##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146539_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2965267
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53161587135324	34.0	31.0	34.0	31.0	34.0
2	32.57080323626844	34.0	31.0	34.0	31.0	34.0
3	32.730797260415336	34.0	31.0	34.0	31.0	34.0
4	36.10937969498194	37.0	35.0	37.0	35.0	37.0
5	36.16756737251654	37.0	35.0	37.0	35.0	37.0
6	36.2902497481677	37.0	37.0	37.0	35.0	37.0
7	36.29583305651734	37.0	37.0	37.0	35.0	37.0
8	36.31775317366025	37.0	37.0	37.0	35.0	37.0
9	38.12844172211136	39.0	39.0	39.0	37.0	39.0
10-11	38.10053934434909	39.0	38.0	39.0	37.0	39.0
12-13	38.08243625278938	39.0	38.0	39.0	37.0	39.0
14-15	39.399474313780175	41.0	39.0	41.0	36.0	41.0
16-17	39.42204057847067	41.0	39.0	41.0	36.5	41.0
18-19	39.54973346413662	41.0	39.5	41.0	37.0	41.0
20-21	39.57648602975719	41.0	40.0	41.0	37.0	41.0
22-23	39.54188391804178	41.0	39.5	41.0	37.0	41.0
24-25	39.49126571064258	41.0	39.0	41.0	36.0	41.0
26-27	39.29266605671597	41.0	39.0	41.0	35.5	41.0
28-29	39.188455710733635	40.5	39.0	41.0	35.5	41.0
30-31	39.179714170764385	40.5	39.0	41.0	35.5	41.0
32-33	39.063381307652904	40.5	39.0	41.0	35.0	41.0
34-35	38.98373232494747	40.0	38.5	41.0	35.0	41.0
36-37	38.84828381390276	40.0	38.0	41.0	35.0	41.0
38-39	38.66184444773438	40.0	38.0	41.0	35.0	41.0
40-41	38.57583414916768	40.0	38.0	41.0	35.0	41.0
42-43	38.55369583919425	40.0	38.0	41.0	35.0	41.0
44-45	38.416934967407656	40.0	38.0	41.0	35.0	41.0
46-47	38.15304692629702	40.0	38.0	41.0	34.0	41.0
48-49	37.869476509197995	40.0	37.0	41.0	33.0	41.0
50-51	37.62889648723032	40.0	37.0	41.0	33.0	41.0
52-53	37.3753186475282	40.0	36.5	41.0	32.5	41.0
54-55	37.258263589754314	40.0	36.0	41.0	32.5	41.0
56-57	37.08518659533863	39.0	35.5	41.0	32.5	41.0
58-59	36.85661139452198	39.0	35.0	41.0	32.0	41.0
60-61	36.52927527268203	39.0	35.0	41.0	31.0	41.0
62-63	36.2075969887366	38.0	35.0	40.0	31.0	41.0
64-65	35.793995953821366	37.0	35.0	40.0	30.0	41.0
66-67	35.49051518800836	37.0	35.0	39.0	30.0	41.0
68-69	35.15654155258194	36.0	35.0	39.0	30.0	41.0
70-71	34.77427192896964	35.5	35.0	38.0	30.0	40.5
72-73	34.35519786245219	35.0	34.0	37.0	29.5	39.5
74-75	34.067351439179006	35.0	34.0	37.0	29.0	39.0
76-77	33.693099643303626	35.0	34.0	36.0	29.0	39.0
78-79	33.42651690387409	35.0	34.0	36.0	29.0	38.0
80-81	33.323631227811866	35.0	34.0	36.0	29.0	37.0
82-83	33.12283227783536	35.0	34.0	35.0	29.0	37.0
84-85	32.98812805052631	35.0	34.0	35.0	29.0	36.0
86-87	32.86028070996642	35.0	34.0	35.0	28.5	36.0
88-89	32.65120425917801	35.0	33.5	35.0	27.0	36.0
90-91	32.5266434692053	35.0	33.0	35.0	27.0	36.0
92-93	32.55729838156227	35.0	33.0	35.0	27.0	35.5
94-95	32.55746345944564	35.0	33.5	35.0	27.0	35.0
96-97	32.47589542526862	35.0	33.0	35.0	27.0	35.0
98-99	32.43406900626486	35.0	33.0	35.0	27.0	35.0
100	31.642071692026384	35.0	32.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.29103585439187185
1101	2	0.22142024830644402
1101	3	0.26668217848042275
1101	4	0.1532980964549111
1101	5	0.18866795586704654
1101	6	0.162926241185545
1101	7	0.1834629324174344
1101	8	0.15800272182999464
1101	9	0.20600753472426447
1101	10-11	0.21605958418506788
1101	12-13	0.23064456139631773
1101	14-15	0.30396357541026475
1101	16-17	0.316316705568795
1101	18-19	0.299103395321481
1101	20-21	0.30943192153440435
1101	22-23	0.3348141880872504
1101	24-25	0.35616762848283656
1101	26-27	0.3753415141223968
1101	28-29	0.3675765261590982
1101	30-31	0.38170151322540846
1101	32-33	0.38100403883480993
1101	34-35	0.45273688400845913
1101	36-37	0.47451746408648887
1101	38-39	0.4599907486541355
1101	40-41	0.43667650999186947
1101	42-43	0.46113206589748756
1101	44-45	0.4895635496735764
1101	46-47	0.5084080916044655
1101	48-49	0.5024989166067897
1101	50-51	0.4127821357412529
