##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146531_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3292317
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.460541314824788	31.0	31.0	34.0	28.0	34.0
2	31.49782812529899	33.0	31.0	34.0	28.0	34.0
3	31.733585192434386	33.0	31.0	34.0	29.0	34.0
4	35.25744604787449	37.0	35.0	37.0	32.0	37.0
5	35.233778521327075	37.0	35.0	37.0	32.0	37.0
6	35.204394959537616	37.0	35.0	37.0	32.0	37.0
7	35.21213236756971	37.0	35.0	37.0	32.0	37.0
8	35.124865254469725	37.0	35.0	37.0	32.0	37.0
9	36.7509702133786	39.0	38.0	39.0	33.0	39.0
10-11	36.729327552602015	39.0	37.5	39.0	33.0	39.0
12-13	36.67861949502432	39.0	37.0	39.0	33.0	39.0
14-15	38.09473130928765	40.0	38.0	41.0	33.0	41.0
16-17	38.12520331426166	40.0	38.0	41.0	33.0	41.0
18-19	38.16992546586492	40.0	38.5	41.0	33.0	41.0
20-21	38.27776532454196	40.0	39.0	41.0	33.0	41.0
22-23	38.31371098226568	40.0	39.0	41.0	33.0	41.0
24-25	38.37769206306683	40.5	38.5	41.0	33.0	41.0
26-27	38.415905272791164	40.0	38.0	41.0	33.0	41.0
28-29	38.396093693286524	40.0	38.0	41.0	33.0	41.0
30-31	38.39220706876039	40.0	38.0	41.0	33.0	41.0
32-33	38.40491392536016	40.0	38.0	41.0	33.0	41.0
34-35	38.34038338349558	40.0	38.0	41.0	33.0	41.0
36-37	38.24896159756184	40.0	38.0	41.0	33.0	41.0
38-39	38.19703555277332	40.0	38.0	41.0	33.0	41.0
40-41	38.123143974289235	40.0	38.0	41.0	33.0	41.0
42-43	37.99020720058245	40.0	38.0	41.0	32.0	41.0
44-45	37.814718783154845	40.0	37.0	41.0	32.0	41.0
46-47	37.69098236895171	40.0	37.0	41.0	32.0	41.0
48-49	37.551997574960126	40.0	36.5	41.0	31.5	41.0
50-51	37.39595078481203	40.0	36.0	41.0	31.0	41.0
52-53	37.244733724000454	40.0	35.5	41.0	31.0	41.0
54-55	37.05653556446721	39.0	35.0	41.0	31.0	41.0
56-57	36.895574454100256	39.0	35.0	41.0	31.0	41.0
58-59	36.64156185446298	39.0	35.0	41.0	30.0	41.0
60-61	36.453701147246754	38.0	35.0	40.5	30.5	41.0
62-63	36.203589144058725	37.5	35.0	40.0	30.0	41.0
64-65	35.94716532460271	37.0	35.0	40.0	30.0	41.0
66-67	35.69612904832675	36.5	35.0	40.0	30.0	41.0
68-69	35.41343284987442	36.0	35.0	39.5	30.0	41.0
70-71	35.19926498572282	35.5	34.5	39.0	30.0	41.0
72-73	34.9110952863895	35.0	34.0	39.0	29.5	41.0
74-75	34.60727566634684	35.0	34.0	37.5	29.0	40.0
76-77	34.323239681962576	35.0	34.0	37.0	29.0	39.0
78-79	34.05786016352617	35.0	34.0	37.0	29.0	39.0
80-81	33.8136118119853	35.0	34.0	36.0	29.0	39.0
82-83	33.56256763853541	35.0	34.0	36.0	28.0	37.0
84-85	33.35058774717015	35.0	34.0	35.5	28.0	37.0
86-87	33.1567585988834	35.0	34.0	35.0	28.0	37.0
88-89	33.03393294145126	35.0	34.0	35.0	28.0	36.0
90-91	32.865557751577384	35.0	33.5	35.0	27.5	36.0
92-93	32.760493749538696	35.0	33.0	35.0	27.0	36.0
94-95	32.67442078025901	35.0	33.0	35.0	27.0	36.0
96-97	32.58535630074503	35.0	33.0	35.0	27.0	35.0
98-99	32.513938056390074	35.0	33.0	35.0	27.0	35.0
100	31.573411977036233	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17556476331892767
1101	2	0.16876831853901564
1101	3	0.312694729350774
1101	4	0.331632850469326
1101	5	0.27318662503829927
1101	6	0.24548076548974507
1101	7	0.3032632879528734
1101	8	0.2715900415103363
1101	9	0.36109626753705726
1101	10-11	0.3661864995509774
1101	12-13	0.40432064226658326
1101	14-15	0.46591752578726897
1101	16-17	0.3884645702414531
1101	18-19	0.3310639081144373
1101	20-21	0.36115196636832536
