##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146537_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4526934
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5888418077224	34.0	31.0	34.0	31.0	34.0
2	32.65723887293254	34.0	31.0	34.0	31.0	34.0
3	32.753804893113085	34.0	31.0	34.0	31.0	34.0
4	36.22515039980702	37.0	37.0	37.0	35.0	37.0
5	36.24878339291008	37.0	37.0	37.0	35.0	37.0
6	36.31659904915777	37.0	37.0	37.0	35.0	37.0
7	36.30485776907726	37.0	37.0	37.0	35.0	37.0
8	36.31522902697499	37.0	37.0	37.0	35.0	37.0
9	38.13983504066991	39.0	39.0	39.0	37.0	39.0
10-11	38.10325630989982	39.0	38.5	39.0	37.0	39.0
12-13	38.08012895703803	39.0	38.0	39.0	37.0	39.0
14-15	39.55672492684894	41.0	39.0	41.0	37.0	41.0
16-17	39.55506806593602	41.0	39.0	41.0	37.0	41.0
18-19	39.602223049861124	41.0	39.5	41.0	37.0	41.0
20-21	39.63148048988565	41.0	40.0	41.0	37.0	41.0
22-23	39.59242580077377	41.0	40.0	41.0	37.0	41.0
24-25	39.578411017258034	41.0	40.0	41.0	37.0	41.0
26-27	39.50809974256307	41.0	39.0	41.0	36.5	41.0
28-29	39.42400054871575	41.0	39.0	41.0	36.0	41.0
30-31	39.32279363913854	41.0	39.0	41.0	36.0	41.0
32-33	39.20203353969817	41.0	39.0	41.0	35.0	41.0
34-35	39.12452478874222	41.0	39.0	41.0	35.0	41.0
36-37	39.04471381734304	41.0	39.0	41.0	35.0	41.0
38-39	38.942517496389385	41.0	39.0	41.0	35.0	41.0
40-41	38.85374173778544	41.0	39.0	41.0	35.0	41.0
42-43	38.75888206013165	41.0	39.0	41.0	35.0	41.0
44-45	38.60639684607728	40.0	38.0	41.0	35.0	41.0
46-47	38.37279558305909	40.0	38.0	41.0	34.5	41.0
48-49	38.162669369599826	40.0	38.0	41.0	34.0	41.0
50-51	38.01585996173127	40.0	38.0	41.0	34.0	41.0
52-53	37.89755229477611	40.0	37.5	41.0	33.5	41.0
54-55	37.763778972699846	40.0	37.0	41.0	33.0	41.0
56-57	37.57775759487547	40.0	37.0	41.0	33.0	41.0
58-59	37.380205454729406	40.0	36.0	41.0	33.0	41.0
60-61	37.207391249795116	39.0	36.0	41.0	33.0	41.0
62-63	36.96324399693037	39.0	35.0	41.0	32.5	41.0
64-65	36.679791222933666	39.0	35.0	41.0	32.0	41.0
66-67	36.42557114815458	38.0	35.0	41.0	32.0	41.0
68-69	36.11590924453505	37.0	35.0	40.0	32.0	41.0
70-71	35.831274213408015	37.0	35.0	39.5	31.5	41.0
72-73	35.499466195000856	36.0	35.0	39.0	31.0	41.0
74-75	35.16685266451863	36.0	35.0	39.0	31.0	41.0
76-77	34.855873423381034	35.0	35.0	38.0	31.0	40.0
78-79	34.55865415753797	35.0	35.0	37.0	31.0	39.0
80-81	34.280817657160455	35.0	35.0	37.0	31.0	39.0
82-83	34.017032499258875	35.0	35.0	36.0	30.0	38.0
84-85	33.798990221637865	35.0	34.5	36.0	30.0	37.0
86-87	33.60548298252195	35.0	34.0	36.0	30.0	37.0
88-89	33.44589075078187	35.0	34.0	35.0	30.0	36.5
90-91	33.35341878189521	35.0	34.0	35.0	30.0	36.0
92-93	33.260271742419924	35.0	34.0	35.0	30.0	36.0
94-95	33.1820404273621	35.0	34.0	35.0	30.0	36.0
96-97	33.12863949419187	35.0	34.0	35.0	30.0	36.0
98-99	33.078378765849024	35.0	34.0	35.0	30.0	35.0
100	32.55042574068895	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.28844022486753573
1101	2	0.2521757039683976
1101	3	0.27193019887754843
1101	4	0.2038511720364582
1101	5	0.17711642531381955
1101	6	0.18782404279985343
1101	7	0.17754458423219432
1101	8	0.1535381854362612
1101	9	0.20996022549385174
1101	10-11	0.2360856073513915
1101	12-13	0.21582446286515733
1101	14-15	0.3296182308836819
1101	16-17	0.337816110774817
1101	18-19	0.31344556936622325
1101	20-21	0.2970292466629161
