##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146532_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4002482 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.407260544831935 34.0 31.0 34.0 31.0 34.0 2 32.53968037832525 34.0 31.0 34.0 31.0 34.0 3 32.663163507043876 34.0 31.0 34.0 31.0 34.0 4 36.143095709112494 37.0 35.0 37.0 35.0 37.0 5 36.167198003638745 37.0 37.0 37.0 35.0 37.0 6 36.249764271269676 37.0 37.0 37.0 35.0 37.0 7 36.232148202040634 37.0 37.0 37.0 35.0 37.0 8 36.23713885534026 37.0 37.0 37.0 35.0 37.0 9 38.04138007366429 39.0 38.0 39.0 35.0 39.0 10-11 38.00223886078688 39.0 38.0 39.0 35.0 39.0 12-13 37.98728613895078 39.0 38.0 39.0 35.0 39.0 14-15 39.44177700237003 41.0 39.0 41.0 36.5 41.0 16-17 39.43259207661646 41.0 39.0 41.0 36.0 41.0 18-19 39.47538190053072 41.0 39.0 41.0 36.5 41.0 20-21 39.50858642212507 41.0 39.0 41.0 37.0 41.0 22-23 39.472666835228736 41.0 39.0 41.0 36.5 41.0 24-25 39.46771128514757 41.0 39.0 41.0 36.0 41.0 26-27 39.400953708224044 41.0 39.0 41.0 36.0 41.0 28-29 39.31568599184206 41.0 39.0 41.0 36.0 41.0 30-31 39.23221753402014 41.0 39.0 41.0 36.0 41.0 32-33 39.13530779151536 41.0 39.0 41.0 35.0 41.0 34-35 39.06999944034726 40.5 39.0 41.0 35.0 41.0 36-37 39.00015952601411 40.0 39.0 41.0 35.0 41.0 38-39 38.90946854976487 40.0 39.0 41.0 35.0 41.0 40-41 38.83322385959512 40.0 39.0 41.0 35.0 41.0 42-43 38.75695143663357 40.0 38.0 41.0 35.0 41.0 44-45 38.63998476445366 40.0 38.0 41.0 35.0 41.0 46-47 38.449460984459144 40.0 38.0 41.0 34.5 41.0 48-49 38.28794845298492 40.0 38.0 41.0 34.0 41.0 50-51 38.170847614055475 40.0 38.0 41.0 34.0 41.0 52-53 38.063393414386375 40.0 38.0 41.0 34.0 41.0 54-55 37.95055268206078 40.0 37.5 41.0 34.0 41.0 56-57 37.78820816683248 40.0 37.0 41.0 33.0 41.0 58-59 37.61885200233255 40.0 37.0 41.0 33.0 41.0 60-61 37.460727118822774 40.0 36.0 41.0 33.0 41.0 62-63 37.2311839253743 39.0 36.0 41.0 33.0 41.0 64-65 36.95757932203068 39.0 35.0 41.0 32.0 41.0 66-67 36.71543644668483 39.0 35.0 41.0 32.0 41.0 68-69 36.40906667412871 37.5 35.0 40.0 32.0 41.0 70-71 36.12564078988987 37.0 35.0 40.0 32.0 41.0 72-73 35.79066776565141 37.0 35.0 39.0 31.5 41.0 74-75 35.443113048353496 36.0 35.0 39.0 31.0 41.0 76-77 35.10736812807653 36.0 35.0 38.5 31.0 40.0 78-79 34.78581327786109 35.0 35.0 37.0 31.0 39.0 80-81 34.48951538070627 35.0 35.0 37.0 31.0 39.0 82-83 34.203929711613945 35.0 34.5 36.5 31.0 39.0 84-85 33.97042097878267 35.0 34.0 36.0 31.0 37.0 86-87 33.75872858391368 35.0 34.0 36.0 30.0 37.0 88-89 33.58111379389089 35.0 34.0 35.5 30.0 37.0 90-91 33.474489329371124 35.0 34.0 35.0 30.0 36.0 92-93 33.37728564425774 35.0 34.0 35.0 30.0 36.0 94-95 33.28963253301326 35.0 34.0 35.0 30.0 36.0 96-97 33.23498581630099 35.0 34.0 35.0 30.0 36.0 98-99 33.17778443475824 35.0 34.0 35.0 30.0 35.5 100 32.639103686162734 35.0 33.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3160568596335409 1101 2 0.32468106190927415 1101 3 0.3126770498207492 1101 4 0.22563045393241765 1101 5 0.18166570304357776 1101 6 0.18346095270112528 1101 7 0.19794497043144332 1101 8 0.20971870229239897 1101 9 0.2858452103347844 1101 10-11 0.2665518927977999 1101 12-13 0.26094160960173696 1101 14-15 0.40582309451689014 1101 16-17 0.38130822103025963 1101 18-19 0.3734135102769187 1101 20-21 0.3755341671957311 1101 22-23 0.39438019530947344 1101 24-25 0.35701837513013857 1101 26-27 0.36604006772831355 1101 28-29 0.4105978155535155 1101 30-31 0.4117127385786148 1101 32-33 0.3945257531771915 1101 34-35 0.3719709617004483 1101 36-37 0.39515419166625065 1101 38-39 0.41634543458901874 1101 40-41 0.4112435220954467 1101 42-43 0.3520876289282029 1101 44-45 0.35321624185747424 1101 46-47 0.33812985580995303 1101 48-49 0.26415477035127566 1101 50-51 0.24597195586980547 