##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146534_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4185687
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55923627351974	34.0	31.0	34.0	31.0	34.0
2	32.55801903008992	34.0	31.0	34.0	31.0	34.0
3	32.71669740236191	34.0	31.0	34.0	31.0	34.0
4	36.087366064399944	37.0	35.0	37.0	35.0	37.0
5	36.13717556998409	37.0	35.0	37.0	35.0	37.0
6	36.295907457963295	37.0	37.0	37.0	35.0	37.0
7	36.31050338928831	37.0	37.0	37.0	35.0	37.0
8	36.33040836546068	37.0	37.0	37.0	35.0	37.0
9	38.13718130380987	39.0	39.0	39.0	37.0	39.0
10-11	38.10379502815189	39.0	38.0	39.0	37.0	39.0
12-13	38.10288036348633	39.0	38.5	39.0	37.0	39.0
14-15	39.42610400634352	41.0	39.0	41.0	36.5	41.0
16-17	39.54144803469538	41.0	39.0	41.0	37.0	41.0
18-19	39.62331213490163	41.0	40.0	41.0	37.0	41.0
20-21	39.63256055696472	41.0	40.0	41.0	37.0	41.0
22-23	39.57596650203419	41.0	39.5	41.0	37.0	41.0
24-25	39.506135910305765	41.0	39.0	41.0	37.0	41.0
26-27	39.352216493971	41.0	39.0	41.0	36.5	41.0
28-29	39.338567241172115	41.0	39.0	41.0	36.0	41.0
30-31	39.37790654198463	41.0	39.0	41.0	36.0	41.0
32-33	39.24983521223636	41.0	39.0	41.0	35.5	41.0
34-35	39.09369119095622	40.0	39.0	41.0	35.0	41.0
36-37	38.97024204150955	40.0	38.5	41.0	35.0	41.0
38-39	38.874791760587925	40.0	38.0	41.0	35.0	41.0
40-41	38.97939489980976	40.0	39.0	41.0	35.0	41.0
42-43	38.91599121004509	40.0	39.0	41.0	35.0	41.0
44-45	38.78506300160524	40.0	38.0	41.0	35.0	41.0
46-47	38.58182814434046	40.0	38.0	41.0	35.0	41.0
48-49	38.349887127250554	40.0	38.0	41.0	34.0	41.0
50-51	38.1250469755622	40.0	38.0	41.0	34.0	41.0
52-53	38.20452628206552	40.0	38.0	41.0	34.0	41.0
54-55	38.11772249095549	40.0	37.5	41.0	34.0	41.0
56-57	37.81844437962036	40.0	37.0	41.0	33.5	41.0
58-59	37.582364973778496	39.5	36.5	41.0	33.0	41.0
60-61	37.140333713438196	39.0	36.0	41.0	32.0	41.0
62-63	37.049719436737625	39.0	35.0	41.0	32.0	41.0
64-65	36.7563437017627	38.5	35.0	40.5	32.0	41.0
66-67	36.35695084223928	37.5	35.0	39.0	31.5	41.0
68-69	35.82767034419918	37.0	35.0	39.0	31.0	41.0
70-71	35.263874245733135	36.0	34.5	38.5	30.5	40.5
72-73	34.834178594816095	36.0	34.0	37.0	30.0	40.0
74-75	34.60569172993585	35.0	34.0	37.0	30.5	39.0
76-77	34.34814894185829	35.0	34.0	36.5	30.5	39.0
78-79	34.04421138035405	35.0	34.0	36.0	30.0	38.5
80-81	33.72223376951024	35.0	34.0	36.0	29.0	37.0
82-83	33.406693453189405	35.0	33.5	35.0	29.0	37.0
84-85	33.25608125977887	35.0	34.0	35.0	29.0	36.5
86-87	33.32407690780509	35.0	34.0	35.0	29.0	36.0
88-89	33.18731930982895	35.0	34.0	35.0	29.0	36.0
90-91	33.035798305033325	35.0	34.0	35.0	29.0	36.0
92-93	32.92432329507677	35.0	34.0	35.0	29.0	35.5
94-95	32.76475749381165	35.0	33.0	35.0	28.5	35.0
96-97	32.706027708235226	35.0	33.5	35.0	28.5	35.0
98-99	32.80935339885663	35.0	34.0	35.0	29.0	35.0
100	32.10374497663108	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3075305729816762
1101	2	0.31195524007732445
1101	3	0.300561462328929
1101	4	0.2175510192305552
1101	5	0.19806802025154724
1101	6	0.18758647773305626
1101	7	0.17139956185074823
1101	8	0.18197071834120493
1101	9	0.20807731969584609
1101	10-11	0.2343497304133706
1101	12-13	0.2332925520509903
1101	14-15	0.3734771405286139
1101	16-17	0.3540778977204724
1101	18-19	0.3472941556564564
1101	20-21	0.347064533799049
