##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146535_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5014926 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.57833714794595 33.0 31.0 34.0 31.0 34.0 2 32.50890481733928 34.0 31.0 34.0 31.0 34.0 3 32.733304539289314 34.0 31.0 34.0 31.0 34.0 4 35.964888215698494 37.0 35.0 37.0 35.0 37.0 5 36.05337606178038 37.0 35.0 37.0 35.0 37.0 6 36.31004106541153 37.0 37.0 37.0 35.0 37.0 7 36.35772272611799 37.0 37.0 37.0 35.0 37.0 8 36.38257413967823 37.0 37.0 37.0 35.0 37.0 9 38.174487519855724 39.0 39.0 39.0 37.0 39.0 10-11 38.14217039294299 39.0 38.5 39.0 37.0 39.0 12-13 38.16711572613434 39.0 39.0 39.0 37.0 39.0 14-15 39.31085024983419 40.0 39.0 41.0 36.5 41.0 16-17 39.5651784293527 40.5 39.5 41.0 37.0 41.0 18-19 39.714596685973035 41.0 40.0 41.0 37.0 41.0 20-21 39.70204714885125 41.0 40.0 41.0 37.0 41.0 22-23 39.61418892322638 41.0 39.5 41.0 37.0 41.0 24-25 39.48408251687064 41.0 39.0 41.0 37.0 41.0 26-27 39.218965045546035 40.5 38.5 41.0 36.5 41.0 28-29 39.26449831961628 40.0 39.0 41.0 36.5 41.0 30-31 39.44437714534571 41.0 39.0 41.0 37.0 41.0 32-33 39.29121606181228 41.0 39.0 41.0 36.0 41.0 34-35 39.00686710033209 40.0 38.5 41.0 35.0 41.0 36-37 38.821098257481765 40.0 38.5 41.0 35.5 41.0 38-39 38.70692738038407 40.0 38.0 41.0 35.0 41.0 40-41 39.01910726100445 40.0 39.0 41.0 35.0 41.0 42-43 38.960817467695435 40.0 38.5 41.0 35.0 41.0 44-45 38.813848997971256 40.0 38.0 41.0 35.0 41.0 46-47 38.60557025168467 40.0 38.0 41.0 34.5 41.0 48-49 38.34575196124529 40.0 38.0 41.0 33.5 41.0 50-51 37.98993115750861 40.0 37.5 41.0 33.5 41.0 52-53 38.285949483601556 40.0 38.0 41.0 34.0 41.0 54-55 38.23406875794379 40.0 38.0 41.0 33.5 41.0 56-57 37.74543223569002 39.5 37.5 41.0 32.0 41.0 58-59 37.494121448651484 39.0 37.0 41.0 32.0 41.0 60-61 36.733999365095315 39.0 35.5 40.0 30.5 41.0 62-63 36.87471290702993 39.0 35.5 40.0 31.0 41.0 64-65 36.58002490963975 38.5 35.5 39.5 31.0 40.5 66-67 35.92125865865219 37.0 35.0 39.0 30.5 40.0 68-69 35.100987731424155 36.5 34.0 39.0 29.5 39.0 70-71 34.23135545768771 35.5 33.5 37.5 29.0 39.0 72-73 33.69705146197571 35.0 33.0 37.0 27.5 37.0 74-75 33.619330773774124 35.0 33.5 37.0 29.0 37.0 76-77 33.44511534168201 35.0 34.0 36.0 29.0 36.5 78-79 33.08326932441277 35.0 33.0 35.5 28.5 36.0 80-81 32.59367825168307 34.0 32.0 35.0 26.5 36.0 82-83 32.23992288221202 34.5 32.0 35.0 26.0 35.5 84-85 32.16583604623478 34.0 32.0 35.0 26.0 35.0 86-87 32.580174862001954 34.5 33.0 35.0 27.0 35.5 88-89 32.384058508540306 35.0 33.0 35.0 26.0 35.0 90-91 32.08138225768436 34.0 32.0 35.0 25.0 35.0 92-93 31.91779729152534 34.0 32.0 35.0 25.0 35.0 94-95 31.73431921029343 34.0 31.5 35.0 25.0 35.0 96-97 31.589406304300404 34.0 31.0 35.0 25.0 35.0 98-99 31.957056195844167 34.0 32.0 35.0 25.0 35.0 100 31.0188894113293 34.0 31.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25752313699687335 1101 2 0.22808230435670396 1101 3 0.2361765455738194 1101 4 0.1640237040350243 1101 5 0.18092719031821503 1101 6 0.15073194001567458 1101 7 0.16047242678774154 1101 8 0.1511358822926212 1101 9 0.18812334162239353 1101 10-11 0.2043283566125922 1101 12-13 0.2015927347783446 1101 14-15 0.3192946315223182 1101 16-17 0.3121952153927978 1101 18-19 0.29467739094041434 1101 20-21 0.3081890214684151 1101 22-23 0.306554059252079 1101 24-25 0.3024919383795108 1101 26-27 0.3032227110897381 1101 28-29 0.3411928726109963 1101 30-31 0.3692915236255345 1101 32-33 0.3610777537653789 1101 34-35 0.3787564792454532 1101 36-37 0.44901630928496417 1101 38-39 0.5026739401492222 1101 40-41 0.47913864298320163 1101 42-43 0.44260812410895056 1101 44-45 0.4861714842787421 1101 46-47 0.5366935292948867 1101 48-49 0.49889110716869567 1101 50-51 0.48463678432246127 1101 52-53 0.6007504844304208 1101 