FastQCFastQC Report
Mon 16 Jan 2017
ERR1146537_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146537_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4526934
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44600.035.317821
GTATCAA67900.032.0623361
CAACGCA66400.030.291765
ATCAACG67300.029.816843
AACGCAG77150.027.0452526
TATCAAC81650.026.0154342
TCAACGC79150.025.768414
GTACATG133150.024.1192321
ACGCAGA82700.024.0358627
TACATGG131350.024.0071852
CGCAGAG83900.023.6368568
ACATGGG131150.023.5421373
GTACTAG19250.021.7391821
CATGGGG103800.021.3694554
GCAGAGT100600.019.1524859
ATGGGGG72000.018.9284175
GCATCGT19050.018.5014388
TATTCGC3901.8189894E-1118.0744849
TAGGCAT28350.018.0685065
GTATAAG24350.017.9584121
Produced by FastQC (version 0.11.5)