##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146537_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4526934
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.433599650447743	31.0	31.0	34.0	30.0	34.0
2	31.62575619613628	33.0	31.0	34.0	30.0	34.0
3	31.723080787128772	33.0	31.0	34.0	30.0	34.0
4	35.30303821526888	37.0	35.0	37.0	33.0	37.0
5	35.32709622009068	37.0	35.0	37.0	33.0	37.0
6	35.49949170895798	37.0	35.0	37.0	33.0	37.0
7	35.4751617761602	37.0	35.0	37.0	33.0	37.0
8	35.40107542986048	37.0	35.0	37.0	33.0	37.0
9	37.09240006591658	39.0	38.0	39.0	34.0	39.0
10-11	37.057494653114006	39.0	38.0	39.0	34.0	39.0
12-13	37.01839854524056	39.0	38.0	39.0	34.0	39.0
14-15	38.471962149216225	41.0	39.0	41.0	34.0	41.0
16-17	38.48100380964246	41.0	39.0	41.0	34.0	41.0
18-19	38.517084300323354	41.0	39.0	41.0	34.0	41.0
20-21	38.6096302707307	41.0	39.0	41.0	34.0	41.0
22-23	38.631068732170604	41.0	39.0	41.0	34.0	41.0
24-25	38.687003278598716	41.0	39.0	41.0	34.0	41.0
26-27	38.71821093481813	41.0	39.0	41.0	34.0	41.0
28-29	38.686232116483254	41.0	39.0	41.0	34.0	41.0
30-31	38.67153176962597	41.0	39.0	41.0	34.0	41.0
32-33	38.68771214689677	41.0	39.0	41.0	34.0	41.0
34-35	38.634914823145195	41.0	39.0	41.0	34.0	41.0
36-37	38.55587843781244	40.5	38.5	41.0	34.0	41.0
38-39	38.51968120144893	40.0	38.0	41.0	33.5	41.0
40-41	38.460720324175256	40.0	38.0	41.0	33.0	41.0
42-43	38.35050886538218	40.0	38.0	41.0	33.0	41.0
44-45	38.23085260355022	40.0	38.0	41.0	33.0	41.0
46-47	38.13786781517027	40.0	38.0	41.0	33.0	41.0
48-49	38.03323209483504	40.0	38.0	41.0	33.0	41.0
50-51	37.91564367848085	40.0	37.0	41.0	33.0	41.0
52-53	37.80630090918048	40.0	37.0	41.0	32.5	41.0
54-55	37.63396130802879	40.0	37.0	41.0	32.0	41.0
56-57	37.50924709748364	40.0	36.0	41.0	32.0	41.0
58-59	37.30417419383627	39.5	36.0	41.0	32.0	41.0
60-61	37.12982616932344	39.0	35.0	41.0	31.5	41.0
62-63	36.90387025744135	39.0	35.0	41.0	31.0	41.0
64-65	36.65686632497845	38.5	35.0	41.0	31.0	41.0
66-67	36.39714550731246	37.5	35.0	40.0	31.0	41.0
68-69	36.098292133262824	37.0	35.0	40.0	31.0	41.0
70-71	35.8184484244745	37.0	35.0	39.5	30.5	41.0
72-73	35.48573659346481	36.0	35.0	39.0	30.0	41.0
74-75	35.149514550024364	36.0	35.0	39.0	30.0	40.5
76-77	34.822473665399144	35.0	34.5	37.5	30.0	39.5
78-79	34.51916683565521	35.0	34.0	37.0	30.0	39.0
80-81	34.21736035913048	35.0	34.0	37.0	29.0	39.0
82-83	33.946465422292434	35.0	34.0	36.0	29.0	38.0
84-85	33.70882224039494	35.0	34.0	36.0	29.0	37.0
86-87	33.50089354074966	35.0	34.0	35.5	29.0	37.0
88-89	33.34798143732601	35.0	34.0	35.0	29.0	36.5
90-91	33.17780389552841	35.0	34.0	35.0	29.0	36.0
92-93	33.07880907916926	35.0	34.0	35.0	29.0	36.0
94-95	32.97833345924637	35.0	34.0	35.0	28.5	36.0
96-97	32.89796504654143	35.0	34.0	35.0	28.0	35.5
98-99	32.82212994490311	35.0	34.0	35.0	28.0	35.0
100	31.955886478574683	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.219621238798144
1101	2	0.14022084938098445
1101	3	0.18375560362237664
1101	4	0.25146350770651793
1101	5	0.2103457394135404
1101	6	0.2162370561884117
1101	7	0.3044094780379254
1101	8	0.3085081224271846
1101	9	0.42026230395349273
1101	10-11	0.4080407005328155
1101	12-13	0.37215265286408084
1101	14-15	0.49370754871386424
1101	16-17	0.43557169660085293
1101	18-19	0.3847558603085517
1101	20-21	0.34512556964873653