54-55 0.5696891907542962 1101 56-57 0.6210531584324599 1101 58-59 0.640475874552493 1101 60-61 0.6051947270726714 1101 62-63 0.6262576503162052 1101 64-65 0.6010165745707354 1101 66-67 0.587947371659908 1101 68-69 0.48658376768982947 1101 70-71 0.48309085845005484 1101 72-73 0.42858981426434895 1101 74-75 0.40115483553940123 1101 76-77 0.47142641626439286 1101 78-79 0.45893175054536783 1101 80-81 0.5200218110531978 1101 82-83 0.491607607429728 1101 84-85 0.43807344509070845 1101 86-87 0.4843913902353947 1101 88-89 0.4938977613780935 1101 90-91 0.5515910159419093 1101 92-93 0.5353112245606582 1101 94-95 0.5480720037915461 1101 96-97 0.39343742098098744 1101 98-99 0.5017360248050906 1101 100 0.6056392335431227 1102 1 0.23159826631982128 1102 2 0.23744673867201982 1102 3 0.232862023419095 1102 4 0.19191915994607456 1102 5 0.16289485728918152 1102 6 0.13795581941050727 1102 7 0.13878694830034988 1102 8 0.14636895794022564 1102 9 0.1706075221428236 1102 10-11 0.18930225572662351 1102 12-13 0.1907423287373149 1102 14-15 0.317999019825038 1102 16-17 0.3080221275158195 1102 18-19 0.2812039642012536 1102 20-21 0.2641031645365999 1102 22-23 0.27859775696616396 1102 24-25 0.29138991498455624 1102 26-27 0.2824544743665669 1102 28-29 0.32738012908904324 1102 30-31 0.31205122752432146 1102 32-33 0.2962488308353386 1102 34-35 0.3549428288068768 1102 36-37 0.4086797088857068 1102 38-39 0.441592861109001 1102 40-41 0.3965198350425396 1102 42-43 0.387016703124182 1102 44-45 0.4179852232771779 1102 46-47 0.39434120073699575 1102 48-49 0.45002016700436087 1102 50-51 0.45547732138456354 1102 52-53 0.4188891843198448 1102 54-55 0.4409102013029127 1102 56-57 0.5033440226484913 1102 58-59 0.5416790069516111 1102 60-61 0.601362240199137 1102 62-63 0.5094689154792889 1102 64-65 0.5208680421228848 1102 66-67 0.47911704214866546 1102 68-69 0.4409087519022563 1102 70-71 0.4714766364870542 1102 72-73 0.4789372357637234 1102 74-75 0.4297773272927188 1102 76-77 0.4113924393815438 1102 78-79 0.4209176246831632 1102 80-81 0.4966662035974281 1102 82-83 0.4597644186831147 1102 84-85 0.49974236329585153 1102 86-87 0.41745463740942057 1102 88-89 0.43703326676862275 1102 90-91 0.5699788212496415 1102 92-93 0.5221968017070182 1102 94-95 0.49148902319296184 1102 96-97 0.4434000257595869 1102 98-99 0.49174460827914146 1102 100 0.5543093447121983 1103 1 0.20432541363150136 1103 2 0.1946334342663576 1103 3 0.24636479094225194 1103 4 0.17889003012166427 1103 5 0.11633461373665455 1103 6 0.09224420618855333 1103 7 0.08172301177158658 1103 8 0.11120041819163617 1103 9 0.18477312249189026 1103 10-11 0.1785493505598552 1103 12-13 0.13515757795121885 1103 14-15 0.2769426625027762 1103 16-17 0.23945947347553442 1103 18-19 0.24318407807633946 1103 20-21 0.2327990555454349 1103 22-23 0.24194677066941495 1103 24-25 0.2759917757643464 1103 26-27 0.3011459359245805 1103 28-29 0.31097554111291714 1103 30-31 0.276544909371637 1103 32-33 0.268705741742032 1103 34-35 0.33571706260594425 1103 36-37 0.3908160244650958 1103 38-39 0.3848845576046358 1103 40-41 0.36108321375694175 1103 42-43 0.35682202049461864 1103 44-45 0.34406805298137755 1103 46-47 0.4115221568576146 1103 48-49 0.4431031206419789 1103 50-51 0.4471596564496565 1103 52-53 0.41856849664105056 1103 54-55 0.43035785847687436 1103 56-57 0.49961277185253294 1103 58-59 0.5337930795049886 1103 60-61 0.5452206783713578 1103 62-63 0.4984981292181345 1103 64-65 0.4899080963297848 1103 66-67 0.4948016761956424 1103 68-69 0.4997053846987427 1103 70-71 0.5335965317445073 1103 72-73 0.5415768304478874 1103 74-75 0.4572843194972265 1103 76-77 0.41236564160313094 1103 78-79 0.4679801234931418 1103 80-81 0.56333157050247 1103 82-83 0.5913721930362854 1103 84-85 0.4880946292535242 1103 86-87 0.4789171497186757 1103 88-89 