1101	22-23	0.2937468182410825
1101	24-25	0.3049647682528587
1101	26-27	0.3903031315092491
1101	28-29	0.4149210263821459
1101	30-31	0.41982081573745944
1101	32-33	0.37072511124810603
1101	34-35	0.3619781321040847
1101	36-37	0.406084571044552
1101	38-39	0.37447030349600396
1101	40-41	0.40529931805031083
1101	42-43	0.3871008422268005
1101	44-45	0.33312636830775944
1101	46-47	0.34272848985546034
1101	48-49	0.31393284819588985
1101	50-51	0.26994755063202547
1101	52-53	0.3010378479144933
1101	54-55	0.3305752084048166
1101	56-57	0.33600476865803586
1101	58-59	0.36923380372248005
1101	60-61	0.3300416013757115
1101	62-63	0.31492888679430564
1101	64-65	0.23050882936012584
1101	66-67	0.2517999081310762
1101	68-69	0.21074234326824381
1101	70-71	0.24605497937437804
1101	72-73	0.21905172386158966
1101	74-75	0.21448358554599167
1101	76-77	0.21874156518548915
1101	78-79	0.2279527740702889
1101	80-81	0.19451459991261544
1101	82-83	0.19380027876063366
1101	84-85	0.22555132059277838
1101	86-87	0.14910934274562493
1101	88-89	0.15157077200941416
1101	90-91	0.20519887296245543
1101	92-93	0.24127646938824654
1101	94-95	0.1780479472268297
1101	96-97	0.1733568286883127
1101	98-99	0.09372259694407603
1101	100	0.09885374149672543
1102	1	0.18249855258095948
1102	2	0.22916355430855262
1102	3	0.28366402217979214
1102	4	0.21568028986264665
1102	5	0.1667756167805834
1102	6	0.13262402429563025
1102	7	0.21488067772618535
1102	8	0.29564760144708657
1102	9	0.310632198878416
1102	10-11	0.3368415584919404
1102	12-13	0.30557940333827105
1102	14-15	0.3911552243452121
1102	16-17	0.34896692590086786
1102	18-19	0.2925363766648914
1102	20-21	0.2864300993293796
1102	22-23	0.2867405484632002
1102	24-25	0.281159567899536
1102	26-27	0.3039849790668825
1102	28-29	0.3614513621935629
1102	30-31	0.3113923241215417
1102	32-33	0.2764570774474464
1102	34-35	0.3056282986528771
1102	36-37	0.3389843899484717
1102	38-39	0.2931520270775536
1102	40-41	0.33523226973444054
1102	42-43	0.24432500282337344
1102	44-45	0.22591053913179593
1102	46-47	0.24251392591803267
1102	48-49	0.24301477227749047
1102	50-51	0.16914944603306026
1102	52-53	0.13461823298649733
1102	54-55	0.15068163903314513
1102	56-57	0.18478408189420037
1102	58-59	0.16099276053990508
1102	60-61	0.17027154062287764
1102	62-63	0.2203917452643367
1102	64-65	0.10971613990020757
1102	66-67	0.07061860822928168
1102	68-69	0.08858119664638053
1102	70-71	0.07300682364949296
1102	72-73	0.15033143689220907
1102	74-75	0.11638326887239714
1102	76-77	0.042773573536699416
1102	78-79	0.036853813626848364
1102	80-81	0.06925134976912517
1102	82-83	0.06307261191555114
1102	84-85	0.0984624088093824
1102	86-87	0.11064994127487893
1102	88-89	0.04065613329961337
1102	90-91	0.10466441659533388
1102	92-93	0.062495585409166665
1102	94-95	0.05963227178121144
1102	96-97	0.0663640996015431
1102	98-99	0.12050544133770558
1102	100	0.020057212645216538
1103	1	0.14356221252784707
1103	2	0.14037028497863702
1103	3	0.2051814912047938
1103	4	0.17501125126806016
1103	5	0.19724614031061094
1103	6	0.1711014113305609
1103	7	0.22077217691812479
1103	8	0.20340525435732815
1103	9	0.41850784273020736
1103	10-11	0.34610746895172895
1103	12-13	0.31953616909210325
1103	14-15	0.34399805322387067
1103	16-17	0.3119678068648781
1103	18-19	0.2770519937261682
