FastQCFastQC Report
Mon 16 Jan 2017
ERR1146535_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146535_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5014926
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT65940413.148828118301248No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1673563.3371579161885943No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1531013.052906463624787No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1252272.4970856997690496No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1153972.3010708433185254No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1100812.195067285140399No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG993611.9813054070987288No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC918511.8315524496273723No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG600401.1972260408229354No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT527511.0518799280388185No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT329720.6574772987677187No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA316150.6304180759596453No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG286380.5710552857609464No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA180580.3600850740369848No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT162720.3244713880125051No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG157420.31390293695260907No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC146440.29200829683229623No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT140910.2809812148773482No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC105470.21031217609193037No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103860.2071017598265657No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA56330.11232468834036634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG4800.039.2314381
CGTTAAC1501.0531949E-931.3851471
GTACGCA115700.031.16963
GAGTACG117400.030.9173131
TACGCAG117000.030.8232734
CGCAGTG117500.030.719876
AGTACGC117700.030.6775972
GGGCACC5150.030.11457494
TAGGCAT7600.029.0772635
ACGCAGT126800.028.4781155
CTAGGCA8750.025.7930364
CTGGGGG6600.025.6464845
CAATGCG3500.024.84126518-19
TATTCTC4950.024.6966155
CGACGAG3550.024.49114424-25
GCATCGT6550.024.395148
TCGACGG1751.649496E-724.1698894
CTTAGGT4100.024.1122572
GTAGGCA47400.024.0052553
CCTGGGG9300.023.7620644
Produced by FastQC (version 0.11.5)