##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146536_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3883691
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.472019272388046	33.0	31.0	34.0	28.0	34.0
2	31.54837807642266	33.0	31.0	34.0	28.0	34.0
3	31.741431540253846	33.0	31.0	34.0	30.0	34.0
4	35.25039942673091	37.0	35.0	37.0	33.0	37.0
5	35.23688316088999	37.0	35.0	37.0	32.0	37.0
6	35.32092228758673	37.0	35.0	37.0	32.0	37.0
7	35.19411791514825	37.0	35.0	37.0	32.0	37.0
8	35.15364893859991	37.0	35.0	37.0	32.0	37.0
9	36.79592866682751	39.0	37.0	39.0	33.0	39.0
10-11	36.80720685554026	39.0	37.0	39.0	33.0	39.0
12-13	36.783560149352766	39.0	37.0	39.0	33.0	39.0
14-15	38.19714300133558	40.0	38.0	41.0	33.0	41.0
16-17	38.19504486839968	40.0	38.0	41.0	33.0	41.0
18-19	38.210640728111485	40.0	38.5	41.0	33.0	41.0
20-21	38.30969469507229	40.0	39.0	41.0	33.0	41.0
22-23	38.314609478457484	40.0	39.0	41.0	33.0	41.0
24-25	38.36178187193575	40.5	38.5	41.0	33.0	41.0
26-27	38.391948921785996	40.0	38.5	41.0	33.0	41.0
28-29	38.35673705760834	40.0	38.0	41.0	33.0	41.0
30-31	38.34354458168788	40.0	38.0	41.0	33.0	41.0
32-33	38.36481854503873	40.0	38.0	41.0	33.0	41.0
34-35	38.31233200581612	40.0	38.0	41.0	33.0	41.0
36-37	38.22458982962341	40.0	38.0	41.0	33.0	41.0
38-39	38.190504728620276	40.0	38.0	41.0	33.0	41.0
40-41	38.13175430280113	40.0	38.0	41.0	33.0	41.0
42-43	38.01453668687854	40.0	38.0	41.0	32.5	41.0
44-45	37.87941870246629	40.0	38.0	41.0	32.0	41.0
46-47	37.78132297342914	40.0	37.5	41.0	32.0	41.0
48-49	37.67113951650633	40.0	37.0	41.0	32.0	41.0
50-51	37.551867154209745	40.0	37.0	41.0	31.0	41.0
52-53	37.4424923352553	40.0	37.0	41.0	31.0	41.0
54-55	37.2762377336405	40.0	36.0	41.0	31.0	41.0
56-57	37.15553309982694	39.5	36.0	41.0	31.0	41.0
58-59	36.94487962610826	39.0	35.0	41.0	31.0	41.0
60-61	36.7673360213261	39.0	35.0	41.0	31.0	41.0
62-63	36.535656029277305	39.0	35.0	41.0	30.5	41.0
64-65	36.28492560298953	38.0	35.0	40.0	30.0	41.0
66-67	36.018795522094834	37.0	35.0	40.0	30.0	41.0
68-69	35.717129143384476	37.0	35.0	40.0	30.0	41.0
70-71	35.43492067211321	36.5	35.0	39.0	30.0	41.0
72-73	35.10762197610469	36.0	34.0	39.0	29.0	41.0
74-75	34.7774111019646	35.5	34.0	38.5	29.0	40.0
76-77	34.458477000358684	35.0	34.0	37.0	29.0	39.5
78-79	34.174600399465355	35.0	34.0	37.0	29.0	39.0
80-81	33.89117723320419	35.0	34.0	36.5	29.0	39.0
82-83	33.64094993654233	35.0	34.0	36.0	28.5	37.5
84-85	33.4218373706868	35.0	34.0	36.0	28.0	37.0
86-87	33.23019570815495	35.0	34.0	35.0	28.0	37.0
88-89	33.08189091253655	35.0	34.0	35.0	28.0	36.0
90-91	32.92912489690863	35.0	33.5	35.0	27.5	36.0
92-93	32.84557036077278	35.0	33.5	35.0	27.0	36.0
94-95	32.75245700546208	35.0	33.0	35.0	27.0	36.0
96-97	32.68264043148644	35.0	33.0	35.0	27.0	35.5
98-99	32.615784443201065	35.0	33.0	35.0	27.0	35.0
100	31.724913490800375	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22167169362625216
1101	2	0.23894546306011932
1101	3	0.3287771858638173
1101	4	0.28739628156476726
1101	5	0.31074876366629667
1101	6	0.26820014156702143
1101	7	0.2533500344886974
1101	8	0.3409423908268536
1101	9	0.4521272466900612
1101	10-11	0.4683100313497661
1101	12-13	0.41311662460848453
1101	14-15	0.5086785022794942
1101	16-17	0.49592901843233506
1101	18-19	0.5100603447868295
1101	20-21	0.5199532385954058
1101	22-23	0.46974280998966833
