##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146536_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3883691
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54961864885749	34.0	31.0	34.0	31.0	34.0
2	32.61904332759738	34.0	31.0	34.0	31.0	34.0
3	32.714876904470515	34.0	31.0	34.0	31.0	34.0
4	36.199322242681	37.0	37.0	37.0	35.0	37.0
5	36.21422224373669	37.0	37.0	37.0	35.0	37.0
6	36.28434084998008	37.0	37.0	37.0	35.0	37.0
7	36.27442656998201	37.0	37.0	37.0	35.0	37.0
8	36.28434007751904	37.0	37.0	37.0	35.0	37.0
9	38.09871279666688	39.0	39.0	39.0	37.0	39.0
10-11	38.060469666613535	39.0	38.0	39.0	36.0	39.0
12-13	38.03861944732472	39.0	38.0	39.0	35.0	39.0
14-15	39.506918676073866	41.0	39.0	41.0	37.0	41.0
16-17	39.505330753656764	41.0	39.0	41.0	37.0	41.0
18-19	39.55255490202491	41.0	39.5	41.0	37.0	41.0
20-21	39.58529051358617	41.0	40.0	41.0	37.0	41.0
22-23	39.547248480890985	41.0	39.5	41.0	37.0	41.0
24-25	39.53714726532054	41.0	39.0	41.0	37.0	41.0
26-27	39.46797273521503	41.0	39.0	41.0	36.0	41.0
28-29	39.384961367935816	41.0	39.0	41.0	36.0	41.0
30-31	39.288191568278734	41.0	39.0	41.0	36.0	41.0
32-33	39.175779303760265	41.0	39.0	41.0	35.0	41.0
34-35	39.105985388641884	41.0	39.0	41.0	35.0	41.0
36-37	39.027279590472055	41.0	39.0	41.0	35.0	41.0
38-39	38.935756989935605	41.0	39.0	41.0	35.0	41.0
40-41	38.850452700794165	40.5	39.0	41.0	35.0	41.0
42-43	38.76097480463817	40.0	38.0	41.0	35.0	41.0
44-45	38.62155884698345	40.0	38.0	41.0	35.0	41.0
46-47	38.40301455496846	40.0	38.0	41.0	34.5	41.0
48-49	38.20481907546198	40.0	38.0	41.0	34.0	41.0
50-51	38.064589201355105	40.0	38.0	41.0	34.0	41.0
52-53	37.94346460622124	40.0	37.5	41.0	33.5	41.0
54-55	37.81386534098619	40.0	37.0	41.0	33.0	41.0
56-57	37.63084022390041	40.0	37.0	41.0	33.0	41.0
58-59	37.431780875461	40.0	36.0	41.0	33.0	41.0
60-61	37.25164733754565	39.0	36.0	41.0	33.0	41.0
62-63	36.99858253398635	39.0	35.0	41.0	33.0	41.0
64-65	36.70170979102096	39.0	35.0	41.0	32.0	41.0
66-67	36.43956123182818	37.5	35.0	40.5	32.0	41.0
68-69	36.120685708518	37.0	35.0	40.0	32.0	41.0
70-71	35.827221063673704	37.0	35.0	39.5	31.5	41.0
72-73	35.49837010719956	36.0	35.0	39.0	31.0	41.0
74-75	35.16408102498371	36.0	35.0	39.0	31.0	40.5
76-77	34.85390199683754	35.0	35.0	37.5	31.0	39.5
78-79	34.562745079358784	35.0	35.0	37.0	31.0	39.0
80-81	34.293196214631905	35.0	35.0	37.0	31.0	39.0
82-83	34.040679729669534	35.0	34.5	36.0	31.0	38.0
84-85	33.83182338656705	35.0	34.0	36.0	30.5	37.0
86-87	33.64011387620694	35.0	34.0	35.5	30.0	37.0
88-89	33.48093978125448	35.0	34.0	35.0	30.0	36.5
90-91	33.39310104743143	35.0	34.0	35.0	30.0	36.0
92-93	33.30674891488535	35.0	34.0	35.0	30.0	36.0
94-95	33.22798595459834	35.0	34.0	35.0	30.0	36.0
96-97	33.180326009458526	35.0	34.0	35.0	30.0	36.0
98-99	33.124739069096904	35.0	34.0	35.0	30.0	35.0
100	32.59103337520931	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.27026112283851944
1101	2	0.2642605395749271
1101	3	0.2574396054867094
1101	4	0.13974277115774214
1101	5	0.15656501841991144
1101	6	0.1453277098206769
1101	7	0.15984429036179648
1101	8	0.16515372610403034
1101	9	0.21435017627222663
1101	10-11	0.21110758327394308
1101	12-13	0.2071159664532516
1101	14-15	0.33000236177700515
1101	16-17	0.29092186521808827
1101	18-19	0.2915943268024179
1101	20-21	0.3076767767323716
1101	22-23	0.3113585215889998