1101	22-23	0.3662045905546023
1101	24-25	0.3241174964922351
1101	26-27	0.37280695140594844
1101	28-29	0.3833384898936316
1101	30-31	0.3453103617210971
1101	32-33	0.37358096675601615
1101	34-35	0.3918146197481889
1101	36-37	0.4096741826791259
1101	38-39	0.3777384598627549
1101	40-41	0.32129135438250955
1101	42-43	0.33678545213390265
1101	44-45	0.35677339137964026
1101	46-47	0.43756972070644196
1101	48-49	0.4685312446389034
1101	50-51	0.5027637722276808
1101	52-53	0.502225476457653
1101	54-55	0.503711929253086
1101	56-57	0.5204712872222785
1101	58-59	0.5457369507433256
1101	60-61	0.4723869911863403
1101	62-63	0.4860278032903622
1101	64-65	0.5298150611231023
1101	66-67	0.48863331385246056
1101	68-69	0.495160068939704
1101	70-71	0.478147469523563
1101	72-73	0.46177108914952925
1101	74-75	0.4388680831392193
1101	76-77	0.4103522065323233
1101	78-79	0.42474308122516646
1101	80-81	0.44874949499590855
1101	82-83	0.42011335700308905
1101	84-85	0.46786414682015476
1101	86-87	0.428412408332818
1101	88-89	0.4120820243259402
1101	90-91	0.38072330690845035
1101	92-93	0.3447314353684803
1101	94-95	0.36826264237762985
1101	96-97	0.4086217518276598
1101	98-99	0.4661555614999031
1101	100	0.5136907368895081
1102	1	0.17023660175981803
1102	2	0.21217810393323333
1102	3	0.20986064903624424
1102	4	0.13466002140029332
1102	5	0.08068876265701164
1102	6	0.12419677696135523
1102	7	0.12890487335415202
1102	8	0.12557808678631233
1102	9	0.19504240279715646
1102	10-11	0.178829882940704
1102	12-13	0.2036457122056845
1102	14-15	0.2856688771081295
1102	16-17	0.2795433186942873
1102	18-19	0.27988506010871816
1102	20-21	0.269924110408823
1102	22-23	0.27442792193918564
1102	24-25	0.2506291264457019
1102	26-27	0.24862965073263155
1102	28-29	0.3184053341473998
1102	30-31	0.36004949093401706
1102	32-33	0.29863816947256083
1102	34-35	0.32723407984197905
1102	36-37	0.37040652790037143
1102	38-39	0.4082935139202206
1102	40-41	0.375231092574964
1102	42-43	0.3466142704941788
1102	44-45	0.40106483278022864
1102	46-47	0.4272607415866858
1102	48-49	0.44646130751399227
1102	50-51	0.4316751059944721
1102	52-53	0.39125683693064417
1102	54-55	0.4766631984004306
1102	56-57	0.46609735695671617
1102	58-59	0.47110748922595036
1102	60-61	0.5183806578332764
1102	62-63	0.43867764346622806
1102	64-65	0.45837299841606693
1102	66-67	0.41689540867334784
1102	68-69	0.3743384468876769
1102	70-71	0.40117136955019816
1102	72-73	0.4106490486919725
1102	74-75	0.3768904602202596
1102	76-77	0.3589248318052469
1102	78-79	0.34480240016900154
1102	80-81	0.3640973518083612
1102	82-83	0.4436301456686209
1102	84-85	0.46178205139462847
1102	86-87	0.38506145325574437
1102	88-89	0.40893461525665487
1102	90-91	0.43717168281400376
1102	92-93	0.4242015006876656
1102	94-95	0.39471340773230423
1102	96-97	0.33461796830848556
1102	98-99	0.3465574232689619
1102	100	0.478132902356343
1103	1	0.16437012543688212
1103	2	0.1627981223227195
1103	3	0.15501193476884367
1103	4	0.14162547428748695
1103	5	0.09681092497493182
1103	6	0.07601638810472622
1103	7	0.10160212620404252
1103	8	0.1034634853816172
1103	9	0.14060536109259658
1103	10-11	0.1440120272066352
1103	12-13	0.14250560655567313
1103	14-15	0.21900726971143314
1103	16-17	0.20229861252051506
1103	18-19	0.1757992191303117
1103	20-21	0.19876325170707076
