##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146535_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5014926
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06137139411429	33.0	31.0	34.0	30.0	34.0
2	32.15683920360939	33.0	31.0	34.0	30.0	34.0
3	32.3560166191884	34.0	31.0	34.0	31.0	34.0
4	35.696931918835894	37.0	35.0	37.0	35.0	37.0
5	35.80537499456622	37.0	35.0	37.0	35.0	37.0
6	36.00685713009524	37.0	37.0	37.0	35.0	37.0
7	36.02580596403616	37.0	37.0	37.0	35.0	37.0
8	36.00793371627019	37.0	37.0	37.0	35.0	37.0
9	37.70492844759823	39.0	38.0	39.0	35.0	39.0
10-11	37.67976366949382	39.0	38.0	39.0	35.0	39.0
12-13	37.69731427741905	39.0	38.0	39.0	35.0	39.0
14-15	38.88041368905543	40.0	38.5	41.0	36.0	41.0
16-17	39.11576890665984	40.5	39.0	41.0	36.5	41.0
18-19	39.265254562081275	41.0	40.0	41.0	36.5	41.0
20-21	39.230534608087936	41.0	39.5	41.0	36.5	41.0
22-23	39.15627668284637	41.0	39.0	41.0	36.0	41.0
24-25	39.02941110197837	40.5	39.0	41.0	36.0	41.0
26-27	38.740754699072326	40.0	38.5	41.0	35.0	41.0
28-29	38.74846049572815	40.0	38.5	41.0	35.5	41.0
30-31	38.86223585353004	40.0	39.0	41.0	35.5	41.0
32-33	38.66916490891391	40.0	38.0	41.0	35.0	41.0
34-35	38.194322009935945	40.0	38.0	41.0	34.0	41.0
36-37	37.942730161920636	40.0	37.5	41.0	33.5	41.0
38-39	37.744142585553604	39.5	37.5	41.0	33.0	41.0
40-41	38.020305683473694	40.0	38.0	41.0	33.0	41.0
42-43	37.754829682432	40.0	37.5	41.0	33.0	41.0
44-45	37.43972293908225	40.0	37.0	41.0	31.0	41.0
46-47	37.04775593897098	39.0	36.0	41.0	31.0	41.0
48-49	36.67459938591317	39.0	35.5	40.0	29.5	41.0
50-51	36.242194500975685	38.5	35.0	40.0	28.5	41.0
52-53	36.370403571259075	38.5	35.0	40.0	28.5	41.0
54-55	36.04186642036194	38.5	34.5	40.0	28.0	41.0
56-57	35.14344787939044	38.0	33.0	40.0	26.5	41.0
58-59	34.763473578672944	38.0	32.5	40.0	26.0	41.0
60-61	33.91677304909385	36.5	31.0	39.0	23.0	41.0
62-63	34.25608732810813	36.5	31.5	39.0	25.5	41.0
64-65	34.03645796568085	36.0	32.0	39.0	26.0	40.5
66-67	33.08083150180082	36.0	30.5	38.5	22.5	39.0
68-69	32.4730250256933	35.0	30.0	37.0	22.0	39.0
70-71	31.704225745305116	34.0	29.5	36.5	21.5	38.0
72-73	31.239699050394762	34.0	29.5	36.0	21.0	37.0
74-75	31.28904045642947	34.0	29.0	35.0	21.5	37.0
76-77	31.060349943349117	34.0	29.0	35.0	21.0	36.5
78-79	30.493687244836714	34.0	29.0	35.0	20.0	36.0
80-81	29.75503068240688	33.0	27.0	35.0	19.0	36.0
82-83	29.345855352601415	32.5	27.0	35.0	18.0	35.0
84-85	29.430167763193317	32.5	26.5	35.0	18.5	35.0
86-87	30.00691794854002	33.5	29.0	35.0	20.0	35.0
88-89	29.633531780927576	33.5	28.0	35.0	18.5	35.0
90-91	29.076078689894928	33.0	26.0	35.0	18.0	35.0
92-93	28.97284576083476	32.5	25.5	35.0	17.5	35.0
94-95	28.916338247064864	32.5	26.0	35.0	16.5	35.0
96-97	28.687832382770953	32.0	25.0	34.5	14.5	35.0
98-99	29.13647329990512	33.0	27.0	35.0	17.5	35.0
100	27.41481748683829	31.0	25.0	34.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1619735002156517
1101	2	0.1671709294572139
1101	3	0.2516412459844659
1101	4	0.22814861590305213
1101	5	0.1695287238551373
1101	6	0.1340927100643725
1101	7	0.1474026825000223
1101	8	0.19803545574335146
1101	9	0.27592597464314395
1101	10-11	0.2739779558666413
1101	12-13	0.25022445933289106
1101	14-15	0.38259645103941864
1101	16-17	0.363412667584349
