##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146533_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4533175
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.559945071610958	31.0	31.0	34.0	30.0	34.0
2	31.818213503780463	33.0	31.0	34.0	30.0	34.0
3	31.96112283333425	34.0	31.0	34.0	30.0	34.0
4	35.391883613582095	37.0	35.0	37.0	33.0	37.0
5	35.39254915153287	37.0	35.0	37.0	33.0	37.0
6	35.53082067204553	37.0	35.0	37.0	33.0	37.0
7	35.51873157334539	37.0	35.0	37.0	33.0	37.0
8	35.47264533136268	37.0	36.0	37.0	33.0	37.0
9	37.14121250558384	39.0	38.0	39.0	34.0	39.0
10-11	37.130901410159545	39.0	38.0	39.0	34.0	39.0
12-13	37.10073138583884	39.0	38.0	39.0	34.0	39.0
14-15	38.41685176504326	40.0	38.5	41.0	34.0	41.0
16-17	38.43182339089049	40.0	38.5	41.0	34.0	41.0
18-19	38.57248561990217	40.5	39.0	41.0	34.0	41.0
20-21	38.63113755811324	41.0	39.0	41.0	34.0	41.0
22-23	38.63823269562724	41.0	39.0	41.0	34.0	41.0
24-25	38.644538871762066	41.0	39.0	41.0	34.0	41.0
26-27	38.513905706265476	40.5	38.5	41.0	34.0	41.0
28-29	38.398622488653096	40.0	38.0	41.0	33.5	41.0
30-31	38.38398749221021	40.0	38.0	41.0	34.0	41.0
32-33	38.384812300429616	40.0	38.0	41.0	33.0	41.0
34-35	38.27946869467867	40.0	38.0	41.0	33.0	41.0
36-37	38.0498913013506	40.0	38.0	41.0	33.0	41.0
38-39	37.90245931383633	40.0	38.0	41.0	32.5	41.0
40-41	37.820635647200916	40.0	38.0	41.0	32.0	41.0
42-43	37.752431243002974	40.0	38.0	41.0	32.0	41.0
44-45	37.69295085673948	40.0	37.0	41.0	32.0	41.0
46-47	37.534548081642555	40.0	37.0	41.0	31.0	41.0
48-49	37.34582329162232	40.0	37.0	41.0	31.0	41.0
50-51	37.115509990238635	40.0	36.0	41.0	30.0	41.0
52-53	36.81622372840228	39.0	35.5	41.0	30.0	41.0
54-55	36.647274371715184	39.0	35.0	41.0	29.5	41.0
56-57	36.49766223452657	39.0	35.0	41.0	29.0	41.0
58-59	36.272523452105865	39.0	35.0	40.5	29.0	41.0
60-61	35.933516023537585	38.0	35.0	40.0	28.0	41.0
62-63	35.593889382165926	37.5	34.0	40.0	28.0	41.0
64-65	35.11218594031777	37.0	34.0	40.0	27.5	41.0
66-67	34.741694287116644	36.0	33.0	39.0	26.0	41.0
68-69	34.58912638492889	36.0	34.0	39.0	27.0	41.0
70-71	34.22170884644868	35.5	33.0	38.5	26.0	40.5
72-73	33.75110590259586	35.0	33.0	37.0	26.0	40.0
74-75	33.401113347708836	35.0	32.5	37.0	26.0	39.0
76-77	32.974454218070115	35.0	32.0	36.5	25.5	39.0
78-79	32.67499644289047	35.0	32.0	36.0	25.5	39.0
80-81	32.448164807226725	35.0	32.0	36.0	25.0	37.5
82-83	32.261208865750824	35.0	31.5	35.0	25.0	37.0
84-85	32.072064612550804	35.0	31.0	35.0	25.0	37.0
86-87	31.79979914739669	35.0	31.0	35.0	24.0	36.0
88-89	31.500024486149332	34.5	31.0	35.0	23.5	36.0
90-91	31.202667446105654	34.0	30.0	35.0	21.5	36.0
92-93	31.2652599998897	34.0	30.5	35.0	22.5	35.5
94-95	31.387296762203093	34.0	31.0	35.0	23.0	35.0
96-97	31.23431965454676	34.0	31.0	35.0	23.0	35.0
98-99	31.118570758905182	34.0	31.0	35.0	20.5	35.0
100	29.74167796301709	34.0	28.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19559158112099695
1101	2	0.2875668565995788
1101	3	0.30520667319565575
1101	4	0.29619723901750206
1101	5	0.212849143842206
1101	6	0.2580721117822691
1101	7	0.27039890789189513
1101	8	0.34031133161575156
1101	9	0.4339190421552104
1101	10-11	0.4618166888756292
1101	12-13	0.4703494446686278
1101	14-15	0.55172363946415
1101	16-17	0.547933900545992
1101	18-19	0.486874453361807
1101	20-21	0.4569311856125964
1101	22-23	0.44845256631850106
1101	24-25	0.4172462045255543