1101	24-25	0.4659598176746442
1101	26-27	0.4628654180955536
1101	28-29	0.4692957710878076
1101	30-31	0.45835232838421547
1101	32-33	0.5017832388332337
1101	34-35	0.4701892305048432
1101	36-37	0.491947162187067
1101	38-39	0.5219471900714154
1101	40-41	0.5336598472679057
1101	42-43	0.4594713390988687
1101	44-45	0.4063495644598589
1101	46-47	0.3933723531503901
1101	48-49	0.3642794257726507
1101	50-51	0.3742676249433785
1101	52-53	0.33465626272174376
1101	54-55	0.43520722557556724
1101	56-57	0.38896760937453934
1101	58-59	0.4076516360252569
1101	60-61	0.4021114088284037
1101	62-63	0.341949795173079
1101	64-65	0.36251159215853335
1101	66-67	0.38998203546203314
1101	68-69	0.3595281815435811
1101	70-71	0.3193696421232062
1101	72-73	0.2629202633597032
1101	74-75	0.3619114737381892
1101	76-77	0.3341803089087634
1101	78-79	0.25943438793826346
1101	80-81	0.26525573550844683
1101	82-83	0.21903864580283283
1101	84-85	0.2790504932885369
1101	86-87	0.27209809145998776
1101	88-89	0.25093446358250304
1101	90-91	0.2699416046731571
1101	92-93	0.2623490387415828
1101	94-95	0.27742414895082135
1101	96-97	0.2863190770636095
1101	98-99	0.19955860229136846
1101	100	0.1567405296775597
1102	1	0.25111846540792726
1102	2	0.22724967718980693
1102	3	0.2941475073662083
1102	4	0.22005102200252225
1102	5	0.2401356110955959
1102	6	0.28562447561871807
1102	7	0.22711755365715902
1102	8	0.3213588559131324
1102	9	0.39653366574270166
1102	10-11	0.3893933701807484
1102	12-13	0.41674690418215476
1102	14-15	0.48423000790175763
1102	16-17	0.45420966598184975
1102	18-19	0.4520734548423917
1102	20-21	0.41663802000896766
1102	22-23	0.3951974653971604
1102	24-25	0.41635157083761953
1102	26-27	0.35159808218199373
1102	28-29	0.4572052999761098
1102	30-31	0.44608432908791684
1102	32-33	0.41906449662156575
1102	34-35	0.401399464191492
1102	36-37	0.45556350522557665
1102	38-39	0.4210291746486874
1102	40-41	0.40591033655849174
1102	42-43	0.36405394857533935
1102	44-45	0.3223638436816856
1102	46-47	0.3522595744196906
1102	48-49	0.3255530839802745
1102	50-51	0.4198671708377333
1102	52-53	0.36993122753680296
1102	54-55	0.37377376909493876
1102	56-57	0.3654060687139449
1102	58-59	0.3473994864452763
1102	60-61	0.37808279724082894
1102	62-63	0.3159981524761548
1102	64-65	0.3164692566114766
1102	66-67	0.3165490481139486
1102	68-69	0.3137506493273676
1102	70-71	0.2295094564493354
1102	72-73	0.23114340552153578
1102	74-75	0.2639759318167876
1102	76-77	0.2163274790865941
1102	78-79	0.20837705297762454
1102	80-81	0.18041581782654958
1102	82-83	0.22363857286738664
1102	84-85	0.2093506373268852
1102	86-87	0.1797312078195219
1102	88-89	0.1725581753005514
1102	90-91	0.22162507581418112
1102	92-93	0.19172947224937786
1102	94-95	0.15747763189344965
1102	96-97	0.1275653569891304
1102	98-99	0.12435605981287523
1102	100	0.07135061822632593
1103	1	0.167395160950786
1103	2	0.19921135299539472
1103	3	0.2624624384068248
1103	4	0.2007280489812402
1103	5	0.2743980871169569
1103	6	0.21846911754897036
1103	7	0.22316229387779174
1103	8	0.22923459823849868
1103	9	0.3816350039308247
1103	10-11	0.393426296097779
1103	12-13	0.40990328995648184
1103	14-15	0.45885247014798125
1103	16-17	0.4331189256860952
1103	18-19	0.38777975171642254
1103	20-21	0.42578995330183744
1103	22-23	0.40562469590301475
1103	24-25	0.38548468324388807
1103	26-27	0.38957490022578867