1101	24-25	0.3251715557994572
1101	26-27	0.3297722871624913
1101	28-29	0.3539742101674648
1101	30-31	0.36372667273471393
1101	32-33	0.3496958314931362
1101	34-35	0.3239083831882752
1101	36-37	0.3886036145347802
1101	38-39	0.39071771258493015
1101	40-41	0.36420183466726996
1101	42-43	0.362193780871678
1101	44-45	0.4205875433585433
1101	46-47	0.40847324912942895
1101	48-49	0.4252864937672385
1101	50-51	0.3900324751126334
1101	52-53	0.39876321156472017
1101	54-55	0.3435305226389076
1101	56-57	0.35840566256370465
1101	58-59	0.3794189562546251
1101	60-61	0.38946155380292424
1101	62-63	0.38782268576992607
1101	64-65	0.4011590329038697
1101	66-67	0.3113782828366283
1101	68-69	0.3577765810801665
1101	70-71	0.26321706439598813
1101	72-73	0.25656705291234516
1101	74-75	0.27229270923692894
1101	76-77	0.25023096834231495
1101	78-79	0.22290119457414193
1101	80-81	0.23136758446946004
1101	82-83	0.2315220676069174
1101	84-85	0.20244195665704012
1101	86-87	0.189777533498507
1101	88-89	0.22027141944055728
1101	90-91	0.21359746914251332
1101	92-93	0.18150669103945916
1101	94-95	0.21175759348339085
1101	96-97	0.12117395392272101
1101	98-99	0.15236663373928394
1101	100	0.19476451630492875
1102	1	0.23610699080702346
1102	2	0.23753876815975872
1102	3	0.21977339329219348
1102	4	0.1447355509146746
1102	5	0.16428554736685896
1102	6	0.17003780056850815
1102	7	0.1662078167724914
1102	8	0.15835588557977331
1102	9	0.20074808130220845
1102	10-11	0.20981632343315937
1102	12-13	0.2202878705449649
1102	14-15	0.30629375905896694
1102	16-17	0.2864531944758326
1102	18-19	0.3080645900297512
1102	20-21	0.3099459305345462
1102	22-23	0.2584718445867651
1102	24-25	0.2849796329424379
1102	26-27	0.29356890720911366
1102	28-29	0.2979930451061108
1102	30-31	0.30478617010317066
1102	32-33	0.2843588165688615
1102	34-35	0.31686498109770156
1102	36-37	0.32970251170678466
1102	38-39	0.32685658681342744
1102	40-41	0.34385595936856816
1102	42-43	0.37711691460377494
1102	44-45	0.3893134911528833
1102	46-47	0.4206422907795968
1102	48-49	0.3865335800143228
1102	50-51	0.3260146664014201
1102	52-53	0.35826204606355105
1102	54-55	0.3659336632613872
1102	56-57	0.35396241843885434
1102	58-59	0.4072088746695499
1102	60-61	0.3571938019007348
1102	62-63	0.36134281082307496
1102	64-65	0.36218850395692215
1102	66-67	0.3026072451740873
1102	68-69	0.24624787786514446
1102	70-71	0.2842574757665801
1102	72-73	0.24074574110046143
1102	74-75	0.23913250597059488
1102	76-77	0.23609124930872838
1102	78-79	0.26666040313287454
1102	80-81	0.2858439977312486
1102	82-83	0.25213503692271644
1102	84-85	0.204406449187573
1102	86-87	0.1910096936339727
1102	88-89	0.20643223844335523
1102	90-91	0.16970242288897452
1102	92-93	0.17026720987072963
1102	94-95	0.19841342978545384
1102	96-97	0.15619625604408327
1102	98-99	0.16261585124085087
1102	100	0.16828968086782936
1103	1	0.2226263440998224
1103	2	0.24304293848629044
1103	3	0.2258144951399359
1103	4	0.15695068645537447
1103	5	0.1081438472239924
1103	6	0.1245294035749609
1103	7	0.12672400413783436
1103	8	0.1312971842345263
1103	9	0.20483265265526995
1103	10-11	0.1705271569599418
1103	12-13	0.22070544623918664
1103	14-15	0.2973573450555733
1103	16-17	0.297041074530803
1103	18-19	0.28619311555198834
1103	20-21	0.2547580588061962
1103	22-23	0.2831264028027647
1103	24-25	0.2847758229686761