1101	26-27	0.43040109784496394
1101	28-29	0.47230091234455074
1101	30-31	0.4483160886189239
1101	32-33	0.469039123451104
1101	34-35	0.4917129638903006
1101	36-37	0.5179705890376383
1101	38-39	0.515017991863111
1101	40-41	0.4993558908293849
1101	42-43	0.5179953657717746
1101	44-45	0.45263635589832774
1101	46-47	0.4266182438893793
1101	48-49	0.5007224180738703
1101	50-51	0.5331020558652213
1101	52-53	0.5061836214539
1101	54-55	0.5742445455509397
1101	56-57	0.49101474013124147
1101	58-59	0.5493379207190685
1101	60-61	0.5669532637323584
1101	62-63	0.5883666542287216
1101	64-65	0.5917685069321621
1101	66-67	0.571290838050281
1101	68-69	0.5381232332062424
1101	70-71	0.4657488335580027
1101	72-73	0.4639180041987032
1101	74-75	0.4548285436904038
1101	76-77	0.5191165393137069
1101	78-79	0.5763318859493225
1101	80-81	0.44726398905380904
1101	82-83	0.46785851218491814
1101	84-85	0.4437226254216071
1101	86-87	0.4261448734857183
1101	88-89	0.39927550123427835
1101	90-91	0.3996472144818952
1101	92-93	0.4015206347544975
1101	94-95	0.3221968657587695
1101	96-97	0.384943099770247
1101	98-99	0.29385594362735645
1101	100	0.3032882552792806
1102	1	0.1758661557794774
1102	2	0.2768923223709798
1102	3	0.2974774790456749
1102	4	0.2702242643018593
1102	5	0.22953405042652975
1102	6	0.23087084707052696
1102	7	0.24998751061545477
1102	8	0.2917274230569902
1102	9	0.37163930803976797
1102	10-11	0.36240771448761677
1102	12-13	0.3526474012750498
1102	14-15	0.39391630120835686
1102	16-17	0.3981110571984203
1102	18-19	0.3477528796635312
1102	20-21	0.37118165860155017
1102	22-23	0.3754139819220015
1102	24-25	0.41342222612069435
1102	26-27	0.43240853438718574
1102	28-29	0.44159160028569744
1102	30-31	0.4293175298047771
1102	32-33	0.43069488102052134
1102	34-35	0.4000016413742813
1102	36-37	0.43901758064204444
1102	38-39	0.37595993838762354
1102	40-41	0.4008446514966906
1102	42-43	0.38406791359201264
1102	44-45	0.34641416233329636
1102	46-47	0.35542123767559985
1102	48-49	0.3197638610256277
1102	50-51	0.3750684903505217
1102	52-53	0.3836561178073623
1102	54-55	0.44909817602066937
1102	56-57	0.374570699211084
1102	58-59	0.36166980415321603
1102	60-61	0.33871289008126126
1102	62-63	0.3613964091109523
1102	64-65	0.3108219742882241
1102	66-67	0.32460289341598525
1102	68-69	0.35317254488170846
1102	70-71	0.3218928249283124
1102	72-73	0.32615572551018346
1102	74-75	0.2845714015137233
1102	76-77	0.2760714954304362
1102	78-79	0.3259989929299252
1102	80-81	0.26122725595085683
1102	82-83	0.30604203712451294
1102	84-85	0.2913014070531972
1102	86-87	0.24563270066423826
1102	88-89	0.2708802189831694
1102	90-91	0.343158697788013
1102	92-93	0.38203404196530855
1102	94-95	0.31541370759254406
1102	96-97	0.3382737145426056
1102	98-99	0.24245052602327988
1102	100	0.17559428043526282
1103	1	0.13500417184937774
1103	2	0.22107982300728324
1103	3	0.27171467917734304
1103	4	0.24921908677618632
1103	5	0.21329138770550315
1103	6	0.2514749275535024
1103	7	0.2088768595277415
1103	8	0.20320205634858013
1103	9	0.28935545022319786
1103	10-11	0.2907845737645687
1103	12-13	0.25274950667395757
1103	14-15	0.3716346435856792
1103	16-17	0.325332503602489
1103	18-19	0.27522657066128886
1103	20-21	0.28957151825293437
1103	22-23	0.3018532848508002
1103	24-25	0.3403892362212275
1103	26-27	0.3732992085062321
1103	28-29	0.41639581696576755
