FastQCFastQC Report
Mon 16 Jan 2017
ERR1146537_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146537_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4526934
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138520.30599076549381987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130400.2880536804821983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71080.15701576387020444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81600.056.643261
GTATCAA153150.042.8115581
ATCAACG188100.034.3207933
CAACGCA188400.034.216235
TATCAAC198400.032.9739722
AACGCAG203950.032.1699376
TCAACGC202000.032.0987784
ACGCAGA218500.029.5532027
CGCAGAG222000.029.0872768
GCAGAGT247900.025.8018539
GTGGTAT28100.025.6278421
GTACTAG19800.024.247221
TACATGG126400.024.0537932
GTACATG129300.023.9890941
ACATGGG125450.023.0879633
AGAGTAC228200.022.92180410-11
GAGTACT182100.022.72420512-13
CAGAGTA240400.022.38413210-11
TGGTATC33050.021.3608192
TAGTACT27600.021.1245844
Produced by FastQC (version 0.11.5)