1103	22-23	0.20158263772958662
1103	24-25	0.22465451299853356
1103	26-27	0.2385292250608586
1103	28-29	0.24310536648185632
1103	30-31	0.21583741129247613
1103	32-33	0.24457096341605933
1103	34-35	0.28180089801553265
1103	36-37	0.29315247999052474
1103	38-39	0.2699959251160564
1103	40-41	0.26323951446811833
1103	42-43	0.23304847592712719
1103	44-45	0.22161617668606226
1103	46-47	0.27958967489536946
1103	48-49	0.26160040445740407
1103	50-51	0.3119915792353041
1103	52-53	0.27629010004023513
1103	54-55	0.266104719618113
1103	56-57	0.29528337800964266
1103	58-59	0.279672967211944
1103	60-61	0.32890736466806914
1103	62-63	0.23341447253077519
1103	64-65	0.24294158214425465
1103	66-67	0.2757580827757735
1103	68-69	0.36007291276086306
1103	70-71	0.34211711619582275
1103	72-73	0.3177476095992162
1103	74-75	0.3467465864353514
1103	76-77	0.3146234900339522
1103	78-79	0.27308401728312504
1103	80-81	0.30515874847692714
1103	82-83	0.2897698607403214
1103	84-85	0.2292540527216218
1103	86-87	0.2493486123296691
1103	88-89	0.2677021141447682
1103	90-91	0.2648724515384302
1103	92-93	0.24210846362598915
1103	94-95	0.2311145805856043
1103	96-97	0.282228307018741
1103	98-99	0.2194020646063919
1103	100	0.26142367721785575
1104	1	0.1438983379991754
1104	2	0.1070883600686443
1104	3	0.14559285390957655
1104	4	0.09398310690740175
1104	5	0.10769695260560042
1104	6	0.09376349083945712
1104	7	0.09446446344238524
1104	8	0.09133013725361394
1104	9	0.1484609666613963
1104	10-11	0.13396240485774769
1104	12-13	0.11133473186679055
1104	14-15	0.204580986964487
1104	16-17	0.21024902629813624
1104	18-19	0.20531247932943586
1104	20-21	0.2077783463781273
1104	22-23	0.20946333115605853
1104	24-25	0.18298907683155363
1104	26-27	0.17688506148573424
1104	28-29	0.20333136363818483
1104	30-31	0.18272311168190214
1104	32-33	0.193051829122048
1104	34-35	0.20317612549072805
1104	36-37	0.20287016847846928
1104	38-39	0.2473370499256191
1104	40-41	0.24603177678025645
1104	42-43	0.2680432179900194
1104	44-45	0.24956127852794907
1104	46-47	0.2805212706771272
1104	48-49	0.3189955450970672
1104	50-51	0.2935086137950975
1104	52-53	0.265433508829247
1104	54-55	0.2773346659377438
1104	56-57	0.2995925640171322
1104	58-59	0.3286942110363853
1104	60-61	0.2919375630896113
1104	62-63	0.30153020429962396
1104	64-65	0.304841880799664
1104	66-67	0.25837281862835226
1104	68-69	0.26064098787419
1104	70-71	0.2561836533995887
1104	72-73	0.24770054286674537
1104	74-75	0.2018273650363014
1104	76-77	0.22521743765757662
1104	78-79	0.3002950723025606
1104	80-81	0.26623410102822476
1104	82-83	0.28407515597948674
1104	84-85	0.2687103238211037
1104	86-87	0.2791645037719732
1104	88-89	0.2787753688733048
1104	90-91	0.29615959052774343
1104	92-93	0.2744915835978361
1104	94-95	0.35098699282086443
1104	96-97	0.24995829744521103
1104	98-99	0.2822517833691265
1104	100	0.31285359431132775
1105	1	0.16952606902115264
1105	2	0.17124097757578483
1105	3	0.18259853287426608
1105	4	0.12987949112707042
1105	5	0.12345474125781664
1105	6	0.11072182028909339
1105	7	0.12218321460579062
1105	8	0.12804121405636693
1105	9	0.1478924149847387
1105	10-11	0.14464862499183795
1105	12-13	0.13668555436903773
1105	14-15	0.22330214364800582
1105	16-17	0.2232126385983051
1105	18-19	0.16782081398601179
