##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146538_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4181044
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47122034592317	31.0	31.0	34.0	29.0	34.0
2	31.5276481185082	33.0	31.0	34.0	28.0	34.0
3	31.756795910303744	33.0	31.0	34.0	30.0	34.0
4	35.30977789279424	37.0	35.0	37.0	33.0	37.0
5	35.29710139381456	37.0	35.0	37.0	33.0	37.0
6	35.274795481702654	37.0	35.0	37.0	32.0	37.0
7	35.30601806630114	37.0	35.0	37.0	33.0	37.0
8	35.23068329345494	37.0	35.0	37.0	32.0	37.0
9	36.89754879403326	39.0	38.0	39.0	33.0	39.0
10-11	36.87529011892724	39.0	37.5	39.0	33.0	39.0
12-13	36.827505881306195	39.0	37.0	39.0	33.0	39.0
14-15	38.25272025360173	40.0	38.5	41.0	33.0	41.0
16-17	38.26336149535858	40.5	38.5	41.0	33.0	41.0
18-19	38.28584164624912	40.0	38.5	41.0	33.0	41.0
20-21	38.37614600563879	40.5	39.0	41.0	33.0	41.0
22-23	38.393010693023086	40.0	39.0	41.0	33.0	41.0
24-25	38.439939761456706	41.0	39.0	41.0	33.0	41.0
26-27	38.46617842337942	41.0	39.0	41.0	33.0	41.0
28-29	38.43488037915889	40.0	38.0	41.0	33.0	41.0
30-31	38.419631077788225	40.0	38.0	41.0	33.0	41.0
32-33	38.43732498390354	40.0	38.0	41.0	33.0	41.0
34-35	38.38070049011682	40.0	38.0	41.0	33.0	41.0
36-37	38.294648657129656	40.0	38.0	41.0	33.0	41.0
38-39	38.2572429278429	40.0	38.0	41.0	33.0	41.0
40-41	38.20059463617221	40.0	38.0	41.0	33.0	41.0
42-43	38.087041657538165	40.0	38.0	41.0	33.0	41.0
44-45	37.95107035467697	40.0	38.0	41.0	32.0	41.0
46-47	37.852505259451945	40.0	38.0	41.0	32.0	41.0
48-49	37.7440688258722	40.0	37.0	41.0	32.0	41.0
50-51	37.62721786711644	40.0	37.0	41.0	32.0	41.0
52-53	37.51350296720149	40.0	37.0	41.0	31.5	41.0
54-55	37.343563951969884	40.0	36.0	41.0	31.0	41.0
56-57	37.220523510395964	39.5	36.0	41.0	31.0	41.0
58-59	37.00640725139463	39.0	35.0	41.0	31.0	41.0
60-61	36.83453116016	39.0	35.0	41.0	31.0	41.0
62-63	36.60372899687255	39.0	35.0	41.0	31.0	41.0
64-65	36.356285415795675	38.0	35.0	40.0	30.0	41.0
66-67	36.095331333513826	37.0	35.0	40.0	30.0	41.0
68-69	35.797098762892716	37.0	35.0	40.0	30.0	41.0
70-71	35.52074182907427	36.5	35.0	39.0	30.0	41.0
72-73	35.19504566323626	36.0	34.5	39.0	29.5	41.0
74-75	34.86333485129552	35.5	34.0	38.5	29.0	40.0
76-77	34.54531846591426	35.0	34.0	37.0	29.0	39.5
78-79	34.25584961555057	35.0	34.0	37.0	29.0	39.0
80-81	33.97302420161089	35.0	34.0	36.5	29.0	39.0
82-83	33.716677939768154	35.0	34.0	36.0	29.0	37.5
84-85	33.48957449383455	35.0	34.0	36.0	29.0	37.0
86-87	33.29645346951622	35.0	34.0	35.5	28.0	37.0
88-89	33.153015132105764	35.0	34.0	35.0	28.0	36.0
90-91	32.995514158664676	35.0	34.0	35.0	28.0	36.0
92-93	32.90845551972187	35.0	34.0	35.0	28.0	36.0
94-95	32.814119033428014	35.0	33.5	35.0	27.5	36.0
96-97	32.747261569120056	35.0	33.0	35.0	27.0	35.5
98-99	32.68243182803147	35.0	33.0	35.0	27.0	35.0
100	31.791179188738507	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2586383321354475
1101	2	0.27828903008601813
1101	3	0.3372527234129947
1101	4	0.35165686851002675
1101	5	0.27546508505431433
1101	6	0.25210015662393914
1101	7	0.28828629941709494
1101	8	0.2657949978762346
1101	9	0.4249815089107969
1101	10-11	0.4282186878237013
1101	12-13	0.3900278908534176
1101	14-15	0.4747493511466985
1101	16-17	0.4865910803083153