1101 52-53 0.26280076596286506 1101 54-55 0.247966537546624 1101 56-57 0.273226491759047 1101 58-59 0.28831130789757253 1101 60-61 0.2875864081280426 1101 62-63 0.2817220643758702 1101 64-65 0.2748601382360363 1101 66-67 0.2789738449337378 1101 68-69 0.24714209795732955 1101 70-71 0.2007816579604622 1101 72-73 0.19594615848037478 1101 74-75 0.21870549317188193 1101 76-77 0.237920323637546 1101 78-79 0.237156301291769 1101 80-81 0.2273216001207814 1101 82-83 0.21394598633077067 1101 84-85 0.21844750446394556 1101 86-87 0.22608126917752003 1101 88-89 0.18075378595525393 1101 90-91 0.2492974426238348 1101 92-93 0.24377624977424261 1101 94-95 0.24835445665262057 1101 96-97 0.20714572263580067 1101 98-99 0.20226495132264688 1101 100 0.27862592331889857 1102 1 0.2857154030863924 1102 2 0.30626422874629355 1102 3 0.32092425714039763 1102 4 0.1764579696812092 1102 5 0.15032563616409078 1102 6 0.15550483387023917 1102 7 0.22332099295343255 1102 8 0.19208856923174977 1102 9 0.2318675723595689 1102 10-11 0.24933773944865578 1102 12-13 0.25597733068684647 1102 14-15 0.33686603903158385 1102 16-17 0.34465462939513714 1102 18-19 0.3161129000741596 1102 20-21 0.32626778648069177 1102 22-23 0.3234608524944136 1102 24-25 0.31777003828766226 1102 26-27 0.33780885763584223 1102 28-29 0.3804560355684856 1102 30-31 0.37707579446274764 1102 32-33 0.33941647466991753 1102 34-35 0.322507967215671 1102 36-37 0.30306687555061984 1102 38-39 0.36498394636454634 1102 40-41 0.36133477755714694 1102 42-43 0.3206905241035969 1102 44-45 0.2500852839605727 1102 46-47 0.25517116615864666 1102 48-49 0.2630755823265858 1102 50-51 0.26127142643376544 1102 52-53 0.27607382307526507 1102 54-55 0.2722517684361918 1102 56-57 0.2462396679619161 1102 58-59 0.30943963991379064 1102 60-61 0.2776033675970879 1102 62-63 0.28565600242926337 1102 64-65 0.30157754131292336 1102 66-67 0.28359570365947917 1102 68-69 0.2886506355698728 1102 70-71 0.22185843005451034 1102 72-73 0.21988581888005854 1102 74-75 0.2725696853169808 1102 76-77 0.21492319821696526 1102 78-79 0.17273585389375512 1102 80-81 0.1810186723117866 1102 82-83 0.2525885903469671 1102 84-85 0.19369492645908792 1102 86-87 0.14652590192437742 1102 88-89 0.1549862860608755 1102 90-91 0.10785627244252538 1102 92-93 0.10752089465677273 1102 94-95 0.13001475685317132 1102 96-97 0.14269641498263752 1102 98-99 0.13086809292155976 1102 100 0.16469218163424415 1103 1 0.2697840671632932 1103 2 0.26592190273761673 1103 3 0.29860592887336423 1103 4 0.20305228730190095 1103 5 0.1523749934013665 1103 6 0.1439359231063193 1103 7 0.15507831227711932 1103 8 0.16166247226152564 1103 9 0.2189647834127868 1103 10-11 0.20042706983085168 1103 12-13 0.24102097192742633 1103 14-15 0.2927949597448105 1103 16-17 0.2743718660816157 1103 18-19 0.26894230145091313 1103 20-21 0.2999768783000647 1103 22-23 0.3196112693798838 1103 24-25 0.31663300699040775 1103 26-27 0.31950995034669205 1103 28-29 0.3685816350803677 1103 30-31 0.37241087865565703 1103 32-33 0.32881253837015834 1103 34-35 0.3151175671583175 1103 36-37 0.3642769650395863 1103 38-39 0.3987478418824111 1103 40-41 0.36983670809549096 1103 42-43 0.34485127334426835 1103 44-45 0.3386499135691068 1103 46-47 0.35533844180537244 1103 48-49 0.33603107735203963 1103 50-51 0.33502098324656515 1103 52-53 0.33129149811227876 1103 54-55 0.293485453894597 1103 56-57 0.3171877581466731 1103 58-59 0.34488889495364106 1103 60-61 0.26848600233517317 1103 62-63 0.2679647981409943 1103 64-65 0.2641583958894813 1103 66-67 0.21575144940077706 1103 68-69 0.19144256409519045 1103 70-71 0.2373440477674933 1103 72-73 0.229068799770495 1103 74-75 0.22240656330423292 1103 76-77 0.25179365136595777 1103 78-79 0.2285444163016237 1103 80-81 0.25484136844738003 1103 82-83 