1103	20-21	0.2765525495818508
1103	22-23	0.3262967433536943
1103	24-25	0.24542966745132588
1103	26-27	0.26142795686494935
1103	28-29	0.30188468716790595
1103	30-31	0.30984824789331356
1103	32-33	0.31936258985211907
1103	34-35	0.3132845761515668
1103	36-37	0.3088944922745469
1103	38-39	0.3009676415288496
1103	40-41	0.26795678240284815
1103	42-43	0.2720013732091928
1103	44-45	0.24994988010164576
1103	46-47	0.19709730561519478
1103	48-49	0.11488352311545924
1103	50-51	0.19390024242440518
1103	52-53	0.22892352135816196
1103	54-55	0.24443201453127017
1103	56-57	0.1899214551057824
1103	58-59	0.2313794541662446
1103	60-61	0.1874876161174086
1103	62-63	0.21078913698940482
1103	64-65	0.24584139830579232
1103	66-67	0.20471328455870008
1103	68-69	0.22905066445034095
1103	70-71	0.1806157974664231
1103	72-73	0.1658550532720895
1103	74-75	0.10041861160807741
1103	76-77	0.13990495509761303
1103	78-79	0.1517081197960195
1103	80-81	0.2613272956513484
1103	82-83	0.12935024529402028
1103	84-85	0.14668299061672485
1103	86-87	0.12414146660009351
1103	88-89	0.11388202587298224
1103	90-91	0.19120226605169677
1103	92-93	0.14376626155840455
1103	94-95	0.11047414486170481
1103	96-97	0.1360853057627125
1103	98-99	0.09161249894754775
1103	100	0.10500387221111396
1104	1	0.20620148040227448
1104	2	0.09974806978720352
1104	3	0.14277563772155943
1104	4	0.22358066335052484
1104	5	0.1958879144707737
1104	6	0.18949304978438164
1104	7	0.16040810523087146
1104	8	0.21831949368395698
1104	9	0.29432131229047087
1104	10-11	0.25685518906710314
1104	12-13	0.22233555572579178
1104	14-15	0.29917096921901987
1104	16-17	0.22611013581043693
1104	18-19	0.27279370515168466
1104	20-21	0.24930606909941844
1104	22-23	0.2880548073223679
1104	24-25	0.17794842003480227
1104	26-27	0.20565439403605268
1104	28-29	0.2712158547105119
1104	30-31	0.3018167108089145
1104	32-33	0.29570499770806435
1104	34-35	0.24402402188693628
1104	36-37	0.23799698914207568
1104	38-39	0.26714451167124764
1104	40-41	0.22989912904822063
1104	42-43	0.22913971277318979
1104	44-45	0.16285820740028356
1104	46-47	0.202000816042613
1104	48-49	0.18773841618732945
1104	50-51	0.14487516081332785
1104	52-53	0.14393609127592555
1104	54-55	0.15955896680009118
1104	56-57	0.11452199386008033
1104	58-59	0.16249994192069295
1104	60-61	0.1362812620383167
1104	62-63	0.1700162718721856
1104	64-65	0.17240934966260824
1104	66-67	0.12973051294827087
1104	68-69	0.1704359866115439
1104	70-71	0.15973293310503323
1104	72-73	0.1914409747988728
1104	74-75	0.14478935446256003
1104	76-77	0.14950943033034747
1104	78-79	0.23362835665324866
1104	80-81	0.20317590766491378
1104	82-83	0.1996591752853618
1104	84-85	0.17853726130189784
1104	86-87	0.2173509935236737
1104	88-89	0.19123340075762485
1104	90-91	0.2089768907662517
1104	92-93	0.1833152768498323
1104	94-95	0.14325065554180583
1104	96-97	0.1576422975440792
1104	98-99	0.13494018738613534
1104	100	0.10582950199987451
1105	1	0.08559440157531029
1105	2	0.0915179683007139
1105	3	0.15221714812213705
1105	4	0.139829482750784
1105	5	0.06558924471017491
1105	6	0.053693841120093566
1105	7	-0.02179572204689606
1105	8	0.12150536077781737
1105	9	0.0832363568239387
1105	10-11	0.09088684843156614
1105	12-13	0.0867881855095689
1105	14-15	0.16889115709219027
1105	16-17	0.1673554336731513
1105	18-19	0.10714811314641537
