FastQCFastQC Report
Mon 16 Jan 2017
ERR1146533_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146533_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4533175
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1508093.32678531051636No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1208692.6663210663607737No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT448720.9898581016616389No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG307720.6788178263579059No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG206130.4547144109812659No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA187820.41432329437976695No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA140920.31086379855178764No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC92520.20409536362483247No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG88010.19414648673391166No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC80730.17808710230688204No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC75830.16727790125022748No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG66750.1472477899044268No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA66400.14647570411466576No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG66190.14601245264080914No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA54190.11954093984900209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG36750.026.0865862
GTACCCG6000.025.8607981
GTACTAG16650.025.1336171
TCTAACG6950.023.6661512
ACCTGGG40750.022.1420573
GTGATCG10050.021.9759398
TAGGCAT21400.021.7400745
CATGGGG75800.021.2031964
GGGCTAT23050.020.9988886
TGATCGC10900.020.262229
GCATCGT16850.020.0792858
TTTAGGG23100.019.936942
GTACCTG51200.019.4690671
CTAACGC8450.019.465063
CTAGGCA24900.019.4393734
GGGCACC20450.019.30729394
CTATTGA24650.019.253969
TAACGCC8350.019.1353684
TATACTC79750.019.092324
GGCTATT25150.019.057187
Produced by FastQC (version 0.11.5)