FastQCFastQC Report
Mon 16 Jan 2017
ERR1146535_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146535_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5014926
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60302712.024644032633782No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1419782.831108574682857No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1365312.722492814450303No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1058052.109801819608106No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA991391.9768786219377912No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA961661.9175955936338842No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG892781.7802456108026319No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC880981.75671585183909No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC773401.542196235796899No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG539971.076725758266423No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT448770.8948686381414204No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT283280.5648737389145921No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA259050.5165579711445393No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT133250.2657068120247437No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT129320.25787020586146236No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT95170.18977348818307588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGT3350.035.0710832
GTACTAG5350.032.5188981
GTACGCA97950.032.1937873
AGTACGC99450.031.802722
GAGTACG100450.031.690321
TACGCAG100550.031.3610124
CGCAGTG101950.031.114126
GGGCACC5400.030.46535394
AGTAAGG16700.029.55321594
GCTTAGG4650.029.3246541
ACGCAGT111400.028.3909235
TTAGGTA4150.028.310393
TCTCGGG7150.027.61055894
GCATCGT5750.026.970118
GTACCCG4000.025.8613131
AGTAAGA18600.024.76538394
GTGATCG3650.024.4623038
TAGTACT7900.024.3896964
GCTATTG7150.023.6618
CGTTAAC1201.8337998E-423.5102861
Produced by FastQC (version 0.11.5)