FastQCFastQC Report
Mon 16 Jan 2017
ERR1146538_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146538_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4181044
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37950.039.766351
GTATCAA59950.034.1132051
CAACGCA60450.031.5612895
ATCAACG62800.030.5310063
AACGCAG70900.027.5049786
TATCAAC75000.026.6295012
TCAACGC73500.026.2775384
GTACTAG16400.025.5134161
GTACATG131350.024.9473931
ACGCAGA76500.024.9372227
TACATGG126500.024.8154032
CGCAGAG76850.024.6416978
GTGATCG11450.024.2134118
ACATGGG128750.023.4330273
TGATCGC12250.023.015729
CATGGGG105550.020.6135584
CGACGAG7900.020.52015724-25
GTATAAG25900.020.3301581
GCATCGT16300.020.179948
GCAGAGT96050.019.1777739
Produced by FastQC (version 0.11.5)