##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146539_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2965267
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.636733218290292	31.0	31.0	34.0	30.0	34.0
2	31.86359339647998	33.0	31.0	34.0	30.0	34.0
3	31.98418220011891	34.0	31.0	34.0	30.0	34.0
4	35.36655350091577	37.0	35.0	37.0	33.0	37.0
5	35.38161217859977	37.0	35.0	37.0	33.0	37.0
6	35.50656450161149	37.0	35.0	37.0	33.0	37.0
7	35.47266401305515	37.0	36.0	37.0	33.0	37.0
8	35.412163896202266	37.0	36.0	37.0	33.0	37.0
9	37.036429771754115	39.0	38.0	39.0	34.0	39.0
10-11	37.02131713602856	39.0	38.0	39.0	34.0	39.0
12-13	36.99315559104795	39.0	38.0	39.0	34.0	39.0
14-15	38.32457448182575	40.0	39.0	41.0	34.0	41.0
16-17	38.37598772724345	40.5	39.0	41.0	34.0	41.0
18-19	38.50621647224348	41.0	39.0	41.0	34.0	41.0
20-21	38.571087527699866	41.0	39.0	41.0	34.0	41.0
22-23	38.59207079834632	41.0	39.0	41.0	34.0	41.0
24-25	38.61275864871527	41.0	39.0	41.0	34.0	41.0
26-27	38.52049967169904	40.5	38.5	41.0	34.0	41.0
28-29	38.46044639487776	40.0	38.0	41.0	34.0	41.0
30-31	38.49087316589029	40.0	38.0	41.0	34.0	41.0
32-33	38.48195423885943	40.0	38.0	41.0	34.0	41.0
34-35	38.374464255663995	40.0	38.0	41.0	33.0	41.0
36-37	38.191981194273566	40.0	38.0	41.0	33.0	41.0
38-39	38.05921355479962	40.0	38.0	41.0	33.0	41.0
40-41	37.98877639011934	40.0	38.0	41.0	33.0	41.0
42-43	37.89305111479	40.0	38.0	41.0	32.0	41.0
44-45	37.7877976924169	40.0	37.0	41.0	32.0	41.0
46-47	37.61537359030401	40.0	37.0	41.0	32.0	41.0
48-49	37.410424761075475	40.0	36.5	41.0	31.0	41.0
50-51	37.16719978335846	39.5	36.0	41.0	31.0	41.0
52-53	36.92776653839266	39.0	35.0	41.0	30.0	41.0
54-55	36.7497778446258	39.0	35.0	41.0	30.0	41.0
56-57	36.539861503196846	39.0	35.0	40.5	30.0	41.0
58-59	36.2802315946591	38.0	35.0	40.0	30.0	41.0
60-61	35.92897722194999	38.0	35.0	40.0	29.0	41.0
62-63	35.605601788978866	37.0	35.0	40.0	29.0	41.0
64-65	35.19328192031274	36.5	34.0	39.5	28.0	41.0
66-67	34.8594934284164	36.0	34.0	39.0	28.0	41.0
68-69	34.58632005144899	35.5	34.0	39.0	28.0	40.5
70-71	34.20726598987545	35.0	33.5	37.5	27.5	40.0
72-73	33.78389231054067	35.0	33.0	37.0	26.5	39.5
74-75	33.45886323221484	35.0	33.0	36.5	26.0	39.0
76-77	33.101036601425776	35.0	32.5	36.0	26.0	39.0
78-79	32.843280217262055	35.0	32.0	36.0	26.0	37.5
80-81	32.64807014005821	35.0	32.0	35.0	26.0	37.0
82-83	32.427369103692854	35.0	32.0	35.0	25.5	37.0
84-85	32.2777176557794	35.0	32.0	35.0	25.0	36.0
86-87	32.10753820819508	35.0	32.0	35.0	25.0	36.0
88-89	31.87546382838375	35.0	31.5	35.0	24.5	36.0
90-91	31.61772295715698	35.0	31.0	35.0	24.0	35.5
92-93	31.59551028625753	35.0	31.0	35.0	24.0	35.0
94-95	31.61377390299086	35.0	31.0	35.0	24.0	35.0
96-97	31.479689181446393	35.0	31.0	35.0	24.0	35.0
98-99	31.379701389453295	35.0	31.0	35.0	23.5	35.0
100	30.076380980195037	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.36935952783199255
1101	2	0.3910878439035166
1101	3	0.4429213303558299
1101	4	0.3281280147721688
1101	5	0.2940245610479977
1101	6	0.38305583586543435
1101	7	0.33726574977994517
1101	8	0.4015748634403522
1101	9	0.48213590065462597
1101	10-11	0.5067405146920052
1101	12-13	0.5015453760115349
