FastQCFastQC Report
Mon 16 Jan 2017
ERR1146536_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146536_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3883691
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36350.043.3303261
GTATCAA58250.033.7389761
CAACGCA59500.030.8811325
ATCAACG62700.029.529913
AACGCAG68350.027.7760626
TATCAAC71500.027.0784992
TCAACGC69100.026.930884
ACGCAGA77100.023.7686487
GTACATG120700.023.7225631
TACATGG118700.023.318362
CGCAGAG79400.023.0807328
ACATGGG120350.022.6081943
CATGGGG90850.019.6558634
GTACTAG15450.019.4761491
GTATAAG19700.019.3317151
GCATCGT12800.018.7225788
GCAGAGT98700.018.2342179
TGATCGC8350.018.0081449
CGACGAG7350.017.26174424-25
CAGAGTA92250.017.14063810-11
Produced by FastQC (version 0.11.5)