1101	22-23	0.3463629286270944
1101	24-25	0.332335916890969
1101	26-27	0.2956122371789789
1101	28-29	0.3646277674481553
1101	30-31	0.3095053906827516
1101	32-33	0.33722263715345235
1101	34-35	0.32229064436813815
1101	36-37	0.3500752393835498
1101	38-39	0.4345649217717451
1101	40-41	0.362546373863708
1101	42-43	0.35670121925763
1101	44-45	0.37500129039184316
1101	46-47	0.3924321668496873
1101	48-49	0.3672189969076953
1101	50-51	0.3494733059997941
1101	52-53	0.3178109062542731
1101	54-55	0.27966464120970613
1101	56-57	0.3071263232127137
1101	58-59	0.3117899327988738
1101	60-61	0.247347253783623
1101	62-63	0.26899109367275287
1101	64-65	0.27409218289363224
1101	66-67	0.3119235072081281
1101	68-69	0.2986485541053554
1101	70-71	0.262847977410523
1101	72-73	0.24621157682781103
1101	74-75	0.24427308586415108
1101	76-77	0.2498102157467983
1101	78-79	0.2383679704212085
1101	80-81	0.23864872522089087
1101	82-83	0.17103187476288184
1101	84-85	0.18770476511551237
1101	86-87	0.17899233009628546
1101	88-89	0.19186561481588882
1101	90-91	0.20591667111509793
1101	92-93	0.20894810708192324
1101	94-95	0.14788257063662513
1101	96-97	0.1281101455043725
1101	98-99	0.12181270829737656
1101	100	0.2029815183243855
1102	1	0.2642132694302646
1102	2	0.2508472957880272
1102	3	0.22497945371423356
1102	4	0.16366085002622555
1102	5	0.12308970716629375
1102	6	0.14468367710754393
1102	7	0.16450479530683992
1102	8	0.16079303029627567
1102	9	0.18741002610860846
1102	10-11	0.1977037733806739
1102	12-13	0.22890571621718436
1102	14-15	0.30579474386837546
1102	16-17	0.2923280444177294
1102	18-19	0.3015754507738748
1102	20-21	0.29855168559991796
1102	22-23	0.2780761535323677
1102	24-25	0.25089260468571695
1102	26-27	0.2847895403513405
1102	28-29	0.2976820937256335
1102	30-31	0.3271614016823534
1102	32-33	0.3175279828255455
1102	34-35	0.33329801984208984
1102	36-37	0.3313918968780456
1102	38-39	0.36732440125166477
1102	40-41	0.3867307122741295
1102	42-43	0.36102724870466574
1102	44-45	0.3949224817193837
1102	46-47	0.3790640765418658
1102	48-49	0.370907390428151
1102	50-51	0.35352271140399694
1102	52-53	0.3878914336140795
1102	54-55	0.3440150094873431
1102	56-57	0.34665255785176186
1102	58-59	0.34302815512173623
1102	60-61	0.34545561804262803
1102	62-63	0.31756184027170065
1102	64-65	0.3819696944677773
1102	66-67	0.35883334236004316
1102	68-69	0.2807148831817585
1102	70-71	0.24515193179207273
1102	72-73	0.25246705897358623
1102	74-75	0.22980629037861178
1102	76-77	0.21692752476678123
1102	78-79	0.21437878618785078
1102	80-81	0.23762989961250724
1102	82-83	0.2365794006992985
1102	84-85	0.2259240970509353
1102	86-87	0.26441487040373346
1102	88-89	0.2778323574860835
1102	90-91	0.2332744387940906
1102	92-93	0.2610551185676613
1102	94-95	0.26334512513710706
1102	96-97	0.19658257676709212
1102	98-99	0.23349533239739628
1102	100	0.1329470512020876
1103	1	0.2101282816146437
1103	2	0.17267739427035167
1103	3	0.20196421765076167
1103	4	0.12155592611221522
1103	5	0.11007059361612193
1103	6	0.09053066764222706
1103	7	0.1185746572186801
1103	8	0.12084632692917552
1103	9	0.16063074763106044
1103	10-11	0.13586478070971708
1103	12-13	0.15858455296668694
1103	14-15	0.2064633436262966
1103	16-17	0.21077307624332775
1103	18-19	0.21971862424688027
1103	20-21	0.23580735649438367
1103	22-23	0.23341276777566833