1101	14-15	0.6208073109250165
1101	16-17	0.6241189831217255
1101	18-19	0.5206924160777717
1101	20-21	0.49464329140537444
1101	22-23	0.44936600303854846
1101	24-25	0.5175769253309213
1101	26-27	0.4942074554889828
1101	28-29	0.5824345552589989
1101	30-31	0.5116079887383478
1101	32-33	0.4889733241588061
1101	34-35	0.4932972302577028
1101	36-37	0.6072896613911496
1101	38-39	0.6227259060233195
1101	40-41	0.5394013040436363
1101	42-43	0.5583434633715001
1101	44-45	0.532173188285725
1101	46-47	0.539998223948345
1101	48-49	0.5416978287948453
1101	50-51	0.5538150443499248
1101	52-53	0.5287348982561895
1101	54-55	0.5945867882438662
1101	56-57	0.6134609388208574
1101	58-59	0.5502305303489479
1101	60-61	0.6199041556354388
1101	62-63	0.6023483722506882
1101	64-65	0.5215823830587354
1101	66-67	0.3887330830571045
1101	68-69	0.41531534096288425
1101	70-71	0.4180930708782924
1101	72-73	0.49166279236805366
1101	74-75	0.5355095932995155
1101	76-77	0.5631430638750246
1101	78-79	0.4957691828595472
1101	80-81	0.42994096984533314
1101	82-83	0.42324043075559103
1101	84-85	0.4997520011300196
1101	86-87	0.513477840878835
1101	88-89	0.4808311935843079
1101	90-91	0.46339592032331467
1101	92-93	0.4707090094866544
1101	94-95	0.44252961277869574
1101	96-97	0.45681923953240045
1101	98-99	0.37441492546610533
1101	100	0.21182599896841836
1102	1	0.13670046662271318
1102	2	0.20177795784697494
1102	3	0.21745250592609722
1102	4	0.24938185988796135
1102	5	0.18973599541603647
1102	6	0.2108131025143365
1102	7	0.15223926793257903
1102	8	0.21137355065121
1102	9	0.31763429225148343
1102	10-11	0.24701274658348638
1102	12-13	0.2523991733769577
1102	14-15	0.31131520934360424
1102	16-17	0.2961787598945165
1102	18-19	0.2798781627078526
1102	20-21	0.28115783450700604
1102	22-23	0.28719013462195875
1102	24-25	0.24730341894286312
1102	26-27	0.32876475247206116
1102	28-29	0.3926806859220022
1102	30-31	0.31472926544942936
1102	32-33	0.33912453366182405
1102	34-35	0.3528908878783241
1102	36-37	0.4263426498837717
1102	38-39	0.310654606878785
1102	40-41	0.3517139501638056
1102	42-43	0.35297938987284283
1102	44-45	0.3368041811589251
1102	46-47	0.3315666361562961
1102	48-49	0.35927029632401286
1102	50-51	0.3020476895704505
1102	52-53	0.2729680175202276
1102	54-55	0.33276878808469235
1102	56-57	0.294926903995389
1102	58-59	0.3698109787641357
1102	60-61	0.340560313760669
1102	62-63	0.29064687895593266
1102	64-65	0.3168594505401572
1102	66-67	0.30376164677443285
1102	68-69	0.32624374539698664
1102	70-71	0.3444386307283125
1102	72-73	0.3870647576302346
1102	74-75	0.300900869514642
1102	76-77	0.2732024461882432
1102	78-79	0.20724709139336994
1102	80-81	0.26680987778054543
1102	82-83	0.32342031643151614
1102	84-85	0.3454058524741299
1102	86-87	0.27293931994076104
1102	88-89	0.30758328035618376
1102	90-91	0.41033865678856074
1102	92-93	0.3737734528776713
1102	94-95	0.3728079461062599
1102	96-97	0.41597638207647947
1102	98-99	0.30867115867706474
1102	100	0.27762808406788153
1103	1	0.20808754167110521
1103	2	0.25618759736847707
1103	3	0.34646061022489505
1103	4	0.23035995107358787
1103	5	0.22014216931278696
1103	6	0.21322331740983458
1103	7	0.25161338145520773
1103	8	0.24325551423761738