1101	22-23	0.34847977934793306
1101	24-25	0.35129215065762764
1101	26-27	0.38516481140684533
1101	28-29	0.4082082771489297
1101	30-31	0.42098736132751213
1101	32-33	0.4139066563120011
1101	34-35	0.41956433388867964
1101	36-37	0.41683119953010106
1101	38-39	0.39932095130449596
1101	40-41	0.3761870908269671
1101	42-43	0.3419602280417706
1101	44-45	0.32673842848596024
1101	46-47	0.2896749680025934
1101	48-49	0.31201741547833706
1101	50-51	0.3206850666429588
1101	52-53	0.2652542522863186
1101	54-55	0.34684698133762737
1101	56-57	0.27169749549798894
1101	58-59	0.3233981005767319
1101	60-61	0.3641002324758844
1101	62-63	0.3504307824500188
1101	64-65	0.2772483527595355
1101	66-67	0.30152259099713774
1101	68-69	0.27633310891769014
1101	70-71	0.2197617363033615
1101	72-73	0.22004883008738574
1101	74-75	0.23495099531559305
1101	76-77	0.244050945550228
1101	78-79	0.2456167799466229
1101	80-81	0.23791481804740755
1101	82-83	0.18747737945083998
1101	84-85	0.1795412857378622
1101	86-87	0.17431298737329115
1101	88-89	0.16235807394475898
1101	90-91	0.17672119397110464
1101	92-93	0.19863071017432787
1101	94-95	0.20753417336039348
1101	96-97	0.15515859792604658
1101	98-99	0.1571597635916291
1101	100	0.13813061643135782
1102	1	0.18257964539697014
1102	2	0.2196542019534995
1102	3	0.2549271472954189
1102	4	0.3206065626047163
1102	5	0.33235369147938343
1102	6	0.35793063144911486
1102	7	0.3472952212199303
1102	8	0.38864625353414795
1102	9	0.4605621375705482
1102	10-11	0.5034534977212886
1102	12-13	0.5006638669343246
1102	14-15	0.6039039021787005
1102	16-17	0.546574228236075
1102	18-19	0.49614902127515137
1102	20-21	0.509454592938269
1102	22-23	0.5094070126347887
1102	24-25	0.5292367960857192
1102	26-27	0.5440705152895617
1102	28-29	0.5891810658636132
1102	30-31	0.545438913310953
1102	32-33	0.49733022316311093
1102	34-35	0.46629303573406133
1102	36-37	0.5641853839356301
1102	38-39	0.5447763801096883
1102	40-41	0.5287926967240892
1102	42-43	0.46891891885137227
1102	44-45	0.48755362611749575
1102	46-47	0.4181236936353159
1102	48-49	0.45793977866469504
1102	50-51	0.4668911737314758
1102	52-53	0.4368151122019128
1102	54-55	0.4616514740855848
1102	56-57	0.3998988351987407
1102	58-59	0.42764569485213144
1102	60-61	0.4170443088988307
1102	62-63	0.4210875369496705
1102	64-65	0.40242971950407735
1102	66-67	0.39151176239140995
1102	68-69	0.35220260714852003
1102	70-71	0.36819672410914706
1102	72-73	0.4003470559001414
1102	74-75	0.35026810334544933
1102	76-77	0.3519628162815849
1102	78-79	0.35576218556559525
1102	80-81	0.35250831201332744
1102	82-83	0.295899224696619
1102	84-85	0.33846808658080363
1102	86-87	0.3382306782978617
1102	88-89	0.28409592364219094
1102	90-91	0.3689346210920448
1102	92-93	0.31864231496144413
1102	94-95	0.30904495498671736
1102	96-97	0.34963037731312596
1102	98-99	0.3582884363739751
1102	100	0.31840974409685785
1103	1	0.11175178682854892
1103	2	0.09583907845279782
1103	3	0.15405665546323632
1103	4	0.11361659836676097
1103	5	0.12259070102869174
1103	6	0.19263058326407645
1103	7	0.1992004674830099
1103	8	0.20710472405710334
1103	9	0.3078234955577983
1103	10-11	0.2621085578301958
1103	12-13	0.24571257582557848
1103	14-15	0.3136571652246758
1103	16-17	0.3162421281823242
1103	18-19	0.2557764709388479