1103	30-31	0.38710406688059606
1103	32-33	0.39263579704777385
1103	34-35	0.4069867903483342
1103	36-37	0.38747901446711097
1103	38-39	0.33179681522088345
1103	40-41	0.38016205124080926
1103	42-43	0.38263454948990017
1103	44-45	0.3903050468564899
1103	46-47	0.35454161260221895
1103	48-49	0.3424127926589833
1103	50-51	0.3631869484501138
1103	52-53	0.4315538297188368
1103	54-55	0.4027975418671019
1103	56-57	0.43454886129693904
1103	58-59	0.5051158139969658
1103	60-61	0.4711094442043233
1103	62-63	0.4640436976689273
1103	64-65	0.5039124322339816
1103	66-67	0.5298772078208671
1103	68-69	0.46032478698673884
1103	70-71	0.4027792344041927
1103	72-73	0.38389922023254996
1103	74-75	0.44434690820876455
1103	76-77	0.5157609570107553
1103	78-79	0.5119284136511126
1103	80-81	0.4630023995290671
1103	82-83	0.3757806043175336
1103	84-85	0.3985863960748546
1103	86-87	0.40140429282408974
1103	88-89	0.3647371673538409
1103	90-91	0.45185022721122436
1103	92-93	0.46212172167137666
1103	94-95	0.41472385631207587
1103	96-97	0.38954562739909093
1103	98-99	0.39015112544885255
1103	100	0.4465205564063197
1104	1	0.11439694110282872
1104	2	0.1701967392294641
1104	3	0.16877224264357693
1104	4	0.21412664224101974
1104	5	0.14900847706036302
1104	6	0.1718790991186694
1104	7	0.20096586787062165
1104	8	0.2405158347614318
1104	9	0.35079487079574534
1104	10-11	0.29270057858305165
1104	12-13	0.32100342767555645
1104	14-15	0.3748678081965551
1104	16-17	0.35375078724523235
1104	18-19	0.33995507114830303
1104	20-21	0.3433464470278622
1104	22-23	0.38388363410251003
1104	24-25	0.30754592570763606
1104	26-27	0.292739195477175
1104	28-29	0.2993305923153997
1104	30-31	0.3466541487216972
1104	32-33	0.32405323581815537
1104	34-35	0.33654713225387667
1104	36-37	0.34223384712442595
1104	38-39	0.3424993193444408
1104	40-41	0.3512386397709548
1104	42-43	0.3125220352984499
1104	44-45	0.2872505434536876
1104	46-47	0.31178451876152735
1104	48-49	0.3179398455854212
1104	50-51	0.3603603904765009
1104	52-53	0.3061447817091718
1104	54-55	0.3171500237505427
1104	56-57	0.2821724136418524
1104	58-59	0.3838730861365889
1104	60-61	0.3717296788028861
1104	62-63	0.34410040996247915
1104	64-65	0.33517343416061607
1104	66-67	0.30116227380407423
1104	68-69	0.28462709000421427
1104	70-71	0.31157987791845443
1104	72-73	0.27043242100723575
1104	74-75	0.30239055301711204
1104	76-77	0.2984372303990952
1104	78-79	0.26059573933081737
1104	80-81	0.24727958318705134
1104	82-83	0.29616823755346644
1104	84-85	0.33496155342524503
1104	86-87	0.3203656132946904
1104	88-89	0.2506813673460293
1104	90-91	0.3584299306175538
1104	92-93	0.347614110259741
1104	94-95	0.32864303396964445
1104	96-97	0.3254697608851451
1104	98-99	0.3248527613888825
1104	100	0.2910353753793409
1105	1	0.09250613908016803
1105	2	0.19117652517116213
1105	3	0.2570601995867179
1105	4	0.1901210298400926
1105	5	0.18663902922077824
1105	6	0.18056863465963602
1105	7	0.18134837270425663
1105	8	0.22432277792317024
1105	9	0.24980315020471267
1105	10-11	0.2873087349879171
1105	12-13	0.2709261606425173
1105	14-15	0.3377451729013572
1105	16-17	0.316481000839552
1105	18-19	0.25559726805850147
1105	20-21	0.2715868279341791
1105	22-23	0.25689391910153603
1105	24-25	0.25866788095811444
1105	26-27	0.33510720059241805
1105	28-29	0.31920014090862736
1105	30-31	0.2840193378151028
1105	32-33	0.31934533161911105
