##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146534_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4185687
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.845783499817355	33.0	31.0	34.0	30.0	34.0
2	31.961255344702078	33.0	31.0	34.0	30.0	34.0
3	32.06224474023022	34.0	31.0	34.0	30.0	34.0
4	35.46874097370396	37.0	35.0	37.0	33.0	37.0
5	35.56638922117206	37.0	35.0	37.0	35.0	37.0
6	35.656817387444406	37.0	36.0	37.0	35.0	37.0
7	35.67285298685736	37.0	36.0	37.0	35.0	37.0
8	35.6448305857557	37.0	36.0	37.0	35.0	37.0
9	37.31552741521284	39.0	38.0	39.0	35.0	39.0
10-11	37.2992110972464	39.0	38.0	39.0	35.0	39.0
12-13	37.29674017670217	39.0	38.0	39.0	35.0	39.0
14-15	38.6153718612978	40.0	39.0	41.0	35.0	41.0
16-17	38.72697516560603	40.5	39.0	41.0	35.0	41.0
18-19	38.7948332018137	41.0	39.0	41.0	35.0	41.0
20-21	38.82164863259006	41.0	39.0	41.0	35.0	41.0
22-23	38.79366385972004	41.0	39.0	41.0	35.0	41.0
24-25	38.75961592445876	40.5	39.0	41.0	35.0	41.0
26-27	38.64904566442737	40.5	38.5	41.0	34.5	41.0
28-29	38.64131670619423	40.0	38.5	41.0	34.5	41.0
30-31	38.69429606179344	40.5	39.0	41.0	34.5	41.0
32-33	38.60738547817837	40.0	38.0	41.0	34.0	41.0
34-35	38.384111616563786	40.0	38.0	41.0	34.0	41.0
36-37	38.22456265841187	40.0	38.0	41.0	33.5	41.0
38-39	38.12209489147182	40.0	38.0	41.0	33.0	41.0
40-41	38.21658881803632	40.0	38.0	41.0	33.0	41.0
42-43	38.03178988299889	40.0	38.0	41.0	33.0	41.0
44-45	37.80345329213579	40.0	38.0	41.0	32.5	41.0
46-47	37.56574452413666	40.0	37.0	41.0	32.0	41.0
48-49	37.34005074913628	40.0	37.0	41.0	31.0	41.0
50-51	37.07824641450735	39.0	36.0	41.0	30.5	41.0
52-53	37.10301248038852	39.0	36.0	41.0	31.0	41.0
54-55	36.85845883841769	39.0	35.5	41.0	30.5	41.0
56-57	36.40186342170353	39.0	35.0	40.5	29.0	41.0
58-59	36.12871937151536	38.5	35.0	40.0	28.0	41.0
60-61	35.63069873595422	38.0	34.0	40.0	28.0	41.0
62-63	35.63738628808126	38.0	34.0	40.0	28.0	41.0
64-65	35.39944673359475	37.0	34.0	39.5	28.0	41.0
66-67	34.83471936625935	36.5	33.5	39.0	27.0	41.0
68-69	34.39143036734471	36.0	33.0	39.0	26.5	41.0
70-71	33.872415925987774	35.0	32.5	38.0	26.0	40.0
72-73	33.48571011162564	35.0	32.5	37.0	26.0	39.5
74-75	33.33653734739363	35.0	32.0	37.0	26.0	39.0
76-77	33.050028585510574	35.0	32.0	36.5	26.0	39.0
78-79	32.63540644582359	35.0	32.0	36.0	26.0	38.5
80-81	32.1857337875479	35.0	31.0	35.5	25.0	37.0
82-83	31.8605525210079	34.5	30.5	35.0	24.0	37.0
84-85	31.77973102145478	34.0	31.0	35.0	24.0	36.0
86-87	31.929587186046163	34.5	31.0	35.0	25.0	36.0
88-89	31.6826322656233	34.0	31.0	35.0	24.5	36.0
90-91	31.359796133824627	34.0	31.0	35.0	23.0	35.5
92-93	31.251613534409046	34.0	31.0	35.0	23.0	35.0
94-95	31.16511889207196	34.0	30.5	35.0	21.5	35.0
96-97	31.036702696594368	34.0	30.5	35.0	21.5	35.0
98-99	31.203431838071026	34.0	31.0	35.0	23.0	35.0
100	29.89713086525581	34.0	29.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24395792834337726
1101	2	0.25457857755900903
1101	3	0.3075605245109756
1101	4	0.23172592996527897
1101	5	0.2568897349007315
1101	6	0.21611042340096986
1101	7	0.24268818143463022
1101	8	0.27961904012567373
1101	9	0.32711613263521855
1101	10-11	0.3990486812060823
1101	12-13	0.3551531180219172
1101	14-15	0.5206825665149708
1101	16-17	0.4999619552466399
1101	18-19	0.45753967812743923
