FastQCFastQC Report
Mon 16 Jan 2017
ERR1146536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3883691
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126340.32530909384912443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111610.28738125664477426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63790.16425096641313638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66200.058.70671
GTATCAA124150.043.7345541
CAACGCA153900.034.889195
ATCAACG156000.034.3291133
TATCAAC160600.033.6008222
TCAACGC161800.033.1857074
AACGCAG166250.032.5676546
ACGCAGA183200.029.3473037
CGCAGAG187300.028.654718
GCAGAGT208650.025.5874869
AGAGTAC190350.022.60336910-11
GAGTACT143500.022.59773312-13
CAGAGTA201900.022.4741710-11
GTACATG116200.022.4320661
GTGGTAT23800.021.7460981
TACATGG115900.021.6765352
GTACTAG14700.021.1247831
ACATGGG118750.020.9452483
GTACTTT157550.020.34387414-15
AGTACTT154200.020.2829612-13
Produced by FastQC (version 0.11.5)