0.2756183064429649 1103 84-85 0.2737698471985297 1103 86-87 0.26852823849951335 1103 88-89 0.2972585471293243 1103 90-91 0.20303128223893196 1103 92-93 0.16811248100309228 1103 94-95 0.23300412861222242 1103 96-97 0.19383675046451287 1103 98-99 0.21687969383403072 1103 100 0.2618530653306266 1104 1 0.16855008241834923 1104 2 0.22018587469155904 1104 3 0.20501234123382517 1104 4 0.14654871404641057 1104 5 0.15117567572584534 1104 6 0.13616492644811018 1104 7 0.1652941713558036 1104 8 0.13193937176090031 1104 9 0.19903057196771812 1104 10-11 0.1899129006845115 1104 12-13 0.22276186138019938 1104 14-15 0.2919999188037963 1104 16-17 0.25239115317874194 1104 18-19 0.2650075050232914 1104 20-21 0.2957835012232053 1104 22-23 0.3427644042087934 1104 24-25 0.2721022447183117 1104 26-27 0.28948847512517517 1104 28-29 0.3434863491294209 1104 30-31 0.34236671014551234 1104 32-33 0.32859843915277054 1104 34-35 0.359717896320916 1104 36-37 0.3509120080805417 1104 38-39 0.3316794436639512 1104 40-41 0.32987204885170485 1104 42-43 0.3155537851486585 1104 44-45 0.3298363833519886 1104 46-47 0.3000094563595965 1104 48-49 0.29720237560469087 1104 50-51 0.2979041377662668 1104 52-53 0.28804293897447053 1104 54-55 0.2682214229768931 1104 56-57 0.2527751858837348 1104 58-59 0.24133382642378365 1104 60-61 0.239774078570143 1104 62-63 0.21790864033329171 1104 64-65 0.2592511176927843 1104 66-67 0.24404390291898181 1104 68-69 0.21812017353890667 1104 70-71 0.15626458039643154 1104 72-73 0.16461439685871682 1104 74-75 0.15105417648749153 1104 76-77 0.18065148457196756 1104 78-79 0.17483780703324214 1104 80-81 0.1952187287433631 1104 82-83 0.17360311655121308 1104 84-85 0.1973295733480782 1104 86-87 0.1930779674472518 1104 88-89 0.23763632767138176 1104 90-91 0.21551294657893294 1104 92-93 0.17356915014036645 1104 94-95 0.194642611205758 1104 96-97 0.1676005610439546 1104 98-99 0.14932406876904736 1104 100 0.18253698286204667 1105 1 0.19451409417919763 1105 2 0.20581615003529663 1105 3 0.19408138718416268 1105 4 0.13856136095107985 1105 5 0.08526258203973214 1105 6 0.11767339786487696 1105 7 0.09407828670796903 1105 8 0.09532904603774028 1105 9 0.08829416938749546 1105 10-11 0.1292462509643144 1105 12-13 0.13496446605186208 1105 14-15 0.17125015272553412 1105 16-17 0.18058003908182485 1105 18-19 0.14548233286535606 1105 20-21 0.2072374806768309 1105 22-23 0.22228222583756718 1105 24-25 0.19357029881627597 1105 26-27 0.21167944725913657 1105 28-29 0.24928434000495514 1105 30-31 0.22291177503231552 1105 32-33 0.20822199461794355 1105 34-35 0.1694959763106425 1105 36-37 0.231082692269851 1105 38-39 0.19413871162507945 1105 40-41 0.2054697597474373 1105 42-43 0.179382100512683 1105 44-45 0.19719083440151763 1105 46-47 0.26308239818116874 1105 48-49 0.25277650670496854 1105 50-51 0.21034359954654747 1105 52-53 0.19251096523181133 1105 54-55 0.17078844927175396 1105 56-57 0.23997883457867886 1105 58-59 0.18227755992368344 1105 60-61 0.2110738784808106 1105 62-63 0.2188357027483221 1105 64-65 0.2574552009434967 1105 66-67 0.1956355693738132 1105 68-69 0.23875364450174885 1105 70-71 0.20173183982623044 1105 72-73 0.1196883046287951 1105 74-75 0.11640006291470684 1105 76-77 0.10923142528354646 1105 78-79 0.16299274250957296 1105 80-81 0.1395165156560907 1105 82-83 0.13709867597218306 1105 84-85 0.13507696147417647 1105 86-87 0.1403885332049839 1105 88-89 0.16389912679161966 1105 90-91 0.1343159118471604 1105 92-93 0.15877836635426945 1105 94-95 0.16418886052941417 1105 96-97 0.10266741182185513 1105 98-99 0.06132227183853445 1105 100 0.08922865766906796 1106 1 0.17844371092336075 1106 2 0.14970281260477236 1106 3 0.15468071030636565 1106 4 0.12011770202351357 1106 5 0.04956911414497256 1106 6 0.08212665024542787 1106 7 