1101	20-21	0.4205966139771746
1101	22-23	0.4403961687112101
1101	24-25	0.42738146716362024
1101	26-27	0.4917262937923539
1101	28-29	0.5178411426519034
1101	30-31	0.4832325843614953
1101	32-33	0.49772566022325293
1101	34-35	0.5073808971370042
1101	36-37	0.5040360970453222
1101	38-39	0.4672842645442472
1101	40-41	0.4330163036234609
1101	42-43	0.40271128353154495
1101	44-45	0.4255727388957311
1101	46-47	0.550189220667896
1101	48-49	0.5272620818800036
1101	50-51	0.5315163785018058
1101	52-53	0.49190297408030403
1101	54-55	0.5116755228310623
1101	56-57	0.5545425827550403
1101	58-59	0.4809594284833807
1101	60-61	0.5732041411940116
1101	62-63	0.5401568864923192
1101	64-65	0.4741904844387932
1101	66-67	0.5034759857311144
1101	68-69	0.5189747833803722
1101	70-71	0.5217711889551424
1101	72-73	0.4797656042716625
1101	74-75	0.43944694159286257
1101	76-77	0.4321949874214184
1101	78-79	0.533940230342715
1101	80-81	0.4902687211781398
1101	82-83	0.4569969532833227
1101	84-85	0.4531275517021207
1101	86-87	0.4406087609787903
1101	88-89	0.36794773232246314
1101	90-91	0.4035296435578317
1101	92-93	0.43460099881259495
1101	94-95	0.3841565851909863
1101	96-97	0.31221791597172555
1101	98-99	0.342552665100758
1101	100	0.292004409479933
1102	1	0.15733149128524104
1102	2	0.15559243636392495
1102	3	0.22806556761828034
1102	4	0.15848150798416327
1102	5	0.18198873700943352
1102	6	0.1319489085626131
1102	7	0.1113626169689681
1102	8	0.14920461724896228
1102	9	0.24796564815258648
1102	10-11	0.32248952839530887
1102	12-13	0.28478224457641943
1102	14-15	0.3808854220166751
1102	16-17	0.36763620371272765
1102	18-19	0.2926689837309553
1102	20-21	0.2895376714407618
1102	22-23	0.3603539342931228
1102	24-25	0.36028664769087015
1102	26-27	0.3228182855255852
1102	28-29	0.3642180153391905
1102	30-31	0.33202330097906696
1102	32-33	0.3478640524043044
1102	34-35	0.3846693251491544
1102	36-37	0.45575446366984806
1102	38-39	0.4122533067363747
1102	40-41	0.3560521970240842
1102	42-43	0.3359116500888035
1102	44-45	0.3204918347173944
1102	46-47	0.3219403930734259
1102	48-49	0.3473903733692367
1102	50-51	0.3545203652696358
1102	52-53	0.35086513633376626
1102	54-55	0.41848432801140945
1102	56-57	0.4484244823692194
1102	58-59	0.4837738262662654
1102	60-61	0.4782529187427542
1102	62-63	0.41468841749831853
1102	64-65	0.42115434762234827
1102	66-67	0.450395193112449
1102	68-69	0.4701960459434247
1102	70-71	0.5099027696094538
1102	72-73	0.44795003951219314
1102	74-75	0.4074174299445943
1102	76-77	0.45302850704236164
1102	78-79	0.4620577482337751
1102	80-81	0.48124532175067714
1102	82-83	0.43290703746829706
1102	84-85	0.382928772528242
1102	86-87	0.3861370385869236
1102	88-89	0.4452407341390696
1102	90-91	0.4386601343054046
1102	92-93	0.45442947014566215
1102	94-95	0.37915894980175935
1102	96-97	0.3641480720970698
1102	98-99	0.35528098807082387
1102	100	0.15719285377184633
1103	1	0.036928667589815944
1103	2	0.09016351959246549
1103	3	0.177858825623737
1103	4	0.10851088757669913
1103	5	0.12399158982161396
1103	6	0.17751421401109724
1103	7	0.10732444228258942
1103	8	0.13088724740236302
1103	9	0.25396752942420875
1103	10-11	0.202890713436382
1103	12-13	0.16030684248918448
1103	14-15	0.2853832134661971
1103	16-17	0.2541546187020387