1103	28-29	0.4208820975201135
1103	30-31	0.33309248691727333
1103	32-33	0.39557029199748683
1103	34-35	0.3831893852650481
1103	36-37	0.39310844750261253
1103	38-39	0.3093035790121945
1103	40-41	0.31082695944249394
1103	42-43	0.34439287613325575
1103	44-45	0.29251702415810854
1103	46-47	0.28750824634570193
1103	48-49	0.21613705187932908
1103	50-51	0.16641648640277396
1103	52-53	0.29876523084663376
1103	54-55	0.21543972302065129
1103	56-57	0.20413756629808688
1103	58-59	0.2841150471416185
1103	60-61	0.24441207722765768
1103	62-63	0.26685194826296765
1103	64-65	0.22475635228936852
1103	66-67	0.19189240824182008
1103	68-69	0.17820496806240982
1103	70-71	0.1481445891933788
1103	72-73	0.20471474455588634
1103	74-75	0.16453120227596685
1103	76-77	0.1567940370546097
1103	78-79	0.15843257889221007
1103	80-81	0.17113445982650433
1103	82-83	0.18108765487965428
1103	84-85	0.20081217035802013
1103	86-87	0.2022854428810419
1103	88-89	0.1184203520159599
1103	90-91	0.22287401292508235
1103	92-93	0.17732147036321066
1103	94-95	0.12256899397083743
1103	96-97	0.17328231556282248
1103	98-99	0.06663784455243871
1103	100	0.1326060650379759
1104	1	0.13580486155323968
1104	2	0.14115786080173365
1104	3	0.15527762003349466
1104	4	0.13361869772782597
1104	5	0.15358428278909741
1104	6	0.17290615859631941
1104	7	0.07689049048990881
1104	8	0.17307494617030272
1104	9	0.2205993429386197
1104	10-11	0.2674393739254626
1104	12-13	0.20902259786925015
1104	14-15	0.3463433153756057
1104	16-17	0.279511801583034
1104	18-19	0.2566363148132993
1104	20-21	0.29717415054509644
1104	22-23	0.30583254952301786
1104	24-25	0.3115146649945686
1104	26-27	0.28874305167744296
1104	28-29	0.29643400183756086
1104	30-31	0.2857468093317266
1104	32-33	0.23518871772063932
1104	34-35	0.25459333261733974
1104	36-37	0.2691394004811869
1104	38-39	0.2763685359271193
1104	40-41	0.2955066262386197
1104	42-43	0.25747383996895934
1104	44-45	0.20356551729533123
1104	46-47	0.13929355311101688
1104	48-49	0.08352874287822942
1104	50-51	0.15392964274257537
1104	52-53	0.1666423187559971
1104	54-55	0.1889884128450774
1104	56-57	0.19656573260138543
1104	58-59	0.19183684059476747
1104	60-61	0.12938424096821421
1104	62-63	0.16570876420465197
1104	64-65	0.16905702968556113
1104	66-67	0.10427707221703741
1104	68-69	0.15038308713364756
1104	70-71	0.18151961478984902
1104	72-73	0.12516532050487683
1104	74-75	0.1260952654984493
1104	76-77	0.09532068326146259
1104	78-79	0.17255238761131153
1104	80-81	0.1488331684406603
1104	82-83	0.17470549395945767
1104	84-85	0.12612724156855393
1104	86-87	0.11245174639528699
1104	88-89	0.1222383085329497
1104	90-91	0.11479240938117385
1104	92-93	0.16755580106620016
1104	94-95	0.1211536599210774
1104	96-97	0.07430057475565377
1104	98-99	0.10723728640567742
1104	100	0.022805225354492364
1105	1	0.10955606384552752
1105	2	0.18022181016193883
1105	3	0.1728660860847917
1105	4	0.11818202013352419
1105	5	0.1333114442714347
1105	6	0.1323071023013398
1105	7	0.08422480076441019
1105	8	0.14995437450865268
1105	9	0.23697277618181545
1105	10-11	0.22473798052535443
1105	12-13	0.19806308049833632
1105	14-15	0.25757426124600613
1105	16-17	0.19834551511770115
1105	18-19	0.1966320344009702
1105	20-21	0.21897595234688794
1105	22-23	0.26884233404202007
1105	24-25	0.25709476158081657
1105	26-27	0.30040727599119066
1105	28-29	0.2689153316861379