0.5196148875234883 1103 90-91 0.5395314375956914 1103 92-93 0.5122739425597942 1103 94-95 0.5408558511554844 1103 96-97 0.5006962775776813 1103 98-99 0.4456228974599128 1103 100 0.4656014343287076 1104 1 0.17663771658762073 1104 2 0.18581269319592764 1104 3 0.21125155330269507 1104 4 0.15013560706614015 1104 5 0.13127174514869466 1104 6 0.09933326074884974 1104 7 0.08752724751952456 1104 8 0.10176753365470859 1104 9 0.12508572217291203 1104 10-11 0.11817176812596841 1104 12-13 0.10551757943483864 1104 14-15 0.22632020784604379 1104 16-17 0.1712909337329407 1104 18-19 0.16653068984889785 1104 20-21 0.1817276561166281 1104 22-23 0.19568884255102148 1104 24-25 0.19531316592050274 1104 26-27 0.22239173541273516 1104 28-29 0.2731417812375341 1104 30-31 0.22179901715695394 1104 32-33 0.20570273740244005 1104 34-35 0.3064540530736579 1104 36-37 0.3246294586848961 1104 38-39 0.33815394672224386 1104 40-41 0.2658288834279432 1104 42-43 0.24699745909111215 1104 44-45 0.2716051621390534 1104 46-47 0.3517332766232002 1104 48-49 0.3662593833918777 1104 50-51 0.3660602906991315 1104 52-53 0.35409685241420163 1104 54-55 0.34377768711824075 1104 56-57 0.4547964816555776 1104 58-59 0.4715838142700193 1104 60-61 0.552958967010504 1104 62-63 0.4290784489392152 1104 64-65 0.38933749434914944 1104 66-67 0.3665806161459457 1104 68-69 0.44689547408711405 1104 70-71 0.43109261868490023 1104 72-73 0.4665713039466439 1104 74-75 0.36748334772318003 1104 76-77 0.28981098858581333 1104 78-79 0.31952925000921795 1104 80-81 0.39770982056737836 1104 82-83 0.40322294041212814 1104 84-85 0.3550939245318929 1104 86-87 0.3987987564840054 1104 88-89 0.341456510608694 1104 90-91 0.34126327246828225 1104 92-93 0.38898445033027684 1104 94-95 0.4259914173436812 1104 96-97 0.35242240049597484 1104 98-99 0.29153232218451564 1104 100 0.30836067916384025 1105 1 0.13747817435585574 1105 2 0.16872177697300117 1105 3 0.14178545933664566 1105 4 0.10469871690894195 1105 5 0.07226926409978773 1105 6 0.0793323389362115 1105 7 0.06834340473761102 1105 8 0.06565464102627061 1105 9 0.09381152213956767 1105 10-11 0.08677737941523844 1105 12-13 0.09802890105918749 1105 14-15 0.16857306889750134 1105 16-17 0.15830495705284164 1105 18-19 0.15559334368864342 1105 20-21 0.12726101298560621 1105 22-23 0.1395764892810405 1105 24-25 0.16608893989988616 1105 26-27 0.18574606449984543 1105 28-29 0.22136776262476587 1105 30-31 0.18894274147613999 1105 32-33 0.235322656400605 1105 34-35 0.24273478424759531 1105 36-37 0.2003588968695098 1105 38-39 0.24164209554595573 1105 40-41 0.15904337157676451 1105 42-43 0.17042366014432986 1105 44-45 0.20505098603361915 1105 46-47 0.2145464624665152 1105 48-49 0.28996258914241935 1105 50-51 0.26693880756143784 1105 52-53 0.2517178893553549 1105 54-55 0.2297573974337226 1105 56-57 0.3229834625230623 1105 58-59 0.3111002254769062 1105 60-61 0.3007277293299424 1105 62-63 0.2771196657318953 1105 64-65 0.2526573143014872 1105 66-67 0.24429357938131346 1105 68-69 0.2547058160403708 1105 70-71 0.26437833156968793 1105 72-73 0.26766087667667904 1105 74-75 0.21918926007252537 1105 76-77 0.18544736292020048 1105 78-79 0.2469000932716483 1105 80-81 0.280723255496234 1105 82-83 0.26898133724265705 1105 84-85 0.2789021973099821 1105 86-87 0.25682167431519787 1105 88-89 0.2507165990634803 1105 90-91 0.2918334626166583 1105 92-93 0.31972365580623574 1105 94-95 0.30026308729553364 1105 96-97 0.26759569146515005 1105 98-99 0.2637796131340835 1105 100 0.30388276834430883 1106 1 0.09306890213927232 1106 2 0.11869572750877211 1106 3 0.09902445668232218 1106 4 0.11377646458933555 1106 5 0.12451110061549997 1106 6 0.09817665746260218 1106 7 0.08442681240001093 1106 8 0.07449547468509365 1106 9 0.12937228512615917 1106 10-11 0.11534577619257647 1106 12-13 0.12291349845094857 