FastQCFastQC Report
Mon 16 Jan 2017
ERR1146531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3292317
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119960.36436345588836067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117540.35701300937910896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61060.18546209250202822No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT43030.1306982286335125No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79300.055.832091
GTATCAA142450.041.815641
CAACGCA170750.034.306395
ATCAACG171700.034.0349463
GTGGTAT14850.033.9019621
TATCAAC182750.032.5167272
AACGCAG191300.031.4239716
TCAACGC196400.029.9935074
ACGCAGA195900.029.8440997
CGCAGAG198400.029.4443538
GCAGAGT210100.027.5586019
GTACTAG21000.026.6621481
GAGTACT152600.024.65354312-13
CAGAGTA211400.023.76540810-11
AGAGTAC202650.023.0985810-11
TACATGG107250.022.1980132
GTACATG110150.022.1692161
AGTACTT161550.022.1095112-13
TAACGCC10000.022.0960944
CTAACGC10250.022.0161613
Produced by FastQC (version 0.11.5)