0.10141040681152447 1106 8 0.09823132883001051 1106 9 0.16043939591236267 1106 10-11 0.15020910940801002 1106 12-13 0.14249724250299067 1106 14-15 0.2246966411952087 1106 16-17 0.23675633880178992 1106 18-19 0.19893262650501242 1106 20-21 0.18223946069260677 1106 22-23 0.23352982161170388 1106 24-25 0.19024537667846175 1106 26-27 0.17329550295266216 1106 28-29 0.1899746155390858 1106 30-31 0.2077169348428214 1106 32-33 0.2611241225486438 1106 34-35 0.20735666549772702 1106 36-37 0.2427219550756874 1106 38-39 0.3326249088134148 1106 40-41 0.28498816681478445 1106 42-43 0.26797451061443667 1106 44-45 0.3265379866963727 1106 46-47 0.32628215677411276 1106 48-49 0.28763416458744473 1106 50-51 0.31387621317179537 1106 52-53 0.3152743327718497 1106 54-55 0.3006399635745538 1106 56-57 0.2967808192845496 1106 58-59 0.3168406268552957 1106 60-61 0.2957092657652609 1106 62-63 0.27615373044908154 1106 64-65 0.31283316715094145 1106 66-67 0.2965356248142541 1106 68-69 0.2668200473727964 1106 70-71 0.28632240574793144 1106 72-73 0.2766525400130746 1106 74-75 0.2760473856015224 1106 76-77 0.20189311071722216 1106 78-79 0.20187764186947277 1106 80-81 0.20610968944661323 1106 82-83 0.2304211778448959 1106 84-85 0.1870903167359046 1106 86-87 0.2116806052218152 1106 88-89 0.1980404393009465 1106 90-91 0.21383206236021834 1106 92-93 0.17573548695558827 1106 94-95 0.18830138206070046 1106 96-97 0.15345284757798083 1106 98-99 0.1375095623053113 1106 100 0.21464759139849576 1107 1 0.07181761452535085 1107 2 0.10462125485071283 1107 3 0.08822684658245805 1107 4 0.09721916678471132 1107 5 0.05123744911860939 1107 6 0.0565961773501229 1107 7 0.06949531361202332 1107 8 0.07880218886661794 1107 9 0.06684734675314274 1107 10-11 0.07143897925990217 1107 12-13 0.11193216749861534 1107 14-15 0.15620589170255528 1107 16-17 0.10187314605089881 1107 18-19 0.13838193175792668 1107 20-21 0.09845950943024917 1107 22-23 0.10485094992707644 1107 24-25 0.07083014487339767 1107 26-27 0.13462490056000576 1107 28-29 0.18608059721761094 1107 30-31 0.13180067926064964 1107 32-33 0.1077104719110551 1107 34-35 0.0993784368197197 1107 36-37 0.13733033023430607 1107 38-39 0.1418473142376584 1107 40-41 0.08861402957142417 1107 42-43 0.12235669493284718 1107 44-45 0.11380634879749607 1107 46-47 0.11842331648882265 1107 48-49 0.06295832520054745 1107 50-51 0.11284341691121824 1107 52-53 0.11266659928699596 1107 54-55 0.07227038390614382 1107 56-57 0.10376922502710784 1107 58-59 0.10515630099971673 1107 60-61 0.05317212535430116 1107 62-63 0.06646420467275505 1107 64-65 0.10209557330097141 1107 66-67 0.07575863394273341 1107 68-69 0.11467651372277743 1107 70-71 0.06064514816009847 1107 72-73 0.05627051899721636 1107 74-75 0.09056330552861169 1107 76-77 0.08870955707664052 1107 78-79 0.05762670105970358 1107 80-81 0.11443703333950594 1107 82-83 0.10557137587444743 1107 84-85 0.06386428595012461 1107 86-87 0.11179999361453952 1107 88-89 0.08033450811404208 1107 90-91 0.13532904893524744 1107 92-93 0.1007695055212281 1107 94-95 0.09734394466534013 1107 96-97 0.08922700529576844 1107 98-99 0.08196964071069601 1107 100 0.11120043461583151 1108 1 0.11855252284725992 1108 2 0.1030133824897348 1108 3 0.10898235933329659 1108 4 0.11260928862636632 1108 5 0.11220807118817078 1108 6 0.06617510460390008 1108 7 0.08299305735598494 1108 8 0.08353324050791855 1108 9 0.11378291992899392 1108 10-11 0.059985921004106046 1108 12-13 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76-77 -0.2705287798699132 2214 78-79 -0.27032135921825784 2214 80-81 -0.2722897302058911 2214 82-83 -0.2637957161774054 2214 84-85 -0.2625051847362485 2214 86-87 -0.27879934181897426 2214 88-89 -0.2817130966698329 2214 90-91 -0.23204243666015856 2214 92-93 -0.23218051292086272 2214 