1106 14-15 0.2068418446106648 1106 16-17 0.15941146581058518 1106 18-19 0.15720501581103008 1106 20-21 0.13863802944519676 1106 22-23 0.1624555469677773 1106 24-25 0.18024285140260332 1106 26-27 0.1607407549036992 1106 28-29 0.198386034282386 1106 30-31 0.23136040079725717 1106 32-33 0.22510338511458627 1106 34-35 0.23122700612446323 1106 36-37 0.2159621065559918 1106 38-39 0.26888617934230297 1106 40-41 0.234400032421604 1106 42-43 0.2279101669649819 1106 44-45 0.22713827294551692 1106 46-47 0.21723595886675184 1106 48-49 0.20547033687978455 1106 50-51 0.22502310685617033 1106 52-53 0.20992218868806845 1106 54-55 0.21180083388663462 1106 56-57 0.2829003242178203 1106 58-59 0.2968662743840653 1106 60-61 0.34515369789206574 1106 62-63 0.31900620717966177 1106 64-65 0.25685331958607804 1106 66-67 0.20524347800046172 1106 68-69 0.22478272051575487 1106 70-71 0.27187301247798956 1106 72-73 0.20150437700300472 1106 74-75 0.20402502184052196 1106 76-77 0.20449832839626936 1106 78-79 0.21335614595803776 1106 80-81 0.20981941505189639 1106 82-83 0.22889562722541967 1106 84-85 0.23694103664967514 1106 86-87 0.19076195014198305 1106 88-89 0.2085811554597754 1106 90-91 0.19650567774534977 1106 92-93 0.23360070075248274 1106 94-95 0.22004225082603313 1106 96-97 0.2627456009092377 1106 98-99 0.267023689333989 1106 100 0.3182229509247847 1107 1 0.10443003966496889 1107 2 0.11525488795925298 1107 3 0.1453015820706085 1107 4 0.119973100330796 1107 5 0.05550492048683253 1107 6 0.06996025509572235 1107 7 0.05922258163826655 1107 8 0.06361982184525772 1107 9 0.07098847749954018 1107 10-11 0.08451381402677072 1107 12-13 0.07807160374601096 1107 14-15 0.16172290381031473 1107 16-17 0.11650572500575862 1107 18-19 0.08718015056471984 1107 20-21 0.0982228295003722 1107 22-23 0.10965812938079011 1107 24-25 0.11670463783297436 1107 26-27 0.17069588073177755 1107 28-29 0.15758059691285808 1107 30-31 0.16520758542823444 1107 32-33 0.12804123069448536 1107 34-35 0.16207539429762363 1107 36-37 0.20168190761043547 1107 38-39 0.19297127505087985 1107 40-41 0.19442650710713139 1107 42-43 0.19480137152707044 1107 44-45 0.1545635445604887 1107 46-47 0.24648003680840702 1107 48-49 0.24634644513785986 1107 50-51 0.26799244409146183 1107 52-53 0.2755014571065644 1107 54-55 0.2649296456193895 1107 56-57 0.29344122991352606 1107 58-59 0.26373426588057214 1107 60-61 0.272991351459396 1107 62-63 0.26270789084173174 1107 64-65 0.27246664857254643 1107 66-67 0.30868027184427405 1107 68-69 0.23344045705468375 1107 70-71 0.23069229721490103 1107 72-73 0.2619261812492155 1107 74-75 0.19302740829105147 1107 76-77 0.1778678942544616 1107 78-79 0.2222242370864933 1107 80-81 0.3012919369723903 1107 82-83 0.2613558938263765 1107 84-85 0.23130527278800628 1107 86-87 0.179924211975937 1107 88-89 0.21641387017105274 1107 90-91 0.18405842667342398 1107 92-93 0.26128589237891475 1107 94-95 0.2558243808038725 1107 96-97 0.3024646299634455 1107 98-99 0.23295648905666866 1107 100 0.2443040018119511 1108 1 0.031515738413844474 1108 2 0.031597193817560765 1108 3 0.047653266246470594 1108 4 0.048270984880751655 1108 5 0.0610161849884463 1108 6 0.027731934598826058 1108 7 0.03030774206296627 1108 8 0.04096908082973272 1108 9 0.026732082167384874 1108 10-11 0.027718666868892683 1108 12-13 0.027088376506256395 1108 14-15 0.03836165003419012 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2214 100 -0.30146378968028387 2215 1 -0.326128584230311 2215 2 -0.3338768968113044 2215 3 -0.3112540665301964 2215 4 -0.2697513871397277 2215 5 -0.22598721484279594 2215 6 -0.21126283417367375 2215 7 -0.21299138434564924 2215 8 -0.21694344584381753 2215 9 -0.2608443152192521 2215 10-11 -0.2694197215792542 2215 12-13 -0.27343152098825385 2215 14-15 -0.39420657029990025 2215 16-17 -0.4041662891192104 2215 18-19 -0.39796439119191973 2215 20-21 -0.4053210711187418 2215 22-23 -0.3487662923579933 2215 24-25 -0.3489861915507717 2215 26-27 -0.4332736946338329 2215 28-29 -0.4002866947841426 2215 30-31 -0.4170916281972765 2215 32-33 -0.43932407560372866 2215 34-35 -0.5320508108430886 2215 36-37 -0.47283902218051566 2215 38-39 -0.48353024912608333 2215 40-41 -0.47444312162127744 2215 42-43 -0.5169038388095757 2215 44-45 -0.5648854641338019 2215 46-47 -0.6083606037585696 2215 48-49 -0.6099617404279201 2215 50-51 -0.6501803841945417 2215 52-53 -0.6589667729743027 2215 54-55 -0.7240629026431336 2215 56-57 -0.7585139760406392 2215 58-59 -0.7703294315091398 2215 60-61 -0.8465843789091991 2215 62-63 -0.7926151408184907 2215 64-65 -0.6590484852411151 2215 66-67 -0.7318303233550765 2215 68-69 -0.7348083242080392 2215 70-71 -0.7476546668055448 2215 72-73 -0.7192656236578756 2215 74-75 -0.6561572765314523 2215 76-77 -0.647979453845295 2215 78-79 -0.6477162869664568 2215 80-81 -0.6780565866659316 2215 82-83 -0.6790226662839665 2215 84-85 -0.6889822101679641 2215 86-87 -0.6265595367600909 2215 88-89 -0.6049287937943397 2215 90-91 -0.7993216231529026 2215 92-93 -0.7473985055954131 2215 94-95 -0.7413644907281025 2215 96-97 -0.7693484835222719 2215 98-99 -0.7416054563760888 2215 100 -0.6480732460591732 2216 1 -0.4025545240197985 2216 2 -0.3866104642157495 2216 3 -0.36260662247404696 2216 4 -0.2694875958915972 2216 5 -0.23109782318161365 2216 6 -0.24435314771996275 2216 7 -0.25185347096959276 2216 8 -0.2551528625270123 2216 9 -0.30269651934887776 2216 10-11 -0.3292846051309013 2216 12-13 -0.3144823653578186 2216 14-15 -0.4725006046335736 2216 16-17 -0.4627970564263464 2216 18-19 -0.4495279955606577 2216 20-21 -0.45956190469167524 2216 22-23 -0.460320597905266 2216 24-25 -0.5192981001737351 2216 26-27 -0.555956329656027 2216 28-29 -0.5993023204612982 2216 30-31 -0.6409599837970958 2216 32-33 -0.7649047469885559 2216 34-35 -0.9425559584336227 2216 36-37 -0.9457090693147165 2216 38-39 -0.9586415911439516 2216 40-41 -0.9641213505994557 2216 42-43 -1.0549818278025 2216 44-45 -1.1581293146764509 2216 46-47 -1.3006914403960153 2216 48-49 -1.3173542962985465 2216 50-51 -1.2761057695493392 2216 52-53 -1.3854865391456244 2216 54-55 -1.4922846764807929 2216 56-57 -1.7465589001118502 2216 58-59 -1.7555270093028241 2216 60-61 -1.8311947923510274 2216 62-63 -1.825093808333925 2216 64-65 -1.621172859616209 2216 66-67 -1.8081245783478153 2216 68-69 -1.8535311337837328 2216 70-71 -1.9018488736041803 2216 72-73 -1.7341788741833142 2216 74-75 -1.7260058566526908 2216 76-77 -1.6878429959181744 2216 78-79 -1.789803807287381 2216 80-81 -1.975502049702257 2216 82-83 -1.9234374188151762 2216 84-85 -1.9688191445997845 2216 86-87 -1.8989308137785592 2216 88-89 -1.9454072738894332 2216 90-91 -2.091136670254091 2216 92-93 -2.1830100289350547 2216 94-95 -2.2076266133757514 2216 96-97 -2.034297410142827 2216 98-99 -2.123316370005199 2216 100 -2.5211043374932522 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 5.0 8 46.0 9 86.0 10 125.0 11 147.0 12 130.0 13 174.0 14 271.0 15 361.0 16 635.0 17 1433.0 18 2676.0 19 4033.0 20 5972.0 21 9037.0 22 13286.0 23 17354.0 24 20983.0 25 24414.0 26 29435.0 27 35921.0 28 45069.0 29 55974.0 30 70032.0 31 88000.0 32 113750.0 33 152460.0 34 221527.0 35 369996.0 36 794749.0 37 1577154.0 38 1263049.0 39 96312.0 40 330.