1101	18-19	0.38367182797044563
1101	20-21	0.3982109546079329
1101	22-23	0.38760141877090604
1101	24-25	0.4293157809720114
1101	26-27	0.3923443105950781
1101	28-29	0.45717050827950345
1101	30-31	0.44875461975189523
1101	32-33	0.41496313300734045
1101	34-35	0.4590844087306394
1101	36-37	0.4486972832202838
1101	38-39	0.35725475302268705
1101	40-41	0.3688177591652462
1101	42-43	0.4010050236432292
1101	44-45	0.35447684203256813
1101	46-47	0.3240549495128917
1101	48-49	0.3498823937981115
1101	50-51	0.3060762540248305
1101	52-53	0.254850497598504
1101	54-55	0.3414809287864884
1101	56-57	0.2927316202343988
1101	58-59	0.331031189847387
1101	60-61	0.26724976173505155
1101	62-63	0.30943226043832084
1101	64-65	0.2872297932876151
1101	66-67	0.2689631549102742
1101	68-69	0.18675848609701262
1101	70-71	0.2106527485147609
1101	72-73	0.283021236954923
1101	74-75	0.26727763353293454
1101	76-77	0.2777384073365994
1101	78-79	0.2747823313933111
1101	80-81	0.20755419785150764
1101	82-83	0.22006782552188753
1101	84-85	0.2658040979520919
1101	86-87	0.16955102029702118
1101	88-89	0.12063862837293726
1101	90-91	0.22444922471431283
1101	92-93	0.2058727373408118
1101	94-95	0.15116268635650698
1101	96-97	0.14000683732690078
1101	98-99	0.15609551271951716
1101	100	0.19783377901624988
1102	1	0.15774256208761628
1102	2	0.2121770243604857
1102	3	0.25354450878045043
1102	4	0.25437641665203614
1102	5	0.24246666427790586
1102	6	0.16945960154059492
1102	7	0.2606824633742164
1102	8	0.24595079627277983
1102	9	0.40647534364263294
1102	10-11	0.3551141446568593
1102	12-13	0.3617775016264133
1102	14-15	0.4554192539664683
1102	16-17	0.4375733330709579
1102	18-19	0.36389123564404713
1102	20-21	0.33840518356786475
1102	22-23	0.2923208983723029
1102	24-25	0.331342849732998
1102	26-27	0.3679880344863875
1102	28-29	0.38378761848763077
1102	30-31	0.3464487859493417
1102	32-33	0.2921886109087666
1102	34-35	0.30651977433305433
1102	36-37	0.34797211753729584
1102	38-39	0.3126549365680802
1102	40-41	0.33298013307741314
1102	42-43	0.3104550509598454
1102	44-45	0.25924130141249435
1102	46-47	0.2092182873770767
1102	48-49	0.28818114600028366
1102	50-51	0.2279862279196223
1102	52-53	0.23169851396536245
1102	54-55	0.2648082389140427
1102	56-57	0.24513594132167782
1102	58-59	0.24773760608577788
1102	60-61	0.2327107710912486
1102	62-63	0.15498739159995267
1102	64-65	0.10394416940265216
1102	66-67	0.13015954329806334
1102	68-69	0.15763893612194124
1102	70-71	0.13809169855223047
1102	72-73	0.16273445027044886
1102	74-75	0.10009486998330885
1102	76-77	0.1318683380268908
1102	78-79	0.06239264890771068
1102	80-81	0.11945510007712556
1102	82-83	0.12668517283272962
1102	84-85	0.09154949706369564
1102	86-87	0.021150427500401747
1102	88-89	0.07802798409513656
1102	90-91	0.07990025583215044
1102	92-93	0.04234778118488691
1102	94-95	0.003713542895020794
1102	96-97	-0.006898812930266729
1102	98-99	-0.021264784969908135
1102	100	-0.004374284787093785
1103	1	0.11345654256591331
1103	2	0.15117648064337175
1103	3	0.20267742763805074
1103	4	0.18228091699685933
1103	5	0.1587594168588211
1103	6	0.21922535153147038
1103	7	0.23321345785540615
1103	8	0.27556079649860976
1103	9	0.40569016749044806
1103	10-11	0.3586213806109768
1103	12-13	0.32423843824428644