1105	20-21	0.14949077873730943
1105	22-23	0.2017984966871822
1105	24-25	0.17397919342268864
1105	26-27	0.28009912668728987
1105	28-29	0.2839228693432787
1105	30-31	0.2795256471023251
1105	32-33	0.25978859401349297
1105	34-35	0.29078870091176157
1105	36-37	0.3176255760676696
1105	38-39	0.321368290557011
1105	40-41	0.28498905542079456
1105	42-43	0.27461078999446187
1105	44-45	0.31678547910669863
1105	46-47	0.38019591297946675
1105	48-49	0.3532335730760252
1105	50-51	0.3781771000082941
1105	52-53	0.376200222957749
1105	54-55	0.34612043611888765
1105	56-57	0.4232600870741976
1105	58-59	0.36551040800176793
1105	60-61	0.4106346672895498
1105	62-63	0.36343098880129787
1105	64-65	0.3591793277236448
1105	66-67	0.3134261334069137
1105	68-69	0.3915480639149749
1105	70-71	0.33357612746020493
1105	72-73	0.3518269025257794
1105	74-75	0.30967295243970483
1105	76-77	0.3311201212365802
1105	78-79	0.2952522936954054
1105	80-81	0.33437018844641386
1105	82-83	0.36197947751873727
1105	84-85	0.3249028857219045
1105	86-87	0.30585161897488433
1105	88-89	0.32974321864980993
1105	90-91	0.3061665982366506
1105	92-93	0.3602676284051469
1105	94-95	0.31006958471963486
1105	96-97	0.29729227771366595
1105	98-99	0.2634827542117222
1105	100	0.3327195688969127
1106	1	0.09861387961293389
1106	2	0.11538841988978277
1106	3	0.10421861790747755
1106	4	0.0780650761066326
1106	5	0.0963558056260112
1106	6	0.07048923197225321
1106	7	0.07869373384817635
1106	8	0.05375569131097535
1106	9	0.09415717114220001
1106	10-11	0.10063536160551934
1106	12-13	0.07178421263730428
1106	14-15	0.10854562653993582
1106	16-17	0.11648596856913684
1106	18-19	0.07816443115758887
1106	20-21	0.09762624262926778
1106	22-23	0.08698685213718704
1106	24-25	0.09951220471891986
1106	26-27	0.09813982662166154
1106	28-29	0.12455691548747438
1106	30-31	0.10980138789171434
1106	32-33	0.08144514774850364
1106	34-35	0.07560237102120482
1106	36-37	0.1031180819292814
1106	38-39	0.15717248513386295
1106	40-41	0.1478013087895107
1106	42-43	0.10684585674442104
1106	44-45	0.11379317536945877
1106	46-47	0.12875018430603546
1106	48-49	0.07387455618591332
1106	50-51	0.13382093012255325
1106	52-53	0.12503680663306227
1106	54-55	0.1261804433176792
1106	56-57	0.14348490978739648
1106	58-59	0.1713091473787287
1106	60-61	0.1378859083043693
1106	62-63	0.1070422764615202
1106	64-65	0.12244667020978994
1106	66-67	0.1746541633518106
1106	68-69	0.10910212606800229
1106	70-71	0.06825779093939843
1106	72-73	-0.017458552605056354
1106	74-75	-0.006946993048579486
1106	76-77	0.035167125724754555
1106	78-79	0.06693413815142435
1106	80-81	0.13745252024514087
1106	82-83	0.12931331762555942
1106	84-85	0.1273778086341153
1106	86-87	0.18069421372805294
1106	88-89	0.13621214774746448
1106	90-91	0.16357954161909305
1106	92-93	0.11408224022710556
1106	94-95	0.0883964294533115
1106	96-97	0.09249089619004991
1106	98-99	0.08605144268786802
1106	100	0.12649287254122754
1107	1	0.06935869606375178
1107	2	0.0475150782311502
1107	3	0.06772098857212683
1107	4	0.0738387435179817
1107	5	0.06392164598106831
1107	6	0.058672626901859815
1107	7	0.03829891649555606
1107	8	0.010882773634271814
1107	9	0.058320899771153734
1107	10-11	0.07690240130450121
1107	12-13	0.0620538947531557
1107	14-15	0.14740999028035873
1107	16-17	0.09934964209389818