1105	20-21	0.0825403884404281
1105	22-23	0.1507045925256989
1105	24-25	0.19969341779631833
1105	26-27	0.17090246894788663
1105	28-29	0.17291820134441593
1105	30-31	0.20612207297480012
1105	32-33	0.20844597760641648
1105	34-35	0.18361866038312513
1105	36-37	0.20080238406909956
1105	38-39	0.17570820606891147
1105	40-41	0.12483016969510174
1105	42-43	0.06772864124179989
1105	44-45	0.03406575118435029
1105	46-47	0.019322237561183897
1105	48-49	0.11222555589751693
1105	50-51	0.10960928529973302
1105	52-53	0.06652484258322744
1105	54-55	0.0894938394733984
1105	56-57	0.02520445527898829
1105	58-59	0.12331341366814286
1105	60-61	0.15063121360433485
1105	62-63	0.08169001471752324
1105	64-65	0.11167646804494069
1105	66-67	0.10062300183402328
1105	68-69	0.05836801420213078
1105	70-71	0.040989472491808954
1105	72-73	-0.007531991892747669
1105	74-75	0.030808839009907274
1105	76-77	-0.006527512367782151
1105	78-79	-0.00894021744252882
1105	80-81	-7.803143154632153E-4
1105	82-83	0.019852778803382876
1105	84-85	-0.0014708844653341657
1105	86-87	-0.009082135275100711
1105	88-89	-0.016967067304371142
1105	90-91	-0.031446890034075636
1105	92-93	-0.03450587196360999
1105	94-95	-0.04848641333810377
1105	96-97	0.00968244923615913
1105	98-99	0.05167496755201739
1105	100	0.003480356791754957
1106	1	0.07758712575194338
1106	2	0.09323114089511364
1106	3	0.1197494313632319
1106	4	0.07476569029235947
1106	5	0.07080422981022139
1106	6	0.01519325415207362
1106	7	0.008334442949170295
1106	8	0.04044443220730898
1106	9	0.027542122010927983
1106	10-11	0.10158417933962482
1106	12-13	0.08710521274230132
1106	14-15	0.08225693492157404
1106	16-17	0.11323375713768513
1106	18-19	0.09938755739734262
1106	20-21	0.11139792936693027
1106	22-23	0.10524875703154635
1106	24-25	0.09726040310276574
1106	26-27	0.11951212876444828
1106	28-29	0.20839558049995333
1106	30-31	0.17490117949336792
1106	32-33	0.13680459571600068
1106	34-35	0.15439249063971516
1106	36-37	0.1829001819714975
1106	38-39	0.14419576363692954
1106	40-41	0.07073171387250454
1106	42-43	0.030767394031514073
1106	44-45	0.0848475151844994
1106	46-47	0.09334438754118679
1106	48-49	0.09331067271060078
1106	50-51	0.143467019732185
1106	52-53	0.02618885176831043
1106	54-55	0.10903116676934133
1106	56-57	0.03837221202911678
1106	58-59	0.048493696702834654
1106	60-61	0.09702394036104067
1106	62-63	0.09259665455137167
1106	64-65	0.06946217956627976
1106	66-67	0.013433740123694804
1106	68-69	0.014554076614246014
1106	70-71	0.003996296801311416
1106	72-73	0.01041826418011027
1106	74-75	0.03784858513012068
1106	76-77	0.0771915428072063
1106	78-79	0.0959419374396333
1106	80-81	0.13772962543968958
1106	82-83	0.07344070145260417
1106	84-85	0.0735936175148737
1106	86-87	0.041461616418075664
1106	88-89	0.020555669643648855
1106	90-91	0.046101905713953784
1106	92-93	0.06781136167546009
1106	94-95	-0.025755270492012983
1106	96-97	-0.036492583337341955
1106	98-99	-0.0027547059389547712
1106	100	-0.09101246413784736
1107	1	0.05117297020260736
1107	2	0.027031972233029222
1107	3	0.09139894261532078
1107	4	0.0523019447107842
1107	5	0.0754346368755563
1107	6	0.043599718270826315
1107	7	0.0039471141145952515
1107	8	0.02471017009744969
1107	9	0.053720973686566253
1107	10-11	0.10438035642911814
1107	12-13	0.0524994763033817