1103	9	0.327131308335197
1103	10-11	0.3121983827508714
1103	12-13	0.3122196700718973
1103	14-15	0.39626706079899066
1103	16-17	0.3838168050502233
1103	18-19	0.2972573007173267
1103	20-21	0.3783112943334075
1103	22-23	0.37386615669134216
1103	24-25	0.3251846376652736
1103	26-27	0.4307991344133484
1103	28-29	0.39331515840663656
1103	30-31	0.36001850238754685
1103	32-33	0.4061238244799341
1103	34-35	0.4543805601381692
1103	36-37	0.4439470312006222
1103	38-39	0.4660365280926939
1103	40-41	0.479081792555931
1103	42-43	0.5017584484896176
1103	44-45	0.5195913552251668
1103	46-47	0.5533563057399675
1103	48-49	0.4998131995987478
1103	50-51	0.5330160239296404
1103	52-53	0.51084362746127
1103	54-55	0.47066257359954733
1103	56-57	0.4714471290602873
1103	58-59	0.49570191640073347
1103	60-61	0.47330946408047225
1103	62-63	0.45964096687664835
1103	64-65	0.49103051056638236
1103	66-67	0.46691278949394643
1103	68-69	0.40685213649073404
1103	70-71	0.4830329563810736
1103	72-73	0.5147294863124046
1103	74-75	0.45989839183825865
1103	76-77	0.4791659857641619
1103	78-79	0.46848221641455723
1103	80-81	0.5184540424177939
1103	82-83	0.4266896338327868
1103	84-85	0.5441546370523156
1103	86-87	0.5000107778224958
1103	88-89	0.4782212541476767
1103	90-91	0.519319062850947
1103	92-93	0.397825145101649
1103	94-95	0.4434008154829847
1103	96-97	0.4512763013457075
1103	98-99	0.3707842308173639
1103	100	0.420989316349484
1104	1	0.08926873181158612
1104	2	0.2208593761128732
1104	3	0.1824260143282288
1104	4	0.21577181399588596
1104	5	0.1646845505610699
1104	6	0.18620412819251442
1104	7	0.16019155460764978
1104	8	0.15478486873003305
1104	9	0.25770110207515984
1104	10-11	0.22177042498491772
1104	12-13	0.2325014993798149
1104	14-15	0.3232753808053843
1104	16-17	0.27774099499828964
1104	18-19	0.23056927020257234
1104	20-21	0.21772198858553082
1104	22-23	0.20055556984332412
1104	24-25	0.20160998944918873
1104	26-27	0.24047021526765633
1104	28-29	0.2831446655674341
1104	30-31	0.23471506477287818
1104	32-33	0.2538716109460637
1104	34-35	0.2355031393401461
1104	36-37	0.2683274927491084
1104	38-39	0.29128593508326617
1104	40-41	0.32405878452612313
1104	42-43	0.28440455723324476
1104	44-45	0.2152627149726527
1104	46-47	0.2541324160292078
1104	48-49	0.28530629902981275
1104	50-51	0.31916426603520875
1104	52-53	0.263983705698557
1104	54-55	0.2541041587536981
1104	56-57	0.2713165999570819
1104	58-59	0.3167540928189325
1104	60-61	0.3499396485757984
1104	62-63	0.35788330244007227
1104	64-65	0.3761534653935783
1104	66-67	0.3085190446394108
1104	68-69	0.28444980561670263
1104	70-71	0.23262631383432364
1104	72-73	0.14553613553575673
1104	74-75	0.19875971779261903
1104	76-77	0.1983721114779513
1104	78-79	0.12746647353882423
1104	80-81	0.18650025772437573
1104	82-83	0.16175107313186032
1104	84-85	0.1540304651889386
1104	86-87	0.19728169901637216
1104	88-89	0.2351797177275401
1104	90-91	0.28640074411679706
1104	92-93	0.2706155869777902
1104	94-95	0.11478990458265059
1104	96-97	0.12323467686788092
1104	98-99	0.22137687224327962
1104	100	0.005615454922327956
1105	1	0.09323297492261773
1105	2	0.16827788951844624
1105	3	0.21734147207533994
1105	4	0.1857062073505631