1103	26-27	0.29825534870394677
1103	28-29	0.32805679808773647
1103	30-31	0.34888062479751625
1103	32-33	0.3621161963792403
1103	34-35	0.3696961126271461
1103	36-37	0.32238656518000397
1103	38-39	0.32745913575610786
1103	40-41	0.3343090770785295
1103	42-43	0.38963321148232666
1103	44-45	0.31803973481797954
1103	46-47	0.36462322733478914
1103	48-49	0.33598344581537987
1103	50-51	0.3247942954955505
1103	52-53	0.3370033591088841
1103	54-55	0.315622919517331
1103	56-57	0.331140566117881
1103	58-59	0.2821593222029861
1103	60-61	0.27022837458876836
1103	62-63	0.24628039960457926
1103	64-65	0.2974798837736401
1103	66-67	0.26662270780632014
1103	68-69	0.2833818514434938
1103	70-71	0.2829038901817782
1103	72-73	0.269215952374978
1103	74-75	0.18606786692749466
1103	76-77	0.19835155905010993
1103	78-79	0.18694915146472368
1103	80-81	0.24190278234274132
1103	82-83	0.24922665784951903
1103	84-85	0.19378225328381404
1103	86-87	0.20021440369774268
1103	88-89	0.23426262329179792
1103	90-91	0.16838871685774137
1103	92-93	0.179681047182072
1103	94-95	0.17786018667833758
1103	96-97	0.20015076461710635
1103	98-99	0.23552546120637885
1103	100	0.23907492681323816
1104	1	0.1367901999027552
1104	2	0.11953888716378458
1104	3	0.1606329498588508
1104	4	0.08887043286397756
1104	5	0.05834731132800641
1104	6	0.053733727025750966
1104	7	0.10643109265814843
1104	8	0.0633877365311335
1104	9	0.09228763298709453
1104	10-11	0.11942499778470506
1104	12-13	0.13337116945834282
1104	14-15	0.20403976189160744
1104	16-17	0.23079573628903205
1104	18-19	0.18393985599725227
1104	20-21	0.20273350442888471
1104	22-23	0.20568275273953418
1104	24-25	0.19812715711567108
1104	26-27	0.17948464974866596
1104	28-29	0.19815376569723497
1104	30-31	0.2072510736860238
1104	32-33	0.17956344269858704
1104	34-35	0.23268770721947618
1104	36-37	0.26011723858601954
1104	38-39	0.2920274944102701
1104	40-41	0.2176500120335021
1104	42-43	0.16161766011624934
1104	44-45	0.19207636085258883
1104	46-47	0.22351982129826808
1104	48-49	0.15325370077301415
1104	50-51	0.2172225052056831
1104	52-53	0.22098698648553494
1104	54-55	0.17933094388897075
1104	56-57	0.18467950893794693
1104	58-59	0.23047925530437396
1104	60-61	0.2027311832821681
1104	62-63	0.19204654594268078
1104	64-65	0.21314257752139554
1104	66-67	0.18093523201241624
1104	68-69	0.16747540662480986
1104	70-71	0.11060752004563312
1104	72-73	0.10453449721732966
1104	74-75	0.1504377478375929
1104	76-77	0.15848894818769566
1104	78-79	0.13860674447553123
1104	80-81	0.1624216697952292
1104	82-83	0.14972821295356908
1104	84-85	0.12923558719446504
1104	86-87	0.12098597317704929
1104	88-89	0.17258927251542389
1104	90-91	0.13327915421305647
1104	92-93	0.14181916577149423
1104	94-95	0.09761370314727458
1104	96-97	0.08574836656320883
1104	98-99	0.07861925501675415
1104	100	0.10976173902518838
1105	1	0.19326001796005698
1105	2	0.19324426386525317
1105	3	0.18045337397217764
1105	4	0.12911426529338854
1105	5	0.11204946513077374
1105	6	0.09788592841663046
1105	7	0.15412314468782284
1105	8	0.1464936037174951
1105	9	0.09629943685356324
1105	10-11	0.14193911055593844
1105	12-13	0.14951758977468899
1105	14-15	0.25256100532621417
1105	16-17	0.20584324886588945
1105	18-19	0.2051933621585249
1105	20-21	0.22363143422005294
1105	22-23	0.21692487803979077
1105	24-25	0.17792079088359003
1105	26-27	0.20199075563095903