1103	18-19	0.17338440602312488
1103	20-21	0.21409406202698733
1103	22-23	0.19110051077285561
1103	24-25	0.19504303881951301
1103	26-27	0.21773294575308455
1103	28-29	0.1960589979639309
1103	30-31	0.2474006490235965
1103	32-33	0.24300348350730872
1103	34-35	0.2929025219357655
1103	36-37	0.3548332613475651
1103	38-39	0.28457472714633525
1103	40-41	0.3064345368704835
1103	42-43	0.30940122613436216
1103	44-45	0.27694539897308346
1103	46-47	0.31790571735270845
1103	48-49	0.31855199882997454
1103	50-51	0.3782998883783577
1103	52-53	0.3166219145585032
1103	54-55	0.3423766350046904
1103	56-57	0.3524217040900339
1103	58-59	0.4116222412967616
1103	60-61	0.36685080753048993
1103	62-63	0.3677296531602181
1103	64-65	0.32664032063732407
1103	66-67	0.439353713092153
1103	68-69	0.4351428387295613
1103	70-71	0.4051765174660815
1103	72-73	0.4441455589008285
1103	74-75	0.33898673546420355
1103	76-77	0.3746751477390049
1103	78-79	0.38020502147309543
1103	80-81	0.3689990725163028
1103	82-83	0.36191945290340755
1103	84-85	0.39016039792770485
1103	86-87	0.3017541076329735
1103	88-89	0.2919950952649444
1103	90-91	0.3739836290830212
1103	92-93	0.34533496864129276
1103	94-95	0.24900889506889712
1103	96-97	0.33895809751428985
1103	98-99	0.18484158260569572
1103	100	0.15734114964665835
1104	1	0.16535298096382434
1104	2	0.17163913290456634
1104	3	0.1363131421640702
1104	4	0.15036680031644778
1104	5	0.11700435863323122
1104	6	0.08530950359139666
1104	7	0.048846031841605964
1104	8	0.10200218715948495
1104	9	0.13203002810411135
1104	10-11	0.16392119456224208
1104	12-13	0.15642964549769545
1104	14-15	0.2298332011325286
1104	16-17	0.21013154513651244
1104	18-19	0.18417490864903385
1104	20-21	0.16687426974298347
1104	22-23	0.2229047463487035
1104	24-25	0.22178884549162348
1104	26-27	0.22850939917869084
1104	28-29	0.2017260821881166
1104	30-31	0.16027075824131742
1104	32-33	0.2130479602171249
1104	34-35	0.26532384617516414
1104	36-37	0.30734314142378594
1104	38-39	0.3303215037867062
1104	40-41	0.3257226075033657
1104	42-43	0.309472141145406
1104	44-45	0.2995085102267936
1104	46-47	0.3073673835362527
1104	48-49	0.372438662306422
1104	50-51	0.33532558283654623
1104	52-53	0.2746526047020552
1104	54-55	0.2542913508156559
1104	56-57	0.3033596506837668
1104	58-59	0.37042549409759573
1104	60-61	0.3754427536687217
1104	62-63	0.3057204015228905
1104	64-65	0.32674014641768423
1104	66-67	0.3318498058375283
1104	68-69	0.37228700330820175
1104	70-71	0.32239580990439265
1104	72-73	0.35347455284716034
1104	74-75	0.3611504002372854
1104	76-77	0.34314100087095767
1104	78-79	0.3497908921445614
1104	80-81	0.33868797065663614
1104	82-83	0.311295262225741
1104	84-85	0.35057085057095705
1104	86-87	0.26746784667088974
1104	88-89	0.30102180203012807
1104	90-91	0.3963727500704337
1104	92-93	0.40864166870491303
1104	94-95	0.39697488147711013
1104	96-97	0.40729665724570907
1104	98-99	0.27848690949283395
1104	100	0.26887851540631047
1105	1	0.1912784543163042
1105	2	0.22230895350824653
1105	3	0.21936996704722844
1105	4	0.2222842696811611
1105	5	0.18912868660318338
1105	6	0.18900357677380697
1105	7	0.19544256767404988
1105	8	0.19856931561511715
1105	9	0.24564941932143824
1105	10-11	0.287834888719658
1105	12-13	0.2876116976467813
1105	14-15	0.34628238244076215