1101	52-53	0.5223361550608843
1101	54-55	0.5183259633694419
1101	56-57	0.49913438161878076
1101	58-59	0.4896704547838624
1101	60-61	0.36953447720942734
1101	62-63	0.4453619592240017
1101	64-65	0.46350086495829146
1101	66-67	0.45981909928621434
1101	68-69	0.4303591993327416
1101	70-71	0.40800733701631486
1101	72-73	0.30124056813421873
1101	74-75	0.3629805025085773
1101	76-77	0.3699005046629935
1101	78-79	0.40638553516028253
1101	80-81	0.36748985670983814
1101	82-83	0.39279256513166416
1101	84-85	0.38740347509186535
1101	86-87	0.4619966725349727
1101	88-89	0.40473489819557074
1101	90-91	0.4325210597165281
1101	92-93	0.43427524245299054
1101	94-95	0.4452306667254362
1101	96-97	0.398349316841319
1101	98-99	0.4193087369786852
1101	100	0.4419273911422579
1102	1	0.26703639187187633
1102	2	0.2815160777614949
1102	3	0.24230238542175897
1102	4	0.180903346227403
1102	5	0.15853245554352213
1102	6	0.16408366861709567
1102	7	0.13956791518000955
1102	8	0.10540378687084484
1102	9	0.13316962300708468
1102	10-11	0.20034153800560972
1102	12-13	0.19415092349866825
1102	14-15	0.261977419557212
1102	16-17	0.2696443238518782
1102	18-19	0.24879826584761844
1102	20-21	0.2158998763076525
1102	22-23	0.25254255076860943
1102	24-25	0.21433980431953614
1102	26-27	0.2550265865956902
1102	28-29	0.27625978377234617
1102	30-31	0.2853897246511252
1102	32-33	0.2679670778574774
1102	34-35	0.2916273251688182
1102	36-37	0.28644934714652237
1102	38-39	0.2960765043960052
1102	40-41	0.3194586523917735
1102	42-43	0.2395230629920917
1102	44-45	0.2283149640187503
1102	46-47	0.24203657758326358
1102	48-49	0.2796358898100735
1102	50-51	0.3030217410971403
1102	52-53	0.30882698156902677
1102	54-55	0.2701209274355634
1102	56-57	0.25753560640421824
1102	58-59	0.25542930215452486
1102	60-61	0.3007730081820057
1102	62-63	0.30104519030926724
1102	64-65	0.2957094983173505
1102	66-67	0.28062603439300204
1102	68-69	0.24591092659278502
1102	70-71	0.246672362408205
1102	72-73	0.20492294052019844
1102	74-75	0.21390433951603427
1102	76-77	0.27103164920705325
1102	78-79	0.2951359691234856
1102	80-81	0.20654789993660927
1102	82-83	0.2725273775730628
1102	84-85	0.28842778683488035
1102	86-87	0.23117229527937155
1102	88-89	0.234350781652374
1102	90-91	0.2812556172586085
1102	92-93	0.21312045916931055
1102	94-95	0.2976290414827929
1102	96-97	0.3062957020576178
1102	98-99	0.2830533030673905
1102	100	0.3518112562463074
1103	1	0.23444803405169523
1103	2	0.21876240027422966
1103	3	0.23888526603834492
1103	4	0.15099114651464873
1103	5	0.11983409424433944
1103	6	0.12643068157861848
1103	7	0.11673359612844081
1103	8	0.17119657640885322
1103	9	0.205891431911553
1103	10-11	0.22036143078087633
1103	12-13	0.2250427085853346
1103	14-15	0.29665039303648655
1103	16-17	0.2544097362924447
1103	18-19	0.2727685582624986
1103	20-21	0.3043574948837673
1103	22-23	0.2981518116137494
1103	24-25	0.33000586662049614
1103	26-27	0.2948168761559984
1103	28-29	0.3294110714504228
1103	30-31	0.39022320071289585
1103	32-33	0.36411474826184786
1103	34-35	0.3077361243766745
1103	36-37	0.3216937918883218
1103	38-39	0.39011865165605997
1103	40-41	0.37683241578371707
1103	42-43	0.31478554949980264
1103	44-45	0.2524695210699832
1103	46-47	0.28134961894775046
1103	48-49	0.2826705415973052
1103	50-51	0.286990996500748
1103	52-53	0.3196888382875187
1103	54-55	0.34618347371079494
1103	56-57	0.3159310489069611
1103	58-59	0.29519673286434056
1103	60-61	0.17763098670137367
1103	62-63	0.24186502019480827
1103	64-65	0.2383362924583423
1103	66-67	0.2083678141273495
1103	68-69	0.20437500140032228
1103	70-71	0.17402693551638748
1103	72-73	0.2269992935411409
1103	74-75	0.18447998112494446
1103	76-77	0.2115651859284
1103	78-79	0.22166790238009781
1103	80-81	0.24318366812779857