1105	34-35	0.3317831785524561
1105	36-37	0.3244190800617517
1105	38-39	0.3511151946632509
1105	40-41	0.34081978234768684
1105	42-43	0.3720861462771339
1105	44-45	0.3083622404598998
1105	46-47	0.28282826209613177
1105	48-49	0.29773840265786333
1105	50-51	0.24716384840906613
1105	52-53	0.29188348536376907
1105	54-55	0.2940468760714481
1105	56-57	0.2921529504098217
1105	58-59	0.29434238811200686
1105	60-61	0.30005752063966185
1105	62-63	0.31045013447324266
1105	64-65	0.3548994723210157
1105	66-67	0.3322898136847314
1105	68-69	0.36140965891377874
1105	70-71	0.3145825903079711
1105	72-73	0.32194597770545386
1105	74-75	0.39513800141008204
1105	76-77	0.308026944655019
1105	78-79	0.24785573824031104
1105	80-81	0.29223255409853977
1105	82-83	0.33421187962642307
1105	84-85	0.33290414722010553
1105	86-87	0.36043033946324243
1105	88-89	0.3616402473313407
1105	90-91	0.33162675377916884
1105	92-93	0.38511960937632495
1105	94-95	0.2551268302447731
1105	96-97	0.23127738680094367
1105	98-99	0.30122112278252544
1105	100	0.32466998748799725
1106	1	0.08797370454683318
1106	2	0.13457567149421124
1106	3	0.1270350204477282
1106	4	0.12539992348334295
1106	5	0.08030287012733339
1106	6	0.10949718332330605
1106	7	0.044583302950513826
1106	8	0.043880594284033236
1106	9	0.1731702675794935
1106	10-11	0.14446518173821943
1106	12-13	0.0926546394537553
1106	14-15	0.18225759927999974
1106	16-17	0.1515193445640861
1106	18-19	0.1581950740579856
1106	20-21	0.1610604589880822
1106	22-23	0.17875320250674065
1106	24-25	0.15127094669036012
1106	26-27	0.15649844682366165
1106	28-29	0.16329647800559144
1106	30-31	0.14732273610907498
1106	32-33	0.12556370024775987
1106	34-35	0.1406452111722345
1106	36-37	0.19155590323401128
1106	38-39	0.19391340068715834
1106	40-41	0.2110608023558882
1106	42-43	0.1551748065863663
1106	44-45	0.1284523626688383
1106	46-47	0.09754808576033724
1106	48-49	0.17745590892422314
1106	50-51	0.21580394563669358
1106	52-53	0.13549833897111085
1106	54-55	0.18330344319146263
1106	56-57	0.054122158947841115
1106	58-59	0.11020599218036153
1106	60-61	0.09615519090484526
1106	62-63	0.13256167612222924
1106	64-65	0.14592448648956946
1106	66-67	0.14179222188797524
1106	68-69	0.18128992800094323
1106	70-71	0.118107524163797
1106	72-73	0.13910862575417582
1106	74-75	0.15467594135224516
1106	76-77	0.16269935907850197
1106	78-79	0.16683531911555605
1106	80-81	0.07914183547224951
1106	82-83	0.12930881859507792
1106	84-85	0.14455782726037825
1106	86-87	0.049081790022100336
1106	88-89	0.1310930423738519
1106	90-91	0.13071124873504658
1106	92-93	0.15454378073036779
1106	94-95	0.11102437782446017
1106	96-97	0.06963180274619774
1106	98-99	0.0647491263180413
1106	100	0.017628084025890445
1107	1	0.005947062956622062
1107	2	0.07630765932955441
1107	3	0.12287931668057439
1107	4	0.10440429361444359
1107	5	0.08546992485648985
1107	6	0.07399255180018116
1107	7	0.07447766729556804
1107	8	0.11973352665166459
1107	9	0.15363778894976576
1107	10-11	0.147990418145028
1107	12-13	0.06945307829392533
1107	14-15	0.18529842849923028
1107	16-17	0.13442701682249947
1107	18-19	0.17582253084778188
1107	20-21	0.172676905645325
1107	22-23	0.1898443156073668
1107	24-25	0.16579531250607715
1107	26-27	0.2141301545884602
1107	28-29	0.23859737403812176
1107	30-31	0.22192486470965633
1107	32-33	0.22317476770407296
1107	34-35	0.22472991238557682
1107	36-37	0.23913663150331388