1107	14-15	0.08610187277854209
1107	16-17	0.007617561597051292
1107	18-19	0.0028829083318271387
1107	20-21	0.027252953156462922
1107	22-23	0.017017837895224375
1107	24-25	0.047609212589556194
1107	26-27	0.03524587258382894
1107	28-29	0.0719027484571626
1107	30-31	0.07613160107083417
1107	32-33	0.07132792397008103
1107	34-35	0.05303742020404201
1107	36-37	0.05811164448354589
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2216	9	-0.03346377888130547
2216	10-11	-0.040573611536558474
2216	12-13	-0.12506393184970932
2216	14-15	-0.27147183220895954
2216	16-17	-0.1956234299032218
2216	18-19	-0.2655993970642072
2216	20-21	-0.3115924530180223
2216	22-23	-0.21439674004378162
2216	24-25	-0.2869236989194306
2216	26-27	-0.28591391314385106
2216	28-29	-0.29285187433136173
2216	30-31	-0.26178278870301597
2216	32-33	-0.2719638532461417
2216	34-35	-0.22627161251917016
2216	36-37	-0.1844334824568179
2216	38-39	-0.14391703467072148
2216	40-41	-0.18202807176828628
2216	42-43	-0.15928566377264985
2216	44-45	-0.08660724078660564
2216	46-47	-0.061203195300294055
2216	48-49	-0.04487368110237355
2216	50-51	-0.0509044648619863
2216	52-53	0.061881699389942924
2216	54-55	0.07160707574399794
2216	56-57	0.07919561086109894
2216	58-59	0.01236634475549181
2216	60-61	-0.0487215057613426
2216	62-63	0.008847106420006412
2216	64-65	-0.04523333201509416
2216	66-67	-0.07547315742388605
2216	68-69	-0.025163557530433422
2216	70-71	-0.02145175532268695
2216	72-73	-0.017337572773122645
2216	74-75	-0.03794361411371483
2216	76-77	-0.0152921717479515
2216	78-79	-0.0443125695163431
2216	80-81	-0.05634633541311729
2216	82-83	0.0038891633148949722
2216	84-85	0.013511263423012565
2216	86-87	0.008184985577074144
2216	88-89	-0.03023757046761233
2216	90-91	-0.060363401018115326
2216	92-93	-0.1135606304654928
2216	94-95	-0.011230213343431217
2216	96-97	-0.05195594116905511
2216	98-99	-0.03038955444483804
2216	100	-0.05492985296825026
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	95.0
1	46.0
2	46.0
3	44.0
4	34.0
5	60.0
6	125.0
7	676.0
8	2337.0
9	2931.0
10	2669.0
11	2822.0
12	3193.0
13	4031.0
14	5384.0
15	6310.0
16	6836.0
17	7566.0
18	8215.0
19	9251.0
20	10552.0
21	11918.0
22	13863.0
23	17003.0
24	22434.0
25	30227.0
26	33901.0
27	30859.0
28	31101.0
29	38517.0
30	44302.0
31	47554.0
32	59098.0
33	80750.0
34	128455.0
35	203025.0
36	398593.0
37	833099.0
38	894009.0
39	300034.0
40	352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.38540439919462	21.223477567146602	13.085377806481024	24.30574022717775
2	15.405177991561386	21.259959720656983	39.76333607735092	23.57152621043071
3	17.715300841424618	26.818659158174402	30.78248935194903	24.683550648451952
4	12.531082871659407	17.991674377264196	40.14099609781999	29.336246653256403
5	13.053356156789246	38.5517636283827	35.46597973532285	12.928900479505215
6	28.74722767076956	39.08369829402836	18.775682302082046	13.39339173312004
7	25.258215449065858	33.2062759020379	24.182950551179573	17.35255809771667
8	24.861198947062107	35.09244189613872	21.42250499829593	18.62385415850324
9	25.913469482974815	15.296148556042652	21.717864537262948	37.07251742371959
10-11	24.305054035110103	26.66718305869631	28.773166447579758	20.254596458613836
12-13	24.708150167795427	24.912548327756355	28.030691010976565	22.34861049347165