1101	18-19	0.30548672980279434
1101	20-21	0.28868693395858713
1101	22-23	0.33005276018996454
1101	24-25	0.36046766516491147
1101	26-27	0.3630979631160045
1101	28-29	0.41201416265869284
1101	30-31	0.37565103667756716
1101	32-33	0.3675827178427653
1101	34-35	0.43668043387857125
1101	36-37	0.49843248249488425
1101	38-39	0.5054852843153128
1101	40-41	0.473828946432846
1101	42-43	0.4902024500610409
1101	44-45	0.5172684229482414
1101	46-47	0.6391101841003888
1101	48-49	0.6632439221339652
1101	50-51	0.7130128295742395
1101	52-53	0.6103056517066747
1101	54-55	0.7567396599468879
1101	56-57	0.7728661089267206
1101	58-59	0.7923954980752299
1101	60-61	0.8371484654598902
1101	62-63	0.7739594057403423
1101	64-65	0.7184604851993228
1101	66-67	0.71951775932434
1101	68-69	0.6441726748979377
1101	70-71	0.6803904871573572
1101	72-73	0.5866299608180476
1101	74-75	0.7122295901774471
1101	76-77	0.6023388102801341
1101	78-79	0.5780410921373687
1101	80-81	0.5982913431954273
1101	82-83	0.5797796711197485
1101	84-85	0.5985968462188573
1101	86-87	0.5811887746713609
1101	88-89	0.4657922077680361
1101	90-91	0.48992012983308797
1101	92-93	0.5702787405782779
1101	94-95	0.5208179537796944
1101	96-97	0.5271357730389354
1101	98-99	0.4719768723700888
1101	100	0.4329562977569843
1102	1	0.1711254216961393
1102	2	0.23178646628237942
1102	3	0.27475174708505534
1102	4	0.21089105319821755
1102	5	0.19522659568832523
1102	6	0.16172102239372066
1102	7	0.17395563981748552
1102	8	0.20161319176455095
1102	9	0.2827694838028094
1102	10-11	0.2887323927141807
1102	12-13	0.2537950165606091
1102	14-15	0.3406700625162813
1102	16-17	0.3265930467268632
1102	18-19	0.2597985892800807
1102	20-21	0.2874387485302705
1102	22-23	0.30543319707618366
1102	24-25	0.36872006712629
1102	26-27	0.40258349764165047
1102	28-29	0.4079251013394938
1102	30-31	0.4285148628758648
1102	32-33	0.41094499348589153
1102	34-35	0.49724275198315837
1102	36-37	0.5406525979673873
1102	38-39	0.5039964744619212
1102	40-41	0.49204639987001997
1102	42-43	0.4582300173471978
1102	44-45	0.5351561013621264
1102	46-47	0.5872978135674884
1102	48-49	0.6029472835995193
1102	50-51	0.6047810111016076
1102	52-53	0.5797807411519997
1102	54-55	0.6218672746233551
1102	56-57	0.7635964500961236
1102	58-59	0.7451406468738924
1102	60-61	0.8214969362702789
1102	62-63	0.7388616807860444
1102	64-65	0.6019118610992038
1102	66-67	0.7274164227049127
1102	68-69	0.78633111182058
1102	70-71	0.693501725143765
1102	72-73	0.6346602372961172
1102	74-75	0.5500807689854277
1102	76-77	0.6121688259535354
1102	78-79	0.5611606793559716
1102	80-81	0.7219819550210076
1102	82-83	0.7601695240402648
1102	84-85	0.7853478678613435
1102	86-87	0.682584981941389
1102	88-89	0.6415931421618559
1102	90-91	0.7237330099961135
1102	92-93	0.7273319026133542
1102	94-95	0.6555990566987049
1102	96-97	0.672718404560662
1102	98-99	0.620161232211391
1102	100	0.5505449351164167
1103	1	0.17963197391993901
1103	2	0.2081220027116757
1103	3	0.2513385479600956
1103	4	0.16359434646354742
1103	5	0.17345189431711105
1103	6	0.17826156087584621
1103	7	0.15342916566832088
1103	8	0.18687847824398318
1103	9	0.26929850343251616
1103	10-11	0.2724491272249878
1103	12-13	0.25598715576187914
1103	14-15	0.4121087812067117
1103	16-17	0.34379433157544526