1105	16-17	0.33358965820136177
1105	18-19	0.2834404836059008
1105	20-21	0.308428464780242
1105	22-23	0.2591983015059611
1105	24-25	0.3001487783173715
1105	26-27	0.31539405936761966
1105	28-29	0.3528864303373993
1105	30-31	0.288243059901248
1105	32-33	0.31172137184513105
1105	34-35	0.28751892002966173
1105	36-37	0.3516843296774681
1105	38-39	0.374036213311598
1105	40-41	0.380521719596544
1105	42-43	0.4161337793151887
1105	44-45	0.4065227832893896
1105	46-47	0.4027748688708357
1105	48-49	0.36223406552836934
1105	50-51	0.38370655267821263
1105	52-53	0.3900019872558431
1105	54-55	0.47090709391193997
1105	56-57	0.4369908078712186
1105	58-59	0.5231209015799081
1105	60-61	0.4666093054625904
1105	62-63	0.4489739863499054
1105	64-65	0.412332793302312
1105	66-67	0.3706888903443186
1105	68-69	0.3725051091518665
1105	70-71	0.3560707815017139
1105	72-73	0.3357802765803797
1105	74-75	0.39114708705749734
1105	76-77	0.3941280086951977
1105	78-79	0.35461988263985234
1105	80-81	0.3851336304469726
1105	82-83	0.36173017406444785
1105	84-85	0.40989144309299164
1105	86-87	0.3440311350626786
1105	88-89	0.37344224185844865
1105	90-91	0.37638005156626164
1105	92-93	0.36262868791226666
1105	94-95	0.2992333148753836
1105	96-97	0.27418542441280636
1105	98-99	0.28055993405278556
1105	100	0.15188353354666972
1106	1	0.17143018713350244
1106	2	0.13979836463725803
1106	3	0.15143937813595443
1106	4	0.11268953406414539
1106	5	0.13353300668416068
1106	6	0.10111524264397076
1106	7	0.11483212044836222
1106	8	0.10714451005012648
1106	9	0.1882465893501788
1106	10-11	0.17473141802136638
1106	12-13	0.1358244147650325
1106	14-15	0.1573615740468881
1106	16-17	0.15769272234916087
1106	18-19	0.09543723350369504
1106	20-21	0.09656448397471706
1106	22-23	0.15298150277033074
1106	24-25	0.18987627151779662
1106	26-27	0.21220466639383062
1106	28-29	0.2112321524795533
1106	30-31	0.18741171736102302
1106	32-33	0.20398366280466007
1106	34-35	0.18781081350480378
1106	36-37	0.20174473680916094
1106	38-39	0.2411279357650713
1106	40-41	0.260446353823383
1106	42-43	0.1369108259286662
1106	44-45	0.21386815527446856
1106	46-47	0.20377022705966397
1106	48-49	0.19525114496511264
1106	50-51	0.21635352154645915
1106	52-53	0.17673107702293578
1106	54-55	0.2988656982669511
1106	56-57	0.3257472841228264
1106	58-59	0.35052896520549837
1106	60-61	0.2630086433578427
1106	62-63	0.2707942104677059
1106	64-65	0.3137572737419987
1106	66-67	0.2899631243361469
1106	68-69	0.2374743387746321
1106	70-71	0.18684966090123112
1106	72-73	0.263810724997235
1106	74-75	0.23969433797913808
1106	76-77	0.2478646048288553
1106	78-79	0.2312282962498884
1106	80-81	0.30917601412763673
1106	82-83	0.261138446000416
1106	84-85	0.2400242294485473
1106	86-87	0.19184577508134026
1106	88-89	0.239601832925608
1106	90-91	0.2648276156152285
1106	92-93	0.20495335689586014
1106	94-95	0.17757188426072545
1106	96-97	0.08284520897269232
1106	98-99	0.12154517307599733
1106	100	0.02151786539833367
1107	1	0.17719475306619614
1107	2	0.1242724964905193
1107	3	0.11531041117935814
1107	4	0.14249134472269986
1107	5	0.1242013125681396
1107	6	0.07603257962830412
1107	7	0.10896854464133554
1107	8	0.11088672257893961
1107	9	0.15202698447980367
1107	10-11	0.1505338946680368
1107	12-13	0.12165752309631728