1103	24-25	0.20768788046284214
1103	26-27	0.19018070385899222
1103	28-29	0.22592930354662144
1103	30-31	0.22165374513402014
1103	32-33	0.1977974849946591
1103	34-35	0.20409033478249228
1103	36-37	0.20579244731720792
1103	38-39	0.2084877432701404
1103	40-41	0.2241844179689565
1103	42-43	0.1829847354824139
1103	44-45	0.18939025259574294
1103	46-47	0.15449636914489417
1103	48-49	0.15514708300061386
1103	50-51	0.23069159981177023
1103	52-53	0.19050137911478515
1103	54-55	0.19722723714668433
1103	56-57	0.21102037817084351
1103	58-59	0.18242046379617705
1103	60-61	0.1737198739823569
1103	62-63	0.15654665069763496
1103	64-65	0.17054810431194056
1103	66-67	0.21745409125959725
1103	68-69	0.20256176950828575
1103	70-71	0.15273517226822975
1103	72-73	0.15472706494983868
1103	74-75	0.23849436470956675
1103	76-77	0.18145624169374486
1103	78-79	0.1342607788723882
1103	80-81	0.16676777755937877
1103	82-83	0.17273646440091284
1103	84-85	0.11969119440347953
1103	86-87	0.14321293767931564
1103	88-89	0.1304738966989447
1103	90-91	0.10182011627231446
1103	92-93	0.061477436720778655
1103	94-95	0.10662967303260018
1103	96-97	0.05725083908372852
1103	98-99	0.12663690007661188
1103	100	0.1507677970023451
1104	1	0.11689813567808471
1104	2	0.12405937255144295
1104	3	0.10327569755682475
1104	4	0.07959281466639112
1104	5	0.06109792638586953
1104	6	0.04516140276534486
1104	7	0.08810579062628676
1104	8	0.08937487224306295
1104	9	0.14478248105593394
1104	10-11	0.12259360485038684
1104	12-13	0.12062727193062983
1104	14-15	0.19474361775989735
1104	16-17	0.18107726440688765
1104	18-19	0.1371102388489973
1104	20-21	0.13660327870702815
1104	22-23	0.12912017768772444
1104	24-25	0.1592332264738161
1104	26-27	0.15350085783337875
1104	28-29	0.1896736500503451
1104	30-31	0.1586402286985802
1104	32-33	0.11160289949636137
1104	34-35	0.1155789600543855
1104	36-37	0.17766736756222912
1104	38-39	0.22027217215037354
1104	40-41	0.22111558703165457
1104	42-43	0.1928559705808297
1104	44-45	0.17177858065510776
1104	46-47	0.1889369300070456
1104	48-49	0.16508268565551276
1104	50-51	0.14170258748919196
1104	52-53	0.14767834287741977
1104	54-55	0.11781581397584517
1104	56-57	0.15480117466522358
1104	58-59	0.10488575006568368
1104	60-61	0.10591122282711041
1104	62-63	0.16539900789337736
1104	64-65	0.13694917910655846
1104	66-67	0.09786805421828859
1104	68-69	0.13348857832316696
1104	70-71	0.13796679657875188
1104	72-73	0.0892003288782135
1104	74-75	0.05733773406360143
1104	76-77	0.09009857843490465
1104	78-79	0.13215318084658634
1104	80-81	0.10667011009095262
1104	82-83	0.09485386797926765
1104	84-85	0.10569010360352138
1104	86-87	0.10164145256319301
1104	88-89	0.08723155219127676
1104	90-91	0.08893998975604944
1104	92-93	0.0774304867540252
1104	94-95	0.09047497287659212
1104	96-97	0.03868110094898469
1104	98-99	0.04684946947032387
1104	100	0.0903831927342722
1105	1	0.1853531733718512
1105	2	0.17715307843098316
1105	3	0.16340782159488754
1105	4	0.10709351166636338
1105	5	0.08748184800118253
1105	6	0.08574399346768047
1105	7	0.09916436510635407
1105	8	0.09482153725978293
1105	9	0.12784084918050098
1105	10-11	0.16827450437033775
1105	12-13	0.16608244823667917
1105	14-15	0.21742896955239388
1105	16-17	0.19547940072150283
1105	18-19	0.2072651712719491
1105	20-21	0.20202312501018582