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.8898351643421 15.626772937031658 12.728573647791833 15.754818250834408 2 12.001920288083136 32.4375581999994 25.93303145020675 29.627490061710716 3 41.319898438673064 18.858298498813834 28.627284965346274 11.194518097166824 4 9.776255920825152 12.81931179044317 48.90734180324895 28.497090485482733 5 9.185754199416792 47.0094303602783 32.7550520072181 11.049763433086813 6 24.576401651479184 38.57464998596115 12.876037758123388 23.972910604436276 7 24.73279261404707 33.788441502116186 22.31453112459309 19.16423475924366 8 21.94080231692352 15.257014759539821 19.079144936535457 43.723037987001206 9 14.5363859805708 30.87650744996038 31.43190946386846 23.15519710560036 10-11 29.384152428171422 18.03201084123674 25.79223502001824 26.791601710573598 12-13 41.3609792048776 19.406796830102778 20.234994494435213 18.99722947058441 14-15 25.185478901123815 24.800168935777386 36.453726562329415 13.560625600769383 16-17 18.02254908646708 27.032223406686363 32.46720888802746 22.4780186188191 18-19 15.742495502426157 41.910119910044536 22.32304125723889 20.024343330290417 20-21 24.3662718054065 24.419293524969262 18.37031094775875 32.844123721865486 22-23 18.67701874872505 27.065349844393737 23.724007712216054 30.533623694665156 24-25 31.92044109923058 25.85463873245587 19.93634601986151 22.288574148452042 26-27 26.876887116579585 16.31394959766106 37.40095068202402 19.40821260373533 28-29 17.16926318325063 40.5643535176206 23.595671639291044 18.670711659837718 30-31 22.808111472122597 22.998373653667993 22.48406854583182 31.709446328377588 32-33 25.393808165414434 25.794777566970396 26.816962393564786 21.994451874050384 34-35 32.73698259294074 21.194401760207956 25.634404296668585 20.434211350182714 36-37 26.21222310570277 17.91982372485923 36.48544458873704 19.382508580700964 38-39 20.978100922023938 29.309068800391792 31.210206641559445 18.50262363602483 40-41 21.027319423066302 32.2909207580595 24.39756507513574 22.284194743738457 42-43 18.292961608775137 31.71577237095246 16.74109492998459 33.250171090287814 44-45 25.05983917327971 20.29551732880842 21.368689169406252 33.27595432850562 46-47 32.88549217378854 29.189832999013642 23.453479379143456 14.471195448054358 48-49 27.612102501806117 26.122101809867683 25.767817364942893 20.49797832338331 50-51 11.905680835312856 29.249340089882374 38.67467615175814 20.17030292304663 52-53 23.35289913680748 34.620434978200834 19.418102795046412 22.608563089945267 54-55 17.64724474169899 23.371625887998988 26.49171516672511 32.489414203576914 56-57 28.661849532899808 18.20531191492832 26.030588586719077 27.102249965452792 58-59 39.45780333588312 20.22921533922363 20.661046772715345 19.65193455217791 60-61 24.560788491913698 25.224827756185192 36.306120695001376 13.908263056899733 62-63 18.359485842031283 26.717872953664486 32.0430363324818 22.87960487182243 64-65 16.027971735126993 41.07075092153975 22.118180431351394 20.78309691198186 66-67 24.3409890737494 24.35366178556216 18.46306427966352 32.84228486102492 68-69 18.918175166892702 26.99563826624224 23.74295303713107 30.343233529733993 70-71 31.325799243666385 25.894869647659487 19.968740842822733 22.810590265851395 72-73 26.431846099850837 16.925546595196504 36.527864190736 20.114743114216658 74-75 17.447449088250757 39.946559712975066 23.170489252119143 19.43550194665503 76-77 22.798636663478742 23.127904926979497 22.226604843785022 31.84685356575674 78-79 25.292486679409393 25.71092087527804 26.399889608668115 22.596702836644457 80-81 32.07830625582636 21.438286714158238 25.290099054322845 21.19330797569256 82-83 25.72931174427331 18.520208661500146 35.57845878390996 20.17202081031658 84-85 20.74043808848112 29.345066713885736 30.428612762349495 19.485882435283653 86-87 21.00412269375913 32.21910596868348 24.023509823177815 22.753261514379577 88-89 18.420428966093866 31.521210165230002 16.92038464832479 33.13797622035134 90-91 24.906075365035367 20.5707064412728 21.306141168611695 33.21707702508014 92-93 32.11586771170701 28.97941066328795 23.324800804637995 15.579920820367041 94-95 26.85125631477477 26.194446956390475 25.67848714801446 21.275809580820294 96-97 