14-15	22.525153278443145	26.013038103516546	25.6258075530234	25.836001065016912
16-17	21.914476741890116	28.4217808789266	26.849713089730464	22.81402928945282
18-19	21.54817822521436	28.732256046939934	28.121357688264137	21.598208039581575
20-21	23.12364010705697	27.888653340336	26.99974696840507	21.987959584201956
22-23	23.45919477412205	27.56141237334009	26.566980481172113	22.412412371365743
24-25	23.035573449090172	27.709698275174706	27.093709852883574	22.161018422851548
26-27	22.784323928048593	27.95482328583685	26.528668219760497	22.732184566354057
28-29	22.209202845052012	28.347288198738706	26.865195377481527	22.57831357872775
30-31	22.79767830929398	27.843277523752676	26.99192511537448	22.367119051578864
32-33	23.062992812460227	28.2735718406159	26.09249176854913	22.570943578374745
34-35	23.102941442274087	28.010554980998087	26.922978196063042	21.963525380664787
36-37	22.940580493851165	27.579017401448503	26.83216473047198	22.64823737422836
38-39	22.9134011720768	27.63565761358529	27.1716694081758	22.279271806162114
40-41	22.778760384458113	27.01918584268666	27.54952003494853	22.652533737906698
42-43	22.570817729907354	27.360945447607428	26.9972182709409	23.071018551544324
44-45	22.587734089736603	27.982925630270227	27.078088550468106	22.351251729525064
46-47	22.592504424938745	27.560786603787545	26.770655322376026	23.076053648897688
48-49	23.088236299457883	27.177703894951687	27.0556832933409	22.678376512249535
50-51	22.884184460193392	27.208349474739645	26.750632363329764	23.156833701737195
52-53	22.437760366739777	27.66297579263452	26.7736385122951	23.125625328330603
54-55	22.565426111011007	27.49326284783805	26.737614016558098	23.203697024592845
56-57	23.149916164942663	27.208259166097942	26.556114185839746	23.085710483119648
58-59	22.520955905923806	27.293800783147887	27.28580947817386	22.899433832754447
60-61	22.670085423858183	27.72699794701372	26.741798499844805	22.86111812928329
62-63	23.025017668359872	27.189524531218762	26.43879394529766	23.346663855123705
64-65	22.301008027178497	27.341812382762587	26.821991160183057	23.535188429875863
66-67	22.976965575602335	27.060871863940182	26.68770856739599	23.27445399306149
68-69	22.403523343992315	27.12299084356577	26.956960489850317	23.5165253225916
70-71	22.56612697923627	26.993016363823653	26.763738085232387	23.677118571707688
72-73	23.003337930057977	27.027692619955296	26.89123416207564	23.077735287911093
74-75	22.867035186201424	27.463729935466542	26.837037064046676	22.832197814285358
76-77	22.7114204163649	27.26683108320294	26.659513894048114	23.362234606384046
78-79	22.73795942601289	27.3489657326637	26.509537459099292	23.403537382224123
80-81	22.464492664554683	27.43182003610254	27.063760130383606	23.039927168959174
82-83	23.080399886114925	26.63733346054754	27.734176188234894	22.54809046510264
84-85	23.274809219450766	27.21087959970229	26.554513383693635	22.959797797153307
86-87	22.28026251823043	27.59719859017987	27.083951142440448	23.038587749149247
88-89	23.02115671066434	26.791582890811988	26.815859215546574	23.3714011829771
90-91	22.92297228543349	26.7370693132301	27.062937158550888	23.277021242785523
92-93	23.260918213610147	26.856951260512886	26.957736991173732	22.924393534703245
94-95	22.32227557275521	26.99754058161872	27.30238509496442	23.37779875066165