1105	22-23	0.2092914292501078
1105	24-25	0.22331565054382452
1105	26-27	0.21835920310680024
1105	28-29	0.27699961355138214
1105	30-31	0.2698065296543817
1105	32-33	0.19155750528644688
1105	34-35	0.1752141504297171
1105	36-37	0.18249346566196323
1105	38-39	0.21393546773530403
1105	40-41	0.20123947835531908
1105	42-43	0.13134688867553024
1105	44-45	0.1604574730601911
1105	46-47	0.13599839086347743
1105	48-49	0.19170844731975478
1105	50-51	0.1393584819834075
1105	52-53	0.14957272741950334
1105	54-55	0.14308529833540007
1105	56-57	0.1914442307312214
1105	58-59	0.15054419354552095
1105	60-61	0.1133228788772982
1105	62-63	0.11641158150035835
1105	64-65	0.18505305534667826
1105	66-67	0.10381562896720453
1105	68-69	0.11598888384284578
1105	70-71	0.08752061531714617
1105	72-73	0.08813986864091561
1105	74-75	0.12335061487821974
1105	76-77	0.07641315989945241
1105	78-79	0.10753008284879684
1105	80-81	0.12290285129251544
1105	82-83	0.12694303666341966
1105	84-85	0.1257445852577206
1105	86-87	0.09344493540498178
1105	88-89	0.13639628877630372
1105	90-91	0.1724377735724545
1105	92-93	0.11962267289729311
1105	94-95	0.11184068221825783
1105	96-97	0.09669850618055165
1105	98-99	0.07549891295438016
1105	100	0.09954668248392551
1106	1	0.09976547659581314
1106	2	0.10245733894954867
1106	3	0.12381633376477197
1106	4	0.11669035021523655
1106	5	0.07628521322158832
1106	6	0.0874002028032308
1106	7	0.06645639731943476
1106	8	0.06784714983719198
1106	9	0.13111016964108302
1106	10-11	0.1110207975621691
1106	12-13	0.06376102444559706
1106	14-15	0.13335637927144717
1106	16-17	0.1322632492034188
1106	18-19	0.15049280957270383
1106	20-21	0.13645690632664298
1106	22-23	0.16235586619055908
1106	24-25	0.1383502965995973
1106	26-27	0.11612592625197493
1106	28-29	0.14293337938050854
1106	30-31	0.18117366983138794
1106	32-33	0.1533109035394702
1106	34-35	0.12483696750535245
1106	36-37	0.16241062225833502
1106	38-39	0.1970647491454116
1106	40-41	0.1638517807290043
1106	42-43	0.21040305748707766
1106	44-45	0.13982047309929868
1106	46-47	0.14066723519278668
1106	48-49	0.14889925176409236
1106	50-51	0.1404735459829709
1106	52-53	0.17344704537571687
1106	54-55	0.18539350347317196
1106	56-57	0.17789479572890343
1106	58-59	0.1827402036463397
1106	60-61	0.17444199294067175
1106	62-63	0.16963580418033786
1106	64-65	0.22255785396450278
1106	66-67	0.12469300948927042
1106	68-69	0.1576933402819094
1106	70-71	0.08975413043850011
1106	72-73	0.05332014693716047
1106	74-75	0.06441111397371913
1106	76-77	0.07981370214735506
1106	78-79	0.07345392791370386
1106	80-81	0.10028297157600008
1106	82-83	0.1181645969251548
1106	84-85	0.09340334937110129
1106	86-87	0.08036059900459946
1106	88-89	0.10033058495869085
1106	90-91	0.1003581421885329
1106	92-93	0.08764468835088479
1106	94-95	0.06938803383673076
1106	96-97	0.06902597446646297
1106	98-99	0.06570169692592032
1106	100	0.11435515754029524
1107	1	0.094086027131425
1107	2	0.12434347390009748
1107	3	0.1266811363740601
1107	4	0.06560958642586456
1107	5	0.027255950934488737
1107	6	0.03017072344890437
1107	7	0.01794348151172187
1107	8	0.06200891150627541
1107	9	0.05079918406558903
1107	10-11	0.08940441373505337
1107	12-13	0.10422532110136729
1107	14-15	0.12668265642735577
1107	16-17	0.10145266889908555
1107	18-19	0.10970342764989027
1107	20-21	0.11365822157424077