1105	5	0.20421109167886442
1105	6	0.20092606564105608
1105	7	0.15314512328511398
1105	8	0.17561060239599868
1105	9	0.26788911745104826
1105	10-11	0.25966683745238583
1105	12-13	0.2681986371990135
1105	14-15	0.3546334836062641
1105	16-17	0.37903136522092495
1105	18-19	0.2936114423162053
1105	20-21	0.3410470317674381
1105	22-23	0.31447155368481106
1105	24-25	0.3106959602816275
1105	26-27	0.3697490549986071
1105	28-29	0.3386202634105615
1105	30-31	0.3545462052962094
1105	32-33	0.3213606247879568
1105	34-35	0.27874363790044043
1105	36-37	0.30967395680745824
1105	38-39	0.3499932470607803
1105	40-41	0.3104543238806343
1105	42-43	0.331477508828776
1105	44-45	0.29006822468017646
1105	46-47	0.41614378307869515
1105	48-49	0.38487641052107335
1105	50-51	0.3505486321021394
1105	52-53	0.379262832993021
1105	54-55	0.3499601943660977
1105	56-57	0.34312765319754845
1105	58-59	0.3991734033013046
1105	60-61	0.4177687820763296
1105	62-63	0.4153512073669319
1105	64-65	0.4586614847204942
1105	66-67	0.41081109049986964
1105	68-69	0.38466865234961034
1105	70-71	0.39152351485116554
1105	72-73	0.37590660925283004
1105	74-75	0.38732816193660824
1105	76-77	0.4154857945270578
1105	78-79	0.3059853248196802
1105	80-81	0.32822566488493976
1105	82-83	0.3636185897295334
1105	84-85	0.3313221379748157
1105	86-87	0.328645907212362
1105	88-89	0.2590180286258672
1105	90-91	0.25645227104590873
1105	92-93	0.37123374194752046
1105	94-95	0.3265799318135443
1105	96-97	0.2522255681631833
1105	98-99	0.23541469158327644
1105	100	0.2467742387797358
1106	1	0.14988075334231965
1106	2	0.09456782131026742
1106	3	0.16779453248638276
1106	4	0.1326142785772788
1106	5	0.1751717275421285
1106	6	0.1788438911302208
1106	7	0.12818391864713874
1106	8	0.18991427883036494
1106	9	0.20523287234944831
1106	10-11	0.16362914269183193
1106	12-13	0.22895771632139628
1106	14-15	0.2645499252262411
1106	16-17	0.2332552732406441
1106	18-19	0.21610253561765802
1106	20-21	0.19946257432028602
1106	22-23	0.18835015499164598
1106	24-25	0.23247989194804575
1106	26-27	0.22963423080373957
1106	28-29	0.23866504882127515
1106	30-31	0.2190331100421261
1106	32-33	0.26012760405679103
1106	34-35	0.22757622219777573
1106	36-37	0.23100785107806132
1106	38-39	0.2177953918341018
1106	40-41	0.24014898254863226
1106	42-43	0.2572773202821992
1106	44-45	0.26130219150314815
1106	46-47	0.27456377618174344
1106	48-49	0.3102489691563761
1106	50-51	0.3352195656988144
1106	52-53	0.29184929682656957
1106	54-55	0.29960568984948566
1106	56-57	0.3011290146740393
1106	58-59	0.3734758425387028
1106	60-61	0.3683100977373428
1106	62-63	0.3344069797585121
1106	64-65	0.2113682869525988
1106	66-67	0.29740035118230423
1106	68-69	0.1470033382023459
1106	70-71	0.24944168742727157
1106	72-73	0.27994591740780805
1106	74-75	0.2122664980242419
1106	76-77	0.25956390364223125
1106	78-79	0.20132120420361588
1106	80-81	0.16175051183080313
1106	82-83	0.11070927346839454
1106	84-85	0.25612832910999117
1106	86-87	0.21345287649404554
1106	88-89	0.2009074747199051
1106	90-91	0.2174044872519616
1106	92-93	0.1682243302934623
1106	94-95	0.13959952655035934
1106	96-97	0.08702209684599893
1106	98-99	0.21058831902239916