1105	28-29	0.2602497073627674
1105	30-31	0.2204603757773782
1105	32-33	0.24729346450350675
1105	34-35	0.24062051985324473
1105	36-37	0.2600241270284016
1105	38-39	0.27701870697554654
1105	40-41	0.23843564394825023
1105	42-43	0.2008418983287541
1105	44-45	0.1987603505997484
1105	46-47	0.20708152721883266
1105	48-49	0.1880792865600256
1105	50-51	0.17820538859214707
1105	52-53	0.16106050886201473
1105	54-55	0.12851278084050932
1105	56-57	0.15109423557066748
1105	58-59	0.11532548778616558
1105	60-61	0.1080055577124881
1105	62-63	0.1256692351494948
1105	64-65	0.11577593754436322
1105	66-67	0.08418361175044708
1105	68-69	0.18077600739326982
1105	70-71	0.11465553116463667
1105	72-73	0.09418858954330744
1105	74-75	0.07966979150787523
1105	76-77	0.03623226444973682
1105	78-79	0.09685570332817406
1105	80-81	0.08931771620496676
1105	82-83	0.08367339346106917
1105	84-85	0.08329271307383834
1105	86-87	0.058469846094126865
1105	88-89	0.10534716735828908
1105	90-91	0.11560069878725443
1105	92-93	0.05661866622219236
1105	94-95	0.11002654639857212
1105	96-97	0.08996134980917248
1105	98-99	0.11283057645558614
1105	100	0.13808850066664746
1106	1	0.11004393946722502
1106	2	0.0647586987007216
1106	3	0.09963748164337005
1106	4	0.06716562186873176
1106	5	0.04767204606700659
1106	6	0.05021891506696363
1106	7	0.03951962181862001
1106	8	0.0693265262552174
1106	9	0.07180281582557768
1106	10-11	0.0685392425804281
1106	12-13	0.10041768333160661
1106	14-15	0.13405763110328195
1106	16-17	0.09385238610011726
1106	18-19	0.12190158329479317
1106	20-21	0.08997340613397853
1106	22-23	0.10036411590707672
1106	24-25	0.08562655674434438
1106	26-27	0.09857939487642398
1106	28-29	0.15071996148616051
1106	30-31	0.14543404211636357
1106	32-33	0.12043607469409068
1106	34-35	0.08238979044170236
1106	36-37	0.12641947862670833
1106	38-39	0.13660444453176268
1106	40-41	0.1020084260081191
1106	42-43	0.06896924021992845
1106	44-45	0.07456520131234612
1106	46-47	0.11835632336448043
1106	48-49	0.07534799438223416
1106	50-51	0.05342819178542868
1106	52-53	0.07208512335527217
1106	54-55	0.0888705766964506
1106	56-57	0.09794056001847196
1106	58-59	0.10513994114305092
1106	60-61	0.12268953003484029
1106	62-63	0.11924952075930406
1106	64-65	0.1474894064164971
1106	66-67	0.10123671123897537
1106	68-69	0.12503099511689442
1106	70-71	0.10450175588171362
1106	72-73	0.04344483577372671
1106	74-75	0.06608594408638879
1106	76-77	0.0750680269386379
1106	78-79	0.021840340047134532
1106	80-81	0.033909841276795305
1106	82-83	0.05602328684418012
1106	84-85	0.06466524533198026
1106	86-87	0.054186031441304294
1106	88-89	0.030124126710887822
1106	90-91	9.607504572670678E-4
1106	92-93	-0.014706897612597913
1106	94-95	0.01770253551367773
1106	96-97	-0.011234611571296682
1106	98-99	-0.03297550901839941
1106	100	-0.01704169542295375
1107	1	0.03455175870880822
1107	2	0.03934204281370768
1107	3	0.07053299065812269
1107	4	0.03644326502057993
1107	5	0.03873927363054008
1107	6	0.07609278128411745
1107	7	0.08975410457217237
1107	8	0.0727390137638082
1107	9	0.08946469400071777
1107	10-11	0.08109832197759204
1107	12-13	0.09067640609187322
1107	14-15	0.1474691748950221
1107	16-17	0.10454843508238554
1107	18-19	0.10518959925883564
1107	20-21	0.09889529188210133
1107	22-23	0.13983200026818565
1107	24-25	0.13343679418240129