96-97	22.474389155999784	26.96646556376064	27.03204986412185	23.527095416117724
98-99	22.208821436975708	27.42398708825311	26.8274234249946	23.539768049776583
100	22.902930505875567	26.812975736652184	26.353284966045777	23.93080879142648
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1121.0
1	843.0
2	568.0
3	567.0
4	503.5
5	419.5
6	364.0
7	324.0
8	329.0
9	325.0
10	271.0
11	262.0
12	277.5
13	275.5
14	307.0
15	382.0
16	475.5
17	611.0
18	772.0
19	943.0
20	1305.5
21	2030.5
22	2856.0
23	3890.0
24	5578.5
25	7713.0
26	10556.5
27	14219.0
28	18201.5
29	24324.0
30	32138.5
31	40615.5
32	51724.0
33	61819.5
34	69879.0
35	77676.0
36	86586.5
37	98275.5
38	101344.0
39	99554.5
40	99426.0
41	96937.0
42	96513.5
43	101085.0
44	108350.5
45	122563.5
46	136607.0
47	144810.0
48	148542.0
49	143070.5
50	136015.5
51	138118.5
52	139692.5
53	136173.0
54	129872.0
55	112471.0
56	99327.5
57	83065.0
58	65133.0
59	59122.0
60	48897.0
61	36115.0
62	27185.5
63	21460.5
64	15118.5
65	8135.5
66	4934.0
67	3398.0
68	2443.0
69	1954.0
70	1614.5
71	1203.5
72	808.0
73	661.5
74	561.5
75	354.5
76	233.0
77	168.5
78	111.0
79	78.5
80	56.0
81	44.0
82	31.0
83	28.0
84	27.0
85	16.0
86	14.0
87	14.5
88	10.0
89	8.0
90	8.5
91	7.0
92	5.0
93	5.0
94	5.0
95	7.5
96	6.5
97	4.5
98	5.0
99	5.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05898581454944952
2	0.009324739993141607
3	0.012149498362399489
4	0.009446234976765604
5	0.011602770936091512
6	0.012453235821459477
7	0.003189243320129866
8	0.005922880451669751
9	0.007684557714217677
10-11	0.007426380874016688
12-13	0.0064696078779777275
14-15	0.010645997940052552
16-17	0.010539689829381558
18-19	0.008504648853679643
20-21	0.007228951525627696
22-23	0.002596955274962891
24-25	0.003903026348920836
26-27	0.005421713644220772
28-29	0.006545542242742726
30-31	0.009704411816966593
32-33	0.011238285985219528
34-35	0.012498796440318474
36-37	0.019348076142121185
38-39	0.019393636760980187
40-41	0.01880134871581321
42-43	0.02548357281513293
44-45	0.028885432356604786
46-47	0.031239397664319688
48-49	0.032484721246465634
50-51	0.0331681305293506
52-53	0.03177093821767466
54-55	0.03760269743162642
56-57	0.0404274558008843
58-59	0.03766344492343841
60-61	0.0381949854767934
62-63	0.03410971665243656
64-65	0.03600807577156148
66-67	0.03840760169813538
68-69	0.041080491337863276
70-71	0.04064007202222629
72-73	0.03661555068968146
74-75	0.039819980882764325
76-77	0.03904545036216136
78-79	0.03849872293585339
80-81	0.042902916092223194
82-83	0.038878394759678365
84-85	0.03543097459934751
86-87	0.03392747417700057
88-89	0.03172537759881567
90-91	0.03357817609908159
92-93	0.0357347120584075
94-95	0.03933400094826835
96-97	0.03441345411149656
98-99	0.03190762007425166
100	0.029584028512442757
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3292317.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.2653593947129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.3232122620433	33.16702247696106
2	14.714872769537918	14.4986699087752
3	6.5958189631720465	9.748361751694008
4	3.560526283932454	7.016424280490158
5	2.120349452224407	5.222988890310902
6	1.3997167095587393	4.137452804831777
7	0.9581960144541343	3.304410971586511
8	0.6689659132478386	2.6365476911173684
9	0.5096155931303595	2.2595755813845257
>10	2.1134162315014553	16.45917064774079