1106	100	0.20708893466440514
1107	1	0.05622606268278929
1107	2	0.15394131069254158
1107	3	0.2161089724599634
1107	4	0.14510690846486796
1107	5	0.14445895426925404
1107	6	0.12519137916442702
1107	7	0.06025115858259511
1107	8	0.09596764321008067
1107	9	0.13751471361799616
1107	10-11	0.12432509917242385
1107	12-13	0.10520478019117263
1107	14-15	0.17868066046729325
1107	16-17	0.15817285734162567
1107	18-19	0.10549420347579286
1107	20-21	0.11960010436244062
1107	22-23	0.16896563597233438
1107	24-25	0.1715415557892186
1107	26-27	0.20179011634061794
1107	28-29	0.15863740664448756
1107	30-31	0.23175531978224484
1107	32-33	0.24843578028588809
1107	34-35	0.1642267529139545
1107	36-37	0.22769800678658214
1107	38-39	0.17421416400401313
1107	40-41	0.14516950992867805
1107	42-43	0.14140635341877328
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2216	68-69	-1.416247404777856
2216	70-71	-1.4156146338418623
2216	72-73	-1.452629732579517
2216	74-75	-1.4170392166649393
2216	76-77	-1.3704306122652454
2216	78-79	-1.3634208147768803
2216	80-81	-1.3793499533742946
2216	82-83	-1.3458775950385515
2216	84-85	-1.3284604481981432
2216	86-87	-1.3577066740911867
2216	88-89	-1.4460014749018626
2216	90-91	-1.3735994145976278
2216	92-93	-1.3349717081617811
2216	94-95	-1.3414955388094292
2216	96-97	-1.342665699022195
2216	98-99	-1.4221611209342058
2216	100	-1.4309325555966161
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	86.0
1	38.0
2	34.0
3	42.0
4	20.0
5	53.0
6	143.0
7	611.0
8	2054.0
9	2599.0
10	2278.0
11	2321.0
12	2794.0
13	3444.0
14	5150.0
15	6515.0
16	6681.0
17	6825.0
18	7509.0
19	8025.0
20	9483.0
21	10627.0
22	12578.0
23	15895.0
24	21471.0
25	27137.0
26	29691.0
27	28975.0
28	32058.0
29	39381.0
30	46540.0
31	56082.0
32	71534.0
33	98752.0
34	155283.0
35	253756.0
36	447464.0
37	775860.0
38	593614.0
39	181642.0
40	222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.06961783551256	18.909941027914893	12.521309722902737	21.499131413669808
2	13.673219738068266	23.201036367640697	37.569203880914856	25.556540013376182
3	23.635175808890885	27.34312919993902	30.21743559025459	18.804259400915505
4	12.061761268682766	15.960626853942628	41.36072432558226	30.61688755179235
5	12.409908580544739	40.92239043072206	34.27988417841381	12.387816810319388
6	26.540953007974	38.085713919523386	18.449174774342083	16.924158298160535
7	24.24231182619822	35.35850040351804	22.390175811108364	18.00901195917537
8	22.615648143245423	30.567000765237378	21.469579537640136	25.347771553877063
9	23.921148041348374	20.542452909731708	23.443786782684946	32.09261226623497
10-11	24.23948904955629	24.234480723424888	27.425728799983133	24.100301427035685
12-13	30.701127943484952	22.7315628060092	24.965443672553903	21.601865577951944
14-15	25.952086401107334	24.513096626533493	27.75708423919092	21.77773273316825
16-17	20.857094001181796	27.30952342407244	29.55380197667171	22.279580598074052
18-19	19.72993906141733	32.57638317530538	27.565328497864023	20.12834926541327
20-21	22.48144474501891	28.79277775885369	23.736291606620163	24.989485889507236
22-23	21.759434908783096	27.85811003360667	23.358458241222724	27.02399681638751
24-25	24.609606464970227	27.863422715013648	24.43346017350224	23.093510646513884