1107	22-23	0.11786398106595897
1107	24-25	0.10229737716836951
1107	26-27	0.1239285606004259
1107	28-29	0.14459254439258018
1107	30-31	0.12843566313956245
1107	32-33	0.14380876249027352
1107	34-35	0.17090012070038796
1107	36-37	0.16404294557509047
1107	38-39	0.18778351502260193
1107	40-41	0.1845332052175479
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2216	26-27	-0.3237307310859805
2216	28-29	-0.3586809202521408
2216	30-31	-0.35754629234097735
2216	32-33	-0.33333771404108603
2216	34-35	-0.33714403989520036
2216	36-37	-0.364593253220562
2216	38-39	-0.36353289232010866
2216	40-41	-0.33569895286450446
2216	42-43	-0.361617425235103
2216	44-45	-0.34170696402890144
2216	46-47	-0.3467183785906798
2216	48-49	-0.31152649501862584
2216	50-51	-0.33437473391374795
2216	52-53	-0.36276286716655193
2216	54-55	-0.36239825407380977
2216	56-57	-0.3290781735006192
2216	58-59	-0.3244512915894475
2216	60-61	-0.3114366512396032
2216	62-63	-0.33788459652377156
2216	64-65	-0.2557024077428025
2216	66-67	-0.26410963063282367
2216	68-69	-0.27358504651958526
2216	70-71	-0.3125302345173324
2216	72-73	-0.3087505671053137
2216	74-75	-0.26355892840039985
2216	76-77	-0.222802757683354
2216	78-79	-0.2442591895988997
2216	80-81	-0.26221642936879874
2216	82-83	-0.20692534800575402
2216	84-85	-0.24578082309388094
2216	86-87	-0.16641520091155826
2216	88-89	-0.2121102802109931
2216	90-91	-0.219960307225314
2216	92-93	-0.23827066966482846
2216	94-95	-0.1740638012251594
2216	96-97	-0.20984267070762286
2216	98-99	-0.277919000049728
2216	100	-0.3768338152315067
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	22.0
8	108.0
9	180.0
10	293.0
11	331.0
12	278.0
13	291.0
14	401.0
15	834.0
16	1496.0
17	2974.0
18	5455.0
19	8662.0
20	12704.0
21	18894.0
22	27972.0
23	33552.0
24	32560.0
25	27039.0
26	22943.0
27	23919.0
28	28131.0
29	33971.0
30	40848.0
31	52091.0
32	67955.0
33	91263.0
34	134309.0
35	217439.0
36	395415.0
37	995733.0
38	1588429.0
39	658187.0
40	2254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.285653692449415	19.993726072366663	13.61546125518317	24.105158980000752
2	15.130118149322666	21.07375830885985	40.11144518772684	23.68467835409065
3	17.435232920263118	26.339775838463027	31.404639010151286	24.82035223112257
4	11.981685617682961	18.187541501599096	39.75675368803698	30.07401919268096
5	12.574146844452617	38.21042521980525	35.88053148387388	13.33489645186825
6	28.929638436680687	39.27561857751692	18.35722282752289	13.437520158279497
7	25.12948057117687	33.79267292167281	23.451103992238455	17.626742514911857
8	25.200477851013513	34.85937722971	21.23686804358093	18.70327687569556
9	26.07566180553991	14.731493765979359	21.25575499885795	37.93708942962279
10-11	23.810784517733193	26.251133769566774	29.56398304017686	20.374098672523168
12-13	25.066104343469554	24.44025028860593	28.99096828007654	21.502677087847978
14-15	22.349145331666715	25.358799884645922	27.288804789734506	25.00324999395286
16-17	21.24896232196007	28.188692390920657	28.048663841796678	22.513681445322593
18-19	21.002846518195316	27.184602205377857	30.595796183465453	21.216755092961375
20-21	22.17772558645653	26.36035780508397	30.279080719975155	21.182835888484348
22-23	22.058096505435486	26.33519155204117	29.85065329244015	21.75605865008319
24-25	21.728293365885165	26.428439204105914	30.30325823173035	21.54000919827857