1107	26-27	0.10187824493021935
1107	28-29	0.1432081618144423
1107	30-31	0.1564836179544642
1107	32-33	0.11687427747798296
1107	34-35	0.1410980004664637
1107	36-37	0.11692867167050025
1107	38-39	0.14470264221603912
1107	40-41	0.11426189832654643
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2216	24-25	-0.41549083386480135
2216	26-27	-0.37255056171674994
2216	28-29	-0.38421969425142066
2216	30-31	-0.41388092593648196
2216	32-33	-0.4015156021553423
2216	34-35	-0.4328106716419384
2216	36-37	-0.4499401838117265
2216	38-39	-0.3913687585811161
2216	40-41	-0.35917220522412663
2216	42-43	-0.424339161255773
2216	44-45	-0.44750560081805446
2216	46-47	-0.41380945595523144
2216	48-49	-0.40251086704260075
2216	50-51	-0.3951304363868502
2216	52-53	-0.361133751558917
2216	54-55	-0.4085659844757856
2216	56-57	-0.32806239122966474
2216	58-59	-0.27498948553856906
2216	60-61	-0.3069631501493859
2216	62-63	-0.27506707057053603
2216	64-65	-0.2818968409825402
2216	66-67	-0.2629410291594354
2216	68-69	-0.3277364801485021
2216	70-71	-0.2990228706645226
2216	72-73	-0.3044987528967411
2216	74-75	-0.3204078647818207
2216	76-77	-0.2908808220641035
2216	78-79	-0.3530494919972966
2216	80-81	-0.34137918731460104
2216	82-83	-0.28929677030355805
2216	84-85	-0.28695378885107914
2216	86-87	-0.23353421870820767
2216	88-89	-0.23046183578802726
2216	90-91	-0.2180711075092745
2216	92-93	-0.2685783203522334
2216	94-95	-0.3173500682855348
2216	96-97	-0.2926580049034584
2216	98-99	-0.27278774251414717
2216	100	-0.33366356593356983
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	13.0
8	77.0
9	137.0
10	232.0
11	234.0
12	165.0
13	203.0
14	356.0
15	574.0
16	1210.0
17	2183.0
18	3856.0
19	6453.0
20	9504.0
21	14096.0
22	20783.0
23	26140.0
24	26285.0
25	23451.0
26	20067.0
27	20629.0
28	24465.0
29	30182.0
30	36864.0
31	47405.0
32	61521.0
33	81683.0
34	118923.0
35	190995.0
36	349361.0
37	882323.0
38	1367380.0
39	513663.0
40	2277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.49426159307549	19.751990610488214	13.754100057628696	23.999647738807596
2	15.151568258098369	20.99665140344416	39.86904572600991	23.98273461244756
3	17.558192230049848	26.291069295101554	31.021016149631276	25.12972232521732
4	12.172492610766406	18.040338430632097	39.3106969632754	30.476471995326094
5	12.818162803325595	38.1403568966743	35.44534291981829	13.596137380181816
6	29.33814857731428	38.93302433371959	18.223561220548472	13.505265868417665
7	25.703493327222304	33.40802175250857	23.153933501386568	17.734551418882557
8	25.55962871402488	34.897421035813615	20.808092095895372	18.734858154266135
9	26.111938359668674	14.941739700712544	20.82987549730398	38.116446442314796
10-11	24.0594578713909	26.220494884891714	29.178106600138886	20.541940643578492
12-13	25.387936372898874	24.160070922223216	28.73365826477956	21.71833444009835
14-15	22.55871513864079	25.186823090168815	26.84343166127553	25.41103010991487
16-17	21.63297749486249	27.981873429168285	27.552603953301123	22.8325451226681
18-19	21.516310128689433	26.922558978044343	30.000005149740282	21.56112574352594
20-21	22.504596786922544	26.135086442253	29.77498724795562	21.585329522868836
22-23	22.377997086516803	26.108929253083584	29.472270733784494	22.040802926615115
24-25	22.092269956595416	26.291278065118977	29.72516351069125	21.891288467594357