26-27	25.748849033613176	24.396929362315216	27.52431017055988	22.32991143351173
28-29	21.67782935205611	28.11296936649751	27.918489406887552	22.290711874558824
30-31	22.09473351203975	29.358279157048944	26.043941812039883	22.503045518871424
32-33	22.417482162521313	29.218742885666444	24.80897750900938	23.554797442802865
34-35	24.030243521564152	26.433991330325394	25.52614604211057	24.00961910599988
36-37	25.04204398883954	25.421283129246564	28.00139775603491	21.535275125878993
38-39	26.11325107766617	24.576370946524822	28.062477654026996	21.247900321782012
40-41	22.271210891866062	26.550095632186853	29.401960270100847	21.776733205846238
42-43	21.36900743225386	29.004005122481995	25.660663058846307	23.966324386417835
44-45	22.22004645680819	28.731926810624753	24.720911703054234	24.327115029512825
46-47	23.10506457429206	27.69204041944195	23.94573634258361	25.25715866368238
48-49	24.336236988160206	26.419244577468888	25.588308467151933	23.656209967218977
50-51	23.95271938749349	27.452819240951793	27.096619015876698	21.497842355678017
52-53	21.946291122256305	28.504431323966088	26.805643286221613	22.743634267555997
54-55	20.221583565801197	26.713146745050704	28.07389762327825	24.99137206586985
56-57	24.07532014977124	27.50460043712798	24.65807311140016	23.762006301700623
58-59	23.52477012471426	26.360832004145447	26.050328316155095	24.064069554985196
60-61	22.784982009428205	25.66379398901859	28.216901640801545	23.334322360751656
62-63	25.709450108380654	25.157097554060027	25.93064751264244	23.20280482491687
64-65	22.883582600112238	27.801790699569086	27.252598076316165	22.062028624002515
66-67	22.746561977515718	25.897171719625234	27.12643934088207	24.229826961976975
68-69	21.094372022725125	28.71636438470777	26.675927425340408	23.513336167226697
70-71	23.146907385178633	27.902862428925086	24.012710660747892	24.937519525148392
72-73	22.864235447593085	26.023927384249802	25.691247446032495	25.420589722124625
74-75	22.874135359221796	29.075596175583375	25.369138588630964	22.68112987656387
76-77	24.504764820535918	25.245966623062593	26.052009456264773	24.197259100136716
78-79	23.025617211532897	25.99582990140505	27.835602102895606	23.142950784166445
80-81	23.179454878105147	28.611578467587318	26.52078846964192	21.688178184665617
82-83	22.7226470272206	26.577486011685224	27.26376569768844	23.43610126340573
84-85	23.232750201482247	26.648064066800686	25.776857861990816	24.34232786972625
86-87	23.78647106022141	27.16969274061045	26.655578118350604	22.388258080817536
88-89	25.16136965132281	24.730488351817463	26.238606633844846	23.86953536301488
90-91	23.296118022086972	24.87391318061624	28.117736018620082	23.712232778676707
92-93	23.533186124694634	28.05458065863356	26.764736789638338	21.64749642703347
94-95	22.121243404102284	28.480108820293687	25.944713380179767	23.45393439542426
96-97	21.08594004722505	27.041258920901328	25.906258660301624	25.966542371572
98-99	23.15221573201695	27.888431364181702	24.480992072039314	24.47836083176204
100	22.426677946982515	27.670371899599793	25.317022752235147	24.585927401182545
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3716.0
1	2318.5