1103	82-83	0.24194976229754417
1103	84-85	0.1766229189032842
1103	86-87	0.21204501273951593
1103	88-89	0.23793766981572873
1103	90-91	0.2084052970116872
1103	92-93	0.22673067451083284
1103	94-95	0.15230780215397743
1103	96-97	0.2180820041423459
1103	98-99	0.25599567200254114
1103	100	0.23912771640277697
1104	1	0.204437117526183
1104	2	0.17329637266985287
1104	3	0.17332913832682095
1104	4	0.11450049797893058
1104	5	0.06195824034755759
1104	6	0.0790584677503503
1104	7	0.10500549410558335
1104	8	0.1202104485185842
1104	9	0.10698259817394273
1104	10-11	0.1451501054573825
1104	12-13	0.12450033890283407
1104	14-15	0.2470229165123996
1104	16-17	0.22263372299809703
1104	18-19	0.1684199604564256
1104	20-21	0.1832486592535716
1104	22-23	0.19759994615749576
1104	24-25	0.20481473542346862
1104	26-27	0.19718688328472211
1104	28-29	0.23425185262468062
1104	30-31	0.21046248632579534
1104	32-33	0.19688746951271696
1104	34-35	0.24138018308460119
1104	36-37	0.2624694066887372
1104	38-39	0.21884909759074134
1104	40-41	0.2434724877123955
1104	42-43	0.29158931279650346
1104	44-45	0.31422464497635616
1104	46-47	0.34294883473484106
1104	48-49	0.2803272212071306
1104	50-51	0.2888308108803912
1104	52-53	0.35882351766814224
1104	54-55	0.3489049164690883
1104	56-57	0.28601285581930114
1104	58-59	0.3122684583535431
1104	60-61	0.3033710984794453
1104	62-63	0.2700781468895954
1104	64-65	0.36826880477853763
1104	66-67	0.3327931719570216
1104	68-69	0.3432673841206153
1104	70-71	0.2920260859567776
1104	72-73	0.2959987728178817
1104	74-75	0.21371211277303814
1104	76-77	0.22844275734450292
1104	78-79	0.23237686802024626
1104	80-81	0.20729013618887393
1104	82-83	0.253024430228038
1104	84-85	0.206809622244009
1104	86-87	0.24724443318846312
1104	88-89	0.27913457496891425
1104	90-91	0.2868191952495849
1104	92-93	0.2351948260920338
1104	94-95	0.297743857284928
1104	96-97	0.3171992520423643
1104	98-99	0.3023194078357676
1104	100	0.1643025306404695
1105	1	0.13978406267308685
1105	2	0.1398851160978225
1105	3	0.18770186612497497
1105	4	0.12680775462297333
1105	5	0.08499589174477507
1105	6	0.06347845876813807
1105	7	0.1219466923406003
1105	8	0.1010719594445817
1105	9	0.12802187262323628
1105	10-11	0.14236101766380926
1105	12-13	0.1596945070483784
1105	14-15	0.18306475747793627
1105	16-17	0.22287947847530631
1105	18-19	0.16965223171551003
1105	20-21	0.18140803955656537
1105	22-23	0.18822857919327873
1105	24-25	0.12809991292169798
1105	26-27	0.19368942753492746
1105	28-29	0.23672394546174758
1105	30-31	0.2138623208938668
1105	32-33	0.20721950352006502
1105	34-35	0.236846438666511
1105	36-37	0.2743979038069426
1105	38-39	0.2678480319002361
1105	40-41	0.3164285838220948
1105	42-43	0.2957144813314727
1105	44-45	0.2570664511689671
1105	46-47	0.27244955316988495
1105	48-49	0.32749524760233584
1105	50-51	0.3397862548794066
1105	52-53	0.3259307163257432
1105	54-55	0.26340664516641255
1105	56-57	0.3760200295105136
1105	58-59	0.36885590727193573
1105	60-61	0.32083220278967417
1105	62-63	0.31463980320808105
1105	64-65	0.341304217728279
1105	66-67	0.2889035828459683
1105	68-69	0.26498789867455486
1105	70-71	0.23443678374600552
1105	72-73	0.2483680747254624
1105	74-75	0.22267576251783083
1105	76-77	0.23352406273127002
1105	78-79	0.2339862110868367
1105	80-81	0.2011795890981034
1105	82-83	0.2575875468670219
1105	84-85	0.2535905393489699
1105	86-87	0.23446964895585154
1105	88-89	0.3014559231728029
1105	90-91	0.2752751129941018
1105	92-93	0.24171519264788088
1105	94-95	0.21395950836758004
1105	96-97	0.21709984839319674
1105	98-99	0.18171431521378167
1105	100	0.2556403666089331
1106	1	0.14197457309241202
1106	2	0.1182223009764698
1106	3	0.155243863643868
1106	4	0.1001355233690262
1106	5	0.027994476525798007
1106	6	0.045395845360282294