1103	18-19	0.28587650497321704
1103	20-21	0.29328215758155807
1103	22-23	0.32796382384974976
1103	24-25	0.34110998330192643
1103	26-27	0.3469301787288259
1103	28-29	0.3987588174754322
1103	30-31	0.36094977588703614
1103	32-33	0.3585471214009033
1103	34-35	0.3916425009695459
1103	36-37	0.49536732936309136
1103	38-39	0.4634696103842728
1103	40-41	0.4215167960316677
1103	42-43	0.473216029517765
1103	44-45	0.4610976446968067
1103	46-47	0.5502935201355896
1103	48-49	0.5921411452129846
1103	50-51	0.5939181848657142
1103	52-53	0.5951850485392427
1103	54-55	0.6903111486928708
1103	56-57	0.7317132630942851
1103	58-59	0.7633113486733407
1103	60-61	0.7446301165737879
1103	62-63	0.6409670551977769
1103	64-65	0.7063465451699145
1103	66-67	0.7051485758090834
1103	68-69	0.7237580672121879
1103	70-71	0.6844237794649111
1103	72-73	0.6885260077201352
1103	74-75	0.6104779670185856
1103	76-77	0.6328563337278759
1103	78-79	0.6669254984342814
1103	80-81	0.799864173586414
1103	82-83	0.705728016221002
1103	84-85	0.7064369107681827
1103	86-87	0.6220530191496891
1103	88-89	0.6280920392457041
1103	90-91	0.7147464694838561
1103	92-93	0.7311577395714828
1103	94-95	0.6580078228384103
1103	96-97	0.6015321474648445
1103	98-99	0.6033700563615092
1103	100	0.6059630313783586
1104	1	0.14274877225827964
1104	2	0.11556104429025282
1104	3	0.22947674758177072
1104	4	0.15610411983990957
1104	5	0.15831892447177864
1104	6	0.1574759817059359
1104	7	0.10770207839411228
1104	8	0.15666031126205127
1104	9	0.20657887137139141
1104	10-11	0.18276669704806636
1104	12-13	0.17022624184919977
1104	14-15	0.29186368644019467
1104	16-17	0.2562652339900424
1104	18-19	0.18538690888998133
1104	20-21	0.17837187977287527
1104	22-23	0.23990744611894854
1104	24-25	0.28726672822978827
1104	26-27	0.2519512091060392
1104	28-29	0.23656453684785106
1104	30-31	0.2444567674674687
1104	32-33	0.30538544342159923
1104	34-35	0.29712256476540233
1104	36-37	0.35278508093016825
1104	38-39	0.3659647700593567
1104	40-41	0.34853567515336437
1104	42-43	0.37783069514588874
1104	44-45	0.37622334478211883
1104	46-47	0.5032176698332975
1104	48-49	0.5433638743402511
1104	50-51	0.46387122815045956
1104	52-53	0.5142665366146062
1104	54-55	0.5689534839178236
1104	56-57	0.5941391375054721
1104	58-59	0.7111429572931982
1104	60-61	0.7108600041892288
1104	62-63	0.6374784605610699
1104	64-65	0.6243144246996053
1104	66-67	0.6116929918173852
1104	68-69	0.6370471885355826
1104	70-71	0.6007015139703036
1104	72-73	0.5651385962739681
1104	74-75	0.4368882633725839
1104	76-77	0.4754173663305252
1104	78-79	0.5286887861461622
1104	80-81	0.5607865429347321
1104	82-83	0.5657547214619036
1104	84-85	0.5893819053900451
1104	86-87	0.5413839045026023
1104	88-89	0.5199846248322899
1104	90-91	0.5711296896908244
1104	92-93	0.5164935950664571
1104	94-95	0.45519353553452646
1104	96-97	0.4805499880493933
1104	98-99	0.32355684468538115
1104	100	0.3917341823000626
1105	1	0.09510914216011201
1105	2	0.1359620131741721
1105	3	0.09927647888319058
1105	4	0.10719207449457002
1105	5	0.06478240009767688
1105	6	0.04111822033772938
1105	7	0.03788912790721355
1105	8	0.060017239076273654
1105	9	0.07970226677998227
1105	10-11	0.09190959761978945
1105	12-13	0.06991096231441674
1105	14-15	0.14864910022619426