1107	38-39	0.1685706524103594
1107	40-41	0.20873732516569987
1107	42-43	0.17591930957117086
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2216	76-77	-1.3801936948973221
2216	78-79	-1.3212630443758542
2216	80-81	-1.2872855688413125
2216	82-83	-1.2024180927745647
2216	84-85	-1.273455686621368
2216	86-87	-1.4172676796269101
2216	88-89	-1.4048590280120763
2216	90-91	-1.406675017964318
2216	92-93	-1.34523722838712
2216	94-95	-1.2308967317779995
2216	96-97	-1.2792747366003603
2216	98-99	-1.3835214843091919
2216	100	-1.4273832329045355
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	139.0
1	61.0
2	53.0
3	62.0
4	50.0
5	78.0
6	120.0
7	456.0
8	1445.0
9	2204.0
10	2404.0
11	3361.0
12	4913.0
13	6115.0
14	7579.0
15	8802.0
16	9663.0
17	10842.0
18	12065.0
19	14009.0
20	16404.0
21	19362.0
22	22649.0
23	27654.0
24	33527.0
25	42068.0
26	48047.0
27	49449.0
28	55076.0
29	68723.0
30	81105.0
31	96236.0
32	123399.0
33	168229.0
34	250048.0
35	384395.0
36	635697.0
37	1021689.0
38	997437.0
39	306940.0
40	620.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.08648968885114	16.883209474761372	12.114154134392363	21.916146701995128
2	12.961955064940051	22.417979142320537	39.240597089737975	25.379468703001447
3	23.617772710304788	27.099363457538388	29.846300543704157	19.436563288452664
4	11.519524029931684	15.041513686478014	41.705381446292094	31.73358083729821
5	12.842334247820508	40.82817900121233	33.99178616223157	12.337700588735592
6	26.633636236075287	37.244395475609075	19.641538123473833	16.48043016484181
7	25.962920198701354	35.34487056571823	21.105378869869497	17.586830365710913
8	21.607286804535544	31.768119612451702	21.592616303978065	25.031977279034685
9	24.680177020044916	21.415211722503873	22.167723403973685	31.736887853477526
10-11	23.452630218117264	23.915938296479666	28.619956993820868	24.011474491582202
12-13	31.360259331071788	21.62778825796241	25.542639104871878	21.469313306093923
14-15	26.70130905608332	23.73669210150653	27.99633374725205	21.5656650951581
16-17	21.006512438141325	26.91785335530988	30.455592690555388	21.62004151599341
18-19	19.89509367927946	32.44916063030273	27.921457481304074	19.734288209113735
20-21	21.85406912183203	29.507430366955546	23.8592859263093	24.779214584903123
22-23	20.962478438895516	28.231044151979894	22.585415505288164	28.221061903836425
24-25	23.662302147830363	28.116710085367437	24.670510190507684	23.550477576294515
26-27	26.182986838463794	24.02363938575453	27.30337362466574	22.490000151115936
28-29	22.021251366403515	26.363519039199097	29.270828622389494	22.344400972007893
30-31	21.764409282539845	30.220948822088328	26.484615894579587	21.530026000792233
32-33	21.7914753800343	29.40472563380232	24.86710409706569	23.936694889097694
34-35	23.565921026127754	26.155078759205786	25.466656870992516	24.81234334367395
36-37	25.630260258842352	25.417533217984378	27.825266187355126	21.126940335818144
38-39	27.17828696246939	23.351245504489995	28.637303355912007	20.83316417712861
40-41	22.38109579520445	26.08560727661851	30.640543209059366	20.89275371911767
42-43	21.0076848530637	29.453068733848305	26.714208990580822	22.825037422507165
44-45	21.69880359898925	29.744464966939244	24.449028753717965	24.107702680353544
46-47	22.261918527162024	27.603586503104303	23.16449120961179	26.97000376012188
48-49	24.02200901739043	26.794143815726063	24.909305318459698	24.274541848423812