1103	14-15	0.3376688878693983
1103	16-17	0.34487868494973384
1103	18-19	0.2515784749681771
1103	20-21	0.30081965234928987
1103	22-23	0.2698760648140066
1103	24-25	0.2762754235994862
1103	26-27	0.29847605968150503
1103	28-29	0.2696065902195528
1103	30-31	0.20791817710776428
1103	32-33	0.28792616404278704
1103	34-35	0.30096143939288567
1103	36-37	0.2834091844527151
1103	38-39	0.26716162185662995
1103	40-41	0.26852446686761056
1103	42-43	0.23696985899991319
1103	44-45	0.2417120701034179
1103	46-47	0.21244020212987635
1103	48-49	0.13105688839996787
1103	50-51	0.20285364258817395
1103	52-53	0.24182502807855855
1103	54-55	0.2719838282738607
1103	56-57	0.21687785114107072
1103	58-59	0.24071922566793091
1103	60-61	0.15079796386066846
1103	62-63	0.21631060449907835
1103	64-65	0.11799309004600644
1103	66-67	0.15926795145764316
1103	68-69	0.17053310931581933
1103	70-71	0.13241654260269797
1103	72-73	0.1709497031857694
1103	74-75	0.1603975275961318
1103	76-77	0.19202073515926088
1103	78-79	0.2304646419175569
1103	80-81	0.258694451994117
1103	82-83	0.17120049158472028
1103	84-85	0.16327542542158113
1103	86-87	0.15836951422936352
1103	88-89	0.13131139273242098
1103	90-91	0.18200963503922907
1103	92-93	0.1739863847525811
1103	94-95	0.11527977823593716
1103	96-97	0.06923547998560053
1103	98-99	0.041552347156823544
1103	100	0.012004107582107792
1104	1	0.06603002257342894
1104	2	0.06780497410987962
1104	3	0.11230265466650735
1104	4	0.05957461900040073
1104	5	0.13865383362306005
1104	6	0.1333800112727701
1104	7	0.07022416342730509
1104	8	0.14321919919332515
1104	9	0.23449320194433199
1104	10-11	0.23028546362636604
1104	12-13	0.2245147268341725
1104	14-15	0.2807954907416601
1104	16-17	0.2652993796005134
1104	18-19	0.17085790229840114
1104	20-21	0.15449330007140816
1104	22-23	0.13387420206083078
1104	24-25	0.1476747071064608
1104	26-27	0.1538302871809094
1104	28-29	0.15495517773690892
1104	30-31	0.21307923089102587
1104	32-33	0.21573328012124193
1104	34-35	0.1660546277843764
1104	36-37	0.19112284362802967
1104	38-39	0.14823165563464613
1104	40-41	0.19582799986225297
1104	42-43	0.13144861601617208
1104	44-45	0.09891185118599566
1104	46-47	0.06062581817108992
1104	48-49	0.0989800163602439
1104	50-51	0.07993429853863177
1104	52-53	0.048264630404140973
1104	54-55	0.07015942771683825
1104	56-57	0.07846127053424112
1104	58-59	0.018251228668333397
1104	60-61	0.048787881928731736
1104	62-63	0.020054077624273248
1104	64-65	0.0326852061727223
1104	66-67	-0.020734618582444853
1104	68-69	-0.035524494971262754
1104	70-71	-0.030677619855012495
1104	72-73	-0.02365891076699711
1104	74-75	-0.02674649339182622
1104	76-77	-0.08199685866452455
1104	78-79	-0.04014464814011376
1104	80-81	-0.026189851656177154
1104	82-83	0.003255950748304315
1104	84-85	0.01940926474470217
1104	86-87	-0.04880637302841251
1104	88-89	-0.05431504247472674
1104	90-91	0.039687045141654664
1104	92-93	0.030164924460009956
1104	94-95	-0.02961789407739701
1104	96-97	-0.003819889344129024
1104	98-99	0.007897597952890578
1104	100	-0.024591370087748032
1105	1	0.11163812143045249
1105	2	0.1450551662487065
1105	3	0.1305876764178997
1105	4	0.16044442372162848
1105	5	0.16355304060540732
1105	6	0.20643452575939847
