FastQCFastQC Report
Mon 16 Jan 2017
ERR1146534_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146534_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4185687
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2408135.75324910821091No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC579991.3856506709651246No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT565351.3506743337473635No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC445231.0636963537885178No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA417190.9967061560025868No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG383080.9152141571980896No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG209510.5005390990774035No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG95610.22842128424796215No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC58540.13985756698960053No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG57250.13677563563639614No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT48160.11505877051963034No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG42140.10067642420467655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15250.027.4397471
GAGTACG27000.025.4243011
GCATCGT14750.025.1678338
GTACCCG4400.024.5773531
GTACGCA28300.023.7443563
TACGCAG28850.023.6174494
AGTACGC28500.023.577732
GCTTATG13900.022.3248791
CGCAGTG31300.021.6178936
GGGCACC19200.021.29922594
CGACGAG8900.021.11860524-25
CATCGTC17650.021.032619
TAGGCAT23650.020.8623755
CATGGGG58150.020.2831234
CTAGGCA25350.020.0196534
GTCCTAC14850.019.9468361
TAGTACT24650.019.6350084
CTAGTAC23050.019.5709133
GTATAAG14700.019.5106811
CTTTGCG16150.019.203582
Produced by FastQC (version 0.11.5)