1105	30-31	0.32593816894175376
1105	32-33	0.31555119310826996
1105	34-35	0.3278471006391257
1105	36-37	0.30356272822480435
1105	38-39	0.2875508361703538
1105	40-41	0.3091031797513324
1105	42-43	0.2953619348833314
1105	44-45	0.2548341811520345
1105	46-47	0.18522026238037625
1105	48-49	0.1816493900765721
1105	50-51	0.17089785186841056
1105	52-53	0.17854554115111654
1105	54-55	0.21773017305133635
1105	56-57	0.16549552880339746
1105	58-59	0.19783702188281183
1105	60-61	0.159510588668617
1105	62-63	0.2274610139390134
1105	64-65	0.12523235537458532
1105	66-67	0.13407136063626268
1105	68-69	0.10325735883407816
1105	70-71	0.1229693149091986
1105	72-73	0.14466405904218504
1105	74-75	0.15260053544139396
1105	76-77	0.1420021047612181
1105	78-79	0.11236369696426607
1105	80-81	0.07495476236549337
1105	82-83	0.08596464019345262
1105	84-85	0.14258663556288553
1105	86-87	0.15589334898167095
1105	88-89	0.09728496502734174
1105	90-91	0.19514919433829903
1105	92-93	0.17958663010653453
1105	94-95	0.20556253497835542
1105	96-97	0.13763728206105696
1105	98-99	0.1314597369165611
1105	100	0.1287545956303049
1106	1	0.1193609240562914
1106	2	0.07753981345780048
1106	3	0.11246203416782308
1106	4	0.118064242893972
1106	5	0.10008439539862479
1106	6	0.09479296190897202
1106	7	0.004553627218697898
1106	8	0.0833863937709296
1106	9	0.039217857319883365
1106	10-11	0.11378659688025294
1106	12-13	0.1552790260168635
1106	14-15	0.12993987409132757
1106	16-17	0.08835990483688505
1106	18-19	0.04230144128809599
1106	20-21	0.03518153061889251
1106	22-23	0.06689492537874742
1106	24-25	0.06410132006338642
1106	26-27	0.045758114441653674
1106	28-29	0.06601786912077756
1106	30-31	0.08519921174522693
1106	32-33	0.06870862043543724
1106	34-35	0.05825746883547822
1106	36-37	0.08967237503555481
1106	38-39	0.061296912241147083
1106	40-41	0.10470139789567412
1106	42-43	0.002407958239317054
1106	44-45	-0.03374494093262825
1106	46-47	-0.01940980785854407
1106	48-49	0.03879177231121389
1106	50-51	0.039717799913454144
1106	52-53	-0.06105890778912482
1106	54-55	-0.05123756643300226
1106	56-57	-0.07004009643701892
1106	58-59	-0.02345705553672417
1106	60-61	-0.05063905491275733
1106	62-63	-0.019888285808804085
1106	64-65	-0.11153703394093384
1106	66-67	-0.06567722294995804
1106	68-69	-0.055506170113957864
1106	70-71	-0.041003292384445444
1106	72-73	-0.022284798091178004
1106	74-75	-0.001953454500046803
1106	76-77	-0.10239920218621279
1106	78-79	-0.11042130733365241
1106	80-81	-0.06713255426056719
1106	82-83	-0.05681124586656239
1106	84-85	-0.027106478742780382
1106	86-87	-0.0420202367178959
1106	88-89	-0.07025871465655342
1106	90-91	-0.08255296221994257
1106	92-93	-0.03090746440970804
1106	94-95	-0.08771746616142195
1106	96-97	-0.05099066058902224
1106	98-99	-0.019991392652649154
1106	100	-0.12345662455139461
1107	1	0.05668017368456901
1107	2	0.0661872604712812
1107	3	0.1401589619749508
1107	4	0.06890151791183996
1107	5	0.015842558748246915
1107	6	0.07988627214295718
1107	7	0.07104499373844675
1107	8	0.13054979066967576
1107	9	0.10117932174582478
1107	10-11	0.2156559085473546
1107	12-13	0.18296855332236106
1107	14-15	0.24085033578905524
1107	16-17	0.16892337108016875
1107	18-19	0.14335056636836896
1107	20-21	0.12450330705976853
1107	22-23	0.16410785365143
1107	24-25	0.13095232955602398
1107	26-27	0.13698018374056176
