FastQCFastQC Report
Mon 16 Jan 2017
ERR1146539_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146539_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2965267
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA553911.867993674768579No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG134730.4543604336472905No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC108870.3671507489882024No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA100360.3384518156375126No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA50170.16919218404278602No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC46080.15539915967095036No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43110.14538319820778364No Hit
ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT30190.1018120796542099No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGC2300.034.7349
GGGCACC14950.032.06972594
TATTCTC10250.029.3425035
AGGGCTA19850.028.409385
CGTTAAC6750.027.1643731
GTACTAG19450.026.8313331
TAACCAC6500.026.748546
GCTATTG21100.026.5031938
CTATTGA21500.025.7915389
CATGGGG64900.025.2713874
ATGGGGG53150.024.4032045
TACCCCG1351.4599302E-524.3671825
GGGCTAT24550.024.3088266
GGGTAAA2803.6379788E-1223.507631
TAGGCAT23950.023.3497645
GTGATCG10500.023.2727538
CTTTGCG9300.023.2442722
TCGCAAT2253.1723175E-922.9852391
TGGGGGG52200.022.9552176
GCATCGT20150.022.8551868
Produced by FastQC (version 0.11.5)