1103	20-21	0.29442141252216913
1103	22-23	0.26484852002374737
1103	24-25	0.3170352589704848
1103	26-27	0.31503018089367174
1103	28-29	0.3629381112838814
1103	30-31	0.3473246935352776
1103	32-33	0.3244378864714932
1103	34-35	0.36807295989792266
1103	36-37	0.38704881072428776
1103	38-39	0.3138383876686035
1103	40-41	0.2998430531822365
1103	42-43	0.3029082086559498
1103	44-45	0.2566326320705059
1103	46-47	0.24084862070925084
1103	48-49	0.23736471471086418
1103	50-51	0.21120464242237347
1103	52-53	0.28539580384536833
1103	54-55	0.27024402614446785
1103	56-57	0.26703249496112846
1103	58-59	0.2770845457191413
1103	60-61	0.22353977006090275
1103	62-63	0.2793056302480892
1103	64-65	0.2595606174134346
1103	66-67	0.27998395122197905
1103	68-69	0.24760256097352595
1103	70-71	0.21088705811762765
1103	72-73	0.2539475235117905
1103	74-75	0.2398954249948133
1103	76-77	0.21984325437807684
1103	78-79	0.26625050914269366
1103	80-81	0.32231495310482217
1103	82-83	0.28790828103672794
1103	84-85	0.261359791622759
1103	86-87	0.2443232115476235
1103	88-89	0.20258853974192448
1103	90-91	0.2567125006200044
1103	92-93	0.2349127875690158
1103	94-95	0.18439518366634644
1103	96-97	0.21556439401090444
1103	98-99	0.18111070767834292
1103	100	0.1703461517429936
1104	1	0.08455939927365108
1104	2	0.08803271753198061
1104	3	0.12348105976008483
1104	4	0.11635011955441854
1104	5	0.11741419155581667
1104	6	0.10536164863555797
1104	7	0.16404998493834455
1104	8	0.17778250436318643
1104	9	0.19288646801425102
1104	10-11	0.2337034651152905
1104	12-13	0.1805297372117849
1104	14-15	0.21622403388407463
1104	16-17	0.19279588947640036
1104	18-19	0.17802604471345518
1104	20-21	0.15017962248523986
1104	22-23	0.19987476526760162
1104	24-25	0.18263066182001353
1104	26-27	0.2340075963214474
1104	28-29	0.23698202093356713
1104	30-31	0.2140984507120791
1104	32-33	0.24476284749604815
1104	34-35	0.21493896033197757
1104	36-37	0.23639472658873473
1104	38-39	0.20872027729502207
1104	40-41	0.203441251921177
1104	42-43	0.18776581412218718
1104	44-45	0.17996889980582864
1104	46-47	0.12590143551712885
1104	48-49	0.1868285746962286
1104	50-51	0.22164026438619544
1104	52-53	0.20643956946309316
1104	54-55	0.25242608818792434
1104	56-57	0.19367525845771638
1104	58-59	0.18381077690867897
1104	60-61	0.16029596886323816
1104	62-63	0.1633451223270157
1104	64-65	0.12959993113639712
1104	66-67	0.11988909086066712
1104	68-69	0.13665049839253385
1104	70-71	0.182781662999993
1104	72-73	0.14734042530402292
1104	74-75	0.12360050634979558
1104	76-77	0.14670540698212875
1104	78-79	0.16742748406935704
1104	80-81	0.15229839144122792
1104	82-83	0.12360033129596815
1104	84-85	0.12273930066938732
1104	86-87	0.1078506137570372
1104	88-89	0.08234849504934516
1104	90-91	0.09464621108075733
1104	92-93	0.09637524850936074
1104	94-95	0.02794988458988712
1104	96-97	0.08037471931302065
1104	98-99	0.019918069845424213
1104	100	0.023477905086519257
1105	1	-0.003254495468684837
1105	2	0.07170685128210508
1105	3	0.0508001104017346
1105	4	0.07009575328625317
1105	5	-0.021813557227915226
1105	6	0.049351708361875524
1105	7	0.07149771569172003
1105	8	0.10171364374139813
1105	9	0.13644003533502058
1105	10-11	0.18246949695869574
1105	12-13	0.13503878878393039
1105	14-15	0.21641812358936363
1105	16-17	0.21438994773821918