1105	16-17	0.15510263212484432
1105	18-19	0.11035111510142315
1105	20-21	0.1127302687581988
1105	22-23	0.15142430842563925
1105	24-25	0.2104265254310036
1105	26-27	0.19907180965716975
1105	28-29	0.19320315687473055
1105	30-31	0.226057034204743
1105	32-33	0.2693730283705591
1105	34-35	0.24916659602857294
1105	36-37	0.31736374390958844
1105	38-39	0.28442852026774545
1105	40-41	0.22985981977988246
1105	42-43	0.23131482329745978
1105	44-45	0.30661892008146197
1105	46-47	0.34808166324331324
1105	48-49	0.38253465484096694
1105	50-51	0.3723411466583002
1105	52-53	0.4294979410059696
1105	54-55	0.5606736647500057
1105	56-57	0.5472612678733171
1105	58-59	0.5172861618669558
1105	60-61	0.4458559639040516
1105	62-63	0.426218450558423
1105	64-65	0.39139074882461955
1105	66-67	0.4602951512125273
1105	68-69	0.48654819370361224
1105	70-71	0.45656235948823465
1105	72-73	0.491301051041404
1105	74-75	0.4739239100633732
1105	76-77	0.43633168678790213
1105	78-79	0.4451995291859845
1105	80-81	0.4481238381010968
1105	82-83	0.46190281948785383
1105	84-85	0.46296275522284347
1105	86-87	0.3377749589974215
1105	88-89	0.3489664337424898
1105	90-91	0.4984294258955053
1105	92-93	0.43660583065823033
1105	94-95	0.3928007256730943
1105	96-97	0.3817963964123905
1105	98-99	0.4268453818471798
1105	100	0.42794359067286436
1106	1	0.15854655096325132
1106	2	0.12401668792936249
1106	3	0.17056148660940806
1106	4	0.14536767510405468
1106	5	0.16818809059206075
1106	6	0.1348443034162088
1106	7	0.10487296711080774
1106	8	0.11532052067573062
1106	9	0.10634172130482966
1106	10-11	0.14694708289376734
1106	12-13	0.17515685121519198
1106	14-15	0.22405260579407127
1106	16-17	0.21180579547301903
1106	18-19	0.20597436989810802
1106	20-21	0.1655632429486502
1106	22-23	0.22822077190226508
1106	24-25	0.25164758694509004
1106	26-27	0.23380012925466076
1106	28-29	0.2467659037986465
1106	30-31	0.22572607465389183
1106	32-33	0.26877544163343714
1106	34-35	0.333262408996589
1106	36-37	0.3439510124634211
1106	38-39	0.3437776587363146
1106	40-41	0.350311648211715
1106	42-43	0.29844600181573355
1106	44-45	0.3940988290760856
1106	46-47	0.35863335797777296
1106	48-49	0.3600355379272955
1106	50-51	0.36133334891102464
1106	52-53	0.4161947518298845
1106	54-55	0.5154827899121415
1106	56-57	0.5175602100055201
1106	58-59	0.6232145831301565
1106	60-61	0.5446606638224623
1106	62-63	0.4412055571814477
1106	64-65	0.4893795038754618
1106	66-67	0.5309979629087636
1106	68-69	0.4446186057535968
1106	70-71	0.3901931990153038
1106	72-73	0.38242556653086623
1106	74-75	0.32931349831725143
1106	76-77	0.3304183001486507
1106	78-79	0.34143490682725286
1106	80-81	0.4057709859221532
1106	82-83	0.4150999560092039
1106	84-85	0.38512945660102815
1106	86-87	0.2697775883157618
1106	88-89	0.38547560454658125
1106	90-91	0.42074805610515753
1106	92-93	0.32414067142099157
1106	94-95	0.24510722689770148
1106	96-97	0.1648717778012383
1106	98-99	0.2071414943049028
1106	100	0.12571481054110478
1107	1	0.1031094762045015
1107	2	0.12096413295935093
1107	3	0.14886250244263266
1107	4	0.11261971387444447
1107	5	0.09048555917409828
1107	6	0.08316663989748463
1107	7	0.04814789882754411
1107	8	0.026704458590288027
1107	9	0.12268312993555242
1107	10-11	0.1402760899994746