12.355252929189279 29.0460659176139 37.68787345499517 20.91080769820165 98-99 23.119459387134462 33.98297394106085 19.796123592469723 23.101443079334967 100 21.663083006190963 14.810591097593148 20.778657003711544 42.74766889250434 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 227.0 1 143.0 2 163.0 3 288.0 4 185.0 5 103.0 6 205.5 7 474.5 8 840.5 9 987.0 10 726.0 11 422.5 12 411.5 13 499.5 14 586.5 15 714.5 16 834.0 17 983.0 18 1223.0 19 1462.0 20 1697.5 21 1953.0 22 2249.5 23 2624.5 24 3096.5 25 3662.5 26 4263.5 27 5005.5 28 6142.0 29 7698.0 30 9419.0 31 10834.5 32 12744.0 33 14362.0 34 15797.0 35 17810.5 36 19492.5 37 21936.0 38 22904.5 39 22568.0 40 22677.0 41 22524.0 42 23063.5 43 25439.5 44 31692.5 45 42460.0 46 73676.5 47 507096.0 48 1485812.0 49 1500949.5 50 608480.5 51 181503.5 52 67085.0 53 37182.0 54 29776.5 55 24960.5 56 21837.5 57 18468.5 58 15035.5 59 13929.5 60 11713.0 61 8443.5 62 6293.5 63 5104.5 64 3695.5 65 2158.0 66 1466.0 67 1133.0 68 887.0 69 677.5 70 476.5 71 328.5 72 269.0 73 284.5 74 231.5 75 149.5 76 109.5 77 78.0 78 46.0 79 38.5 80 36.5 81 18.5 82 5.5 83 3.5 84 1.5 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005882439740885508 2 0.10490683212474121 3 4.985118424479244E-4 4 0.0 5 0.03924285223750062 6 0.005383927898437584 7 1.994047369791698E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.985118424479244E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.985118424479244E-4 24-25 0.0 26-27 0.0 28-29 3.988094739583396E-5 30-31 7.976189479166792E-5 32-33 0.0015852676589843998 34-35 0.0012761903166666868 36-37 7.677082373698037E-4 38-39 6.879463425781357E-4 40-41 0.0013758926851562714 42-43 5.982142109375093E-4 44-45 5.08482079296883E-4 46-47 3.090773423177131E-4 48-49 5.782737372395923E-4 50-51 7.677082373698037E-4 52-53 0.001076785579687517 54-55 0.00138586292200523 56-57 9.77083211197932E-4 58-59 0.0024327377911458714 60-61 0.0017348212117187772 62-63 9.37202263802098E-4 64-65 0.004406844687239652 66-67 0.0042672613713542334 68-69 0.003529463844531305 70-71 0.001505505764192732 72-73 0.004905356529687577 74-75 0.0033898805286458862 76-77 0.0033898805286458862 78-79 7.876487110677207E-4 80-81 1.994047369791698E-5 82-83 1.894345001302113E-4 84-85 5.9821421093750935E-5 86-87 1.994047369791698E-5 88-89 1.4955355273437733E-4 90-91 1.994047369791698E-5 92-93 0.0 94-95 9.97023684895849E-6 96-97 2.8913686861979616E-4 98-99 4.985118424479244E-4 100 6.979165794270942E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5014926.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.54783018785052 #Duplication Level Percentage of deduplicated Percentage of total 1 75.3084087952644 33.548262067286664 2 15.06072105537093 13.41844888162498 3 4.931399449737913 6.590494358261523 4 2.18682223039577 3.8967274148274917 5 1.050093195059621 2.33896866674667 6 0.5483435165751646 1.4656508316599537 7 0.30710183318625345 0.9576504220611172 8 0.1955825213959237 0.6970221560685801 9 0.10925970367275549 0.4380554453029902 >10 0.2964427205766421 1.787571253890494 >50 0.002518286120125852 0.07258568814888322 >100 0.0016853857988031176 0.16748750449853267 >500 2.0785985758375164E-4 0.06250301267737511 >1k 5.404356130942254E-4 0.6093997287369052 >5k 4.157197023801729E-5 0.10431984051464734 >10k+ 8.314394047603458E-4 33.844852727693144 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 659404 13.148828118301248 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 167356 3.3371579161885943 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 153101 3.052906463624787 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 125227 2.4970856997690496 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 115397 2.3010708433185254 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 110081 2.195067285140399 