26-27	22.002677349974547	26.374073014562693	29.472491503572247	22.150758131890512
28-29	21.79625562287412	26.53591043569389	29.671703972253194	21.996129969178796
30-31	22.224033215854778	26.148666698852573	29.555055601152258	22.072244484140395
32-33	22.25006566038902	26.388485485191648	29.248252918668666	22.113195935750664
34-35	22.3500292574027	26.547517047506425	29.32329462172335	21.779159073367524
36-37	22.36108920661671	26.236062572539637	29.308479582042935	22.094368638800717
38-39	22.34255269486372	26.3909926122457	29.249329851906314	22.017124840984263
40-41	22.336533991343405	26.181945569716568	29.334667309192934	22.14685312974709
42-43	22.36417147205024	26.286523168192932	29.0588529134207	22.29045244633613
44-45	22.32619188318251	26.60427714542835	28.848935219695825	22.220595751693313
46-47	22.581962918131453	26.531673463060905	28.346319737712484	22.54004388109516
48-49	22.811136234862715	26.642749393002408	28.088638964565554	22.45747540756932
50-51	22.686311404019797	26.869049174713748	27.811963094020385	22.632676327246067
52-53	22.505459752837957	27.028785961853117	27.80392087693872	22.6618334083702
54-55	22.48503782675319	26.939803951306345	27.79763607576114	22.777522146179333
56-57	22.704473187729793	26.896559448827922	27.630302001904912	22.768665361537376
58-59	22.445739637809346	26.853027739797458	28.007861282988163	22.693371339405036
60-61	22.566596474314903	26.97717898843706	27.905978812518445	22.550245724729596
62-63	22.622748880209485	26.805179817287883	27.75108110268934	22.82099019981329
64-65	22.277422630877197	26.8137512147466	27.93238670218765	22.97643945218855
66-67	22.487369091359945	26.837935290299107	27.77708110329548	22.897614515045465
68-69	22.455824262920167	26.718971024331722	27.869927822509887	22.955276890238228
70-71	22.479960057873853	26.55985306488613	27.7992769115611	23.16090996567892
72-73	22.601244288983423	26.596052584483648	27.830608087595145	22.972095038937784
74-75	22.46674471370372	26.882144467872287	27.685055614629427	22.966055203794564
76-77	22.537140314549873	26.73234884736245	27.486800412869723	23.243710425217962
78-79	22.495722129404314	26.824395455872114	27.481921321684666	23.197961093038906
80-81	22.44126075974138	26.936804945811843	27.521455110989805	23.100479183456972
82-83	22.648025083406655	26.638309563370644	27.82729692407954	22.88636842914316
84-85	22.810801026292932	26.746095494577126	27.368738439624313	23.074365039505633
86-87	22.393100684284878	26.873852814443584	27.609647990654246	23.12339851061729
88-89	22.639365241214517	26.686621966794384	27.40806400115354	23.265948790837555
90-91	22.534465280579738	26.72142299683471	27.499613125761037	23.244498596824513
92-93	22.70788278470146	26.61867280378382	27.496909512111028	23.176534899403688
94-95	22.316412402531512	26.57702427922304	27.704753030042813	23.40181028820264
96-97	22.450901820377492	26.565757025083858	27.588667685032657	23.39467346950599
98-99	22.345489297088854	26.68173062389811	27.476315529952007	23.496464549061027
100	22.54280755781917	26.392964368662554	27.425006308482203	23.639221765036076
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	282.0
1	208.0
2	367.5
3	563.5
4	342.0
5	245.5
6	600.5
7	1677.0
8	3305.5
9	4016.0
10	2891.5
11	1768.5
12	1755.0
13	2049.5
14	2438.5
15	2852.5
16	3348.0
17	3854.5
18	4519.0
19	5250.0
20	6096.0
21	7509.0
22	9279.0
23	11280.0
