0	0.0	1.7563321561722987	0.0
64-65	0.0	0.0	0.0	1.9202311813098776	0.0
66-67	0.0	0.0	0.0	2.101122228667511	0.0
68-69	0.0	0.0	0.0	2.2937495075885455	0.0
70-71	0.0	0.0	0.0	2.509094048648823	0.0
72-73	0.0	0.0	0.0	2.7319089562346743	0.0
74-75	0.0	0.0	0.0	2.948223012076276	0.0
76-77	0.0	0.0	0.0	3.171027714243376	0.0
78-79	0.0	0.0	0.0	3.406017686398912	2.041083750156653E-5
80-81	0.0	0.0	0.0	3.6536215561304157	2.041083750156653E-5
82-83	0.0	0.0	0.0	3.9134923392003604	2.041083750156653E-5
84-85	0.0	0.0	0.0	4.219899831773877	2.041083750156653E-5
86-87	0.0	0.0	0.0	4.546391588448936	2.041083750156653E-5
88	0.0	0.0	0.0	4.770380119191127	2.041083750156653E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACT	15025	0.0	21.927101	12-13
AGTACTT	15310	0.0	19.9687	12-13
GTACTTT	16295	0.0	19.915308	14-15
CATGGGG	8945	0.0	19.55379	4
GCTATTG	2875	0.0	19.45281	8
CTATTGA	2935	0.0	19.21546	9
GTACCCG	615	0.0	19.130966	1
CTAACGC	940	0.0	19.007458	3
GGCTATT	3055	0.0	18.152819	7
TAACGCA	340	1.022272E-9	17.977642	4
CTGTGCG	2730	0.0	17.903929	9
GTCCTAC	1640	0.0	17.791798	1
GTGATCG	1730	0.0	17.657959	8
GGGCTAT	3150	0.0	17.605892	6
ACTTTTT	18650	0.0	17.513752	16-17
GGGCACC	2500	0.0	17.483545	94
CTCGTCG	1550	0.0	17.283066	9
TAGTACT	3160	0.0	17.259937	4
CGATAAT	1280	0.0	17.257538	66-67
CGCCTAC	1045	0.0	17.089941	7
>>END_MODULE