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 99361 1.9813054070987288 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 91851 1.8315524496273723 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 60040 1.1972260408229354 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 52751 1.0518799280388185 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 32972 0.6574772987677187 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 31615 0.6304180759596453 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 28638 0.5710552857609464 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 18058 0.3600850740369848 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16272 0.3244713880125051 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 15742 0.31390293695260907 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 14644 0.29200829683229623 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 14091 0.2809812148773482 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 10547 0.21031217609193037 No Hit GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10386 0.2071017598265657 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5633 0.11232468834036634 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.9940473697916977E-5 0.0 5 0.0 0.0 0.0 1.9940473697916977E-5 0.0 6 0.0 0.0 0.0 1.9940473697916977E-5 0.0 7 0.0 0.0 0.0 1.9940473697916977E-5 0.0 8 0.0 0.0 0.0 3.9880947395833954E-5 0.0 9 0.0 0.0 0.0 2.1934521067708677E-4 0.0 10-11 0.0 0.0 0.0 9.870534480468905E-4 0.0 12-13 0.0 0.0 0.0 0.001754761685416694 0.0 14-15 0.0 0.0 0.0 0.002931249633593796 0.0 16-17 0.0 0.0 0.0 0.0052343743457032065 0.0 18-19 0.0 0.0 0.0 0.007706993084244912 0.0 20-21 0.0 0.0 0.0 0.012502677008593945 0.0 22-23 0.0 0.0 0.0 0.019372170197526344 0.0 24-25 0.0 0.0 0.0 0.024905651648698304 0.0 26-27 0.0 0.0 0.0 0.029661454625651507 0.0 28-29 0.0 0.0 0.0 0.04046919136992251 0.0 30-31 0.0 0.0 0.0 0.05274255293099041 0.0 32-33 0.0 0.0 0.0 0.09904433285755362 0.0 34-35 0.0 0.0 0.0 0.1479782553122419 0.0 36-37 0.0 0.0 0.0 0.19020220836758112 0.0 38-39 0.0 0.0 0.0 0.2508411888829466 0.0 40-41 0.0 0.0 0.0 0.310323621923833 0.0 42-43 0.0 0.0 0.0 0.3789487621552142 0.0 44-45 0.0 0.0 0.0 0.46639770955742915 0.0 46-47 0.0 0.0 0.0 0.5782737372395923 0.0 48-49 0.0 0.0 0.0 0.6481850380244893 0.0 50-51 0.0 0.0 0.0 0.7306588372390739 0.0 52-53 0.0 0.0 0.0 0.8138405232699346 0.0 54-55 0.0 0.0 0.0 0.9271422948214989 0.0 56-57 0.0 0.0 0.0 1.1204253063754082 0.0 58-59 0.0 0.0 0.0 1.2426504399067904 0.0 60-61 0.0 0.0 0.0 1.3593420919870005 0.0 62-63 0.0 0.0 0.0 1.4752261548824448 0.0 64-65 0.0 0.0 0.0 1.5858259922479414 0.0 66-67 0.0 0.0 0.0 1.7412520144863555 0.0 68-69 0.0 0.0 0.0 1.9230592834271132 0.0 70-71 0.0 0.0 0.0 2.078445424718131 0.0 72-73 0.0 0.0 0.0 2.1931230889548523 0.0 74-75 0.0 0.0 0.0 2.357233187488709 0.0 76-77 0.0 0.0 0.0 2.4843636775497786 0.0 78-79 0.0 0.0 0.0 2.7862125981519967 0.0 80-81 0.0 0.0 0.0 3.0622386053154127 0.0 82-83 0.0 0.0 0.0 3.2245939421638523 0.0 84-85 0.0 0.0 0.0 3.406341389683517 0.0 86-87 0.0 0.0 0.0 3.575855356589509 0.0 88 0.0 0.0 0.0 3.74165042515084 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 480 0.0 39.231438 1 CGTTAAC 150 1.0531949E-9 31.385147 1 GTACGCA 11570 0.0 31.1696 3 GAGTACG 11740 0.0 30.917313 1 TACGCAG 11700 0.0 30.823273 4 CGCAGTG 11750 0.0 30.71987 6 AGTACGC 11770 0.0 30.677597 2 GGGCACC 515 0.0 30.114574 94 TAGGCAT 760 0.0 29.077263 5 ACGCAGT 12680 0.0 28.478115 5 CTAGGCA 875 0.0 25.793036 4 CTGGGGG 660 0.0 25.646484 5 CAATGCG 350 0.0 24.841265 18-19 TATTCTC 495 0.0 24.696615 5 CGACGAG 355 0.0 24.491144 24-25 GCATCGT 655 0.0 24.39514 8 TCGACGG 175 1.649496E-7 24.16988 94 CTTAGGT 410 0.0 24.112257 2 GTAGGCA 4740 0.0 24.005255 3 CCTGGGG 930 0.0 23.762064 4 >>END_MODULE