1105	7	0.18192163011563167
1105	8	0.15284222828707072
1105	9	0.25560496923819187
1105	10-11	0.29308257141214966
1105	12-13	0.21160297469234735
1105	14-15	0.3555285387115603
1105	16-17	0.3151857050663409
1105	18-19	0.2448214821779473
1105	20-21	0.28551732016337894
1105	22-23	0.20501987889154094
1105	24-25	0.24472312837335863
1105	26-27	0.2732902652560938
1105	28-29	0.3357363087911054
1105	30-31	0.28961124140252537
1105	32-33	0.2689208792712634
1105	34-35	0.2689641961155118
1105	36-37	0.25536338866336195
1105	38-39	0.26957285996630276
1105	40-41	0.2641010072319787
1105	42-43	0.2380235405754334
1105	44-45	0.214580909623578
1105	46-47	0.19572120756940592
1105	48-49	0.18223893963433113
1105	50-51	0.17779303451158057
1105	52-53	0.1511504095750098
1105	54-55	0.1942407211534558
1105	56-57	0.17776361490179227
1105	58-59	0.1473289873894288
1105	60-61	0.11266810961096496
1105	62-63	0.19385226293920965
1105	64-65	0.1607580582796757
1105	66-67	0.15316568327175872
1105	68-69	0.14655420071186143
1105	70-71	0.15969801611994683
1105	72-73	0.13903612611818517
1105	74-75	0.1694889775354227
1105	76-77	0.151041013678622
1105	78-79	0.10999570220062083
1105	80-81	0.12001981059935929
1105	82-83	0.17117792823565736
1105	84-85	0.1928871235970533
1105	86-87	0.1700804289052087
1105	88-89	0.16140118353531108
1105	90-91	0.10599234096242327
1105	92-93	0.07995557984966695
1105	94-95	0.10162707425849504
1105	96-97	0.03779916096977587
1105	98-99	0.07573161830043773
1105	100	0.04129079815102088
1106	1	0.08076061168489446
1106	2	0.07610601911440895
1106	3	0.1508690014897489
1106	4	0.09441793137541055
1106	5	0.09262069156367403
1106	6	0.08164829348073965
1106	7	0.11456837153049548
1106	8	0.13759389545028
1106	9	0.12588227772706517
1106	10-11	0.1869254647380032
1106	12-13	0.13618802904335325
1106	14-15	0.1810906746876313
1106	16-17	0.13679116928432933
1106	18-19	0.13483526119522793
1106	20-21	0.07109926678175782
1106	22-23	0.1505486007225798
1106	24-25	0.15676752747904033
1106	26-27	0.13517631873492775
1106	28-29	0.14691671502177428
1106	30-31	0.13370079455147987
1106	32-33	0.14415267661226494
1106	34-35	0.17420200595494606
1106	36-37	0.17856069672020425
1106	38-39	0.16824451297134146
1106	40-41	0.14402298088860022
1106	42-43	0.13414590099412038
1106	44-45	0.03297707341555878
1106	46-47	0.10920327898312365
1106	48-49	0.0625383820831118
1106	50-51	0.08962310334928247
1106	52-53	0.12423694094846383
1106	54-55	0.13948153161663868
1106	56-57	0.003922697239715944
1106	58-59	0.032016349846777814
1106	60-61	0.10193877014872754
1106	62-63	0.0807134181039757
1106	64-65	0.05846019028641791
1106	66-67	0.07197234296884858
1106	68-69	0.07139740771372516
1106	70-71	0.06218519793232247
1106	72-73	0.08752339321262781
1106	74-75	0.07652707724388819
1106	76-77	0.0863315836418721
1106	78-79	0.03333918800059621
1106	80-81	0.038531442713662045
1106	82-83	0.047277791289296545
1106	84-85	0.10585103330517143
1106	86-87	0.0901073647631847
1106	88-89	0.04290433514657366
1106	90-91	0.09608198501891962
1106	92-93	0.06630156043145519
1106	94-95	0.04228335466414279
1106	96-97	0.06796403663796724
1106	98-99	0.03075854628276886
1106	100	-0.025516115142092843
1107	1	0.17024781397599043
1107	2	0.15570613866173133
1107	3	0.20207472327198062