1106	7	0.06848584374663602
1106	8	0.06029674637798621
1106	9	0.07474402841376815
1106	10-11	0.13239438839296014
1106	12-13	0.09747248449444612
1106	14-15	0.1577147874841529
1106	16-17	0.151747818324921
1106	18-19	0.16691533928558755
1106	20-21	0.1786744500506714
1106	22-23	0.22276034212618612
1106	24-25	0.20735309940366164
1106	26-27	0.22252902074904313
1106	28-29	0.18635706897542548
1106	30-31	0.22405749571371558
1106	32-33	0.20622963397996585
1106	34-35	0.16943902130678623
1106	36-37	0.2183762254288979
1106	38-39	0.2235707956527193
1106	40-41	0.21506419628497753
1106	42-43	0.19851175009319633
1106	44-45	0.21606146095457746
1106	46-47	0.2305259582001824
1106	48-49	0.15854964732108812
1106	50-51	0.23533808391557898
1106	52-53	0.2561986562416365
1106	54-55	0.203950429623184
1106	56-57	0.2171137508198555
1106	58-59	0.15348356696551235
1106	60-61	0.1666727297796058
1106	62-63	0.2589496208418893
1106	64-65	0.27838648180993175
1106	66-67	0.1810394046806465
1106	68-69	0.2100025267093244
1106	70-71	0.12013106397726858
1106	72-73	0.12216197562430153
1106	74-75	0.1172001582506752
1106	76-77	0.1444551458216452
1106	78-79	0.07158152003899687
1106	80-81	0.05312993775252295
1106	82-83	0.10625540482561036
1106	84-85	0.1075110821929357
1106	86-87	0.14874790966964468
1106	88-89	0.14263353315457294
1106	90-91	0.1507780251654296
1106	92-93	0.14769586438425364
1106	94-95	0.13173380221361697
1106	96-97	0.19052939198508057
1106	98-99	0.12577210294885788
1106	100	0.07665082624160391
1107	1	0.13324727065567998
1107	2	0.1463018505315432
1107	3	0.12666294219258134
1107	4	0.060642069662861786
1107	5	0.06475775464286215
1107	6	0.06890885284273196
1107	7	0.052719181845859
1107	8	0.06896059435963053
1107	9	0.09931860441953688
1107	10-11	0.10351348804777416
1107	12-13	0.09727612972140776
1107	14-15	0.1952038429523384
1107	16-17	0.17099317328178643
1107	18-19	0.1523498184756633
1107	20-21	0.13007119698858816
1107	22-23	0.16388999482879285
1107	24-25	0.1618307916223216
1107	26-27	0.1359273063894051
1107	28-29	0.20975338017459677
1107	30-31	0.22086489237895535
1107	32-33	0.17139926571264397
1107	34-35	0.22599872772781282
1107	36-37	0.23847222412167923
1107	38-39	0.27810921895929397
1107	40-41	0.2177149857210381
1107	42-43	0.2059647890305456
1107	44-45	0.20829754965465241
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2216	54-55	-0.8612627920545037
2216	56-57	-0.8998775083309951
2216	58-59	-0.9206940999803734
2216	60-61	-0.9387767812656094
2216	62-63	-0.9565032641037021
2216	64-65	-0.9699496156344125
2216	66-67	-0.8826620608999534
2216	68-69	-0.8127749715816606
2216	70-71	-0.8579854611540725
2216	72-73	-0.8689477166567272
2216	74-75	-0.8668416741021758
2216	76-77	-0.8671107732111594
2216	78-79	-0.9710812094744838
2216	80-81	-0.9469160973328314
2216	82-83	-0.8648249333186371
2216	84-85	-0.9046272966659856
2216	86-87	-1.0130376940057886
2216	88-89	-0.9817619128421562
2216	90-91	-0.9507498582556764
2216	92-93	-1.0186245261697877
2216	94-95	-1.011664967135161
2216	96-97	-1.0034208155037803
2216	98-99	-1.052992704502131
2216	100	-1.3692806350383755
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	11.0
8	53.0
9	210.0
10	489.0
11	524.0
12	347.0
13	315.0
14	374.0
15	616.0
16	998.0
17	2128.0
18	3875.0
19	6023.0
20	8559.0
21	13129.0
22	19634.0
23	23298.0
24	22772.0
25	19550.0
26	17190.0
27	18749.0
28	23365.0
29	27068.0
30	33216.0
31	42070.0
32	54475.0
33	73277.0
34	109895.0
35	186037.0
36	362961.0
37	880604.0
38	798870.0
39	214203.0
40	382.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.369844635459756	18.6195971725657	12.768950430189157	21.24160776178539
2	13.815240275541923	23.09839177195385	37.68724769641915	25.399120256085077
3	23.450579090553152	27.38481337005645	30.949871731012497	18.214735808377903