1105	18-19	0.16748207114816438
1105	20-21	0.19182234833742484
1105	22-23	0.16223814195684128
1105	24-25	0.20651173523173583
1105	26-27	0.23222856780725465
1105	28-29	0.2060529142771088
1105	30-31	0.21198375691231774
1105	32-33	0.20297404485285853
1105	34-35	0.18055366154623442
1105	36-37	0.17856010802221078
1105	38-39	0.1763834044846604
1105	40-41	0.19702530119082695
1105	42-43	0.1795105436608253
1105	44-45	0.15439044484090658
1105	46-47	0.1333285332207268
1105	48-49	0.14346511100858095
1105	50-51	0.1502813456210248
1105	52-53	0.11332182311726768
1105	54-55	0.15590909002833797
1105	56-57	0.13028643344850366
1105	58-59	0.14976954566849798
1105	60-61	0.05087521241527071
1105	62-63	0.12821042197099075
1105	64-65	0.1640125372671335
1105	66-67	0.13502318655405077
1105	68-69	0.10296241213953294
1105	70-71	0.11709985873668671
1105	72-73	0.09820310859425518
1105	74-75	0.1509959000258121
1105	76-77	0.10415093589326574
1105	78-79	0.12007417221229844
1105	80-81	0.12713040938325548
1105	82-83	0.0580293568431145
1105	84-85	0.06470899196710178
1105	86-87	0.04771672674478111
1105	88-89	0.04926574661708116
1105	90-91	0.09527519276110041
1105	92-93	0.05501033488172169
1105	94-95	0.07984298003523094
1105	96-97	0.062175186593712795
1105	98-99	0.10450600938646204
1105	100	0.003771529564161824
1106	1	0.01874976894156788
1106	2	0.019430567318671876
1106	3	0.02953001057599991
1106	4	0.028060561880444368
1106	5	0.04638990986778424
1106	6	0.04080380317083865
1106	7	0.024676357050182673
1106	8	0.033771055136270434
1106	9	0.12650098064299442
1106	10-11	0.07927746595564855
1106	12-13	0.08958830362888648
1106	14-15	0.11350616087629817
1106	16-17	0.12341161088438213
1106	18-19	0.11330730517143905
1106	20-21	0.10198675644763
1106	22-23	0.10227986476214568
1106	24-25	0.08218556816139255
1106	26-27	0.09868977864692852
1106	28-29	0.1370309082140153
1106	30-31	0.08725758238563941
1106	32-33	0.09857212210736321
1106	34-35	0.09502450358622383
1106	36-37	0.07863211824095373
1106	38-39	0.03854713974887147
1106	40-41	0.06439210003935614
1106	42-43	0.038550160890778784
1106	44-45	0.005006557703744363
1106	46-47	0.021686952048533215
1106	48-49	-0.0073152749467553235
1106	50-51	-0.017991961599236106
1106	52-53	0.004223468515846207
1106	54-55	0.015961550244718126
1106	56-57	-0.0036292372991084676
1106	58-59	0.006999119814729227
1106	60-61	0.014844232440452743
1106	62-63	-0.044931184359349174
1106	64-65	-0.033868913548047885
1106	66-67	-0.04484669282375364
1106	68-69	-0.055577800780106656
1106	70-71	-0.0703453545611481
1106	72-73	-0.10727672025188895
1106	74-75	-0.08797707826049361
1106	76-77	-0.05527076974843226
1106	78-79	-0.05772389793159505
1106	80-81	-0.014953306636492414
1106	82-83	-0.01573284503114536
1106	84-85	0.012182411350160294
1106	86-87	-0.044133198418506936
1106	88-89	-0.061174725971028465
1106	90-91	-0.007867117623469255
1106	92-93	-0.044320376772276404
1106	94-95	-0.058534628591992544
1106	96-97	-6.19303245180447E-4
1106	98-99	-0.08194873929117108
1106	100	-0.040779265846598634
1107	1	0.0688769854618343
1107	2	0.08992282908630145
1107	3	0.1117952615207507
1107	4	0.12971677815024663
1107	5	0.0962651265121508
1107	6	0.10977497523528257
1107	7	0.08920980768195363
1107	8	0.1361186881510008
1107	9	0.1620310184221765
1107	10-11	0.20053497156752798