1107	14-15	0.22069872635815102
1107	16-17	0.17660441593216802
1107	18-19	0.12783572482793915
1107	20-21	0.11431975676199357
1107	22-23	0.12433677243494401
1107	24-25	0.11292294640926315
1107	26-27	0.13938290381886986
1107	28-29	0.18618529238202086
1107	30-31	0.16481803014230678
1107	32-33	0.18154666046186207
1107	34-35	0.155323057160615
1107	36-37	0.1312158146465876
1107	38-39	0.14366707602663098
1107	40-41	0.16176417040529856
1107	42-43	0.22464193309218672
1107	44-45	0.1579255707721785
1107	46-47	0.19165458686445191
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2216	84-85	-1.3381309497520597
2216	86-87	-1.3097897227625168
2216	88-89	-1.3422854143408784
2216	90-91	-1.4033105543595035
2216	92-93	-1.4149671795105654
2216	94-95	-1.3898754921889456
2216	96-97	-1.224959634913528
2216	98-99	-1.289262950298447
2216	100	-1.4217700509674707
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	103.0
1	70.0
2	50.0
3	45.0
4	43.0
5	59.0
6	163.0
7	680.0
8	2214.0
9	3031.0
10	2943.0
11	3674.0
12	4439.0
13	5313.0
14	6351.0
15	7078.0
16	7649.0
17	8413.0
18	9350.0
19	10505.0
20	12130.0
21	14344.0
22	16679.0
23	20571.0
24	25391.0
25	31222.0
26	36727.0
27	39433.0
28	46796.0
29	58375.0
30	72040.0
31	90192.0
32	121558.0
33	171490.0
34	261027.0
35	403446.0
36	627228.0
37	949759.0
38	871209.0
39	243419.0
40	478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.6463033253983	17.72889156417981	12.927519962286684	20.6972851481352
2	13.492116208746049	25.299811530653987	33.75302487565993	27.45504738494004
3	28.42415126618648	22.787366449194245	29.66167726551029	19.126805019108982
4	11.13387597875325	15.143601448785379	44.04825079402929	29.674271778432075
5	11.078512049148522	41.7415736243403	34.991011165079165	12.18890316143202
6	27.565045915818253	37.87048472949295	16.1785967988313	18.385872555857496
7	24.809207997268718	33.11345933262758	23.218006397681183	18.859326272422525
8	23.581785015010315	25.809356449896125	20.705395231820674	29.903463303272883
9	20.518862909320365	22.270745592458326	26.23891290235565	30.97147859586566
10-11	26.460418412321136	22.317306881337068	27.34647618515155	23.875798521190244
12-13	32.070155645653685	21.98601030023989	24.71203421674179	21.231799837364633
14-15	23.947402123259128	24.791709674706304	30.778273485256847	20.48261471677772
16-17	20.33120560114716	27.59795000842834	29.459627067257422	22.611217323167082
18-19	19.219087019448157	34.26370387714789	25.539252023273995	20.977957080129954
20-21	23.57977246828226	26.1222090295192	22.85408221375548	27.443936288443062
22-23	20.847124685289593	27.024049633098674	25.478880655887792	26.649945025723948
24-25	27.1443196628388	26.323982477747766	23.427853309075182	23.10384455033825
26-27	24.581149540189422	22.145546973359167	31.600283695500643	21.67301979095077
28-29	20.08381216699214	33.27167898260406	25.49620642368808	21.148302426715723
30-31	22.565264876444672	25.446617690382816	24.888281364426035	27.099836068746473
32-33	23.79487313045548	26.65311747654378	26.72220544369695	22.82980394930379
34-35	27.244991010585995	24.693595922106958	25.937825439904255	22.123587627402795
36-37	24.19006758173519	22.889186384726635	31.360099595393713	21.560646438144463
38-39	22.46879014436679	27.940574867332423	28.560052627353222	21.030582360947562