94-95 -0.24530818398930876 2214 96-97 -0.29811985906020055 2214 98-99 -0.3002356474487371 2214 100 -0.24038023639724315 2215 1 -0.31577012202374277 2215 2 -0.31894069257849367 2215 3 -0.2989718459263315 2215 4 -0.23221320707413895 2215 5 -0.24479500685951905 2215 6 -0.2133898087784516 2215 7 -0.22541650865492358 2215 8 -0.22360768928199093 2215 9 -0.22465603189623806 2215 10-11 -0.2930404580414674 2215 12-13 -0.27744811580054574 2215 14-15 -0.4214068737725114 2215 16-17 -0.3854404790673769 2215 18-19 -0.34007744855357913 2215 20-21 -0.33391267459521856 2215 22-23 -0.33850259383351755 2215 24-25 -0.2841053726305489 2215 26-27 -0.37234587217040627 2215 28-29 -0.33317624536495316 2215 30-31 -0.37688262328823185 2215 32-33 -0.3968439922271685 2215 34-35 -0.34039587532556936 2215 36-37 -0.3274249550261672 2215 38-39 -0.32050497287587376 2215 40-41 -0.36430831394137897 2215 42-43 -0.37496656130626604 2215 44-45 -0.36926446171342064 2215 46-47 -0.36242762468306466 2215 48-49 -0.3532916040601677 2215 50-51 -0.313285132548188 2215 52-53 -0.412700949706462 2215 54-55 -0.42841673477384035 2215 56-57 -0.345446115410887 2215 58-59 -0.33933861344056027 2215 60-61 -0.3741114137502919 2215 62-63 -0.3751285478867743 2215 64-65 -0.3373950765079172 2215 66-67 -0.34799283307107487 2215 68-69 -0.3599311454776384 2215 70-71 -0.34294705452818874 2215 72-73 -0.31057330734501676 2215 74-75 -0.3320757476350735 2215 76-77 -0.29176614854003446 2215 78-79 -0.31183258369867417 2215 80-81 -0.2812209319394512 2215 82-83 -0.2857737186623268 2215 84-85 -0.27158343952397246 2215 86-87 -0.24362712887699445 2215 88-89 -0.19495707878544977 2215 90-91 -0.18384535703545168 2215 92-93 -0.22446174803887686 2215 94-95 -0.2293072292894891 2215 96-97 -0.25065411215400957 2215 98-99 -0.2532540549846729 2215 100 -0.24164567354220168 2216 1 -0.45303268797092144 2216 2 -0.44989794674615524 2216 3 -0.4266714657149322 2216 4 -0.3676284157062284 2216 5 -0.3738582466057352 2216 6 -0.40407300086399545 2216 7 -0.3481304204835567 2216 8 -0.41086994711620406 2216 9 -0.500396889524275 2216 10-11 -0.5024514164752603 2216 12-13 -0.514913640160394 2216 14-15 -0.6144651766686877 2216 16-17 -0.6160619341176599 2216 18-19 -0.6729737016358115 2216 20-21 -0.6649587093133817 2216 22-23 -0.6409588263866226 2216 24-25 -0.6621564612816968 2216 26-27 -0.6968150155371333 2216 28-29 -0.6844457148032319 2216 30-31 -0.6829825117513337 2216 32-33 -0.7063568920367516 2216 34-35 -0.7721530347705041 2216 36-37 -0.7182015493567135 2216 38-39 -0.6719648121113266 2216 40-41 -0.6488268698247097 2216 42-43 -0.6962269282071887 2216 44-45 -0.7247678434733089 2216 46-47 -0.7471049027602987 2216 48-49 -0.7405301812120939 2216 50-51 -0.6728756328092018 2216 52-53 -0.6908624748480321 2216 54-55 -0.6669668167759042 2216 56-57 -0.6714340356219566 2216 58-59 -0.6418194950309939 2216 60-61 -0.6469654729625631 2216 62-63 -0.6066349473930899 2216 64-65 -0.5438063351317766 2216 66-67 -0.5110992544093946 2216 68-69 -0.5214677312514482 2216 70-71 -0.5391179586284665 2216 72-73 -0.5542900036345486 2216 74-75 -0.5510663783599199 2216 76-77 -0.5338775762738948 2216 78-79 -0.5681418569306089 2216 80-81 -0.4868997620257076 2216 82-83 -0.5024147679366351 2216 84-85 -0.5143546691676093 2216 86-87 -0.509331206620061 2216 88-89 -0.46414899530972775 2216 90-91 -0.43684615724884424 2216 92-93 -0.5172923114366341 2216 94-95 -0.44519252147301813 2216 96-97 -0.4425233190800384 2216 98-99 -0.439988119161967 2216 100 -0.629394397734437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 20.0 8 65.0 9 117.0 10 209.0 11 189.0 12 155.0 13 227.0 14 333.0 15 655.0 16 1287.0 17 2352.0 18 4044.0 19 6533.0 20 9587.0 21 14271.0 22 20297.0 23 25086.0 24 24505.0 25 21138.0 26 19217.0 27 20514.0 28 24550.0 29 30902.0 30 38717.0 31 49781.0 32 64511.0 33 86507.0 34 125148.0 35 194037.0 36 342200.0 37 853410.0 38 1447277.0 39 572264.0 40 2377.