1107	12-13	0.19842846108448953
1107	14-15	0.2575964743393513
1107	16-17	0.22645520586794277
1107	18-19	0.19094058854801688
1107	20-21	0.17774541763181162
1107	22-23	0.19600389649603756
1107	24-25	0.17920436711695942
1107	26-27	0.20642232609521471
1107	28-29	0.21390425729146045
1107	30-31	0.2078663646989014
1107	32-33	0.19038621005821454
1107	34-35	0.20023770805409669
1107	36-37	0.22391565473447628
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2216	38-39	-0.456584732714056
2216	40-41	-0.4623423422387489
2216	42-43	-0.38137723795121303
2216	44-45	-0.33681622380404974
2216	46-47	-0.36559852036790375
2216	48-49	-0.29811461688252905
2216	50-51	-0.30542969568484324
2216	52-53	-0.2769164373856583
2216	54-55	-0.2857013536776165
2216	56-57	-0.2694606416024783
2216	58-59	-0.23233453150387362
2216	60-61	-0.27901055764493776
2216	62-63	-0.28841847948996957
2216	64-65	-0.27440119184213785
2216	66-67	-0.30755931076264176
2216	68-69	-0.30294680698702336
2216	70-71	-0.33776522996965497
2216	72-73	-0.2582764242403002
2216	74-75	-0.29832273994437486
2216	76-77	-0.263457666439848
2216	78-79	-0.24223586640465555
2216	80-81	-0.20762618996866422
2216	82-83	-0.20521577190788065
2216	84-85	-0.18146449074959037
2216	86-87	-0.23542903797262227
2216	88-89	-0.21780618207809255
2216	90-91	-0.17231696898779347
2216	92-93	-0.1868523896490899
2216	94-95	-0.20503545300526582
2216	96-97	-0.19345987204334136
2216	98-99	-0.18172074171392438
2216	100	-0.23816690525316275
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	141.0
1	72.0
2	60.0
3	39.0
4	52.0
5	73.0
6	169.0
7	908.0
8	2600.0
9	3232.0
10	3073.0
11	3248.0
12	3901.0
13	4694.0
14	6035.0
15	6947.0
16	7704.0
17	8538.0
18	9728.0
19	11177.0
20	12780.0
21	14486.0
22	16954.0
23	20016.0
24	25429.0
25	32108.0
26	36185.0
27	34115.0
28	36417.0
29	47128.0
30	53500.0
31	58013.0
32	74673.0
33	101031.0
34	152619.0
35	237513.0
36	450284.0
37	1048106.0
38	1475300.0
39	526722.0
40	1164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.07995954213473	20.265159929472816	13.227913893532422	24.42696663486003
2	15.034703405566463	20.992793329770613	40.31716771961389	23.655335545049034
3	17.846588900671616	26.06358253799929	30.78150406504065	25.308324496288442
4	12.03567713925422	18.017914124958796	40.243973026304744	29.702435709482238
5	13.178274022362599	38.36526049305883	35.82032243305216	12.636143051526414
6	29.47662023484706	38.90616059297226	18.881549151081998	12.735670021098677
7	25.933745206599724	33.09138890036377	23.53783480902722	17.437031084009288
8	24.969994439610126	35.39597950895815	21.46186780940742	18.17215824202431
9	25.962737996220113	15.607890699300468	21.18959173506797	37.239779569411446
10-11	23.87776120659241	26.53734072755285	29.704497791830025	19.880400274024716
12-13	24.5641352934653	24.641101975551813	28.731602775925776	22.063159955057113
14-15	22.218079908219575	25.934873324171637	26.255654120536477	25.591392647072308
16-17	21.99044354334952	28.635005413160393	27.07764342113504	22.296907622355043
18-19	21.757581407720245	28.586796572325117	28.174541545502365	21.48108047445227
20-21	23.074513390561684	27.830541780507566	27.419659667487924	21.675285161442826
22-23	22.95936383563066	27.64700587112248	27.017048953265732	22.376581339981126