4	12.06370286385678	16.101214494344017	41.03755243625616	30.797530205543044
5	12.072781350959632	40.46887103347575	34.61176172110109	12.846585894463534
6	26.327644860121975	38.13927872709325	18.13073944081165	17.40233697197313
7	23.556597095640967	35.74821424175293	22.447894236842753	18.247294425763346
8	23.07245856781194	29.546951421237956	21.528314313685748	25.85227569726436
9	24.137590308056573	19.402536095400517	23.778600712853176	32.68127288368973
10-11	24.368362107021056	23.6231172437423	27.652653201212573	24.355867448024075
12-13	31.28751980850291	22.18176305877346	25.49004861956782	21.040668513155815
14-15	26.141516477779735	23.673559134323867	29.02581083788099	21.159113550015412
16-17	20.35172549385941	26.61079086638741	30.70425024120931	22.333233398543875
18-19	19.207224846868765	30.832147661576514	30.05280806079183	19.907819430762896
20-21	21.742679495640697	27.126241920204823	26.687529318607734	24.443549265546746
22-23	20.946698468342912	26.35461904171736	26.299244330794764	26.39943815914496
24-25	23.881070406138807	26.388534320855424	27.356575310081688	22.37381996292408
26-27	24.9836692614864	22.98475988840128	30.355040541037283	21.676530309075034
28-29	20.939912463150435	26.592880131644197	30.91117810529951	21.55602929990586
30-31	21.53284548453206	27.759173469604555	28.950366291286812	21.757614754576576
32-33	21.976703952214066	27.51018135560754	27.710031350076758	22.803083342101644
34-35	23.342505196902227	24.940527338875796	28.337686005932767	23.37928145828921
36-37	24.414799521590886	24.057982402462933	30.567664824941083	20.959553251005104
38-39	25.486081087370156	23.09877494143825	30.75210286094307	20.663041110248525
40-41	21.705846079549065	25.106109094870156	31.85965726395606	21.32838756162472
42-43	21.103155597238537	27.51494904795195	28.075679565795543	23.30621578901397
44-45	21.938211923891032	27.321976556096395	26.95167032375195	23.788141196260618
46-47	23.052641855867684	26.32957811884275	25.74559789050147	24.872182134788094
48-49	24.227364916590282	25.46979302195234	27.007812515808155	23.295029545649225
50-51	23.71580551212649	26.61501043421722	28.26322218340211	21.405961870254185
52-53	21.7532806965005	27.61364740066855	28.099563473113665	22.533508429717283
54-55	20.07662495421928	25.875533096678765	29.152016273992302	24.895825675109652
56-57	23.960306720793866	26.809623175209406	25.577344141911063	23.65272596208567
58-59	23.221425402516296	25.672971095786256	27.09279555563125	24.012807946066197
60-61	22.484501082049245	25.162071315763114	29.14250168706603	23.210925915121607
62-63	25.209663189929156	24.656536149616624	26.915313877632506	23.218486782821714
64-65	22.495907868140524	27.228768003145888	28.177963850966304	22.097360277747285
66-67	22.350070569904677	25.37132248169988	28.131086338104883	24.14752061029056
68-69	20.765123060937416	27.976984018046934	27.740841378719576	23.517051542296073
70-71	22.614012658086974	27.222441242957096	25.070121296268503	25.093424802687426
72-73	22.390739115984147	25.111795775983843	26.795018003508464	25.70244710452355
74-75	22.491760132768686	28.250257912144285	26.19260969992483	23.065372255162206
76-77	24.121057456037697	24.4176693689434	26.71215390499111	24.749119270027798
78-79	22.480792043146554	25.223923507099332	28.63755662479544	23.657727824958666
80-81	22.590649164686656	27.838578986798645	27.43635517168873	22.134416676825964
82-83	22.137940686482803	25.94263234927123	28.093691615575022	23.825735348670943
84-85	22.631719344961983	25.955050192849672	26.53257063538715	24.880659826801196
86-87	23.160987385113614	26.53694027164169	27.297580166909114	23.004492176335585
88-89	24.362522065943974	24.12898432666686	27.080193150043026	24.428300457346143
90-91	22.469173939479987	24.22173112910237	28.944105201993615	24.36498972942403