24	13791.0
25	17219.0
26	21955.5
27	26871.0
28	31593.0
29	38144.0
30	45185.5
31	51536.5
32	60322.5
33	68866.0
34	76098.5
35	83940.5
36	93234.0
37	103087.5
38	107493.5
39	110318.0
40	113509.5
41	115124.5
42	118621.0
43	125335.0
44	132286.0
45	142631.0
46	152287.0
47	155834.0
48	157761.5
49	155356.5
50	150105.0
51	147704.5
52	144623.0
53	139722.0
54	135089.0
55	125415.0
56	113890.0
57	102515.5
58	91134.5
59	80177.0
60	65108.0
61	50273.0
62	39289.0
63	30527.5
64	22562.5
65	15263.5
66	10369.5
67	7378.0
68	5143.5
69	3584.0
70	2642.5
71	1927.5
72	1451.0
73	1051.0
74	716.5
75	482.0
76	346.0
77	211.0
78	124.5
79	62.0
80	20.5
81	9.0
82	3.5
83	1.5
84	2.0
85	1.5
86	2.0
87	2.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005381478598580577
2	0.09161387968301288
3	2.8323571571476725E-4
4	0.0
5	0.0401422255272111
6	0.004918001972865503
7	2.57487014286152E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	9.012045500015321E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.050944835724573E-4
24-25	0.0
26-27	0.0
28-29	2.57487014286152E-5
30-31	9.012045500015321E-5
32-33	0.0018281578014316792
34-35	0.0015062990335739893
36-37	9.913250050016852E-4
38-39	8.883301992872244E-4
40-41	0.00160929383928845
42-43	6.694662371439953E-4
44-45	6.694662371439953E-4
46-47	3.6048182000061283E-4
48-49	6.823405878583029E-4
50-51	9.913250050016852E-4
52-53	0.0011458172135733766
54-55	0.0016479168914313729
56-57	0.001029948057144608
58-59	0.0026006188442901355
60-61	0.0019826500100033704
62-63	0.0010428224078589158
64-65	0.004248535735721508
66-67	0.004171289631435663
68-69	0.0035919438492918202
70-71	0.0018539065028602944
72-73	0.004840755868579658
74-75	0.00348894904357736
76-77	0.003643441252149051
78-79	0.0010685711092875308
80-81	2.57487014286152E-5
82-83	2.059896114289216E-4
84-85	1.158691564287684E-4
86-87	3.8623052142922805E-5
88-89	2.8323571571476725E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	4.7635097642938126E-4
98-99	7.209636400012257E-4
100	7.982097442870711E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3883691.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.245845362154874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.84482972292311	34.255198072404916
2	16.54793750373244	16.960260927577522
3	6.915006511618447	10.630960631180791
4	3.4410339457734884	7.0535477388413526
5	1.9997160911138532	5.123857078671667
6	1.1648582607695515	3.581648778013507
7	0.785037962342243	2.816095381512822
8	0.5020339627445278	2.0581723857084717
9	0.3635115726812216	1.6765612056878048
>10	1.3841093905863564	11.109425086074607
>50	0.03354644353695599	1.1763974184276131
>100	0.016525044807730496	1.4728743904322477
>500	0.0012357249133519103	0.4626851993061921
>1k	3.530642609576748E-4	0.2574644788787533
>5k	8.82660652394187E-5	0.2885393378017511
>10k+	1.765321304788374E-4	1.0763118894799604
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12634	0.32530909384912443	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11161	0.28738125664477426	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6379	0.16425096641313638	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	2.57487014286152E-5
6	0.0	0.0	0.0	0.0	2.57487014286152E-5
7	0.0	0.0	0.0	2.57487014286152E-5	5.14974028572304E-5
8	0.0	0.0	0.0	1.28743507143076E-4	5.14974028572304E-5
9	0.0	0.0	0.0	1.8024091000030641E-4	5.14974028572304E-5