1107	4	0.21117906154802313
1107	5	0.2708948740367063
1107	6	0.23879996781123225
1107	7	0.18229448754407684
1107	8	0.22996481430428872
1107	9	0.2531419683315761
1107	10-11	0.3356249486462488
1107	12-13	0.27238867235452346
1107	14-15	0.35866978795338156
1107	16-17	0.3576629601238466
1107	18-19	0.27633900391962385
1107	20-21	0.3093710712860087
1107	22-23	0.3163143047635586
1107	24-25	0.2979762188595316
1107	26-27	0.36491778047195567
1107	28-29	0.3407928346805562
1107	30-31	0.3366589037494876
1107	32-33	0.3440970061872832
1107	34-35	0.31156319727532633
1107	36-37	0.2616274787100892
1107	38-39	0.2758734605235773
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2216	50-51	-0.3822646087235171
2216	52-53	-0.34359760428490915
2216	54-55	-0.3199342710631825
2216	56-57	-0.298824492826526
2216	58-59	-0.2789001795074384
2216	60-61	-0.3088172040343977
2216	62-63	-0.3162165353737336
2216	64-65	-0.2978998258011103
2216	66-67	-0.28237862359248567
2216	68-69	-0.2841116688772303
2216	70-71	-0.3893420987749181
2216	72-73	-0.3248330398492243
2216	74-75	-0.29469716975905413
2216	76-77	-0.27312734805073546
2216	78-79	-0.35212840092462017
2216	80-81	-0.3307658221153531
2216	82-83	-0.3061670876788156
2216	84-85	-0.2830075087712274
2216	86-87	-0.27772520154112357
2216	88-89	-0.38747723147843516
2216	90-91	-0.3585270571735961
2216	92-93	-0.31852273103919515
2216	94-95	-0.28675536308295335
2216	96-97	-0.3241996079906144
2216	98-99	-0.2671654407238151
2216	100	-0.3214198483917663
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	100.0
1	77.0
2	63.0
3	43.0
4	46.0
5	61.0
6	116.0
7	389.0
8	1459.0
9	2171.0
10	2538.0
11	3494.0
12	4909.0
13	6229.0
14	7225.0
15	8348.0
16	9172.0
17	10192.0
18	11481.0
19	12579.0
20	14335.0
21	15880.0
22	18203.0
23	21155.0
24	25839.0
25	32371.0
26	36573.0
27	34218.0
28	37480.0
29	46677.0
30	53827.0
31	58979.0
32	74976.0
33	101168.0
34	149420.0
35	231018.0
36	430588.0
37	980929.0
38	1308339.0
39	427403.0
40	974.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.456020606482106	19.953279784496363	13.243465164816676	24.347234444204858
2	14.816010807950772	20.814394782025467	40.514219640790635	23.855374769233123
3	17.613974237839518	26.351030366817763	30.671873318104076	25.363122077238643
4	12.14414471304817	17.64568132282392	40.134984375568095	30.075189588559816
5	12.944943796573064	38.596201245392926	35.77155804242909	12.687296915604918
6	29.632136847982142	38.70370733606951	18.689651675077897	12.974504140870447
7	26.119202358139017	32.94152201762098	23.656086549289103	17.283189074950897
8	25.04194175370348	35.70870919857884	21.1413706187192	18.107978428998482
9	26.011312375754386	15.596124218468013	20.998026421695155	37.39453698408245
10-11	24.14358544794898	26.56119341437792	29.44254658979359	19.852674547879516
12-13	24.729757818324636	24.43129726532349	28.64587456859199	22.193070347759882
14-15	22.350466445255517	25.760723588444122	25.997519260653085	25.891290705647275
16-17	22.147854084619095	28.4077621343298	26.83994762988388	22.604436151167224
18-19	22.012520848746398	28.37728164159794	27.918962032019945	21.691235477635722
20-21	23.292101516664946	27.61795007214013	27.21511584518766	21.874832566007264
22-23	23.172104953356218	27.40826650829729	26.923459611862683	22.496168926483808