1107	18-19	0.06752070814941646
1107	20-21	0.08265712331914443
1107	22-23	0.10535140714056723
1107	24-25	0.13450441556527437
1107	26-27	0.11204254390162305
1107	28-29	0.14622244570312404
1107	30-31	0.11685395707412027
1107	32-33	0.10910267318540434
1107	34-35	0.09408963850749785
1107	36-37	0.10389247482081032
1107	38-39	0.1202502681848685
1107	40-41	0.12075914937284438
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2216	42-43	-0.6697616076734576
2216	44-45	-0.6998446939452734
2216	46-47	-0.7478804031602664
2216	48-49	-0.7979317463239326
2216	50-51	-0.7854321571767144
2216	52-53	-0.7929058940906941
2216	54-55	-0.8412764922686335
2216	56-57	-0.9021195979392047
2216	58-59	-0.9490161128109023
2216	60-61	-0.9835461791615288
2216	62-63	-0.9220286680294763
2216	64-65	-0.8262093956762087
2216	66-67	-0.9284692996396373
2216	68-69	-0.9065558806246017
2216	70-71	-0.830407330970786
2216	72-73	-0.7881141363080744
2216	74-75	-0.8437612809593062
2216	76-77	-0.8087163349161557
2216	78-79	-0.9238967983541713
2216	80-81	-0.9245050339682521
2216	82-83	-0.9238498107518325
2216	84-85	-0.9371729682922592
2216	86-87	-0.9474113585845032
2216	88-89	-0.93937460212193
2216	90-91	-1.0183587212503156
2216	92-93	-1.0148761479454862
2216	94-95	-1.0475905000876011
2216	96-97	-0.99113138468838
2216	98-99	-1.0179491465574344
2216	100	-1.3166028951158069
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	14.0
8	66.0
9	114.0
10	200.0
11	220.0
12	172.0
13	188.0
14	319.0
15	498.0
16	875.0
17	1683.0
18	3198.0
19	4763.0
20	7064.0
21	10226.0
22	15311.0
23	19276.0
24	20129.0
25	19705.0
26	20639.0
27	24259.0
28	29387.0
29	36526.0
30	45827.0
31	58390.0
32	76095.0
33	101774.0
34	148650.0
35	242124.0
36	498277.0
37	1172790.0
38	1299097.0
39	326712.0
40	1119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.74947675852143	17.927447671073683	13.065960919834627	20.257114650570262
2	13.663498741271585	26.07642383862066	33.4121309039289	26.84794651617885
3	28.76670372167508	22.94899572804252	29.57976371280194	18.70453683748046
4	11.251868570201259	15.532217291928422	43.39017704859442	29.825737089275904
5	11.026469751024065	41.98413550095733	34.56272963646067	12.42666511155794
6	27.60192390258541	38.51157258799589	15.60319306091506	18.283310448503638
7	25.00879187248727	33.33275995034503	22.956439138740524	18.70200903842717
8	24.176700264496603	25.656481241908434	20.10305596190064	30.063762531694323
9	20.748684743985873	22.210762534322324	25.724116495093874	31.316436226597926
10-11	26.596255286169274	22.517462485847602	27.29277177199346	23.593510455989662
12-13	32.71632589823367	21.965760937212934	24.563590158557005	20.754323005996387
14-15	23.855751669768708	25.017927175352085	31.03507920866945	20.091241946209752
16-17	20.07932031229282	27.462887215408127	29.732717711572796	22.725074760726255
18-19	19.098095485878424	33.804295447796264	26.312622037911577	20.784987028413735
20-21	23.602756250049275	25.37501012378613	24.23414603146389	26.788087594700706
22-23	20.83471005471288	26.55669858271163	26.482385492020512	26.12620587055498
24-25	26.8456528163716	26.103158215126932	24.72256047812462	22.328628490376847
26-27	24.526212781796634	21.700404258607968	32.60243778380945	21.170945175785956
28-29	19.804877862314566	33.11441660936821	26.454074672586525	20.6266308557307
