FastQCFastQC Report
Mon 16 Jan 2017
ERR1146538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4181044
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126360.30222116772748625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115450.27612720650631756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64800.1549852142192237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75100.059.970111
GTATCAA141200.043.6615751
ATCAACG175050.034.703093
CAACGCA175550.034.631035
TATCAAC184800.033.0779652
AACGCAG186050.032.9660386
TCAACGC185200.032.9281044
ACGCAGA203350.029.8595687
CGCAGAG204200.029.6892438
GCAGAGT231650.025.846539
GTACTAG15950.023.6043551
GAGTACT161350.023.27249712-13
AGAGTAC215150.022.96852310-11
GTACATG132000.022.8175411
TACATGG126250.022.8113842
CAGAGTA227700.022.67264410-11
GTGATCG11500.022.4765688
TGATCGC12500.021.8063589
ACATGGG130800.021.1367073
GTGGTAT24850.020.8319131
Produced by FastQC (version 0.11.5)