2	963.0
3	938.0
4	852.0
5	655.5
6	441.0
7	444.0
8	527.0
9	576.5
10	453.5
11	297.0
12	269.0
13	284.5
14	321.0
15	376.5
16	430.0
17	475.0
18	616.0
19	790.5
20	927.5
21	1136.0
22	1460.5
23	1919.5
24	2637.5
25	3557.0
26	4956.5
27	6530.5
28	8857.5
29	13404.5
30	19010.5
31	24775.0
32	31893.0
33	38574.0
34	43371.0
35	46914.5
36	50368.0
37	58052.0
38	61139.5
39	59904.0
40	57187.0
41	51948.0
42	49798.0
43	53692.5
44	64450.0
45	81264.5
46	105628.5
47	174093.0
48	422304.0
49	432084.0
50	207396.0
51	142352.0
52	105897.0
53	89672.5
54	80408.5
55	68300.5
56	60524.5
57	49415.5
58	37713.0
59	35010.0
60	29150.0
61	21591.5
62	16410.0
63	13144.5
64	9484.0
65	4677.0
66	2346.5
67	1593.5
68	1147.5
69	699.5
70	470.5
71	383.5
72	342.5
73	290.5
74	206.5
75	174.5
76	183.5
77	150.5
78	89.5
79	46.0
80	28.0
81	29.0
82	30.0
83	21.5
84	19.5
85	16.5
86	15.0
87	13.0
88	10.5
89	11.5
90	10.5
91	6.5
92	5.0
93	4.5
94	6.0
95	5.0
96	3.5
97	6.0
98	6.5
99	9.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.061748233801543005
2	0.009982237687196464
3	0.011567255157798606
4	0.010184580343018014
5	0.012208006901233515
6	0.012848758644668423
7	0.003237482493144799
8	0.0058679370188249496
9	0.007318059385546058
10-11	0.0068965121859178285
12-13	0.006087141562631628
14-15	0.01028575167092879
16-17	0.009543828599583106
18-19	0.00784077791308506
20-21	0.0068796502979326995
22-23	0.002765349629561183
24-25	0.003979405564490483
26-27	0.00505856639553875
28-29	0.006070279674646499
30-31	0.008599562872415874
32-33	0.010184580343018014
34-35	0.011280603062051411
36-37	0.01736774462468304
38-39	0.017772429936326137
40-41	0.017620672944459976
42-43	0.023218819755522858
44-45	0.026742954344414854
46-47	0.028732657126660094
48-49	0.030334536485247365
50-51	0.031127045220548436
52-53	0.028985585446437034
54-55	0.03567975497653331
56-57	0.03866430914990117
58-59	0.0354436885447415
60-61	0.03626992105601283
62-63	0.03191955395584951
64-65	0.03394298051406501
66-67	0.035848373856384605
68-69	0.038681171037886306
70-71	0.038478828382064754
72-73	0.03394298051406501
74-75	0.037180463007209806
76-77	0.036910672799447736
78-79	0.036033854624221026
80-81	0.04033363606042896
82-83	0.036978120351388255
84-85	0.03325164310667471
86-87	0.03195327773181976
88-89	0.02959261341390168
90-91	0.031717211300027956
92-93	0.03350457142645165
94-95	0.03756828643086778
96-97	0.032054449059730546
98-99	0.029862403621663747
100	0.027349982311879503
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2965267.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.49729588769527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.28632005720942	35.49269100786155
2	14.14141456845325	14.282063914674966
3	6.002643151296358	9.09351741957779
4	3.2098689344068023	6.4835880536584565
5	1.8507129228728207	4.672799903474509
6	1.2160342158809965	3.684386376534251
7	0.7992803659013945	2.825304799390364
8	0.5511568384499334	2.226554396138636
9	0.38543022685675044	1.7516865788682154
>10	1.5282524368241346	11.950911821038664
>50	0.022961732239022443	0.7568868370962175
>100	0.004557495020131911	0.3758609834986471
>500	1.1392121574875325E-4	0.05073893165483773
>1k	6.835272944925194E-4	0.8992066221051797