40-41	21.88387189889013	29.53383977157948	26.20048493242273	22.381803397107657
42-43	20.7594214964708	29.027335321707216	22.071201022592284	28.142042159229703
44-45	23.71133688022328	24.18549025202706	24.238841766696275	27.864331101053384
46-47	27.323798959636658	27.86116348483123	25.631622284992357	19.183415270539754
48-49	25.004633339997056	26.20845333912954	26.67142885991875	22.115484460954658
50-51	17.520680177261735	27.815994518584976	32.825194338579664	21.838130965573626
52-53	22.624968588547674	30.568806883406786	23.43568577578311	23.370538752262433
54-55	20.00002867978695	25.21261580808347	26.993391579590654	27.79396393253893
56-57	25.592321232885716	22.615627849393107	26.12978473974099	25.662266177980186
58-59	30.15522010227711	23.870772919438554	24.2892357609946	21.684771217289743
60-61	23.899979541617515	25.975704714796766	31.191600399990055	18.932715343595667
62-63	20.678124321119277	26.889818718980603	29.255532078779677	23.176524881120443
64-65	19.26696037516425	33.35236129108736	25.069320399579944	22.311357934168445
66-67	23.351840733903558	25.845045950232453	22.81939546262367	27.98371785324032
68-69	20.727166782345662	26.719888565752512	25.78321134457236	26.769733307329457
70-71	26.72794368075564	26.05553769653717	23.62525857402178	23.59126004868541
72-73	24.48753593026249	22.149377160984066	31.521132025237165	21.841954883516284
74-75	20.547626806703718	32.587892717449456	25.439399355000692	21.42508112084613
76-77	22.378819227931537	25.364066328892587	25.027873650921272	27.2292407922546
78-79	23.81729988225609	26.24349603216286	26.899028352824473	23.040175732756577
80-81	26.89513635784574	24.44409867297851	26.005077582273493	22.655687386902258
82-83	23.991685566817505	23.013219609376037	31.153586973576676	21.84150785022978
84-85	22.48231923460784	27.83605642931709	28.019663458413156	21.661960877661915
86-87	21.911093563886027	29.384307491114324	26.020609598247148	22.6839893467525
88-89	21.042963383576314	28.688770137312336	22.419193543202667	27.84907293590868
90-91	23.676050878267183	23.986822526887657	24.447202397937797	27.889924196907362
92-93	27.217328344880553	27.585116999165788	25.529692498906304	19.667862157047356
94-95	24.4432165020795	26.31325667483538	26.52347143183723	22.720055391247893
96-97	18.13328448351474	27.539443719105893	32.168274719525805	22.158997077853563
98-99	23.019118092344325	30.091320992619902	23.648772178117518	23.24078873691825
100	22.3519189076546	21.2777072523506	24.287124945990694	32.08324889400411
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1358.0
1	935.0
2	529.5
3	517.5
4	450.0
5	363.5
6	299.5
7	282.5
8	321.5
9	373.5
10	331.0
11	260.0
12	253.0
13	271.5
14	306.0
15	404.0
16	480.0
17	549.5
18	733.5
19	971.0
20	1368.5
21	2074.5
22	3016.0
23	4216.0
24	5861.0
25	7744.5
26	10436.0
27	13922.5
28	17682.5
29	22353.0
30	27254.0
31	32167.5
32	38352.0
33	43474.5
34	47950.5
35	54352.5
36	61657.5
37	68989.5
38	72067.5
39	72251.5
40	72769.5
41	73635.0
42	76252.5
43	80504.0
44	86613.0
45	97275.0
46	124704.0
47	299959.5
48	664966.0
49	661011.5
50	329704.5
51	174389.5
52	122141.5
53	104443.0
54	95891.0
55	84463.5
56	74962.0
57	64647.5
58	54801.0
59	50105.5
60	42228.5
61	32060.0
62	25162.0
63	20402.5
64	15028.0
65	9889.5
66	7063.0
67	5395.5
68	4179.0
