FastQCFastQC Report
Mon 16 Jan 2017
ERR1146532_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146532_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4002482
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51650.035.2232671
CAACGCA50650.033.770485
ATCAACG51050.033.322193
GGTATCA31200.032.695961
AACGCAG58150.030.060986
TCAACGC57100.029.9565244
TATCAAC64100.027.4172232
ACGCAGA63700.026.7772677
CGCAGAG66250.025.3219788
TACATGG99650.023.5777632
GTACATG107300.023.2639661
ACATGGG103000.022.1271383
GCATCGT10150.021.2964448
GTACTAG12750.020.2787651
CATGGGG75650.019.567164
AGAGTAC64850.018.6949810-11
GCAGAGT86650.018.3842749
CTAACGC4400.018.1558653
CTAGTAC18450.018.083513
TAACGCC4700.017.9970284
Produced by FastQC (version 0.11.5)