50-51	25.41858497309985	27.699037429166513	26.08705870125262	20.795318896481017
52-53	21.950849762276636	28.102010751724837	28.02623408502433	21.920905400974195
54-55	19.97103806065346	26.994220080505983	29.08804648033765	23.94669537850291
56-57	23.54135471918382	29.005538090787613	24.644150908836117	22.80895628119245
58-59	22.61087760108548	27.09033831227734	25.881652217463753	24.417131869173428
60-61	22.606708277505685	25.14329081218595	28.16913374945644	24.08086716085193
62-63	27.134535005167017	24.48100915542329	25.873145893829395	22.511309945580297
64-65	24.511958110367654	26.53704810762465	28.094315668868354	20.856678113139345
66-67	22.073264992102377	26.11456323641943	28.713633838343437	23.098537933134757
68-69	20.92770210498521	30.08931514243825	26.93321110242955	22.049771650146994
70-71	22.157485423737793	28.872717562575307	24.116034109115624	24.853762904571276
72-73	21.96087981089691	27.01177193891687	24.3609037323542	26.666444517832023
74-75	22.944839499334364	28.69410834588014	25.387292026082637	22.97376012870287
76-77	25.219433486608782	24.86919836531197	26.502639535444413	23.408728612634835
78-79	22.764658150280013	25.426538386082427	28.91300543471904	22.895798028918524
80-81	22.343134767783436	29.663109507313806	26.895040056250117	21.09871566865264
82-83	22.20101454778055	27.921690937978365	26.01774493126098	23.8595495829801
84-85	22.99165087277503	26.369434397909853	25.590760692005816	25.048154037309295
86-87	24.643747238842494	26.640750955399113	26.929378804108207	21.786123001650186
88-89	26.299999006998455	23.797006210673004	27.320550828990793	22.582443953337755
90-91	22.923456919802156	25.20793897455611	29.338660054753067	22.529944050888666
92-93	22.08976392305382	29.087917527211005	27.151243144976828	21.671075404758348
94-95	21.638350932669752	29.784600629367674	25.31459832212021	23.26245011584236
96-97	21.178909668268563	27.2610406583543	24.469511613165544	27.090538060211593
98-99	23.37366428835362	27.55895755545888	24.470909292919778	24.596468863267724
100	21.842564877141378	30.123779881659416	25.940110352649643	22.09354488854956
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2013.0
1	1457.0
2	902.0
3	881.5
4	756.5
5	587.0
6	470.5
7	459.0
8	489.5
9	483.5
10	432.0
11	363.0
12	373.0
13	412.0
14	463.5
15	537.0
16	606.5
17	726.5
18	891.0
19	1137.0
20	1592.0
21	2206.5
22	3221.0
23	4444.0
24	5809.5
25	8168.5
26	11882.0
27	16295.0
28	20685.5
29	27119.0
30	35101.5
31	42317.5
32	50816.0
33	58988.5
34	66824.0
35	74750.0
36	83648.0
37	93872.5
38	98009.5
39	97603.5
40	97757.0
41	96497.5
42	95723.5
43	100554.5
44	111757.5
45	127747.5
46	153223.0
47	242201.0
48	649749.0
49	656130.5
50	277838.5
51	189794.5
52	133516.5
53	111741.0
54	102691.0
55	91099.0
56	82156.5
57	72423.5
58	62120.5
59	56966.5
60	48675.0
61	38278.0
62	30048.0
63	23145.5
64	16816.0
65	11150.5
66	8000.0
67	6042.0
68	4713.0
69	3901.0
70	3094.0
71	2468.0
72	2000.0
73	1708.5
74	1320.0
75	901.0
76	698.0
77	537.0
78	355.5
79	231.0
80	160.5
81	110.5
82	75.0
83	57.5
84	46.0
85	32.0
86	23.0
87	16.5
88	11.5
89	10.5
90	9.0
91	7.5
92	6.5
93	6.5
94	6.0
95	4.5
96	6.0
97	6.0
98	5.0
99	5.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06042122794729963
2	0.010059174860886685
3	0.01241955141815615
4	0.009992996078907168
5	0.012794564516040082
6	0.012948981673992289
7	0.0031104027530373304