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.24510116247837 19.881465934573896 13.712422465003844 24.161010437943894 2 14.997302153326702 21.2634073478442 40.43872137569678 23.300569123132323 3 17.7127006918483 26.278992431417926 31.065265497504413 24.94304137922936 4 11.847573580593242 18.314960566968196 40.07573300766874 29.761732844769824 5 12.400589955608366 38.401076336566625 36.130831978873175 13.06750172895184 6 28.884447618129112 39.51200767147766 18.635839172807632 12.967705537585594 7 25.403499830105336 33.42814954728068 23.54520197477564 17.62314864783834 8 24.682915421709684 35.78842722801183 21.27745265998764 18.251204690290848 9 25.753844739339236 15.188200721452338 21.03789598554097 38.020058553667454 10-11 23.654847167332672 26.566390554660835 29.924144568295375 19.854617709711125 12-13 24.842147947198765 24.47004133934893 29.246664944402 21.441145769050305 14-15 21.984167068396708 25.62620854742453 27.36494281483695 25.024681569341812 16-17 21.18819022796355 28.41463122132717 28.013492627824434 22.38368592288485 18-19 21.037059004887467 27.562747315290864 30.212165851089402 21.188027828732274 20-21 22.080324158859426 26.851038930343723 29.98498181878145 21.083655092015405 22-23 21.75151032115249 26.862604498233587 29.629552825012617 21.756332355601305 24-25 21.52932855163371 26.903806188260184 30.00240850552232 21.56445675458378 26-27 21.569753467973126 27.130290654648793 29.64441813854503 21.655537738833054 28-29 21.388819279251685 27.279406088076914 29.83367308110798 21.49810155156342 30-31 21.753994058428574 26.935998250083415 29.776939352423433 21.533068339064577 32-33 21.641728396048787 27.181919620549188 29.506015866416686 21.670336116985343 34-35 21.753423391179478 27.220130783130987 29.675678072869527 21.35076775282001 36-37 21.87307890984204 27.027200231858135 29.50269803663883 21.597022821660996 38-39 21.725357355940808 27.153892164103404 29.614433852048695 21.506316627907093 40-41 21.896670854546002 26.922788155726153 29.583363679530667 21.597177310197186 42-43 21.8821694359493 26.914124234640752 29.407234393029967 21.796471936379973 44-45 21.97403994596319 27.318294737457517 29.02946864888242 21.678196667696866 46-47 22.019973423900776 27.217915643808315 28.658876390907263 22.10323454138365 48-49 22.15372530481947 27.214999009357015 28.63957609974488 21.99169958607863 50-51 22.09528597071816 27.48535177172262 28.341653036461572 22.077709221097646 52-53 22.053881307516665 27.622352637537173 28.267973407135223 22.05579264781094 54-55 22.095609917950878 27.401113251621922 28.30140618693268 22.201870643494516 56-57 22.20096576016198 27.49350833978148 28.130431838754482 22.175094061302058 58-59 22.07095269445593 27.51930411492148 28.39500697708991 22.014736213532682 60-61 22.151314828702304 27.58599926269303 28.235508988536512 22.027176920068154 62-63 22.051937100829182 27.471047330532954 28.221689907712488 22.255325660925372 64-65 21.87900784272279 27.43556282136137 28.300988254530075 22.38444108138577 66-67 22.00609871960369 27.472609293295342 28.12811733607708 22.39317465102389 68-69 21.919570711992062 27.40262715785834 28.29643998972603 22.381362140423573 70-71 21.99458850974644 27.12492312443292 28.236928280327138 22.6435600854935 72-73 22.040114684773034 27.164812512883294 28.32795097788133 22.46712182446234 74-75 22.017017292756577 27.379479582009576 28.039393449907625 22.564109675326225 76-77 22.010090604564283 27.219971731515862 28.064215981812623 22.70572168210723 78-79 22.008421097341063 27.317772437989507 27.991210378149873 22.68259608651956 80-81 21.925711540272914 27.415696622018505 28.007205034679757 