1107	12-13	0.08676801924949018
1107	14-15	0.17312487167814083
1107	16-17	0.173176601424764
1107	18-19	0.16245705693056323
1107	20-21	0.12220223128935004
1107	22-23	0.16458712684079302
1107	24-25	0.14060048166594896
1107	26-27	0.17258904393256813
1107	28-29	0.18082296401937725
1107	30-31	0.1623792767599639
1107	32-33	0.18660487409093207
1107	34-35	0.1355964864253565
1107	36-37	0.21335176312237536
1107	38-39	0.1927219506213831
1107	40-41	0.22576850345002697
1107	42-43	0.19730947538644017
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2216	80-81	-3.2115366063683624
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2216	84-85	-2.9359533325967497
2216	86-87	-2.8961792432132754
2216	88-89	-3.0697694919968086
2216	90-91	-3.167734978124848
2216	92-93	-3.0821531693517166
2216	94-95	-3.0002025029933
2216	96-97	-2.9247093743402566
2216	98-99	-2.8820723517199305
2216	100	-3.048463197766292
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	134.0
1	73.0
2	60.0
3	63.0
4	57.0
5	70.0
6	148.0
7	731.0
8	2212.0
9	2686.0
10	2419.0
11	2648.0
12	3509.0
13	4238.0
14	5498.0
15	6282.0
16	7420.0
17	8472.0
18	10147.0
19	12225.0
20	15389.0
21	18780.0
22	23690.0
23	29818.0
24	37221.0
25	46572.0
26	56315.0
27	67102.0
28	81055.0
29	102363.0
30	130401.0
31	168860.0
32	229959.0
33	328551.0
34	487838.0
35	714735.0
36	931384.0
37	962043.0
38	444903.0
39	68715.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	55.64737220160422	15.01209146414462	12.806317091663674	16.534219242587493
2	11.818215901472024	30.635339360975294	26.385983399189623	31.16046133836306
3	40.9579768819903	18.44590704415061	28.757534113532756	11.838581960326335
4	9.640934335321395	12.12594210589397	49.72328864431478	28.509834914469856
5	8.932735207800361	45.66321097206966	34.401690992937844	11.002362827192137
6	24.973286432858306	37.07553946647851	13.856493799939612	24.09468030072357
7	23.749749988882858	34.183214926605906	22.4954768676653	19.571558216845933
8	20.67685748442811	14.928793563652063	20.76089134923808	43.633457602681744
9	13.869474863093645	30.8196194321375	32.28826303569267	23.022642669076184
10-11	28.71245892472621	17.208201534168207	25.911194051578455	28.168145489527124
12-13	40.10559327693622	19.411416840583186	20.556488993130785	19.926500889349807
14-15	25.519295941027554	23.621664724430662	36.88473189116286	13.974307443378924
16-17	18.03091311673624	26.568255564279248	32.78426249306752	22.616568825916985
18-19	15.33854006714884	41.642540121391185	23.03496118880087	19.983958622659102
20-21	23.639737257814648	24.435647418469387	18.14363674198813	33.78097858172784
22-23	17.888210573733378	26.926337659499307	23.948638373915422	31.236813392851893
24-25	31.850616233958522	24.978727689499046	19.64454751846309	23.52610855807934
26-27	26.747375177018075	16.106947384290276	37.20831962771639	19.937357810975257
28-29	17.27570139724571	39.38388696002714	24.312691571268026	19.027720071459118
30-31	21.775666864959433	23.292596567015742	22.862920136540822	32.068816431483995
32-33	24.712421043730906	25.580240768322387	26.848576566055538	22.85876162189117
34-35	32.25364300259715	20.996695676139964	25.119442462904885	21.630218858358
36-37	25.679130040237137	17.470664785277297	36.59496113401908	20.25524404046649