24-25	22.55561397385315	27.74478984876534	27.50471641843799	22.19487975894352
26-27	22.47318705927328	28.013830428466925	27.088539727356803	22.424442784902993
28-29	22.285746097357546	28.227321584454003	27.20794619502371	22.278986123164742
30-31	22.74564427600483	27.81470955028317	27.1654794030286	22.274166770683408
32-33	22.69835527569388	28.063484734424442	26.756221911578464	22.481938078303216
34-35	22.799395191578633	28.013249846677002	27.14397029687221	22.043384664872153
36-37	22.708751393995836	27.828426523232157	27.051644266071897	22.41117781670011
38-39	22.599217821526235	27.89131735938209	27.300673408782046	22.208791410309633
40-41	22.701162567444886	27.49923057429316	27.505914028028084	22.29369283023387
42-43	22.54047323916276	27.587724240636526	27.246383536279534	22.62541898392118
44-45	22.6584332023695	27.90683029301472	27.243294207695335	22.19144229692044
46-47	22.534598472984896	27.720684794658606	26.995238822325014	22.749477910031484
48-49	22.610200936646642	27.56181297737756	27.31004002646334	22.51794605951246
50-51	22.62178634034304	27.68658931071949	27.03394131946462	22.65768302947285
52-53	22.48698366225193	27.777088483475808	27.005924677871533	22.73000317640072
54-55	22.59756265048087	27.57854229938471	27.18373839258062	22.640156657553796
56-57	22.775696824649682	27.545727563826333	27.001306028281814	22.677269583242175
58-59	22.509130054649397	27.59434923838776	27.367646111535333	22.52887459542751
60-61	22.580581005070556	27.831324915150997	27.05450501072373	22.53358906905472
62-63	22.598386787590385	27.5057632485663	27.01115968217473	22.884690281668586
64-65	22.366273360984167	27.613656414776237	27.166288740974338	22.853781483265255
66-67	22.561408343232394	27.599792321348133	26.949520364628732	22.88927897079074
68-69	22.462856224182502	27.64027289688027	27.08951797757092	22.807352901366308
70-71	22.529592603310448	27.354748490080848	27.106591491415937	23.009067415192767
72-73	22.695768216477664	27.46824206698037	27.214746517709443	22.621243198832527
74-75	22.67285199788519	27.74935376105816	27.006020145970723	22.571774095085935
76-77	22.632290048653914	27.55360117846022	26.90637397858715	22.90773479429872
78-79	22.62335637624641	27.619630064841687	26.90994590909568	22.847067649816225
80-81	22.538767383983483	27.685413227236168	27.091318309285818	22.68450107949453
82-83	22.87567053688363	27.36169474112572	27.415968603366238	22.34666611862441
84-85	23.009323188777614	27.51863212461796	26.84098782357206	22.631056863032367
86-87	22.414233335708797	27.780615443938235	27.02777027389095	22.777380946462024
88-89	22.904394225412894	27.302740681636035	26.99734154035982	22.795523552591252
90-91	22.82922104761666	27.328625360144258	27.112171436898947	22.729982155340135
92-93	23.018998425544844	27.336364389691465	27.1081291605668	22.536508024196888
94-95	22.493738406605136	27.484406234765885	27.231135996902683	22.790719361726293
96-97	22.608548080175	27.41958739570456	27.091728722525648	22.88013580159479
98-99	22.548749463323357	27.580748980728696	26.944161543701885	22.92634001224606
100	22.870080500894456	27.30688800582633	26.807381193124368	23.01565030015485
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1503.0
1	1059.0
2	673.5
3	700.5
4	650.5
5	607.0
6	533.0
7	483.5
8	540.5
9	577.5
10	497.5
11	435.5
12	431.5
