FastQCFastQC Report
Mon 16 Jan 2017
ERR1146533_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146533_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4533175
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1528353.3714780479465274No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1211442.672387454708896No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT489631.0801039006877078No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG302300.6668615264136064No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG265320.5852851478268543No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG217370.47950939462959186No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT206800.45619240377880843No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA179400.39574911623751563No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA137810.304003264819911No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT116170.25626630341868556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113330.2500013787246246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102990.22719175853568416No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC91410.20164674869159036No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG85210.18796980041582334No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG72000.1588290767508424No Hit
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA67760.14947580889773723No Hit
ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT62700.13831365433719192No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC60850.13423262944845502No Hit
GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC59580.1314310610113221No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51020.11254804855316639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG39750.024.273352
GTACCCG6450.022.6221641
GAGTACT119850.021.8998612-13
ACCTGGG42100.021.8921783
GTACTAG17000.021.5962261
TCTAACG6100.020.8327642
TATACTC85000.020.4135074
GGGCACC20700.019.97876294
GTACTTT131550.019.86278714-15
CTAGTAC27400.019.3929043
ATACTCT90150.019.3516655
TAGTACT28050.019.2785854
GGACCGT2201.4899688E-619.227556
CTGTGCG23500.018.9981779
TAGGCAT21650.018.896075
AGTACTT127650.018.86814712-13
CTAACGC7250.018.8094023
CATGGGG76700.018.698844
CGGGCGT10500.018.3526466
GTACGCA13450.018.1800923
Produced by FastQC (version 0.11.5)