38-39	21.551430305430856	28.307574453046364	30.854952304537754	19.28604293698503
40-41	20.61278564867662	32.11865884843143	25.465812804914716	21.802742697977234
42-43	17.89105808604549	31.467286141585614	16.8299273880801	33.8117283842888
44-45	23.963691117192866	20.374390226145643	21.431767061151813	34.230151595509675
46-47	32.304422317404466	28.67841705373583	23.82533612011832	15.191824508741384
48-49	27.367433375224763	25.276828009338654	25.746962140035322	21.60877647540126
50-51	11.666283020651857	28.423312619108128	39.53675641323822	20.373647947001796
52-53	22.953707178649065	34.252792240956566	19.784401867674074	23.009098712720295
54-55	16.786248636943075	23.13493966842995	27.488702822621224	32.59010887200575
56-57	28.072950874856932	17.42124542124542	25.955979124120717	28.549824579776928
58-59	38.44449923430505	20.229317509170485	20.880073439690825	20.44610981683364
60-61	25.00022441378631	24.123694086307747	36.53588097172566	14.340200528180288
62-63	18.340807295643074	26.22271167646578	32.389585808211706	23.04689521967944
64-65	15.595639158606717	40.65731182381735	23.049798320859832	20.697250696716104
66-67	23.527479747095903	24.33754604111492	18.57061369767865	33.56436051411053
68-69	18.06420165254338	26.722085913863513	24.29384994572546	30.91986248786765
70-71	31.26870908836295	25.041402865944086	19.93696111940742	23.752926926285546
72-73	26.298747379006944	16.705296901924616	36.489758840213256	20.50619687885519
74-75	17.55502061387301	38.75837010018437	24.30409290231463	19.382516383627994
76-77	21.834394721495197	23.388261404998055	22.99951296730777	31.77783090619898
78-79	24.59044287753955	25.395718290248436	26.827296859657285	23.18654197255473
80-81	31.716454607350304	21.212481499960003	25.133472589419103	21.93759130327059
82-83	25.413633560023396	18.111287186226562	35.68850423657474	20.786575017175302
84-85	21.53717216955445	28.215091161588656	30.339738042833442	19.907998626023453
86-87	20.759217492305915	31.849350932236405	25.446580044509897	21.944851530947783
88-89	18.343514944093975	31.314723822914676	17.32114844540153	33.02061278758982
90-91	24.033544200527164	20.648455517820118	21.676837016029072	33.641163265623646
92-93	31.793027627321347	28.415973800218087	23.85059038973632	15.94040818272425
94-95	26.974522753579315	25.44490053615573	25.62797445243644	21.952602257828516
96-97	12.662049497087166	28.23094903909035	38.25926503285447	20.84773643096801
98-99	23.16748758597861	33.7148454731221	20.290578209307146	22.827088731592145
100	21.20511707617531	14.842161784370484	22.010305001228687	41.942416138225525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1006.0
1	677.0
2	351.0
3	344.0
4	290.0
5	211.5
6	188.0
7	197.0
8	199.5
9	207.5
10	181.5
11	160.0
12	174.5
13	202.0
14	246.0
15	272.5
16	290.5
17	335.0
18	410.5
19	524.0
20	652.5
21	810.0
22	1095.0
23	1473.5
24	1974.0
25	2560.0
26	3147.0
27	4080.5
28	5248.0
29	7014.0
30	9124.0
31	10760.0
32	12951.0
33	14857.0
34	16336.0
35	18265.5
36	19902.5
37	22677.0
38	24084.5
39	23639.0
40	23582.5
41	23552.0
42	24825.5
43	27780.0
44	35281.5
45	51632.5
46	106357.0
47	524242.0
48	1433603.5
49	1447765.0
50	604104.5
51	191815.5
52	78118.0
53	47573.0
54	36342.5
55	28566.0
56	24029.0
57	19899.5
58	15925.0
59	14428.0
60	12136.5
61	9047.5
62	6936.5