30-31	22.74078253225491	24.766856906541243	25.89259978156479	26.59976077963906
32-33	23.63007087603312	26.343732275616173	27.709850731719904	22.316346116630807
34-35	27.044154455361806	24.337373778157755	27.271145021790595	21.347326744689845
36-37	24.145514394797573	22.56621802790312	32.19019263741227	21.09807493988704
38-39	21.989937070856207	27.663749563987523	29.748904593301894	20.59740877185438
40-41	21.845458058558723	28.957259096754314	26.785983702563115	22.41129914212385
42-43	20.744851773851945	28.77991908095846	22.966541756208393	27.5086873889812
44-45	24.040969292501813	23.517409333245574	25.189434956852203	27.25218641740041
46-47	27.461829950089317	27.656266835687514	26.147120286634635	18.734782927588537
48-49	25.140700529402395	26.28207287590998	27.11520253780584	21.46202405688179
50-51	17.57390855091637	27.787226036499597	32.96105455229608	21.677810860287945
52-53	22.798830622141942	30.61996784485639	23.608693953822403	22.972507579179265
54-55	20.247506703898868	25.21150968171054	26.91844752419233	27.622536090198256
56-57	25.618385744074523	22.75354209710497	26.539233037784282	25.088839121036226
58-59	30.356843572332444	23.752670930279923	24.43732023819436	21.453165259193273
60-61	23.564732334308022	26.25093385365537	31.460302230521553	18.72403158151506
62-63	20.58578961281875	26.790394178967926	29.47552106309905	23.148295145114275
64-65	19.318948263491766	33.30298647926399	25.168552265119292	22.20951299212495
66-67	23.58153565037038	25.47411559923436	23.29848726682465	27.645861483570616
68-69	20.89519913569056	26.61408163060519	25.918603534360457	26.57211569934379
70-71	26.583525668416424	26.08345644331587	24.098888086007797	23.234129802259904
72-73	24.55232765250749	21.84174442949595	31.811537640936542	21.794390277060018
74-75	20.452703616401415	32.65291567973753	25.45163804353176	21.442742660329294
76-77	22.642114391063245	24.971559859999132	25.058071880953154	27.32825386798447
78-79	23.739427889371782	26.270558590654424	26.949555613584387	23.04045790638941
80-81	26.712619303906298	24.31947543133008	26.429977495925698	22.53792776883792
82-83	23.858901335290483	22.877531756481204	31.32756652979527	21.936000378433043
84-85	21.899233649562092	27.826701162032574	28.462487484121468	21.811577704283867
86-87	21.787778634135478	29.08619996817726	25.850111546828884	23.27590985085838
88-89	20.75421436899142	28.620713958066553	22.609396800519868	28.015674872422164
90-91	23.71844813049805	23.49232037655945	24.79608484819816	27.99314664474434
92-93	26.977554700100605	27.54105837345219	25.63328313846688	19.848103787980325
94-95	24.104191259403773	26.291108245790955	26.892574623950622	22.712125870854653
96-97	17.70199549654476	27.421837576973846	32.387476332968994	22.4886905935124
98-99	22.70034749555925	29.938743306021863	23.72753194534202	23.63337725307687
100	22.14983744483541	20.84626639169583	24.410223869328966	32.5936722941398
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	349.0
1	222.0
2	262.0
3	434.0
4	280.5
5	157.0
6	328.5
7	776.5
8	1471.0
9	1871.0
10	1391.0
11	856.5
12	920.5
13	1050.5
14	1239.0
15	1596.0
16	1900.5
17	2157.5
18	2561.5
19	3072.0
20	3599.5
21	4386.5
22	5430.5
23	6620.0
24	8168.5
25	9991.0
26	12604.5
27	16009.0
28	19307.0
29	23651.5
30	28427.0
31	32570.0
32	37974.0
33	42548.5