>50	0.03015004459204557	0.9548601021121178
>100	0.004808046465364384	0.36105676003905335
>500	1.1723871332180513E-4	0.047072829876195425
>1k	2.3447742664361025E-4	0.18638530308034681
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.0373745905998723E-4	0.0
3	0.0	0.0	0.0	3.0373745905998723E-4	0.0
4	0.0	0.0	0.0	4.5560618858998087E-4	0.0
5	0.0	0.0	0.0	4.8597993449597956E-4	0.0
6	0.0	0.0	0.0	4.8597993449597956E-4	0.0
7	0.0	0.0	0.0	4.8597993449597956E-4	0.0
8	0.0	0.0	0.0	4.8597993449597956E-4	0.0
9	0.0	0.0	0.0	5.771011722139757E-4	0.0
10-11	0.0	0.0	0.0	0.0010023336148979579	0.0
12-13	0.0	0.0	0.0	0.0010175204878509572	0.0
14-15	0.0	0.0	0.0	0.0011845760903339503	0.0
16-17	0.0	0.0	0.0	0.001214949836239949	0.0
18-19	0.0	0.0	0.0	0.0012453235821459478	0.0
20-21	0.0	0.0	0.0	0.0013364448198639439	0.0
22-23	0.0	0.0	0.0	0.00167055602482993	0.0
24-25	0.0	0.0	0.0	0.002353965307714901	0.0
26-27	0.0	0.0	0.0	0.0033259251767068605	0.0
28-29	0.0	0.0	0.0	0.007213764652674697	0.0
30-31	0.0	0.0	0.0	0.018452050637894225	0.0
32-33	0.0	0.0	0.0	0.04718561426496902	0.0
34-35	0.0	0.0	0.0	0.09324739993141608	0.0
36-37	0.0	0.0	0.0	0.16483831903185509	0.0
38-39	0.0	0.0	0.0	0.2561569860982402	0.0
40-41	1.5186872952999361E-5	0.0	0.0	0.35140905325945226	0.0
42-43	3.0373745905998722E-5	0.0	0.0	0.45158166725743604	0.0
44-45	3.0373745905998722E-5	0.0	0.0	0.5730462771355249	0.0
46-47	3.0373745905998722E-5	0.0	0.0	0.7114290634832551	0.0
48-49	3.0373745905998722E-5	0.0	0.0	0.8576330894017801	0.0
50-51	3.0373745905998722E-5	0.0	0.0	1.0035637516071507	0.0
52-53	3.0373745905998722E-5	0.0	0.0	1.1449687256725278	0.0
54-55	3.0373745905998722E-5	0.0	0.0	1.2989939911618475	0.0
56-57	3.0373745905998722E-5	0.0	0.0	1.4776371777079789	0.0
58-59	3.0373745905998722E-5	0.0	0.0	1.6757043747609965	0.0
60-61	3.0373745905998722E-5	0.0	0.0	1.8731185362770353	0.0
62-63	3.0373745905998722E-5	0.0	0.0	2.06585514092355	0.0
64-65	3.0373745905998722E-5	0.0	0.0	2.2726244161786364	0.0
66-67	3.0373745905998722E-5	0.0	0.0	2.501779142166444	0.0
68-69	3.0373745905998722E-5	0.0	0.0	2.7497959643618763	0.0
70-71	3.0373745905998722E-5	0.0	0.0	3.010114153649239	0.0
72-73	3.0373745905998722E-5	0.0	0.0	3.2719965908507596	0.0
74-75	3.0373745905998722E-5	0.0	0.0	3.5315250627445653	0.0
76-77	3.0373745905998722E-5	0.0	0.0	3.8209564874828272	0.0
78-79	3.0373745905998722E-5	0.0	0.0	4.130723135105155	0.0
80-81	3.0373745905998722E-5	0.0	0.0	4.462009581701883	0.0
82-83	3.0373745905998722E-5	0.0	0.0	4.795088079307066	0.0
84-85	3.0373745905998722E-5	0.0	0.0	5.133132684367879	0.0
86-87	3.0373745905998722E-5	0.0	0.0	5.488247334627863	0.0
88	3.0373745905998722E-5	0.0	0.0	5.7618692246220515	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3960	0.0	32.52876	1
GTATCAA	5915	0.0	31.871447	1
CAACGCA	5405	0.0	31.561535	5
ATCAACG	5670	0.0	30.251741	3
GTACTAG	2335	0.0	28.589962	1
GCATCGT	2355	0.0	26.540426	8
TATCAAC	7170	0.0	26.151363	2
CTGTGCG	3065	0.0	25.45217	9
AACGCAG	7220	0.0	25.384079	6
GGGCACC	3055	0.0	24.77123	94
GTACATG	11340	0.0	24.70844	1
ACGCAGA	6865	0.0	24.642385	7
TACATGG	11060	0.0	24.559147	2
CGCAGAG	7000	0.0	23.967203	8
CGTTAAC	570	0.0	23.918573	1
TAGTACT	3205	0.0	23.900486	4
ACATGGG	11530	0.0	22.905912	3
CTAGTAC	3375	0.0	22.835506	3
GCTATTG	2585	0.0	22.542824	8
CATGGGG	9230	0.0	22.097097	4
>>END_MODULE