69	3254.0
70	2502.0
71	1955.0
72	1667.5
73	1457.0
74	1081.0
75	757.5
76	546.5
77	365.5
78	243.5
79	162.5
80	121.5
81	75.5
82	52.0
83	40.0
84	31.5
85	35.5
86	29.5
87	23.5
88	20.5
89	12.5
90	12.0
91	15.5
92	13.5
93	9.5
94	8.5
95	7.0
96	6.5
97	6.5
98	5.5
99	6.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060898007901689734
2	0.009341357822503211
3	0.01151543342825204
4	0.009269685000335667
5	0.01182601565764473
6	0.012614416701487713
7	0.0025802215980315775
8	0.005542698247623388
9	0.007262845979644441
10-11	0.007023936572419294
12-13	0.005984680650989909
14-15	0.01010586792562368
16-17	0.009592212700089614
18-19	0.007872064968068564
20-21	0.006988100161335524
22-23	0.0022218574871938584
24-25	0.0036314229898222203
26-27	0.005100715844256868
28-29	0.006128026295324996
30-31	0.009460812526115785
32-33	0.010583686740073971
34-35	0.011622942661503356
36-37	0.018025714775137272
38-39	0.018897734111509055
40-41	0.018491588119226306
42-43	0.024559887062745017
44-45	0.028095746289677176
46-47	0.0306640224173475
48-49	0.032025806038530835
50-51	0.03263502502695496
52-53	0.031189623113242818
54-55	0.037162358293871475
56-57	0.040184562295269566
58-59	0.037365431290012845
60-61	0.03781935916374062
62-63	0.0336862264187456
64-65	0.035669174498714304
66-67	0.0381060504524108
68-69	0.040495144524662255
70-71	0.040184562295269566
72-73	0.0367920487126725
74-75	0.039455888603232875
76-77	0.03889445149625378
78-79	0.03838079627071972
80-81	0.04314703894486138
82-83	0.03912141543311767
84-85	0.03533470132859911
86-87	0.03399680864813829
88-89	0.03157187816480305
90-91	0.03404459052958332
92-93	0.03560944714690802
94-95	0.0390019607295051
96-97	0.03425960899608595
98-99	0.03167938739805437
100	0.029218620503635367
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4185687.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.520237867333265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.66565437410055	39.96123582621954
2	14.095837844958453	15.088251888030694
3	5.158714480753622	8.282868782987714
4	2.417144475346746	5.174645891210732
5	1.3118622554564823	3.510558998060362
6	0.7467866824257323	2.3980920527749117
7	0.4650557767205345	1.7422927054162305
8	0.3029904732873687	1.2972897761500686
9	0.2018276364501755	0.9721676799913549
>10	0.6283345174117255	4.783973508085096
>50	0.0036034894516086854	0.12230768852260822
>100	0.0010965657825138273	0.12039001059500924
>500	2.297742852255463E-4	0.0773824869148134
>1k	2.8721785643362176E-4	0.42057704437255755
>5k	1.7233071386017304E-4	0.649926806873967
>10k+	4.021049990070704E-4	15.398038853794352
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	240813	5.75324910821091	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	57999	1.3856506709651246	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	56535	1.3506743337473635	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	44523	1.0636963537885178	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	41719	0.9967061560025868	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	38308	0.9152141571980896	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	20951	0.5005390990774035	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	9561	0.22842128424796215	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC	5854	0.13985756698960053	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	5725	0.13677563563639614	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT	4816	0.11505877051963034	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	4214	0.10067642420467655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.778188144502921E-5	0.0