10-11	0.0	0.0	0.0	1.8024091000030641E-4	5.14974028572304E-5
12-13	0.0	0.0	0.0	2.188639621432292E-4	2.7036136500045965E-4
14-15	0.0	0.0	0.0	2.446126635718444E-4	3.3473311857199763E-4
16-17	0.0	0.0	0.0	2.832357157147672E-4	3.86230521429228E-4
18-19	0.0	0.0	0.0	3.2185876785769004E-4	4.119792228578432E-4
20-21	0.0	0.0	0.0	3.6048182000061283E-4	4.892253271436888E-4
22-23	0.0	0.0	0.0	4.763509764293812E-4	4.892253271436888E-4
24-25	0.0	0.0	0.0	7.853353935727636E-4	4.892253271436888E-4
26-27	0.0	0.0	0.0	0.0011715659150019916	5.407227300009192E-4
28-29	0.0	0.0	0.0	0.003192838977148285	5.535970807152268E-4
30-31	0.0	0.0	0.0	0.009642888685016393	5.664714314295344E-4
32-33	0.0	0.0	0.0	0.02276185206289584	5.664714314295344E-4
34-35	0.0	0.0	0.0	0.04285871352793	6.179688342867649E-4
36-37	0.0	0.0	0.0	0.07557243869298562	6.179688342867649E-4
38-39	0.0	0.0	0.0	0.11969283859091776	6.694662371439953E-4
40-41	0.0	0.0	0.0	0.1654997784324242	6.694662371439953E-4
42-43	0.0	0.0	0.0	0.21066300073821526	6.952149385726105E-4
44-45	0.0	0.0	0.0	0.2681341023268844	7.338379907155333E-4
46-47	0.0	0.0	0.0	0.33729511436414483	8.110840950013788E-4
48-49	0.0	0.0	0.0	0.41000944719855414	8.625814978586092E-4
50-51	0.0	0.0	0.0	0.4861869803751122	9.01204550001532E-4
52-53	0.0	0.0	0.0	0.559545030745237	9.269532514301472E-4
54-55	0.0	0.0	0.0	0.6397651100460875	9.527019528587624E-4
56-57	0.0	1.28743507143076E-5	0.0	0.7328852887626744	9.527019528587624E-4
58-59	0.0	2.57487014286152E-5	0.0	0.8360860840885642	9.527019528587624E-4
60-61	0.0	2.57487014286152E-5	0.0	0.9430461898230318	9.784506542873776E-4
62-63	0.0	2.57487014286152E-5	0.0	1.0459894981346354	0.001029948057144608
64-65	0.0	2.57487014286152E-5	0.0	1.1531555934805318	0.001029948057144608
66-67	0.0	2.57487014286152E-5	0.0	1.2730415473321641	0.0010556967585732232
68-69	0.0	2.57487014286152E-5	0.0	1.4059434697559614	0.0010814454600018384
70-71	0.0	5.14974028572304E-5	0.0	1.5465828769590577	0.0010814454600018384
72-73	0.0	5.14974028572304E-5	0.0	1.6900417669685872	0.0011458172135733764
74-75	0.0	5.14974028572304E-5	0.0	1.8316853735273995	0.0012101889671449146
76-77	0.0	5.14974028572304E-5	0.0	1.9864865665162341	0.0012101889671449146
78-79	0.0	5.14974028572304E-5	0.0	2.1548701995086637	0.0012488120192878374
80-81	0.0	5.14974028572304E-5	0.0	2.3384455663439754	0.0013131837728593753
82-83	0.0	5.14974028572304E-5	0.0	2.525805992289294	0.0014161785785738361
84-85	0.0	5.14974028572304E-5	0.0	2.7093169873710345	0.0014161785785738361
86-87	0.0	5.14974028572304E-5	0.0	2.9010933156113605	0.001544922085716912
88	0.0	5.14974028572304E-5	0.0	3.0557013933394805	0.001673665592859988
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6620	0.0	58.7067	1
GTATCAA	12415	0.0	43.734554	1
CAACGCA	15390	0.0	34.88919	5
ATCAACG	15600	0.0	34.329113	3
TATCAAC	16060	0.0	33.600822	2
TCAACGC	16180	0.0	33.185707	4
AACGCAG	16625	0.0	32.567654	6
ACGCAGA	18320	0.0	29.347303	7
CGCAGAG	18730	0.0	28.65471	8
GCAGAGT	20865	0.0	25.587486	9
AGAGTAC	19035	0.0	22.603369	10-11
GAGTACT	14350	0.0	22.597733	12-13
CAGAGTA	20190	0.0	22.47417	10-11
GTACATG	11620	0.0	22.432066	1
GTGGTAT	2380	0.0	21.746098	1
TACATGG	11590	0.0	21.676535	2
GTACTAG	1470	0.0	21.124783	1
ACATGGG	11875	0.0	20.945248	3
GTACTTT	15755	0.0	20.343874	14-15
AGTACTT	15420	0.0	20.28296	12-13
>>END_MODULE