92-93	22.631402163784912	27.415473884813746	27.559356374990852	22.39376757641049
94-95	21.229532397689177	27.814153572420413	26.840969215066913	24.115344814823494
96-97	20.1397011895257	26.49528750875767	26.659623995555194	26.705387306161438
98-99	22.276600157490673	27.076474030058122	25.3673967331646	25.279529079286604
100	21.52655176890948	26.842773531360812	26.270982667536746	25.359692032192953
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	972.0
1	594.5
2	461.5
3	658.5
4	388.5
5	236.0
6	517.0
7	1180.0
8	2169.0
9	2616.5
10	1794.0
11	981.5
12	901.5
13	1052.5
14	1345.5
15	1724.5
16	2232.0
17	2780.5
18	3375.0
19	3980.5
20	4484.0
21	4936.5
22	5473.0
23	6027.5
24	6714.0
25	7427.5
26	8676.0
27	10150.5
28	12005.5
29	16013.5
30	20839.0
31	25428.0
32	31750.0
33	37597.0
34	41647.0
35	44453.0
36	47442.5
37	55410.5
38	58805.5
39	58134.0
40	55728.0
41	50503.5
42	48721.5
43	51984.5
44	61786.0
45	78253.5
46	99768.0
47	168499.5
48	426797.5
49	434333.5
50	208345.0
51	145141.0
52	100177.5
53	82907.0
54	76746.0
55	66554.0
56	58749.0
57	48174.0
58	37433.0
59	35446.5
60	29044.0
61	20599.5
62	15385.5
63	12379.0
64	8797.5
65	4040.0
66	1900.0
67	1254.5
68	901.0
69	514.0
70	313.5
71	247.0
72	239.0
73	208.5
74	122.0
75	86.5
76	114.0
77	104.0
78	51.5
79	16.0
80	4.5
81	2.0
82	1.5
83	1.5
84	1.5
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005935384570765465
2	0.09489870558030693
3	4.721328635836166E-4
4	0.0
5	0.040839492699982834
6	0.004991118843598232
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.372377597025833E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.889947515687457E-4
24-25	0.0
26-27	0.0
28-29	5.058566395538749E-5
30-31	1.348951038810333E-4
32-33	0.001450122366721108
34-35	0.0013152272628400748
36-37	7.587849593308124E-4
38-39	7.081992953754248E-4
40-41	0.0013658129267954623
42-43	9.442657271672332E-4
44-45	6.23889855449779E-4
46-47	4.215471996282291E-4
48-49	8.430943992564582E-4
50-51	7.756468473159414E-4
52-53	0.001450122366721108
54-55	0.0015175699186616247
56-57	9.948513911226208E-4
58-59	0.002579868861724762
60-61	0.0020234265582154995
62-63	0.0011803321589590415
64-65	0.00423233388426742
66-67	0.0041143006683715165
68-69	0.0037096153567284158
70-71	0.0016187412465723996
72-73	0.005075428283523878
74-75	0.0037096153567284158
76-77	0.003810786684639191
78-79	8.262325112713291E-4
80-81	8.430943992564582E-5
82-83	2.360664317918083E-4
84-85	1.348951038810333E-4
86-87	1.6861887985129163E-5
88-89	2.5292831977693743E-4
90-91	0.0
92-93	0.0
94-95	5.058566395538749E-5
96-97	3.203758717174541E-4
98-99	4.046853116430999E-4
100	7.419230713456832E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2965267.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.731693186497836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.94834614975193	18.84322687954872
2	15.019868736989588	8.33052783031332
3	6.475905666775114	5.387634871671305
4	3.378981268359401	3.748194872682649
5	2.0707669733441354	2.8712937182756155
6	1.2548109028304588	2.0878818578619978
7	0.8684773112334987	1.6859042434193383
8	0.6131074993888194	1.360200725071332
9	0.4428992451275599	1.105411138056809
>10	1.73229099204822	7.641326939692962
>50	0.05419259505551867	1.0283118472562625
>100	0.08618307640064565	5.979318472720546
>500	0.02806969285113921	5.563996724762514
>1k	0.020682931574523628	10.551621155805691
>5k	0.0022160283829846745	4.34092119756185
>10k+	0.0032009298865334187	19.474227525299064