8	0.005934030784163417
9	0.007831155867576257
10-11	0.0074561427696923244
12-13	0.006220805506074661
14-15	0.010621694507712585
16-17	0.010235651612832066
18-19	0.008139990183480673
20-21	0.007136278656791322
22-23	0.0028236280311260873
24-25	0.004014846106757405
26-27	0.005106796009419446
28-29	0.006397282258020041
30-31	0.00945253602607444
32-33	0.010853320244640897
34-35	0.012397491824162976
36-37	0.01855211854825812
38-39	0.019302144744025987
40-41	0.01875065489419667
42-43	0.02521411593419623
44-45	0.028489965642182355
46-47	0.031037848748393788
48-49	0.03280261626784759
50-51	0.033276897538700806
52-53	0.03169963656818896
54-55	0.037732935525321656
56-57	0.040369057007505774
58-59	0.03806382943521924
60-61	0.03846090212709635
62-63	0.03406001312545843
64-65	0.03635421090074837
66-67	0.03854914050306904
68-69	0.04104187462429754
70-71	0.04047935497747164
72-73	0.03702702851754013
74-75	0.039872716142659394
76-77	0.039497703044775456
78-79	0.03869252786402466
80-81	0.04377726427945094
82-83	0.039630060608734495
84-85	0.03581375084791564
86-87	0.03443502622334236
88-89	0.032207007230031934
90-91	0.03434678784736967
92-93	0.03626597252477568
94-95	0.03974035857870036
96-97	0.03468871155426384
98-99	0.03224009662102169
100	0.02955985595085122
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4533175.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.30815485186455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.6238666160053	35.80948861043146
2	14.060678470062069	13.866122226481966
3	5.592812352772986	8.273137726438538
4	2.7875007769920535	5.497860798464677
5	1.5613338512240287	3.8493245655806225
6	0.9313157585760494	2.7552876983909727
7	0.5936690262843499	2.049090699514906
8	0.4234804900974535	1.670483326597498
9	0.29383832307777247	1.303976298215791
>10	1.1001597358008905	8.38355519942285
>50	0.022342719697102147	0.754499346555485
>100	0.007323754371234593	0.6395099220930881
>500	3.227163536134028E-4	0.09690741693687166
>1k	3.8725962430673466E-4	0.415317501129412
>5k	5.163461657423129E-4	1.8796538123912137
>10k+	4.518028950245238E-4	12.755784851354676
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	150809	3.32678531051636	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	120869	2.6663210663607737	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	44872	0.9898581016616389	No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG	30772	0.6788178263579059	No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG	20613	0.4547144109812659	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	18782	0.41432329437976695	No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA	14092	0.31086379855178764	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	9252	0.20409536362483247	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	8801	0.19414648673391166	No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC	8073	0.17808710230688204	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	7583	0.16727790125022748	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	6675	0.1472477899044268	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	6640	0.14647570411466576	No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG	6619	0.14601245264080914	No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	5419	0.11954093984900209	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.617878197951767E-5