13	454.5
14	508.5
15	614.5
16	739.5
17	902.0
18	1129.5
19	1488.5
20	2146.5
21	3233.5
22	4870.5
23	7097.5
24	10540.0
25	15034.5
26	21053.0
27	28299.5
28	36138.0
29	45941.5
30	55937.5
31	65856.0
32	77974.5
33	88485.0
34	97741.0
35	106637.0
36	117431.5
37	131678.0
38	137997.5
39	138343.5
40	141603.0
41	145112.5
42	146883.5
43	151605.0
44	159681.5
45	172041.5
46	183469.0
47	189291.0
48	189530.5
49	185681.0
50	178115.0
51	171679.0
52	167153.5
53	161052.0
54	153721.0
55	139253.5
56	124042.5
57	108668.0
58	93793.5
59	82736.5
60	68562.5
61	53566.5
62	41496.5
63	31376.5
64	22758.5
65	15195.5
66	10717.0
67	8018.0
68	5874.5
69	4294.0
70	3289.5
71	2672.0
72	2017.5
73	1594.0
74	1267.0
75	865.0
76	622.0
77	420.5
78	279.0
79	175.0
80	101.5
81	75.5
82	68.5
83	52.5
84	37.5
85	29.5
86	24.5
87	19.5
88	17.5
89	17.0
90	15.5
91	12.0
92	9.0
93	8.5
94	8.0
95	7.0
96	7.5
97	6.5
98	5.5
99	8.5
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06010690679386976
2	0.009564972672453365
3	0.011796063295820084
4	0.009940502777376476
5	0.011862333314335928
6	0.01290056360441747
7	0.0030263308455568383
8	0.0060305716849417285
9	0.007488512092290278
10-11	0.007400152067602488
12-13	0.006627001851584317
14-15	0.010570067953276986
16-17	0.010282897873041666
18-19	0.008107032265104815
20-21	0.007322837046000671
22-23	0.00257348571903191
24-25	0.004285461197357858
26-27	0.00548936653372901
28-29	0.0063950567867788665
30-31	0.009255712586046096
32-33	0.010857238033512306
34-35	0.011972783345195666
36-37	0.018257390101114792
38-39	0.0189311352893592
40-41	0.018356795128888558
42-43	0.02438736681383029
44-45	0.02776713775813829
46-47	0.030329578474084226
48-49	0.03154452881354135
50-51	0.03196423893080836
52-53	0.030296443464826305
54-55	0.03643746518062777
56-57	0.03896677088731579
58-59	0.03606193507570466
60-61	0.03732106542750568
62-63	0.03294724420546003
64-65	0.034758624711559744
66-67	0.036967625328754514
68-69	0.03935334599532487
70-71	0.03919871595212124
72-73	0.03552072992449194
74-75	0.03780704556328853
76-77	0.037707640535514765
78-79	0.0371443453781301
80-81	0.041959966723614704
82-83	0.03833720571141527
84-85	0.03426159957269092
86-87	0.033168144267179504
88-89	0.030506298523459804
90-91	0.032925154199288084
92-93	0.034438319622066504
94-95	0.038171530665125665
96-97	0.03332277431038314
98-99	0.03085973862221097
100	0.02805430783837361
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4526934.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.40939785149009
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.04223103661117	36.34094588137373
2	15.593259523371339	16.65653203370553
3	6.676082626093289	10.696966591993117
4	3.3519082114711183	7.160935969125501
5	1.9380635064647866	5.175540243911587
6	1.1943633075620221	3.8274135043680997
7	0.8081588904481036	3.021429579501309
8	0.5348361027155486	2.2852219356220127
9	0.3957355015578468	1.9022395361995812
>10	1.4422053685095002	11.799087046060023
>50	0.01903283966044986	0.6653594759558115
>100	0.00397085968535925	0.36178073914756653
>500	7.61129247610569E-5	0.033374844392473346
>1k	7.611292476105687E-5	0.07317261864366871
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	2.2090006171947726E-5
5	0.0	0.0	0.0	0.0	2.2090006171947726E-5
6	0.0	0.0	0.0	0.0	4.418001234389545E-5