26-27	21.7029892638152	26.67544965312063	29.898602453669525	21.72295862939464
28-29	21.47227493757915	26.833653159876675	30.063974881006633	21.63009702153754
30-31	21.94555664766297	26.423617972065145	30.025422331337236	21.605403048934647
32-33	21.892744820956032	26.64959666204903	29.62188489167631	21.835773625318627
34-35	21.966539142982082	26.702734990379117	29.817303186096623	21.51342268054218
36-37	21.9724317135473	26.577300228003487	29.657882113483947	21.79238594496526
38-39	21.906793252076916	26.610184355577232	29.846303345509423	21.636719046836426
40-41	22.031936941693335	26.362821630367023	29.861097918247047	21.744143509692595
42-43	22.052925784835335	26.431877850319367	29.4334539823566	22.0817423824887
44-45	22.162299120835765	26.862687663756734	29.205023379066485	21.76998983634101
46-47	22.241854151468242	26.730908249478535	28.635579690175835	22.391657908877388
48-49	22.473474417998972	26.749068099198492	28.59270139069675	22.184756092105783
50-51	22.39902564765835	26.991788759846823	28.184734182044263	22.424451410450562
52-53	22.177189990194133	27.2092197696383	28.115307205999006	22.49828303416856
54-55	22.31795328329129	27.047644504137523	28.15721051678205	22.477191695789134
56-57	22.579627585055157	26.956344825118194	27.91300686608852	22.551020723738127
58-59	22.142287045155367	27.113742554607644	28.350081393162885	22.3938890070741
60-61	22.304980366570938	27.353816250190118	28.006411970543283	22.33479141269566
62-63	22.312522345690887	27.001027970813006	27.942161924124555	22.74428775937155
64-65	22.029342486407078	27.078348801696595	28.12693538633169	22.765373325564635
66-67	22.198346682047944	27.042178815964906	27.935486557527195	22.82398794445995
68-69	22.181485372343804	26.896280234574448	28.098433964937986	22.823800428143766
70-71	22.228206267843525	26.678517433306855	28.01689783540527	23.076378463444353
72-73	22.379980106910168	26.686662848577797	28.12990044066342	22.803456603848613
74-75	22.36407408495589	26.99954205545746	27.826404777468333	22.809979082118314
76-77	22.311363722467757	26.85541901322149	27.649795035544734	23.183422228766023
78-79	22.20759485953314	26.914233215460282	27.760948763216536	23.117223161790047
80-81	22.110252827991765	27.000530049990818	27.852740868731946	23.036476253285475
82-83	22.411458986255024	26.671456523215802	28.1629317014686	22.754152789060573
84-85	22.54424079895205	26.798879152096454	27.602945042225084	23.053935006726412
86-87	21.925601301013003	27.04848358734632	27.851079781591693	23.17483533004899
88-89	22.32916994887922	26.67228269249376	27.79365070248553	23.204896656141496
90-91	22.24175346934592	26.650510036152504	27.863715265122046	23.244021229379534
92-93	22.395720812364395	26.67538338310212	27.791867520047788	23.1370282844857
94-95	21.85051356520116	26.751735308622337	28.021944219402435	23.37580690677407
96-97	21.9855873388599	26.63046473625294	27.88703583946931	23.496912085417854
98-99	21.8797172773169	26.750457458891102	27.750753577026444	23.61907168676555
100	22.130611763422053	26.53431257658392	27.620818410491776	23.714257249502253
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	475.0
1	310.5
2	350.0
3	527.5
4	330.0
5	264.5
6	590.5
7	1503.5
8	2887.0
9	3488.0
10	2530.0
11	1555.0
12	1609.0
13	1978.5
14	2429.0
15	2980.5
16	3578.5