1107	28-29	0.12768401683491248
1107	30-31	0.17293515548625038
1107	32-33	0.12885382745162133
1107	34-35	0.11885684341358171
1107	36-37	0.1460908637393672
1107	38-39	0.1711377262278262
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2216	50-51	-0.46851096855721863
2216	52-53	-0.43730110538695754
2216	54-55	-0.39090355015125056
2216	56-57	-0.3736284867522883
2216	58-59	-0.392559442911697
2216	60-61	-0.4056705441996016
2216	62-63	-0.41761419956081625
2216	64-65	-0.3449119402225378
2216	66-67	-0.36717529274162786
2216	68-69	-0.330354183836576
2216	70-71	-0.4538347586871794
2216	72-73	-0.3696169301899985
2216	74-75	-0.35839294719525583
2216	76-77	-0.32092220783771097
2216	78-79	-0.3224960868976865
2216	80-81	-0.30181650456303544
2216	82-83	-0.2601171492966827
2216	84-85	-0.26484953433803327
2216	86-87	-0.24551938967890408
2216	88-89	-0.2546530955929285
2216	90-91	-0.23606035306159612
2216	92-93	-0.3051024271720024
2216	94-95	-0.3064129003267979
2216	96-97	-0.2914339448057248
2216	98-99	-0.2958555148862203
2216	100	-0.25575870291770286
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	103.0
1	50.0
2	40.0
3	40.0
4	36.0
5	53.0
6	120.0
7	499.0
8	1526.0
9	2131.0
10	2521.0
11	3736.0
12	5307.0
13	6826.0
14	7578.0
15	8433.0
16	9187.0
17	10015.0
18	11139.0
19	12603.0
20	13984.0
21	15591.0
22	17386.0
23	20172.0
24	24192.0
25	30370.0
26	34440.0
27	32670.0
28	35132.0
29	44650.0
30	50818.0
31	55543.0
32	70110.0
33	94208.0
34	138117.0
35	213370.0
36	401742.0
37	910797.0
38	1209609.0
39	387601.0
40	1246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64801701450123	19.89068363307522	13.35674416028258	24.10455519214097
2	14.983655813632499	20.873562898722696	40.21465553603626	23.92812575160854
3	17.831189903954485	26.105185252711443	30.574107393704725	25.489517449629346
4	12.164204490294855	17.74814187636303	39.854682281008195	30.232971352333927
5	13.301768650584062	38.40272164270946	35.41153618251549	12.883973524190992
6	29.89985432279154	38.57420202650207	18.693318960466808	12.832624690239575
7	26.588369373159875	32.75735186197044	23.244722515909313	17.409556248960364
8	25.340459899930394	35.66399047044455	20.993784725467794	18.00176490415726
9	25.99657828964953	15.837204044457515	20.80074408294918	37.36547358294378
10-11	24.133679125653398	26.52802013468184	29.363245064277276	19.97505567538748
12-13	24.888543722520573	24.364026173990887	28.589122598622087	22.158307504866457
14-15	22.459125540274975	25.721601612639276	25.92291113526854	25.89636171181721
16-17	22.389158924086384	28.440480962078663	26.633840984626257	22.5365191292087
18-19	22.291122202746198	28.281609421829852	27.722564232901675	21.704704142522274
20-21	23.315509811520442	27.4457472682116	27.17109422175414	22.06764869851382
22-23	23.2991425481512	27.36789319188382	26.79967040889896	22.533293851066023
24-25	22.946582597678187	27.530435053539197	27.0998117318494	22.423170616933216
26-27	22.80871658232517	27.58487633955557	26.858151147767455	22.748255930351803
28-29	22.635188505759853	27.816848593482824	27.004547859256483	22.543415041500843
30-31	23.029720827600556	27.4593259310017	26.926683028739724	22.584270212658023
32-33	22.942568546284434	27.68336412739083	26.647937012740368	22.726130313584363
34-35	23.11389634002431	27.760490628565382	26.84089040056128	22.28472263084903
