FastQCFastQC Report
Mon 16 Jan 2017
ERR1146534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4185687
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2623016.266617642456304No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC674121.6105360959861548No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT622881.48811891572399No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC520871.2444074294136183No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA472211.1281541118578624No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG438541.0477133144451556No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA435041.0393514851922754No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC368710.8808828753798361No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG250230.597823009699483No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT215720.5153753732660851No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG116580.2785205869430753No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT80400.19208316340901746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68940.1647041453410157No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA65340.15610340668091044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65220.15581671539224026No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT51270.12248885308433238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC3650.027.0772041
GAGTACG35750.025.9338841
GTACGCA36550.025.2127533
TACGCAG36700.025.1094044
AGTACGC38200.024.1447852
GCTTATG14750.023.6111451
CGCAGTG39100.023.558596
GTATAAG12650.023.0663341
CTCGTCG11900.021.7212949
GTACCCG3301.8189894E-1221.3921621
CGACGAG8700.021.3377124-25
GAGTACT84700.021.25129312-13
CAATGCG9450.020.8875518-19
GCATCGT18200.020.6580168
TGCGACG9250.020.57705722-23
TAGGCAT22300.020.2401075
TAGGACT17500.020.1497484
TACTAGG23150.019.9207522
CTAGGCA24400.019.6542634
CTTTGCG13650.019.6504652
Produced by FastQC (version 0.11.5)