22.65138680302882 82-83 22.072399684595236 27.17214294351796 28.30602856831621 22.44942880357059 84-85 22.225724220276593 27.316871527306713 27.926106758389867 22.531297494026823 86-87 21.847321467944326 27.44694759988272 28.075732517439494 22.62999841473346 88-89 22.06129405897749 27.20273650610272 28.02058026081855 22.71538917410123 90-91 21.982802666646013 27.265438611701093 27.995285923905698 22.7564727977472 92-93 22.102447931058776 27.19265195945916 28.035104217832835 22.66979589164923 94-95 21.749472389750256 27.269673485020917 28.14872825130213 22.8321258739267 96-97 21.80018790917748 27.271503027321536 28.098255937496525 22.83005312600446 98-99 21.676825832906303 27.294582008419322 28.01091628375642 23.017675874917956 100 21.930220809533353 27.148669291389734 27.833948872366804 23.087161026710113 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 338.0 1 211.5 2 213.5 3 331.5 4 220.5 5 174.5 6 367.5 7 949.0 8 1837.0 9 2324.0 10 1809.0 11 1266.0 12 1397.5 13 1665.5 14 1976.0 15 2377.5 16 2853.5 17 3430.5 18 4058.0 19 4794.5 20 5809.5 21 7217.0 22 8990.5 23 11460.5 24 14581.0 25 18353.5 26 24234.5 27 31582.5 28 38678.5 29 46833.5 30 55888.0 31 63487.5 32 71766.0 33 80654.5 34 88641.0 35 97710.5 36 108290.0 37 119273.0 38 125139.5 39 127512.0 40 131327.0 41 133728.0 42 136225.0 43 141405.5 44 148067.5 45 156068.0 46 159948.5 47 162148.5 48 164810.0 49 162172.5 50 154809.0 51 147622.0 52 139968.5 53 130700.0 54 121289.0 55 108352.5 56 96238.0 57 85267.0 58 74929.0 59 67007.5 60 54795.5 61 41302.5 62 31176.5 63 23011.0 64 16465.5 65 10839.0 66 7257.5 67 5069.0 68 3512.5 69 2398.5 70 1708.5 71 1277.0 72 912.5 73 688.0 74 520.0 75 396.0 76 270.5 77 138.5 78 65.5 79 25.5 80 15.0 81 7.5 82 4.5 83 4.5 84 3.5 85 2.5 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006421015759721093 2 0.07485355337013383 3 3.747674567930599E-4 4 0.0 5 0.0375516991706646 6 0.0030980776428226283 7 4.9968994239074655E-5 8 2.4984497119537328E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 3.7476745679305986E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.496589366298212E-4 24-25 0.0 26-27 0.0 28-29 7.495349135861197E-5 30-31 1.374147341574553E-4 32-33 0.0016489768098894636 34-35 0.0013616550930147843 36-37 8.619651506240379E-4 38-39 7.495349135861198E-4 40-41 0.0014615930814929337 42-43 7.870116592654259E-4 44-45 6.246124279884332E-4 46-47 3.372907111137539E-4 48-49 7.745194107056571E-4 50-51 8.869496477435751E-4 52-53 0.001374147341574553 54-55 0.001561531069971083 56-57 9.743953876619558E-4 58-59 0.0024234962205951205 60-61 0.00196128302388368 62-63 0.001111810121819411 64-65 0.004322318001679958 66-67 0.004159918770402966 68-69 0.0033479226140180015 70-71 0.001811376041166456 72-73 0.005171790903744226 74-75 0.0035353063424145314 76-77 0.003622752082332912 78-79 8.619651506240379E-4 80-81 7.495349135861197E-5 82-83 1.4990698271722395E-4 84-85 1.2492248559768662E-4 86-87 1.2492248559768664E-5 88-89 1.4990698271722395E-4 90-91 2.4984497119537328E-5 92-93 0.0 94-95 2.4984497119537328E-5 96-97 4.122442024723659E-4 98-99 5.246744395102839E-4 100 6.995659193470452E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4002482.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.387119492175586 #Duplication Level Percentage of deduplicated Percentage of total 1 70.44242383672102 41.12930217873107 2 16.18744879860578 18.902770145553397 3 6.095443594353397 10.676861805039843 4 2.894608059137659 6.760313065275398 5 1.537494257624892 4.488493046923918 6 0.8570632978723958 3.0024874311140195 7 0.5525734529097569 2.2584220556272223 8 0.36396432726364575 1.7000662933465427 9 0.2462929465186334 1.2942302128617114 >10 0.7987133359425173 6.957832505937188 >50 0.014759836149773971 0.5715543611154671 >100 