24-25	22.743899821126238	27.53903692319431	27.33264504148228	22.384418214197176
26-27	22.679326534512466	27.90309467777038	26.87181447289206	22.545764314825096
28-29	22.523448246664575	27.959915834350486	27.00671378201747	22.509922136967468
30-31	22.96175210025943	27.51850538932239	27.084879136713365	22.434863373704815
32-33	22.78161485643715	27.90824004685423	26.668365774451697	22.641779322256923
34-35	22.98682459152837	27.81240570178088	27.002453144063338	22.19831656262741
36-37	22.973968953543224	27.584039101350232	26.92785665007143	22.51413529503511
38-39	22.807964763189133	27.650287211106892	27.136710094700423	22.405037931003548
40-41	22.909680035308426	27.32396320364184	27.20525176990674	22.56110499114299
42-43	22.648899174738403	27.39636922217901	27.15011727678625	22.804614326296328
44-45	22.778425318391413	27.724431436214775	27.033831764163796	22.463311481230015
46-47	22.707859602726007	27.54685888271729	26.859818166397737	22.885463348158964
48-49	22.870740065570992	27.398504860763616	27.097290276953533	22.633464796711856
50-51	22.755099745963722	27.515875472938607	26.913645848834776	22.815378932262895
52-53	22.694262833874525	27.616523009731907	26.88191980133056	22.807294355063
54-55	22.75250063763089	27.4952422925618	26.93460562828296	22.81765144152434
56-57	22.861513231799773	27.439352506400976	26.886443371945372	22.81269088985388
58-59	22.75300130384585	27.486455806277142	27.085061735587622	22.675481154289383
60-61	22.747486580080054	27.614224092295885	26.966565135735266	22.671724191888796
62-63	22.8167867366124	27.376521828693406	26.90942077052355	22.897270664170644
64-65	22.44701719849048	27.493276288152174	26.99346900943652	23.06623750392082
66-67	22.81172049464372	27.458028509641085	26.79526315556509	22.934987840150104
68-69	22.638653791329233	27.38937048912269	27.01903126791894	22.952944451629133
70-71	22.671967084420107	27.177007139030568	27.003011662625838	23.14801411392349
72-73	22.937081793214233	27.29186350563128	27.033944149214246	22.737110551940244
74-75	22.751897235603668	27.61686679417787	26.902056752215938	22.72917921800253
76-77	22.807858324391855	27.417821043540826	26.78886893297905	22.985451699088273
78-79	22.76069680648902	27.47879647909759	26.809897277304863	22.950609437108533
80-81	22.625101750077402	27.54011501287997	26.985843280235866	22.848939956806763
82-83	23.041109033507638	27.193919361071135	27.278403252067534	22.486568353353693
84-85	23.087935240417604	27.40963105948736	26.85716773964481	22.64526596045022
86-87	22.580269691185254	27.667588621354156	26.97730091813863	22.774840769321962
88-89	22.995903267493176	27.225094439451507	26.91658644940559	22.862415843649732
90-91	22.934608033154507	27.246756527915977	26.98054144038464	22.838093998544874
92-93	23.110990313617435	27.312450227358614	26.95698818352484	22.619571275499116
94-95	22.604794197951687	27.34368635212506	27.176321414373696	22.875198035549555
96-97	22.67549079139589	27.349786275402078	27.050409146350507	22.92431378685152
98-99	22.672504912921546	27.4969173121929	26.938145755508526	22.892432019377033
100	22.925201608851552	27.269062527213727	26.69543307026333	23.11030279367139
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1303.0