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	71239	2.402448076345233	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	53639	1.8089096192686864	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	53256	1.7959934130720774	No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	46339	1.5627260546858008	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	32779	1.1054316525290977	No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG	29130	0.982373594013625	No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT	23157	0.7809414801432721	No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA	22891	0.7719709557351834	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	21520	0.7257356588799592	No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC	19901	0.6711368655841109	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	17728	0.5978551004007396	No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG	14153	0.47729260130706613	No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG	14002	0.4722003111355571	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	13767	0.46427522378254643	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	13054	0.44023017151575217	No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT	12190	0.41109282907744904	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	12169	0.4103846297820736	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	11885	0.4008070774065202	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	11799	0.397906832673078	No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG	11335	0.3822590006228781	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11141	0.37571658808464803	No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG	10953	0.36937651820223943	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	10934	0.36873576645880457	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	10832	0.3652959413098382	No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA	10513	0.3545380567753258	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10096	0.34047524219572806	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	9881	0.3332246303621225	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	9867	0.3327524974985389	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	9247	0.31184375639697876	No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT	8942	0.30155800472605	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	8521	0.2873602950425712	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	8368	0.2822005573191217	No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA	7948	0.2680365714116132	No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA	7193	0.24257512055406816	No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC	6416	0.21637174662517744	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	6345	0.21397735853128907	No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT	6343	0.21390991097934858	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	5894	0.1987679355687026	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	5826	0.19647471880272502	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	5656	0.1907416768877811	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	5280	0.17806153712296396	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5234	0.17651024342833208	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	5134	0.17313786583130625	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	5051	0.1703387924257748	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	4767	0.16076124005022147	No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC	4743	0.15995186942693523	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	4708	0.15877153726797621	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	4556	0.15364552332049694	No Hit
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT	4088	0.13786279616641606	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	4081	0.13762672973462423	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	3984	0.13435552346550916	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	3814	0.12862248155056527	No Hit
GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT	3744	0.12626181723264718	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	3658	0.12336157249920497	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	3638	0.12268709697979979	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	3595	0.1212369746130787	No Hit
GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC	3577	0.12062994664561402	No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	3478	0.11729129282455847	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	3362	0.1133793348120085	No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG	3308	0.11155825090961453	No Hit
ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT	3163	0.10666830339392708	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	3122	0.1052856285791465	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	3120	0.10521818102720598	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	3103	0.1046448768357116	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	3098	0.1044762579558603	No Hit
TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA	3032	0.10225048874182324	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	5.0585663955387494E-5	0.0
12-13	0.0	0.0	0.0	1.348951038810333E-4	0.0
14-15	0.0	0.0	0.0	1.348951038810333E-4	0.0
16-17	0.0	0.0	0.0	1.5175699186616246E-4	0.0
18-19	0.0	0.0	0.0	3.035139837323249E-4	0.0
20-21	0.0	0.0	0.0	5.901660794795207E-4	0.0
22-23	0.0	0.0	0.0	0.0015512936946318829	0.0
24-25	0.0	0.0	0.0	0.0036590296927730285	0.0
26-27	0.0	0.0	0.0	0.006761617082036795	0.0
28-29	0.0	0.0	0.0	0.01595134603393219	0.0
30-31	0.0	0.0	0.0	0.04048539305229512	0.0
32-33	0.0	0.0	0.0	0.09779895031374915	0.0
34-35	0.0	0.0	0.0	0.20188738484595148	0.0
36-37	0.0	0.0	0.0	0.33853612507743824	0.0
38-39	0.0	0.0	0.0	0.48322798587783156	0.0
40-41	0.0	0.0	0.0	0.6357943483672803	0.0
42-43	0.0	0.0	0.0	0.7856965325550784	0.0
44-45	0.0	0.0	0.0	0.9571144858119016	0.0
46-47	0.0	0.0	0.0	1.178578522608588	0.0
48-49	0.0	0.0	0.0	1.4071582761349988	0.0
50-51	0.0	0.0	0.0	1.6125360717938721	0.0
52-53	0.0	0.0	0.0	1.8028224777060546	0.0
54-55	0.0	0.0	0.0	2.010662109010757	0.0
56-57	0.0	0.0	0.0	2.252984301245048	0.0
58-59	0.0	0.0	0.0	2.547999893432868	0.0
60-61	0.0	0.0	0.0	2.9009529327375914	0.0
62-63	0.0	0.0	0.0	3.19182050048107	0.0
64-65	0.0	0.0	0.0	3.454343234521546	0.0
66-67	0.0	0.0	0.0	3.7526637567544507	0.0
68-69	0.0	0.0	0.0	4.071420887225333	0.0
70-71	0.0	0.0	0.0	4.420225902085715	0.0
72-73	0.0	0.0	0.0	4.780851100423671	0.0
74-75	0.0	0.0	0.0	5.134478615247801	0.0
76-77	0.0	0.0	0.0	5.478427406368466	0.0
78-79	0.0	1.6861887985129163E-5	0.0	5.883770331642985	0.0
80-81	0.0	3.372377597025833E-5	0.0	6.299955450891943	0.0
82-83	0.0	3.372377597025833E-5	0.0	6.708518996771623	0.0
84-85	0.0	3.372377597025833E-5	0.0	7.238302655376396	0.0
86-87	0.0	3.372377597025833E-5	0.0	7.785454058605852	0.0
88	0.0	3.372377597025833E-5	0.0	8.141459099635885	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1820	0.0	28.447615	1
GGGCACC	1565	0.0	27.327465	94
TCCGCAC	70	0.008824062	26.865982	3
TAACTCG	35	0.0088407425	26.856014	28-29
AGGGCTA	1810	0.0	25.97452	5
TATTCGC	185	1.0059011E-8	25.403482	9
TAGGCAT	2210	0.0	25.315168	5
GTGATCG	1150	0.0	24.928549	8
GTATAAC	170	1.2279816E-7	24.918283	1
GAGTACT	11100	0.0	23.371204	12-13
GCTATTG	2040	0.0	23.03747	8
CTATTGA	2085	0.0	22.765663	9
TATTAAG	600	0.0	22.747936	2
CTAGGCA	2720	0.0	22.642715	4
CATGGGG	6425	0.0	22.537395	4
CCCTACA	150	3.258854E-5	21.963524	2
ATGGGGG	5505	0.0	21.777548	5
ATAAGGT	1405	0.0	21.750927	3
GGGCTAT	2185	0.0	21.724123	6
ATTTAGG	2345	0.0	21.677313	1
>>END_MODULE