FastQCFastQC Report
Mon 16 Jan 2017
ERR1146531_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146531_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3292317
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39600.032.528761
GTATCAA59150.031.8714471
CAACGCA54050.031.5615355
ATCAACG56700.030.2517413
GTACTAG23350.028.5899621
GCATCGT23550.026.5404268
TATCAAC71700.026.1513632
CTGTGCG30650.025.452179
AACGCAG72200.025.3840796
GGGCACC30550.024.7712394
GTACATG113400.024.708441
ACGCAGA68650.024.6423857
TACATGG110600.024.5591472
CGCAGAG70000.023.9672038
CGTTAAC5700.023.9185731
TAGTACT32050.023.9004864
ACATGGG115300.022.9059123
CTAGTAC33750.022.8355063
GCTATTG25850.022.5428248
CATGGGG92300.022.0970974
Produced by FastQC (version 0.11.5)