34	46658.0
35	52860.0
36	59952.0
37	67215.0
38	70321.0
39	70490.5
40	71267.5
41	72245.5
42	74783.0
43	79052.0
44	84187.0
45	92922.5
46	111418.5
47	292483.5
48	680698.0
49	676142.5
50	333595.0
51	173390.0
52	116958.5
53	99023.0
54	91975.5
55	81771.0
56	72923.0
57	63893.5
58	54649.0
59	50081.0
60	42147.0
61	31682.5
62	24651.0
63	19831.0
64	14485.0
65	9432.0
66	6750.0
67	5073.5
68	3867.0
69	3107.5
70	2317.0
71	1772.0
72	1519.0
73	1338.5
74	992.5
75	641.0
76	408.0
77	266.5
78	183.5
79	100.0
80	59.5
81	39.0
82	22.5
83	8.0
84	8.0
85	7.5
86	3.5
87	2.5
88	2.5
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005709934832680991
2	0.09761838379219469
3	4.3003693300526296E-4
4	0.0
5	0.03889445149625378
6	0.0056382620105134475
7	7.167282216754382E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	5.9727351806286515E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.3003693300526296E-4
24-25	0.0
26-27	0.0
28-29	2.389094072251461E-5
30-31	1.5529111469634495E-4
32-33	0.001648474909853508
34-35	0.0012064925064869875
36-37	6.092189884241225E-4
38-39	6.450553995078944E-4
40-41	0.0014215109729896192
42-43	5.256006958953214E-4
44-45	3.703095811989764E-4
46-47	2.3890940722514606E-4
48-49	5.733825773403505E-4
50-51	5.853280477016079E-4
52-53	0.0010153649807068707
54-55	0.0015051292655184202
56-57	7.406191623979528E-4
58-59	0.002711621772005408
60-61	0.001767929613466081
62-63	8.959102770942977E-4
64-65	0.004539278737277775
66-67	0.004383987622581431
68-69	0.0035597501676546766
70-71	0.0015887475580472217
72-73	0.00487375190739298
74-75	0.003464186404764618
76-77	0.0033805681122358166
78-79	6.68946340230409E-4
80-81	1.1945470361257305E-5
82-83	1.4334564433508764E-4
84-85	9.556376289005844E-5
86-87	2.389094072251461E-5
88-89	1.6723658505760224E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	2.8669128867017527E-4
98-99	5.256006958953214E-4
100	7.406191623979528E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4185687.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.52485689802136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.3404944071283	35.10312139038015
2	15.30682451824092	14.855229386215306
3	5.685856953237871	8.277161849954624
4	2.6451450620982273	5.134211424512972
5	1.415951710764456	3.435442706967688
6	0.8179964741239563	2.3815897109970607
7	0.5278759439622834	1.7930573248474424
8	0.3234534270166525	1.2556425007326126
9	0.21984723254366176	0.9601249948746492
>10	0.7039634656016449	4.945963328642966
>50	0.007043615113255081	0.23167970111801447
>100	0.0037231165529216566	0.36706607545480757
>500	6.688269925303093E-4	0.2134341331718987
>1k	1.8240736157592593E-4	0.18944713422073875
>5k	3.040122692932099E-4	0.9770940264737898
>10k+	6.688269924450618E-4	19.879734311435254
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	262301	6.266617642456304	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	67412	1.6105360959861548	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	62288	1.48811891572399	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	52087	1.2444074294136183	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	47221	1.1281541118578624	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	43854	1.0477133144451556	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	43504	1.0393514851922754	