1	975.5
2	670.5
3	693.0
4	630.5
5	548.0
6	485.5
7	439.5
8	445.0
9	466.5
10	426.0
11	388.0
12	413.5
13	430.5
14	456.5
15	578.0
16	721.0
17	856.0
18	1097.5
19	1425.5
20	1993.0
21	3023.5
22	4486.0
23	6633.5
24	9511.0
25	12999.0
26	18747.0
27	25749.0
28	32176.5
29	39468.5
30	48289.0
31	57161.5
32	67751.5
33	77942.0
34	86546.0
35	94754.5
36	104313.5
37	116845.0
38	122937.5
39	123925.0
40	126346.5
41	129022.5
42	132677.0
43	140174.5
44	148154.0
45	158232.0
46	167153.0
47	171717.0
48	175122.0
49	173170.5
50	168111.0
51	164683.0
52	160369.0
53	154161.0
54	146368.5
55	133663.0
56	120860.5
57	106263.5
58	92207.0
59	82570.0
60	68212.5
61	51810.0
62	39493.0
63	29904.5
64	21557.5
65	14308.5
66	9924.0
67	7509.5
68	5448.0
69	3873.0
70	2791.0
71	2106.0
72	1560.0
73	1077.0
74	752.5
75	509.0
76	319.5
77	202.5
78	134.0
79	95.0
80	71.0
81	53.5
82	53.5
83	43.0
84	29.5
85	21.5
86	17.5
87	14.5
88	8.5
89	6.0
90	8.0
91	8.0
92	7.5
93	6.5
94	3.0
95	2.0
96	3.0
97	4.0
98	5.0
99	4.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06106130430581452
2	0.009997503015993135
3	0.011815230837082795
4	0.009853998188012372
5	0.012341415206345593
6	0.012484920034326356
7	0.002870096559615254
8	0.005883697947211271
9	0.0074861685263297876
10-11	0.007175241399038135
12-13	0.006146790131842669
14-15	0.01041605876427036
16-17	0.009937709337667817
18-19	0.008060187838252839
20-21	0.006983901628397118
22-23	0.0024156646043428385
24-25	0.0038865890911456563
26-27	0.005285761163958093
28-29	0.005764110590560635
30-31	0.008849464392147033
32-33	0.010009461751658198
34-35	0.011731519687427352
36-37	0.01741191912833254
38-39	0.018093567061241163
40-41	0.01767501131296394
42-43	0.023331493282539
44-45	0.026931072717723135
46-47	0.0295022008857118
48-49	0.03108075399350019
50-51	0.031714566983748554
52-53	0.02957395329970218
54-55	0.03611538170849194
56-57	0.03838754148485402
58-59	0.03552940366090383
60-61	0.036581772399429426
62-63	0.03175044319074374
64-65	0.034273736416072155
66-67	0.03612734044415701
68-69	0.03904527194643252
70-71	0.03886589091145656
72-73	0.03451291112937343
74-75	0.036880740791056014
76-77	0.037131874240022344
78-79	0.03564899101755447
80-81	0.04032485666259432
82-83	0.037048163090366903
84-85	0.03348445986217796
86-87	0.03198961790404502
88-89	0.02988488042699383
90-91	0.032073329053700465
92-93	0.03380734572513468
94-95	0.0371797091826826
96-97	0.03267126583695364
98-99	0.029896839162658895
100	0.027935606513588472
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4181044.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.20110510239873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.34379774995818	37.58510470044964
2	14.811203193134032	16.055671619280826
3	6.461423577166448	10.506488952513473
4	3.2951600441776927	7.144052635347997
5	1.8803306113528442	5.095799854659659
6	1.1749987231105152	3.82117375718984
7	0.7293926810555857	2.767372255676994
8	0.5301462747861734	2.2987611167464435
9	0.3808186942642591	1.8576714665497822
>10	1.3608709622461987	11.373114692428734
>50	0.02570684034080499	0.919852071649829
>100	0.005989147427285282	0.499043758661292
>500	1.615009798942899E-4	0.07589311884533816
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.175241399038135E-5