2	0.0	0.0	0.0	0.0	6.617878197951767E-5
3	0.0	0.0	0.0	0.0	6.617878197951767E-5
4	0.0	0.0	0.0	0.0	6.617878197951767E-5
5	0.0	0.0	0.0	0.0	8.823837597269023E-5
6	0.0	0.0	0.0	0.0	1.7647675194538046E-4
7	0.0	0.0	0.0	0.0	1.7647675194538046E-4
8	0.0	0.0	0.0	0.0	1.7647675194538046E-4
9	0.0	0.0	0.0	0.0	1.7647675194538046E-4
10-11	0.0	0.0	0.0	5.5148984982931394E-5	1.8750654894196674E-4
12-13	0.0	0.0	0.0	7.720857897610395E-5	6.507580227985904E-4
14-15	0.0	0.0	0.0	2.7574492491465693E-4	8.38264571740557E-4
16-17	0.0	0.0	0.0	4.853110678497962E-4	9.265029477132473E-4
18-19	0.0	0.0	0.0	7.500261957678669E-4	0.0010147413236859376
20-21	0.0	0.0	0.0	0.0012463670606142495	0.0011801882786347316
22-23	0.0	0.0	0.0	0.0024486149332421537	0.0012022478726279044
24-25	0.0	0.0	0.0	0.0038383693548120247	0.0012132776696244906
26-27	0.0	0.0	0.0	0.00539357073133069	0.001279456451604008
28-29	0.0	0.0	0.0	0.008923105770238299	0.0013235756395903533
30-31	0.0	0.0	0.0	0.01703000656272921	0.0013346054365869398
32-33	0.0	0.0	0.0	0.03711526689351283	0.0014118140155630436
34-35	0.0	0.0	0.0	0.080859441781974	0.0014779927975425613
36-37	0.0	0.0	0.0	0.1420306959250415	0.0014779927975425613
38-39	0.0	0.0	0.0	0.2053968796704297	0.00151108218853232
40-41	0.0	0.0	0.0	0.27177419799588587	0.0015221119855289063
42-43	0.0	0.0	0.0	0.3354271564631853	0.001588290767508424
44-45	0.0	0.0	0.0	0.405576665361474	0.0016875589404777005
46-47	0.0	0.0	0.0	0.49909831409552907	0.0017757973164503906
48-49	0.0	0.0	0.0	0.6023923629685595	0.0018640356924230811
50-51	0.0	0.0	0.0	0.6988699972976997	0.00198536345938553
52-53	0.0	0.0	0.0	0.7854318441269088	0.0021177210233445654
54-55	0.0	0.0	0.0	0.8762401627998037	0.002172870008327497
56-57	0.0	0.0	0.0	0.9863065070287381	0.002338316963276291
58-59	0.0	0.0	0.0	1.1415619295526866	0.0024265553392489813
60-61	0.0	0.0	0.0	1.3377268691369735	0.0024596447302387397
62-63	0.0	0.0	0.0	1.4968096312187373	0.0026030320911943614
64-65	0.0	0.0	0.0	1.627777440756203	0.0026471512791807067
66-67	0.0	0.0	0.0	1.7713192188697766	0.0026802406701704655
68-69	0.0	0.0	0.0	1.9258025556039642	0.0027023002641636384
70-71	0.0	0.0	0.0	2.1047058628885935	0.002790538640136328
72-73	0.0	0.0	0.0	2.2927418420863965	0.002967015392081709
74-75	0.0	0.0	0.0	2.471424553431094	0.003132462347030503
76-77	0.0	0.0	0.0	2.636529584672994	0.0031986411290100204
78-79	0.0	0.0	0.0	2.829705449271206	0.003297909301979297
80-81	0.0	0.0	0.0	3.0408267935828643	0.003353058286962228
82-83	0.0	0.0	0.0	3.256238728926194	0.003485415850921264
84-85	0.0	0.0	0.0	3.552267450517573	0.0035074754449144364
86-87	0.0	0.0	0.0	3.855101556855846	0.003606743617883713
88	0.0	0.0	0.0	4.038957242991943	0.0037060117908529894
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	3675	0.0	26.086586	2
GTACCCG	600	0.0	25.860798	1
GTACTAG	1665	0.0	25.133617	1
TCTAACG	695	0.0	23.666151	2
ACCTGGG	4075	0.0	22.142057	3
GTGATCG	1005	0.0	21.975939	8
TAGGCAT	2140	0.0	21.740074	5
CATGGGG	7580	0.0	21.203196	4
GGGCTAT	2305	0.0	20.998888	6
TGATCGC	1090	0.0	20.26222	9
GCATCGT	1685	0.0	20.079285	8
TTTAGGG	2310	0.0	19.93694	2
GTACCTG	5120	0.0	19.469067	1
CTAACGC	845	0.0	19.46506	3
CTAGGCA	2490	0.0	19.439373	4
GGGCACC	2045	0.0	19.307293	94
CTATTGA	2465	0.0	19.25396	9
TAACGCC	835	0.0	19.135368	4
TATACTC	7975	0.0	19.09232	4
GGCTATT	2515	0.0	19.05718	7
>>END_MODULE