63	5618.0
64	4170.0
65	2576.5
66	1820.0
67	1456.5
68	1115.5
69	836.5
70	635.5
71	457.5
72	355.5
73	350.5
74	308.5
75	235.5
76	174.0
77	119.5
78	87.0
79	80.5
80	74.0
81	54.0
82	39.0
83	31.5
84	24.0
85	16.5
86	13.5
87	12.5
88	10.5
89	12.0
90	11.5
91	5.5
92	5.0
93	8.0
94	8.5
95	6.0
96	4.0
97	3.0
98	7.0
99	10.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.062333920779688475
2	0.009411903585416815
3	0.011306248586718927
4	0.009870534480468904
5	0.011844641376562685
6	0.012363093692708526
7	0.00297113058098963
8	0.005603273109114671
9	0.007318153847135532
10-11	0.007019046741666777
12-13	0.006131695662109471
14-15	0.01029925466497412
16-17	0.009671129743489734
18-19	0.00785654663697929
20-21	0.0070290169785157345
22-23	0.0026221722912760826
24-25	0.0038086304763021426
26-27	0.005074850556119871
28-29	0.006191517083203222
30-31	0.009072915532552225
32-33	0.010379016559765787
34-35	0.011705058060677266
36-37	0.0179663668018232
38-39	0.01848481911796904
40-41	0.018375146512630496
42-43	0.02427752672721392
44-45	0.027657437019010845
46-47	0.029960561731120258
48-49	0.0312965734688807
50-51	0.03238332928541717
52-53	0.030399252152474434
54-55	0.03664062041992245
56-57	0.03915312010585999
58-59	0.03655088828828182
60-61	0.03718898344661516
62-63	0.032552823311849464
64-65	0.03519493607682347
66-67	0.03747812031523496
68-69	0.03961175100091208
70-71	0.039641661711458956
72-73	0.03584300147200577
74-75	0.038255798789453725
76-77	0.038195977368359973
78-79	0.037059370367578705
80-81	0.041855054291927735
82-83	0.038166066657813094
84-85	0.03469642423437554
86-87	0.03308124586484427
88-89	0.031057287784505696
90-91	0.033101186338542185
92-93	0.03498556110299534
94-95	0.03835550115794331
96-97	0.033529906523047395
98-99	0.03075818067903694
100	0.028674401177604614
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5014926.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.14719042246895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.06342886433094	37.10387584139333
2	14.061257308597622	13.540200664327662
3	4.5958468627193145	6.638313421555601
4	1.9951194118241997	3.8423757694665603
5	0.9884347224304004	2.379517740051837
6	0.4889313191515153	1.412440159601817
7	0.27596341034228977	0.9300804007168915
8	0.1742085886685652	0.6710123273483981
9	0.10639840380962987	0.4610505787982096
>10	0.2481943026969349	1.580883207540393
>50	5.92849470261548E-4	0.020564613052506404
>100	4.4115563243954804E-4	0.05234991069697122
>500	1.9713339546150536E-4	0.07185004185395187
>1k	3.5484009495559117E-4	0.42477895153892653
>5k	3.9426677217287814E-5	0.18065966312713763
>10k+	5.914001582593173E-4	30.69004670892978
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	603027	12.024644032633782	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	141978	2.831108574682857	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	136531	2.722492814450303	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	105805	2.109801819608106	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	99139	1.9768786219377912	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	96166	1.9175955936338842	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	89278	1.7802456108026319	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	