0.007980385665967179 0.8372351073210696 >500 8.225808230386034E-4 0.3469231548535448 >1k 2.7419360767818354E-4 0.343200975179713 >5k 1.3709680383909177E-4 0.730307661120026 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9401 0.2348792574207704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8846 0.22101286151942717 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4859 0.12139967150383187 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.4984497119537328E-5 0.0 3 0.0 0.0 0.0 2.4984497119537328E-5 0.0 4 0.0 0.0 0.0 2.4984497119537328E-5 0.0 5 0.0 0.0 0.0 2.4984497119537328E-5 0.0 6 0.0 0.0 0.0 2.4984497119537328E-5 0.0 7 0.0 0.0 0.0 2.4984497119537328E-5 0.0 8 0.0 0.0 0.0 2.4984497119537328E-5 0.0 9 0.0 0.0 0.0 2.4984497119537328E-5 0.0 10-11 0.0 0.0 0.0 2.4984497119537328E-5 0.0 12-13 0.0 0.0 0.0 2.4984497119537328E-5 2.4984497119537328E-5 14-15 0.0 0.0 0.0 2.4984497119537328E-5 6.246124279884332E-5 16-17 0.0 0.0 0.0 2.4984497119537328E-5 9.993798847814931E-5 18-19 0.0 0.0 0.0 2.4984497119537328E-5 9.993798847814931E-5 20-21 0.0 0.0 0.0 8.744573991838065E-5 9.993798847814931E-5 22-23 0.0 0.0 0.0 2.498449711953733E-4 9.993798847814931E-5 24-25 0.0 0.0 0.0 5.121821909505153E-4 9.993798847814931E-5 26-27 0.0 0.0 0.0 8.994418963033438E-4 1.1243023703791797E-4 28-29 0.0 0.0 0.0 0.002311065983557203 1.2492248559768665E-4 30-31 0.0 0.0 0.0 0.007370426650263511 1.374147341574553E-4 32-33 0.0 0.0 0.0 0.01925055503060351 1.4990698271722397E-4 34-35 0.0 0.0 0.0 0.03897581550647823 1.748914798367613E-4 36-37 0.0 0.0 0.0 0.07161806099315375 1.748914798367613E-4 38-39 0.0 0.0 0.0 0.11717729149063007 1.748914798367613E-4 40-41 0.0 0.0 0.0 0.16222433979715586 1.748914798367613E-4 42-43 0.0 0.0 0.0 0.20912024089052744 1.748914798367613E-4 44-45 0.0 0.0 0.0 0.26767140989016314 1.748914798367613E-4 46-47 0.0 0.0 0.0 0.3354668428240277 1.748914798367613E-4 48-49 0.0 0.0 0.0 0.4086839116328318 1.748914798367613E-4 50-51 0.0 0.0 0.0 0.48452435263918736 1.748914798367613E-4 52-53 0.0 0.0 0.0 0.5575165609739157 1.748914798367613E-4 54-55 0.0 0.0 0.0 0.636829847079887 1.748914798367613E-4 56-57 0.0 0.0 0.0 0.7297222073703267 1.8738372839652995E-4 58-59 0.0 0.0 0.0 0.835981273619719 1.9987597695629862E-4 60-61 0.0 0.0 0.0 0.9399417661341138 1.9987597695629862E-4 62-63 0.0 0.0 0.0 1.045338867232882 1.9987597695629862E-4 64-65 0.0 0.0 0.0 1.1511357202855628 1.9987597695629862E-4 66-67 0.0 0.0 0.0 1.2705866010140707 1.9987597695629862E-4 68-69 0.0 0.0 0.0 1.4075890909690538 1.9987597695629862E-4 70-71 0.0 0.0 0.0 1.5528114804763644 1.9987597695629862E-4 72-73 0.0 0.0 0.0 1.6964723389137042 2.2486047407583595E-4 74-75 0.0 0.0 0.0 1.839033879477784 2.498449711953733E-4 76-77 0.0 0.0 0.0 1.991851556109434 2.498449711953733E-4 78-79 0.0 0.0 0.0 2.1591852255675352 2.6233721975514195E-4 80-81 0.0 0.0 0.0 2.3408599963722514 2.748294683149106E-4 82-83 0.0 0.0 0.0 2.5262074882535384 2.748294683149106E-4 84-85 0.0 0.0 0.0 2.709556220365263 2.748294683149106E-4 86-87 0.0 0.0 0.0 2.9002379023815723 2.873217168746793E-4 88 0.0 0.0 0.0 3.0505071603070295 2.9981396543444795E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6395 0.0 55.329433 1 GTATCAA 11710 0.0 43.03398 1 ATCAACG 13860 0.0 35.413486 3 CAACGCA 14100 0.0 34.81027 5 TCAACGC 14550 0.0 33.8306 4 AACGCAG 15100 0.0 32.867897 6 TATCAAC 15125 0.0 32.818165 2 ACGCAGA 16585 0.0 29.583412 7 CGCAGAG 16940 0.0 28.935707 8 GTACTAG 1355 0.0 25.3491 1 GCAGAGT 19430 0.0 24.864717 9 GAGTACT 13375 0.0 23.296085 12-13 GCATCGT 1155 0.0 22.786062 8 TACATGG 10055 0.0 22.366758 2 AGAGTAC 17385 0.0 22.220835 10-11 CAGAGTA 18705 0.0 21.984346 10-11 GTACATG 10920 0.0 21.500912 1 TAACGCC 375 0.0 21.312956 4 ACATGGG 10530 0.0 21.073908 3 CGACGAG 615 0.0 21.014847 24-25 >>END_MODULE