2	0.0	0.0	0.0	0.0	7.175241399038135E-5
3	0.0	0.0	0.0	0.0	7.175241399038135E-5
4	0.0	0.0	0.0	0.0	7.175241399038135E-5
5	0.0	0.0	0.0	0.0	7.175241399038135E-5
6	0.0	0.0	0.0	0.0	9.566988532050847E-5
7	0.0	0.0	0.0	0.0	1.435048279807627E-4
8	0.0	0.0	0.0	0.0	1.435048279807627E-4
9	0.0	0.0	0.0	0.0	1.435048279807627E-4
10-11	0.0	0.0	0.0	0.0	1.6742229931088982E-4
12-13	0.0	0.0	0.0	0.0	5.022668979326694E-4
14-15	0.0	0.0	0.0	0.0	6.457717259134322E-4
16-17	0.0	0.0	0.0	0.0	7.534003468990042E-4
18-19	0.0	0.0	0.0	0.0	8.131940252243219E-4
20-21	0.0	0.0	0.0	1.1958735665063558E-5	9.088639105448305E-4
22-23	0.0	0.0	0.0	1.435048279807627E-4	9.806163245352118E-4
24-25	0.0	0.0	0.0	4.663906909374788E-4	9.925750602002752E-4
26-27	0.0	0.0	0.0	8.729877035496398E-4	0.0012078323021714192
28-29	0.0	0.0	0.0	0.002762467938629682	0.0012197910378364829
30-31	0.0	0.0	0.0	0.008454826115199935	0.0012317497735015465
32-33	0.0	0.0	0.0	0.021968197416721756	0.0012676259804967373
34-35	0.0	0.0	0.0	0.043386292992850586	0.0013393783944871185
36-37	0.0	0.0	0.0	0.07880806803276885	0.0013393783944871185
38-39	0.0	0.0	0.0	0.12730074115460158	0.0013632958658172456
40-41	0.0	0.0	0.0	0.17819712014511208	0.0013872133371473727
42-43	0.0	0.0	0.0	0.2283042225817284	0.001435048279807627
44-45	0.0	0.0	0.0	0.28980800010715024	0.0014828832224678813
46-47	0.0	0.0	0.0	0.36445442812847695	0.0015785531077883897
48-49	0.0	0.0	0.0	0.4431787850115904	0.0017698928784294066
50-51	0.0	0.0	0.0	0.5216639671814025	0.0019373151777402964
52-53	0.0	0.0	0.0	0.5974703925622404	0.002104737477051186
54-55	0.0	0.0	0.0	0.6783114456580701	0.002104737477051186
56-57	0.0	0.0	0.0	0.774065042128234	0.0021764898910415676
58-59	0.0	0.0	0.0	0.8778788264366508	0.002272159776362076
60-61	0.0	0.0	0.0	0.987074041794346	0.0023080359833572667
62-63	0.0	0.0	0.0	1.0954919393338123	0.002463499547003093
64-65	0.0	0.0	0.0	1.2090162170022607	0.002463499547003093
66-67	0.0	0.0	0.0	1.3350134559693703	0.00248741701833322
68-69	0.0	0.0	0.0	1.4737347896841078	0.0025113344896633472
70-71	0.0	0.0	0.0	1.6232070267617371	0.002547210696658538
72-73	0.0	0.0	0.0	1.772786892460352	0.002678756788974237
74-75	0.0	0.0	0.0	1.922546139193943	0.002786385409959809
76-77	0.0	0.0	0.0	2.088377926661379	0.002870096559615254
78-79	0.0	0.0	0.0	2.2658814401379175	0.002870096559615254
80-81	0.0	0.0	0.0	2.4581539921608098	0.002894014030945381
82-83	0.0	0.0	0.0	2.656190654774262	0.00319298242257197
84-85	0.0	0.0	0.0	2.853019485085543	0.0032049411582370335
86-87	0.0	0.0	0.0	3.058805408409957	0.0033364872505527326
88	0.0	0.0	0.0	3.2218747279387636	0.0034680333428684317
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3795	0.0	39.76635	1
GTATCAA	5995	0.0	34.113205	1
CAACGCA	6045	0.0	31.561289	5
ATCAACG	6280	0.0	30.531006	3
AACGCAG	7090	0.0	27.504978	6
TATCAAC	7500	0.0	26.629501	2
TCAACGC	7350	0.0	26.277538	4
GTACTAG	1640	0.0	25.513416	1
GTACATG	13135	0.0	24.947393	1
ACGCAGA	7650	0.0	24.937222	7
TACATGG	12650	0.0	24.815403	2
CGCAGAG	7685	0.0	24.641697	8
GTGATCG	1145	0.0	24.213411	8
ACATGGG	12875	0.0	23.433027	3
TGATCGC	1225	0.0	23.01572	9
CATGGGG	10555	0.0	20.613558	4
CGACGAG	790	0.0	20.520157	24-25
GTATAAG	2590	0.0	20.330158	1
GCATCGT	1630	0.0	20.17994	8
GCAGAGT	9605	0.0	19.177773	9
>>END_MODULE