36-37	23.12196017654218	27.444838294139796	26.873043735343305	22.560157793974717
38-39	23.002941265091657	27.54544590644969	26.904167191386712	22.54744563707194
40-41	22.98056523725471	27.28281074477175	27.0885687046393	22.648055313334236
42-43	22.92568853118712	27.41389134808853	26.90189134808853	22.758528772635813
44-45	22.824406827330595	27.619715300813784	26.973225439933636	22.582652431921986
46-47	22.921335754916797	27.43572675256051	26.79607534519219	22.84686214733051
48-49	23.059902056120887	27.317719028277825	26.903349993863536	22.71902892173775
50-51	22.963855288248613	27.449841796135754	26.71018770039489	22.876115215220747
52-53	22.820917094187813	27.527184729958208	26.794329973047397	22.857568202806576
54-55	22.824857723841617	27.431889100517438	26.84085174186148	22.902401433779463
56-57	22.954039476866733	27.389900064988538	26.77236727514107	22.883693183003658
58-59	22.844920403182016	27.350710246152037	27.023328343184843	22.7810410074811
60-61	22.88580223063648	27.491023362439794	26.902763825567316	22.72041058135641
62-63	22.93745696200396	27.387110861499142	26.710685889306824	22.964746287190078
64-65	22.60490079450171	27.43714181838132	26.867914847385123	23.090042539731847
66-67	22.894007215306793	27.381795645985296	26.73466670032367	22.989530438384236
68-69	22.796994549629996	27.350427790743602	26.85770630595149	22.994871353674906
70-71	22.80844759035392	27.19598930457178	26.807062300315582	23.18850080475872
72-73	22.947160437097356	27.28570547301536	26.887354923243695	22.879779166643594
74-75	22.80628501513298	27.535243737523345	26.832970571189385	22.82550067615429
76-77	22.857081773681212	27.350013239656324	26.72838046547953	23.064524521182936
78-79	22.90281426103818	27.40880530036592	26.67346762149634	23.014912817099564
80-81	22.780539008919085	27.550761503042793	26.7704414463728	22.898258041665326
82-83	23.027181993981593	27.220116130885184	27.07656563223846	22.67613624289476
84-85	23.18628735686215	27.377030073517624	26.63714027589875	22.79954229372148
86-87	22.745153188989857	27.505930172643716	26.8891072203804	22.859809417986025
88-89	23.10437225752754	27.266716710973775	26.656333277056028	22.972577754442653
90-91	23.02432759822824	27.307884196191957	26.752270401546646	22.91551780403316
92-93	23.20423830783986	27.232212252297128	26.800043838879496	22.763505600983517
94-95	22.795457545696614	27.224189502899403	26.966929722037086	23.013423229366897
96-97	22.94111927530623	27.23803869575212	26.84456470015525	22.976277328786395
98-99	22.864507661055505	27.35851535031406	26.759335788586856	23.017641200043577
100	23.11894257243157	27.081900670421856	26.593958219042246	23.205198538104327
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	976.0
1	743.0
2	582.0
3	646.5
4	589.5
5	516.5
6	482.5
7	509.0
8	517.5
9	489.0
10	464.0
11	457.5
12	474.5
13	493.0
14	509.5
15	585.5
16	692.5
17	814.0
18	1100.5
19	1513.5
20	2133.5
21	3311.0
22	5029.5
23	7133.0
24	9801.5
25	13287.0
26	18153.5
27	23469.0
28	28656.0
29	35990.5
30	43497.0
31	50697.0
32	60421.0
33	69520.0
34	77201.5
35	85214.5
36	95213.5
37	106322.5
38	111514.0
39	113565.5
40	115884.5
41	117928.5
42	121556.5
43	128035.0
44	135007.5
45	145243.0
46	155232.5
47	158945.0
48	160697.5
49	157520.5
50	152118.0
51	150784.0
52	147805.5
53	142384.5
54	136724.5
55	126455.0
56	115317.0
57	103828.5
58	91745.5
59	80707.0
60	65646.5
61	50727.0
62	40349.5