17	4346.0
18	5203.5
19	6102.0
20	7147.5
21	8572.5
22	10402.0
23	12753.5
24	16170.0
25	20417.0
26	26068.5
27	32773.0
28	39927.5
29	49279.0
30	58885.5
31	67576.0
32	78471.0
33	88571.5
34	96872.5
35	104474.0
36	114136.0
37	127808.5
38	134518.5
39	135040.5
40	138080.0
41	141731.0
42	143784.5
43	147835.0
44	156398.0
45	169559.0
46	180659.0
47	186221.0
48	186451.5
49	182705.5
50	175274.0
51	167820.0
52	162665.0
53	157379.5
54	151696.0
55	137816.0
56	122335.0
57	107680.5
58	92796.0
59	81696.5
60	67586.0
61	52124.0
62	39849.0
63	30135.5
64	21704.5
65	14311.5
66	9908.0
67	7418.5
68	5516.5
69	3875.5
70	2891.0
71	2318.5
72	1750.0
73	1375.5
74	1079.0
75	790.0
76	532.0
77	290.5
78	141.0
79	46.5
80	14.0
81	7.0
82	7.5
83	4.5
84	3.0
85	2.5
86	2.5
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0058317616293941995
2	0.0961357068603165
3	4.4180012343895454E-4
4	0.0
5	0.03958529106013032
6	0.005699221592362513
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	5.522501542986932E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	3.9762011109505905E-4
24-25	0.0
26-27	0.0
28-29	2.2090006171947722E-5
30-31	1.8776505246155566E-4
32-33	0.0018776505246155565
34-35	0.0013806253857467329
36-37	6.516551820724579E-4
38-39	6.627001851584318E-4
40-41	0.001678840469068027
42-43	6.406101789864841E-4
44-45	4.638901296109022E-4
46-47	2.540350709773988E-4
48-49	6.737451882444056E-4
50-51	6.516551820724579E-4
52-53	0.0011707703271132293
54-55	0.0016346604567241316
56-57	0.0010603202962534907
58-59	0.003114690870244629
60-61	0.0019549655462173738
62-63	0.001203905336371151
64-65	0.004892936367086421
66-67	0.004826666348570578
68-69	0.0040535161325524075
70-71	0.0018113805060997132
72-73	0.005224286459665637
74-75	0.004086651141810329
76-77	0.00383261607083293
78-79	6.627001851584318E-4
80-81	5.522501542986932E-5
82-83	1.435850401176602E-4
84-85	8.836002468779089E-5
86-87	0.0
88-89	1.7672004937558178E-4
90-91	0.0
92-93	0.0
94-95	3.3135009257921585E-5
96-97	2.540350709773988E-4
98-99	5.412051512127192E-4
100	8.173302283620658E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4526934.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.87457855851994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.77511801390918	32.80506290580634
2	16.818627600791434	16.77643927044328
3	6.981843868354439	10.446495614866928
4	3.5903677685153546	7.16272317299188
5	2.050723382578177	5.113948222309453
6	1.288553799129165	3.8559646608928113
7	0.8548784118801568	2.9845690357909755
8	0.5802584931590493	2.315211824104753
9	0.43779950779823534	1.9651559350108008
>10	1.5808300039719188	12.123192220691577
>50	0.027112957638331343	0.8932938668271662
>100	0.011468702410778383	1.0591837047353418
>500	0.0013699109228092604	0.47079068970023225
>1k	8.058299545880173E-4	0.6614942770816212
>5k	8.058299545880174E-5	0.28567357199685056
>10k+	1.6116599091760348E-4	1.080801026750043
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13852	0.30599076549381987	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13040	0.2880536804821983	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7108	0.15701576387020444	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.2090006171947726E-5	0.0
9	0.0	0.0	0.0	2.2090006171947726E-5	0.0
10-11	0.0	0.0	0.0	2.2090006171947726E-5	1.1045003085973863E-5