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	36871	0.8808828753798361	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	25023	0.597823009699483	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	21572	0.5153753732660851	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	11658	0.2785205869430753	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	8040	0.19208316340901746	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6894	0.1647041453410157	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	6534	0.15610340668091044	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6522	0.15581671539224026	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT	5127	0.12248885308433238	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.778188144502921E-5	0.0
10-11	0.0	0.0	0.0	2.9863675903143256E-4	0.0
12-13	0.0	0.0	0.0	4.7781881445029217E-4	0.0
14-15	0.0	0.0	0.0	6.808918105916664E-4	0.0
16-17	0.0	0.0	0.0	0.0013856745619058473	0.0
18-19	0.0	0.0	0.0	0.002197966546471344	0.0
20-21	0.0	0.0	0.0	0.0035597501676546766	0.0
22-23	0.0	0.0	0.0	0.0056382620105134475	0.0
24-25	0.0	0.0	0.0	0.007465918975785815	0.0
26-27	0.0	0.0	0.0	0.00969972193334093	0.0
28-29	0.0	0.0	0.0	0.015756075406498385	0.0
30-31	0.0	0.0	0.0	0.02703259942752528	0.0
32-33	0.0	0.0	0.0	0.06144749953830757	0.0
34-35	0.0	0.0	0.0	0.10520375747159308	0.0
36-37	0.0	0.0	0.0	0.15662900737680577	0.0
38-39	0.0	0.0	0.0	0.23127625166430266	0.0
40-41	0.0	0.0	0.0	0.3079661713835745	0.0
42-43	0.0	0.0	0.0	0.38966124318421325	0.0
44-45	0.0	0.0	0.0	0.49042128568141863	0.0
46-47	0.0	0.0	0.0	0.6164459979926832	0.0
48-49	0.0	0.0	0.0	0.7173254951935011	0.0
50-51	0.0	0.0	0.0	0.8238790908159163	0.0
52-53	0.0	0.0	0.0	0.9294531578687083	0.0
54-55	0.0	0.0	0.0	1.0596348938656903	0.0
56-57	0.0	0.0	0.0	1.248014961462718	0.0
58-59	0.0	0.0	0.0	1.3965210489938689	0.0
60-61	0.0	0.0	0.0	1.5419332597014541	0.0
62-63	0.0	0.0	0.0	1.6870468336500077	0.0
64-65	0.0	0.0	0.0	1.8302491323407604	0.0
66-67	0.0	0.0	0.0	2.006516492991473	0.0
68-69	0.0	0.0	0.0	2.2157414063688945	0.0
70-71	0.0	0.0	0.0	2.4185874385734047	0.0
72-73	0.0	0.0	0.0	2.5877711352998922	0.0
74-75	0.0	0.0	0.0	2.7884430918986536	1.1945470361257303E-5
76-77	0.0	0.0	0.0	2.9698111683936235	2.3890940722514606E-5
78-79	0.0	0.0	0.0	3.266369415582197	2.3890940722514606E-5
80-81	0.0	0.0	0.0	3.5548883612176447	2.3890940722514606E-5
82-83	0.0	0.0	0.0	3.774039960465271	2.3890940722514606E-5
84-85	0.0	0.0	0.0	4.009007362471202	2.3890940722514606E-5
86-87	0.0	0.0	0.0	4.243281927196181	2.3890940722514606E-5
88	0.0	2.3890940722514606E-5	0.0	4.447203051733204	2.3890940722514606E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAAC	365	0.0	27.077204	1
GAGTACG	3575	0.0	25.933884	1
GTACGCA	3655	0.0	25.212753	3
TACGCAG	3670	0.0	25.109404	4
AGTACGC	3820	0.0	24.144785	2
GCTTATG	1475	0.0	23.611145	1
CGCAGTG	3910	0.0	23.55859	6
GTATAAG	1265	0.0	23.066334	1
CTCGTCG	1190	0.0	21.721294	9
GTACCCG	330	1.8189894E-12	21.392162	1
CGACGAG	870	0.0	21.33771	24-25
GAGTACT	8470	0.0	21.251293	12-13
CAATGCG	945	0.0	20.88755	18-19
GCATCGT	1820	0.0	20.658016	8
TGCGACG	925	0.0	20.577057	22-23
TAGGCAT	2230	0.0	20.240107	5
TAGGACT	1750	0.0	20.149748	4
TACTAGG	2315	0.0	19.920752	2
CTAGGCA	2440	0.0	19.654263	4
CTTTGCG	1365	0.0	19.650465	2
>>END_MODULE