63	31467.0
64	23094.5
65	15887.0
66	11198.0
67	8082.5
68	5636.0
69	3984.5
70	2958.5
71	2142.0
72	1627.5
73	1244.5
74	838.5
75	571.0
76	421.0
77	308.5
78	229.0
79	138.5
80	93.5
81	67.5
82	46.0
83	40.5
84	32.0
85	23.5
86	22.5
87	18.0
88	10.5
89	6.5
90	6.5
91	6.5
92	5.0
93	5.0
94	5.5
95	5.0
96	4.0
97	2.5
98	3.5
99	5.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0591190184801005
2	0.009089291604301166
3	0.011020444211447307
4	0.008703061082871938
5	0.01153541824001961
6	0.01153541824001961
7	0.0030125980671479786
8	0.004995248077151349
9	0.0065144214614396454
10-11	0.0062440600964391866
12-13	0.005600342560723806
14-15	0.009462647775016087
16-17	0.008999171149301013
18-19	0.007312631205726717
20-21	0.006334180551439339
22-23	0.002343131830003983
24-25	0.0034632003421487447
26-27	0.004467399697864738
28-29	0.005548845157866576
30-31	0.008239584457156864
32-33	0.009424024722873164
34-35	0.011007569860732999
36-37	0.016813902032885727
38-39	0.017200132554314955
40-41	0.016710907227171265
42-43	0.022620234205038454
44-45	0.02655978552361658
46-47	0.028516686832191333
48-49	0.029907116709336556
50-51	0.03091131606505255
52-53	0.029211901770763948
54-55	0.03513410309934544
56-57	0.03779909369720712
58-59	0.035404464464345906
60-61	0.03581644368720375
62-63	0.031426290093624853
64-65	0.034001160236486375
66-67	0.03576494628434651
68-69	0.038043706360778966
70-71	0.037889214152207265
72-73	0.033640678416485756
74-75	0.03710387875863451
76-77	0.03643441252149051
78-79	0.03586794109006097
80-81	0.04032246643721141
82-83	0.03691076349791989
84-85	0.03317720179077069
86-87	0.031297546586481775
88-89	0.0291346556664781
90-91	0.031619405354339464
92-93	0.03328019659648515
94-95	0.036782019990776814
96-97	0.0315035361979107
98-99	0.02914753001719241
100	0.02731937221576073
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3883691.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.89404809855931
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.0354901857354	39.145670570476724
2	15.07902210007172	16.85655173081295
3	6.324913981343122	10.605751388773282
4	3.1033755578145086	6.9384089078550995
5	1.7318966991916362	4.840135870317671
6	1.042365048516594	3.495720129482617
7	0.6599196065185976	2.5819904759552976
8	0.4701051746845949	2.10208649961619
9	0.31650853411427465	1.5921848906448992
>10	1.2055114949902055	10.332035956983058
>50	0.023420189005071496	0.8623561527982765
>100	0.007471428014279145	0.6471074262838818
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.57487014286152E-5
2	0.0	0.0	0.0	2.57487014286152E-5	2.57487014286152E-5
3	0.0	0.0	0.0	5.14974028572304E-5	2.57487014286152E-5
4	0.0	0.0	0.0	5.14974028572304E-5	2.57487014286152E-5
5	0.0	0.0	0.0	5.14974028572304E-5	2.57487014286152E-5
6	0.0	0.0	0.0	5.14974028572304E-5	2.57487014286152E-5
7	0.0	0.0	0.0	7.724610428584561E-5	2.57487014286152E-5
8	0.0	0.0	0.0	1.5449220857169122E-4	2.57487014286152E-5
9	0.0	0.0	0.0	1.5449220857169122E-4	2.57487014286152E-5
10-11	1.28743507143076E-5	0.0	0.0	1.5449220857169122E-4	2.57487014286152E-5
12-13	2.57487014286152E-5	0.0	0.0	2.059896114289216E-4	2.9611006642907484E-4
14-15	2.57487014286152E-5	0.0	0.0	2.059896114289216E-4	3.4760746928630523E-4
16-17	2.57487014286152E-5	0.0	0.0	2.57487014286152E-4	4.248535735721508E-4
18-19	2.57487014286152E-5	0.0	0.0	3.2185876785769004E-4	4.634766257150736E-4