12-13	0.0	0.0	0.0	3.313500925792159E-5	2.2090006171947726E-5
14-15	0.0	0.0	0.0	4.418001234389545E-5	2.2090006171947726E-5
16-17	0.0	0.0	0.0	5.522501542986931E-5	4.418001234389545E-5
18-19	0.0	0.0	0.0	8.83600246877909E-5	4.418001234389545E-5
20-21	0.0	0.0	0.0	1.4358504011766023E-4	4.418001234389545E-5
22-23	0.0	0.0	0.0	3.4239509566518975E-4	5.522501542986931E-5
24-25	0.0	0.0	0.0	8.06285225276092E-4	6.627001851584317E-5
26-27	0.0	0.0	0.0	0.0014468954042625758	6.627001851584317E-5
28-29	0.0	0.0	0.0	0.004329641209701754	6.627001851584317E-5
30-31	0.0	0.0	0.0	0.013695803826607589	7.731502160181704E-5
32-33	0.0	0.0	0.0	0.03584103501398518	1.1045003085973862E-4
34-35	0.0	0.0	0.0	0.06974919448792494	1.3254003703168634E-4
36-37	0.0	0.0	0.0	0.12137353891176678	1.3254003703168634E-4
38-39	0.0	0.0	0.0	0.1912994534490673	1.3254003703168634E-4
40-41	0.0	0.0	0.0	0.26450573390290205	1.3254003703168634E-4
42-43	0.0	0.0	0.0	0.3371708092055241	1.3254003703168634E-4
44-45	0.0	0.0	0.0	0.42847984971727004	1.3254003703168634E-4
46-47	0.0	0.0	0.0	0.5354948846172708	1.3254003703168634E-4
48-49	0.0	0.0	0.0	0.6496229015046386	1.5463004320363406E-4
50-51	0.0	0.0	0.0	0.7605257774909022	1.5463004320363406E-4
52-53	2.2090006171947726E-5	0.0	0.0	0.8698492180358715	1.8776505246155566E-4
54-55	2.2090006171947726E-5	0.0	0.0	0.9904385617285342	2.4299006789142496E-4
56-57	2.2090006171947726E-5	0.0	0.0	1.1319140062567734	2.4299006789142496E-4
58-59	2.2090006171947726E-5	0.0	0.0	1.286212699367828	2.4299006789142496E-4
60-61	2.2090006171947726E-5	0.0	0.0	1.4387000119727833	2.4299006789142496E-4
62-63	2.2090006171947726E-5	0.0	0.0	1.5894090790808968	2.4299006789142496E-4
64-65	2.2090006171947726E-5	0.0	0.0	1.7483356284849747	2.4299006789142496E-4
66-67	2.2090006171947726E-5	0.0	0.0	1.9281151437153712	2.540350709773988E-4
68-69	2.2090006171947726E-5	0.0	0.0	2.1248818736919954	2.650800740633727E-4
70-71	2.2090006171947726E-5	0.0	0.0	2.327579770325788	2.650800740633727E-4
72-73	2.2090006171947726E-5	0.0	0.0	2.531967552431734	3.7553010492311133E-4
74-75	2.2090006171947726E-5	0.0	0.0	2.7393264403678073	3.7553010492311133E-4
76-77	2.2090006171947726E-5	0.0	0.0	2.9641143431735477	3.9762011109505905E-4
78-79	2.2090006171947726E-5	0.0	0.0	3.211091657179009	4.1971011726700677E-4
80-81	2.2090006171947726E-5	0.0	0.0	3.4729576353443634	4.307551203529806E-4
82-83	2.2090006171947726E-5	0.0	0.0	3.7379824843923064	4.638901296109022E-4
84-85	2.2090006171947726E-5	0.0	0.0	4.0048739389617785	4.638901296109022E-4
86-87	2.2090006171947726E-5	0.0	0.0	4.288399168178728	4.638901296109022E-4
88	2.2090006171947726E-5	0.0	0.0	4.505234668762567	4.859801357828499E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8160	0.0	56.64326	1
GTATCAA	15315	0.0	42.811558	1
ATCAACG	18810	0.0	34.320793	3
CAACGCA	18840	0.0	34.21623	5
TATCAAC	19840	0.0	32.973972	2
AACGCAG	20395	0.0	32.169937	6
TCAACGC	20200	0.0	32.098778	4
ACGCAGA	21850	0.0	29.553202	7
CGCAGAG	22200	0.0	29.087276	8
GCAGAGT	24790	0.0	25.801853	9
GTGGTAT	2810	0.0	25.627842	1
GTACTAG	1980	0.0	24.24722	1
TACATGG	12640	0.0	24.053793	2
GTACATG	12930	0.0	23.989094	1
ACATGGG	12545	0.0	23.087963	3
AGAGTAC	22820	0.0	22.921804	10-11
GAGTACT	18210	0.0	22.724205	12-13
CAGAGTA	24040	0.0	22.384132	10-11
TGGTATC	3305	0.0	21.360819	2
TAGTACT	2760	0.0	21.124584	4
>>END_MODULE