>5k	1.1392121574875325E-4	0.2886136282452617
>10k+	4.55684862995013E-4	5.165188726182443
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	55391	1.867993674768579	No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG	13473	0.4543604336472905	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	10887	0.3671507489882024	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	10036	0.3384518156375126	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	5017	0.16919218404278602	No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC	4608	0.15539915967095036	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4311	0.14538319820778364	No Hit
ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT	3019	0.1018120796542099	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	5.0585663955387494E-5	0.0
12-13	0.0	0.0	0.0	1.348951038810333E-4	0.0
14-15	0.0	0.0	0.0	1.348951038810333E-4	0.0
16-17	0.0	0.0	0.0	1.5175699186616246E-4	0.0
18-19	0.0	0.0	0.0	3.035139837323249E-4	0.0
20-21	0.0	0.0	0.0	5.901660794795207E-4	0.0
22-23	0.0	0.0	0.0	0.001534431806646754	0.0
24-25	0.0	0.0	0.0	0.0036421678047878995	0.0
26-27	0.0	0.0	0.0	0.006744755194051665	0.0
28-29	0.0	0.0	0.0	0.015867036594006543	0.0
30-31	0.0	0.0	0.0	0.04031677417244383	0.0
32-33	0.0	0.0	0.0	0.09761346954591273	0.0
34-35	0.0	0.0	0.0	0.20139839009438273	0.0
36-37	0.0	0.0	0.0	0.3381820254297505	0.0
38-39	0.0	0.0	0.0	0.4831774002138762	0.0
40-41	0.0	0.0	0.0	0.6356088675994438	0.0
42-43	0.0	0.0	0.0	0.7853592947953759	0.0
44-45	0.0	0.0	0.0	0.9559341536529425	0.0
46-47	0.0	0.0	0.0	1.1769091956980602	0.0
48-49	0.0	0.0	0.0	1.4051011258008133	0.0
50-51	0.0	0.0	0.0	1.6101754074759542	0.0
52-53	0.0	0.0	0.0	1.7992477574532075	0.0
54-55	0.0	0.0	0.0	2.0060419517028314	0.0
56-57	0.0	0.0	0.0	2.2478245635215988	0.0
58-59	0.0	0.0	0.0	2.5425197798377006	0.0
60-61	0.0	0.0	0.0	2.8946803104071233	0.0
62-63	0.0	0.0	0.0	3.184789093191271	0.0
64-65	0.0	0.0	0.0	3.4467216611522673	0.0
66-67	0.0	0.0	0.0	3.7440641938820347	0.0
68-69	0.0	0.0	0.0	4.0618433348497796	0.0
70-71	0.0	0.0	0.0	4.4092825367833655	0.0
72-73	0.0	0.0	0.0	4.768457612754602	0.0
74-75	0.0	0.0	0.0	5.119758187036783	0.0
76-77	0.0	0.0	0.0	5.46197020369498	0.0
78-79	0.0	0.0	0.0	5.865508906955091	0.0
80-81	0.0	0.0	0.0	6.278473405598889	0.0
82-83	0.0	0.0	0.0	6.6832430266819145	0.0
84-85	0.0	1.6861887985129163E-5	0.0	7.210497402088919	0.0
86-87	0.0	3.372377597025833E-5	0.0	7.755001488904709	1.6861887985129163E-5
88	0.0	3.372377597025833E-5	0.0	8.108814484496675	3.372377597025833E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCGC	230	0.0	34.734	9
GGGCACC	1495	0.0	32.069725	94
TATTCTC	1025	0.0	29.342503	5
AGGGCTA	1985	0.0	28.40938	5
CGTTAAC	675	0.0	27.164373	1
GTACTAG	1945	0.0	26.831333	1
TAACCAC	650	0.0	26.74854	6
GCTATTG	2110	0.0	26.503193	8
CTATTGA	2150	0.0	25.791538	9
CATGGGG	6490	0.0	25.271387	4
ATGGGGG	5315	0.0	24.403204	5
TACCCCG	135	1.4599302E-5	24.367182	5
GGGCTAT	2455	0.0	24.308826	6
GGGTAAA	280	3.6379788E-12	23.50763	1
TAGGCAT	2395	0.0	23.349764	5
GTGATCG	1050	0.0	23.272753	8
CTTTGCG	930	0.0	23.244272	2
TCGCAAT	225	3.1723175E-9	22.985239	1
TGGGGGG	5220	0.0	22.955217	6
GCATCGT	2015	0.0	22.855186	8
>>END_MODULE