88098	1.75671585183909	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	77340	1.542196235796899	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	53997	1.076725758266423	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	44877	0.8948686381414204	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	28328	0.5648737389145921	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	25905	0.5165579711445393	No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	13325	0.2657068120247437	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT	12932	0.25787020586146236	No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	9517	0.18977348818307588	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.9940473697916977E-5	0.0
5	0.0	0.0	0.0	1.9940473697916977E-5	0.0
6	0.0	0.0	0.0	1.9940473697916977E-5	0.0
7	0.0	0.0	0.0	1.9940473697916977E-5	0.0
8	0.0	0.0	0.0	3.9880947395833954E-5	0.0
9	0.0	0.0	0.0	2.1934521067708677E-4	0.0
10-11	0.0	0.0	0.0	9.870534480468905E-4	0.0
12-13	0.0	0.0	0.0	0.0017348212117187772	0.0
14-15	0.0	0.0	0.0	0.0029113091598958786	0.0
16-17	0.0	0.0	0.0	0.005194493398307373	0.0
18-19	0.0	0.0	0.0	0.00765714190000012	0.0
20-21	0.0	0.0	0.0	0.012393004403255402	0.0
22-23	0.0	0.0	0.0	0.01921264640794301	0.0
24-25	0.0	0.0	0.0	0.02475609809596393	0.0
26-27	0.0	0.0	0.0	0.029521871309766086	0.0
28-29	0.0	0.0	0.0	0.040419340185677714	0.0
30-31	0.0	0.0	0.0	0.05278243387838624	0.0
32-33	0.0	0.0	0.0	0.09935341019987134	0.0
34-35	0.0	0.0	0.0	0.14837706478620022	0.0
36-37	0.0	0.0	0.0	0.19091009518385715	0.0
38-39	0.0	0.0	0.0	0.25197779588372793	0.0
40-41	0.0	0.0	0.0	0.3116197527141976	0.0
42-43	0.0	0.0	0.0	0.38062376194583925	0.0
44-45	0.0	0.0	0.0	0.468361846216674	0.0
46-47	0.0	0.0	0.0	0.5805968024253997	0.0
48-49	0.0	0.0	0.0	0.6506377162893331	0.0
50-51	0.0	0.0	0.0	0.7332909797671989	0.0
52-53	0.0	0.0	0.0	0.816612249113945	0.0
54-55	0.0	0.0	0.0	0.9303128301394676	0.0
56-57	0.0	0.0	0.0	1.1249119129574394	0.0
58-59	0.0	0.0	0.0	1.2481540106474154	0.0
60-61	0.0	0.0	0.0	1.365463817412261	0.0
62-63	0.0	0.0	0.0	1.4815971362289293	0.0
64-65	0.0	0.0	0.0	1.5927154259105718	0.0
66-67	0.0	0.0	0.0	1.748759602833621	0.0
68-69	0.0	0.0	0.0	1.930836068169301	0.0
70-71	0.0	0.0	0.0	2.0866110486974283	0.0
72-73	0.0	0.0	0.0	2.20156787956592	0.0
74-75	0.0	0.0	0.0	2.3654287221785526	0.0
76-77	0.0	0.0	0.0	2.492170373002513	0.0
78-79	0.0	0.0	0.0	2.7938099186309033	0.0
80-81	0.0	0.0	0.0	3.069855866268017	0.0
82-83	0.0	0.0	0.0	3.232011798379477	0.0
84-85	0.0	0.0	0.0	3.413460138793673	0.0
86-87	0.0	0.0	0.0	3.5831336294892484	0.0
88	0.0	0.0	0.0	3.7491480432612563	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAGGT	335	0.0	35.071083	2
GTACTAG	535	0.0	32.518898	1
GTACGCA	9795	0.0	32.193787	3
AGTACGC	9945	0.0	31.80272	2
GAGTACG	10045	0.0	31.69032	1
TACGCAG	10055	0.0	31.361012	4
CGCAGTG	10195	0.0	31.11412	6
GGGCACC	540	0.0	30.465353	94
AGTAAGG	1670	0.0	29.553215	94
GCTTAGG	465	0.0	29.324654	1
ACGCAGT	11140	0.0	28.390923	5
TTAGGTA	415	0.0	28.31039	3
TCTCGGG	715	0.0	27.610558	94
GCATCGT	575	0.0	26.97011	8
GTACCCG	400	0.0	25.861313	1
AGTAAGA	1860	0.0	24.765383	94
GTGATCG	365	0.0	24.462303	8
TAGTACT	790	0.0	24.389696	4
GCTATTG	715	0.0	23.661	8
CGTTAAC	120	1.8337998E-4	23.510286	1
>>END_MODULE