FastQCFastQC Report
Mon 16 Jan 2017
ERR1146532_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146532_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4002482
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94010.2348792574207704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88460.22101286151942717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48590.12139967150383187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63950.055.3294331
GTATCAA117100.043.033981
ATCAACG138600.035.4134863
CAACGCA141000.034.810275
TCAACGC145500.033.83064
AACGCAG151000.032.8678976
TATCAAC151250.032.8181652
ACGCAGA165850.029.5834127
CGCAGAG169400.028.9357078
GTACTAG13550.025.34911
GCAGAGT194300.024.8647179
GAGTACT133750.023.29608512-13
GCATCGT11550.022.7860628
TACATGG100550.022.3667582
AGAGTAC173850.022.22083510-11
CAGAGTA187050.021.98434610-11
GTACATG109200.021.5009121
TAACGCC3750.021.3129564
ACATGGG105300.021.0739083
CGACGAG6150.021.01484724-25
Produced by FastQC (version 0.11.5)