7	0.0	0.0	0.0	0.0	4.418001234389545E-5
8	0.0	0.0	0.0	2.2090006171947726E-5	4.418001234389545E-5
9	0.0	0.0	0.0	2.2090006171947726E-5	4.418001234389545E-5
10-11	0.0	0.0	0.0	2.2090006171947726E-5	4.418001234389545E-5
12-13	0.0	0.0	0.0	3.313500925792159E-5	4.418001234389545E-5
14-15	0.0	0.0	0.0	4.418001234389545E-5	5.522501542986931E-5
16-17	0.0	0.0	0.0	5.522501542986931E-5	6.627001851584317E-5
18-19	0.0	0.0	0.0	8.83600246877909E-5	8.83600246877909E-5
20-21	0.0	0.0	0.0	1.3254003703168637E-4	8.83600246877909E-5
22-23	0.0	0.0	0.0	3.2030508949324203E-4	8.83600246877909E-5
24-25	0.0	0.0	0.0	7.731502160181704E-4	1.3254003703168634E-4
26-27	0.0	0.0	0.0	0.0014137603950046544	1.3254003703168634E-4
28-29	0.0	0.0	0.0	0.004296506200443833	1.3254003703168634E-4
30-31	0.0	0.0	0.0	0.013629533808091746	1.3254003703168634E-4
32-33	0.0	0.0	0.0	0.03578580999855532	1.5463004320363406E-4
34-35	0.0	0.0	0.0	0.0695503844323774	1.767200493755818E-4
36-37	0.0	0.0	0.0	0.1211084588377034	1.767200493755818E-4
38-39	0.0	0.0	0.0	0.19092392334414418	1.767200493755818E-4
40-41	0.0	0.0	0.0	0.26392034873934545	1.9881005554752952E-4
42-43	0.0	0.0	0.0	0.3364418390018498	2.0985505863350338E-4
44-45	0.0	0.0	0.0	0.4272869893839848	2.2090006171947724E-4
46-47	0.0	0.0	0.0	0.5337829091389448	2.2090006171947724E-4
48-49	0.0	0.0	0.0	0.6472924058534982	2.2090006171947724E-4
50-51	0.0	0.0	0.0	0.7580075167873002	2.2090006171947724E-4
52-53	0.0	0.0	0.0	0.8666351221378532	2.319450648054511E-4
54-55	0.0	0.0	0.0	0.9867716207039909	2.4299006789142496E-4
56-57	0.0	0.0	0.0	1.1280813901859403	2.4299006789142496E-4
58-59	0.0	0.0	0.0	1.2820597782075018	2.4299006789142496E-4
60-61	0.0	0.0	0.0	1.4338954356303848	2.4299006789142496E-4
62-63	0.0	0.0	0.0	1.5838865775379096	2.650800740633727E-4
64-65	0.0	0.0	0.0	1.7426364068926121	2.650800740633727E-4
66-67	0.0	0.0	0.0	1.9220514370211714	2.650800740633727E-4
68-69	0.0	0.0	0.0	2.117923521747832	2.650800740633727E-4
70-71	0.0	0.0	0.0	2.3196936381224025	2.871700802353204E-4
72-73	0.0	0.0	0.0	2.5229769199197514	3.9762011109505905E-4
74-75	0.0	0.0	0.0	2.729971322753988	3.9762011109505905E-4
76-77	0.0	1.1045003085973863E-5	0.0	2.953455915195583	3.9762011109505905E-4
78-79	0.0	2.2090006171947726E-5	0.0	3.19959380896651	3.9762011109505905E-4
80-81	0.0	2.2090006171947726E-5	0.0	3.459968711715258	4.086651141810329E-4
82-83	0.0	2.2090006171947726E-5	0.0	3.7234914403435084	4.859801357828499E-4
84-85	0.0	2.2090006171947726E-5	0.0	3.988847639484031	4.859801357828499E-4
86-87	0.0	2.2090006171947726E-5	0.0	4.2717653935312505	4.859801357828499E-4
88	0.0	2.2090006171947726E-5	0.0	4.4878277438990715	4.859801357828499E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4460	0.0	35.31782	1
GTATCAA	6790	0.0	32.062336	1
CAACGCA	6640	0.0	30.29176	5
ATCAACG	6730	0.0	29.81684	3
AACGCAG	7715	0.0	27.045252	6
TATCAAC	8165	0.0	26.015434	2
TCAACGC	7915	0.0	25.76841	4
GTACATG	13315	0.0	24.119232	1
ACGCAGA	8270	0.0	24.035862	7
TACATGG	13135	0.0	24.007185	2
CGCAGAG	8390	0.0	23.636856	8
ACATGGG	13115	0.0	23.542137	3
GTACTAG	1925	0.0	21.739182	1
CATGGGG	10380	0.0	21.369455	4
GCAGAGT	10060	0.0	19.152485	9
ATGGGGG	7200	0.0	18.928417	5
GCATCGT	1905	0.0	18.501438	8
TATTCGC	390	1.8189894E-11	18.074484	9
TAGGCAT	2835	0.0	18.068506	5
GTATAAG	2435	0.0	17.958412	1
>>END_MODULE