10-11	0.0	0.0	0.0	2.9863675903143256E-4	0.0
12-13	0.0	0.0	0.0	4.7781881445029217E-4	0.0
14-15	0.0	0.0	0.0	6.808918105916664E-4	0.0
16-17	0.0	0.0	0.0	0.0013617836211833327	0.0
18-19	0.0	0.0	0.0	0.0021621301353875718	0.0
20-21	0.0	0.0	0.0	0.003523913756570905	0.0
22-23	2.3890940722514606E-5	0.0	0.0	0.005614371069790933	0.0
24-25	2.3890940722514606E-5	0.0	0.0	0.007501755386869587	0.0
26-27	2.3890940722514606E-5	0.0	0.0	0.00979528569623099	0.0
28-29	2.3890940722514606E-5	0.0	0.0	0.015899421050833473	0.0
30-31	2.3890940722514606E-5	0.0	0.0	0.02724761789402791	0.0
32-33	2.3890940722514606E-5	0.0	0.0	0.06153111783083637	0.0
34-35	2.3890940722514606E-5	0.0	0.0	0.10519181200123182	0.0
36-37	2.3890940722514606E-5	0.0	0.0	0.15697542601728223	0.0
38-39	2.3890940722514606E-5	0.0	0.0	0.23169434312694667	0.0
40-41	2.3890940722514606E-5	0.0	0.0	0.3084320447276636	0.0
42-43	2.3890940722514606E-5	0.0	0.0	0.39040186234661123	0.0
44-45	2.3890940722514606E-5	0.0	0.0	0.4907796497922563	0.0
46-47	2.3890940722514606E-5	0.0	0.0	0.6166490709888246	0.0
48-49	2.3890940722514606E-5	0.0	0.0	0.7171104767269985	0.0
50-51	2.3890940722514606E-5	0.0	0.0	0.8235685085865236	0.0
52-53	2.3890940722514606E-5	0.0	0.0	0.9292978667540119	0.0
54-55	2.3890940722514606E-5	0.0	0.0	1.059109293169795	0.0
56-57	2.3890940722514606E-5	0.0	0.0	1.2478955067591055	0.0
58-59	2.3890940722514606E-5	0.0	0.0	1.396102957531225	0.0
60-61	2.3890940722514606E-5	0.0	0.0	1.541562950120255	0.0
62-63	2.3890940722514606E-5	0.0	0.0	1.6867601423613374	0.0
64-65	2.3890940722514606E-5	0.0	0.0	1.8298788227595613	0.0
66-67	2.3890940722514606E-5	0.0	0.0	2.0057400374179912	0.0
68-69	2.3890940722514606E-5	0.0	0.0	2.214045149577596	0.0
70-71	2.3890940722514606E-5	0.0	0.0	2.416401417497295	0.0
72-73	2.3890940722514606E-5	0.0	0.0	2.58509534993897	0.0
74-75	2.3890940722514606E-5	0.0	0.0	2.784883341730999	0.0
76-77	2.3890940722514606E-5	0.0	0.0	2.9653674534192356	0.0
78-79	2.3890940722514606E-5	0.0	0.0	3.261591227437694	0.0
80-81	2.3890940722514606E-5	0.0	0.0	3.5490231352702675	0.0
82-83	2.3890940722514606E-5	0.0	0.0	3.766729332604182	0.0
84-85	4.778188144502921E-5	0.0	0.0	4.001051679210605	0.0
86-87	4.778188144502921E-5	0.0	0.0	4.234705079476798	0.0
88	4.778188144502921E-5	0.0	0.0	4.437551111681309	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1525	0.0	27.439747	1
GAGTACG	2700	0.0	25.424301	1
GCATCGT	1475	0.0	25.167833	8
GTACCCG	440	0.0	24.577353	1
GTACGCA	2830	0.0	23.744356	3
TACGCAG	2885	0.0	23.617449	4
AGTACGC	2850	0.0	23.57773	2
GCTTATG	1390	0.0	22.324879	1
CGCAGTG	3130	0.0	21.617893	6
GGGCACC	1920	0.0	21.299225	94
CGACGAG	890	0.0	21.118605	24-25
CATCGTC	1765	0.0	21.03261	9
TAGGCAT	2365	0.0	20.862375	5
CATGGGG	5815	0.0	20.283123	4
CTAGGCA	2535	0.0	20.019653	4
GTCCTAC	1485	0.0	19.946836	1
TAGTACT	2465	0.0	19.635008	4
CTAGTAC	2305	0.0	19.570913	3
GTATAAG	1470	0.0	19.510681	1
CTTTGCG	1615	0.0	19.20358	2
>>END_MODULE