20-21	2.57487014286152E-5	0.0	0.0	3.3473311857199763E-4	4.763509764293812E-4
22-23	2.57487014286152E-5	0.0	0.0	4.50602275000766E-4	5.14974028572304E-4
24-25	2.57487014286152E-5	0.0	0.0	7.595866921441484E-4	5.14974028572304E-4
26-27	2.57487014286152E-5	0.0	0.0	0.0011715659150019916	5.407227300009192E-4
28-29	2.57487014286152E-5	0.0	0.0	0.0032185876785769	5.407227300009192E-4
30-31	2.57487014286152E-5	0.0	0.0	0.009617139983587777	5.407227300009192E-4
32-33	2.57487014286152E-5	0.0	0.0	0.022620234205038454	5.407227300009192E-4
34-35	2.57487014286152E-5	0.0	0.0	0.042614100864358156	5.922201328581497E-4
36-37	2.57487014286152E-5	0.0	0.0	0.0751990825222707	5.922201328581497E-4
38-39	2.57487014286152E-5	0.0	0.0	0.11916499021163116	5.922201328581497E-4
40-41	2.57487014286152E-5	0.0	0.0	0.16483031219528022	5.922201328581497E-4
42-43	2.57487014286152E-5	0.0	0.0	0.20980041924035667	5.922201328581497E-4
44-45	2.57487014286152E-5	0.0	0.0	0.2670655312175969	6.050944835724573E-4
46-47	2.57487014286152E-5	0.0	0.0	0.3360849253969999	6.694662371439953E-4
48-49	2.57487014286152E-5	0.0	0.0	0.40829715855355125	7.467123414298409E-4
50-51	2.57487014286152E-5	0.0	0.0	0.48403696380582284	8.239584457156864E-4
52-53	2.57487014286152E-5	0.0	0.0	0.5569830349530898	8.497071471443016E-4
54-55	2.57487014286152E-5	0.0	0.0	0.6368297580832254	8.497071471443016E-4
56-57	2.57487014286152E-5	0.0	0.0	0.7298598163448122	8.625814978586092E-4
58-59	2.57487014286152E-5	0.0	0.0	0.8325842606942726	8.754558485729168E-4
60-61	2.57487014286152E-5	0.0	0.0	0.939248256362311	8.754558485729168E-4
62-63	2.57487014286152E-5	0.0	0.0	1.0419469520103428	9.527019528587624E-4
64-65	2.57487014286152E-5	0.0	0.0	1.1491902934605251	9.784506542873776E-4
66-67	2.57487014286152E-5	0.0	0.0	1.2689346294543	9.784506542873776E-4
68-69	2.57487014286152E-5	0.0	0.0	1.4013859496030965	0.0010041993557159928
70-71	2.57487014286152E-5	0.0	0.0	1.5416906236876209	0.001029948057144608
72-73	2.57487014286152E-5	0.0	0.0	1.6850465188914359	0.001132942862859069
74-75	2.57487014286152E-5	0.0	0.0	1.8264455127866763	0.0012230633178592222
76-77	2.57487014286152E-5	0.0	0.0	1.9805128677847954	0.0012359376685735298
78-79	2.57487014286152E-5	0.0	0.0	2.1486647624643673	0.0012874350714307601
80-81	2.57487014286152E-5	0.0	0.0	2.3310428146832485	0.0012874350714307601
82-83	2.57487014286152E-5	0.0	0.0	2.5175149104292798	0.0013131837728593753
84-85	2.57487014286152E-5	2.57487014286152E-5	0.0	2.6998929626481614	0.0013131837728593753
86-87	2.57487014286152E-5	2.57487014286152E-5	0.0	2.8910641964049146	0.0013518068250022981
88	2.57487014286152E-5	2.57487014286152E-5	0.0	3.0452731692608914	0.001390429877145221
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3635	0.0	43.330326	1
GTATCAA	5825	0.0	33.738976	1
CAACGCA	5950	0.0	30.881132	5
ATCAACG	6270	0.0	29.52991	3
AACGCAG	6835	0.0	27.776062	6
TATCAAC	7150	0.0	27.078499	2
TCAACGC	6910	0.0	26.93088	4
ACGCAGA	7710	0.0	23.768648	7
GTACATG	12070	0.0	23.722563	1
TACATGG	11870	0.0	23.31836	2
CGCAGAG	7940	0.0	23.080732	8
ACATGGG	12035	0.0	22.608194	3
CATGGGG	9085	0.0	19.655863	4
GTACTAG	1545	0.0	19.476149	1
GTATAAG	1970	0.0	19.331715	1
GCATCGT	1280	0.0	18.722578	8
GCAGAGT	9870	0.0	18.234217	9
TGATCGC	835	0.0	18.008144	9
CGACGAG	735	0.0	17.261744	24-25
CAGAGTA	9225	0.0	17.140638	10-11
>>END_MODULE